##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	629634
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5611863400007	31.0	31.0	34.0	30.0	34.0
2	31.694085770463477	31.0	31.0	34.0	30.0	34.0
3	31.6618464060073	33.0	31.0	34.0	30.0	34.0
4	35.398455293075024	37.0	35.0	37.0	33.0	37.0
5	34.215385446148076	37.0	35.0	37.0	32.0	37.0
6	34.717380255831166	37.0	35.0	37.0	32.0	37.0
7	35.45046010857101	37.0	35.0	37.0	33.0	37.0
8	35.29772534520055	37.0	35.0	37.0	33.0	37.0
9	37.16487991436295	39.0	37.0	39.0	34.0	39.0
10	36.667440131886146	39.0	35.0	39.0	32.0	39.0
11	36.80376059742644	39.0	37.0	39.0	33.0	39.0
12	36.840712223291625	39.0	37.0	39.0	33.0	39.0
13	36.935489506602245	39.0	37.0	39.0	33.0	39.0
14	37.94417074046192	40.0	37.0	41.0	33.0	41.0
15	38.04586632869254	40.0	37.0	41.0	33.0	41.0
16	38.12653223936446	40.0	37.0	41.0	34.0	41.0
17	38.04698126213006	40.0	37.0	41.0	33.0	41.0
18	37.92561551631583	39.0	37.0	41.0	33.0	41.0
19	37.830137826102145	39.0	37.0	41.0	33.0	41.0
20	37.880432124059375	39.0	36.0	41.0	34.0	41.0
21	37.75160648884908	39.0	36.0	41.0	33.0	41.0
22	37.88791107214668	40.0	36.0	41.0	34.0	41.0
23	37.94056388314481	40.0	36.0	41.0	34.0	41.0
24	37.841082279546534	40.0	36.0	41.0	33.0	41.0
25	37.5465667355956	39.0	35.0	41.0	33.0	41.0
26	37.6742599669014	39.0	36.0	41.0	33.0	41.0
27	37.70686779938821	40.0	36.0	41.0	33.0	41.0
28	37.586054755619934	40.0	36.0	41.0	33.0	41.0
29	37.574263778639654	40.0	36.0	41.0	33.0	41.0
30	37.25553099102018	39.0	35.0	41.0	33.0	41.0
31	37.065506310015024	39.0	35.0	41.0	32.0	41.0
32	37.09252041662299	39.0	35.0	41.0	32.0	41.0
33	36.98076342764209	39.0	35.0	41.0	32.0	41.0
34	36.735617835123264	39.0	35.0	41.0	31.0	41.0
35	36.67899287522592	39.0	35.0	41.0	31.0	41.0
36	36.5032209188195	39.0	35.0	41.0	30.0	41.0
37	36.49384563095386	39.0	35.0	41.0	30.0	41.0
38	36.3410679855281	39.0	35.0	41.0	30.0	41.0
39	36.25600904652544	39.0	35.0	41.0	30.0	41.0
40	36.158536546628675	39.0	35.0	41.0	30.0	41.0
41	35.98222141752193	39.0	35.0	40.0	29.0	41.0
42	36.162556342255975	39.0	35.0	41.0	30.0	41.0
43	36.15711667413132	39.0	35.0	41.0	30.0	41.0
44	36.24971332551927	39.0	35.0	41.0	30.0	41.0
45	36.22698742444023	39.0	35.0	41.0	30.0	41.0
46	36.11009570639451	39.0	35.0	41.0	30.0	41.0
47	36.02837997948014	39.0	35.0	41.0	29.0	41.0
48	36.01720364529235	39.0	35.0	41.0	29.0	41.0
49	36.01041716298675	39.0	35.0	41.0	29.0	41.0
50	35.92137019284219	39.0	35.0	41.0	29.0	41.0
51	35.044524279184415	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	30.0
10	45.0
11	31.0
12	31.0
13	32.0
14	37.0
15	57.0
16	63.0
17	121.0
18	226.0
19	324.0
20	681.0
21	1264.0
22	1920.0
23	2931.0
24	4192.0
25	6056.0
26	7622.0
27	8147.0
28	7995.0
29	8311.0
30	9581.0
31	12298.0
32	15935.0
33	22075.0
34	37177.0
35	47587.0
36	52328.0
37	78865.0
38	137044.0
39	166572.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.909426746331995	20.409158336430373	23.631665380205007	14.049749537032625
2	33.89334121092571	26.44869876785561	23.565436428147144	16.09252359307153
3	29.255885165032385	26.063395559960227	29.666123493966335	15.014595781041049
4	25.919820085954697	23.991715822207823	32.43995718147368	17.6485069103638
5	25.52911691554141	29.295908416635697	26.978371561891514	18.196603105931384
6	23.598789137816574	34.440008004650316	27.364786526775873	14.596416330757233
7	73.82892283453562	4.926195218174368	16.38189805506056	4.862983892229454
8	73.95550430885245	7.295349361692666	12.881134119186703	5.868012210268188
9	67.97615757725917	7.1609855884529745	15.187076936760086	9.67577989752777
10	35.09832696455401	29.814940108062782	21.1059123236674	13.98082060371581
11	26.139312680064926	24.380989590778135	30.785186314589108	18.694511414567828
12	23.80271713408107	20.56686900643866	34.356785052903746	21.273628806576518
13	22.99240511154099	23.948357299637568	35.44964217307198	17.609595415749467
14	18.769316777683542	28.99366933805989	31.46907568523936	20.76793819901721
15	16.901723858622628	25.37744149775902	37.59819196549106	20.122642678127292
16	19.226566545008687	25.957619823580046	32.347840173815264	22.467973457596
17	19.435259214083104	25.5064053084808	31.076784290556102	23.981551186879997
18	21.05175387606133	24.33000759171201	33.02791780621758	21.59032072600908
19	20.687574050956588	26.401369684610426	30.110985111985695	22.800071152447295
20	22.380462300320502	25.954284552613103	31.983342703856525	19.681910443209865
21	21.706261097717086	27.927653208054203	31.46383454514845	18.90225114908026
22	19.668569359342094	24.86730386224378	30.875556275550558	24.58857050286357
23	18.437060260405254	27.81885984556107	30.73690429678194	23.007175597251734
24	20.082460604096983	25.387606133086845	31.016114123443145	23.513819139373034
25	18.660999882471405	28.959681338682476	29.28050264121696	23.098816137629164
26	18.205020694562236	28.03088778560243	30.31364252883421	23.45044899100112
27	20.381523234132846	28.42063802145373	29.143597709145315	22.054241035268106
28	17.756506160721944	28.44414374064933	31.326611968222807	22.47273813040592
29	20.052919632675493	26.624674016968587	30.42783585384525	22.89457049651067
30	21.419427794560015	30.102726345781832	28.385538265087334	20.092307594570812
31	20.963607429077847	30.507247067343883	26.34419361089141	22.184951892686865
32	23.626424240114098	29.83876347211237	25.077108288307176	21.457703999466357
33	23.160439239304104	27.022524196596752	25.805150293662667	24.011886270436474
34	20.782073395019964	26.992824402748262	29.28050264121696	22.94459956101481
35	20.96853092431476	27.14481746538465	26.52779233650025	25.358859273800334
36	21.030154025989702	30.554258505735078	25.630127979111673	22.785459489163546
37	20.40455248604745	28.979375319630137	28.725100614007502	21.890971580314915
38	21.732466798171636	28.39649701255015	25.559928466378878	24.311107722899337
39	24.06334473678359	24.566017718229958	28.017229056880666	23.35340848810579
40	23.742999901530094	23.863228478767027	29.087692214842274	23.306079404860604
41	19.12904957483236	25.584704764990455	27.631925848985283	27.654319811191897
42	19.817544795865537	25.9488845900952	27.956558889767706	26.27701172427156
43	21.824742628257052	25.893137918219157	26.92881896466836	25.353300488855428
44	21.813625058367244	25.750674201202607	27.36669239589984	25.069008344530314
45	22.29215703091002	24.202155537979205	27.214858155690447	26.290829275420325
46	24.06000946581665	26.114695203880352	26.827966723525094	22.997328606777906
47	19.155572920140905	25.077267110734176	31.84278485596394	23.924375113160977
48	20.262247591457893	24.3131724144503	29.227614773026872	26.19696522106494
49	21.406722000400233	22.535790633923835	31.600580654793102	24.45690671088283
50	21.45230403694845	22.54484351226268	30.22914899767166	25.773703453117207
51	20.521128147463447	22.432873701229603	27.612549512891615	29.43344863841533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	208.0
1	405.0
2	602.0
3	5071.5
4	9541.0
5	6237.5
6	2934.0
7	2877.5
8	2821.0
9	2882.5
10	2944.0
11	3021.5
12	3099.0
13	3063.5
14	3028.0
15	2951.0
16	2874.0
17	2730.0
18	2586.0
19	2593.5
20	2601.0
21	2522.5
22	2444.0
23	2506.0
24	2568.0
25	2863.0
26	3740.5
27	4323.0
28	4679.0
29	5035.0
30	5778.5
31	6522.0
32	7867.5
33	9213.0
34	11120.0
35	13027.0
36	14792.0
37	16557.0
38	18475.5
39	20394.0
40	23378.0
41	26362.0
42	31001.0
43	35640.0
44	41199.0
45	46758.0
46	63676.0
47	80594.0
48	78831.5
49	77069.0
50	74417.0
51	71765.0
52	59736.5
53	47708.0
54	40258.0
55	32808.0
56	28865.0
57	24922.0
58	22218.0
59	19514.0
60	17568.5
61	15623.0
62	13747.0
63	11871.0
64	10268.5
65	8666.0
66	6979.0
67	5292.0
68	4207.5
69	3123.0
70	2707.0
71	2291.0
72	1821.0
73	1351.0
74	1082.5
75	669.0
76	524.0
77	391.5
78	259.0
79	179.0
80	99.0
81	89.0
82	79.0
83	47.0
84	15.0
85	10.5
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	629634.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.56872228173624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.84376000739337	30.01040676535202
2	9.925206613993593	7.854554881959283
3	3.726111589558908	4.4231242403404565
4	1.9202138438988812	3.0392163324312023
5	1.2726385744675601	2.5178341159065796
6	0.9208735939427313	2.186267489718257
7	0.727761543591044	2.0157616083977192
8	0.5967385438721109	1.8889745373826587
9	0.49830461741237375	1.7745549316287343
>10	4.44481952675172	32.90308755969019
>50	0.077743687350207	2.0798474328442973
>100	0.03682595713460369	2.447050761258259
>500	0.0032734184119647724	0.9097511067559982
>1k	0.004910127617947159	4.055751063220814
>5k	8.183546029911931E-4	1.8938171731135278
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6579	1.0448927472150487	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	5118	0.8128531813720351	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	4498	0.7143832766337269	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	4198	0.6667365485345454	No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	3710	0.589231204159877	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2308	0.36656216150970244	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	2031	0.3225683492314583	TruSeq Adapter, Index 22 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT	1507	0.23934539748488803	No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	1236	0.1963045197686275	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAAGGTCCT	1198	0.19026926754273119	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT	1141	0.1812163892038867	No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG	1129	0.17931052007991943	TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC	1086	0.17248115571903677	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT	1008	0.16009300641324958	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	827	0.13134614712674347	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC	813	0.12912263314878167	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA	744	0.11816388568596993	No Hit
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	739	0.11736977355098357	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT	716	0.11371685773004636	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	697	0.1106992316170982	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3340035639752618	0.0
2	0.0	0.0	0.0	1.6611237639644618	0.0
3	0.0	0.0	0.0	2.2513079026863227	0.0
4	0.0	0.0	0.0	3.1226077371933534	0.0
5	0.0	0.0	0.0	5.507326478557384	0.0
6	0.0	0.0	0.0	6.935298919689852	0.0
7	0.0	0.0	0.0	8.186660822001354	0.0
8	0.0	0.0	0.0	9.93068989285839	0.0
9	0.0	0.0	0.0	10.616485132632608	0.0
10	0.0	0.0	0.0	12.775199560379521	0.0
11	0.0	0.0	0.0	14.846561653277936	0.0
12	0.0	0.0	0.0	17.144722171928453	0.0
13	0.0	0.0	0.0	17.957734175727484	0.0
14	0.0	0.0	0.0	18.3119081879314	0.0
15	0.0	0.0	0.0	18.914162831105056	0.0
16	0.0	0.0	0.0	19.858203337176835	0.0
17	0.0	0.0	0.0	21.047465670532404	0.0
18	0.0	0.0	0.0	22.396821010301224	0.0
19	0.0	0.0	0.0	23.36500252527659	0.0
20	0.0	0.0	0.0	24.122267857199578	0.0
21	0.0	0.0	0.0	24.959420869902196	0.0
22	0.0	0.0	0.0	25.850097040502895	0.0
23	0.0	0.0	0.0	26.743155547508554	0.0
24	0.0	0.0	0.0	27.418150862246957	0.0
25	0.0	0.0	0.0	28.05137587868508	0.0
26	0.0	0.0	0.0	28.68809498851714	0.0
27	0.0	0.0	0.0	29.31433181816738	0.0
28	0.0	0.0	0.0	29.8953042561234	0.0
29	0.0	0.0	0.0	30.485964862126252	0.0
30	0.0	0.0	0.0	31.187959989454193	0.0
31	0.0	0.0	0.0	31.791802856897817	0.0
32	0.0	0.0	0.0	32.40358684569131	0.0
33	0.0	0.0	0.0	32.98662397519829	0.0
34	0.0	0.0	0.0	33.570614039267255	0.0
35	0.0	0.0	0.0	34.185892121454685	0.0
36	1.5882242699727142E-4	0.0	0.0	34.745741176620065	0.0
37	1.5882242699727142E-4	0.0	0.0	35.3025726056725	0.0
38	1.5882242699727142E-4	0.0	0.0	35.865756931804825	0.0
39	1.5882242699727142E-4	0.0	0.0	36.422906005711255	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	20	7.0308545E-4	45.0	44
GTTTCGG	35	1.2107557E-7	45.0	2
CGGCCCA	25	3.8887894E-5	45.0	6
GTTAGCG	35	1.2107557E-7	45.0	1
ATTCGCG	25	3.8887894E-5	45.0	12
ATTCGAT	20	7.0308545E-4	45.0	14
CGTTACG	20	7.0308545E-4	45.0	24
CCCGTAT	20	7.0308545E-4	45.0	42
TCTCGCG	25	3.8887894E-5	45.0	32
ACGTACG	20	7.0308545E-4	45.0	1
TTCGCGT	25	3.8887894E-5	45.0	13
TTCGAAG	20	7.0308545E-4	45.0	1
TAATGCG	25	3.8887894E-5	45.0	1
TAGTACG	20	7.0308545E-4	45.0	1
GTACGAG	20	7.0308545E-4	45.0	1
TCGTTAG	30	2.1639316E-6	44.999996	1
TTAGCGT	30	2.1639316E-6	44.999996	1
CTAACGG	30	2.1639316E-6	44.999996	2
TACGTAG	65	0.0	44.999996	1
CGTTTTT	3350	0.0	43.589554	1
>>END_MODULE