FastQCFastQC Report
Sat 18 Jun 2016
SRR3551243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011380
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154481.527417983349483No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC80380.7947556803575314Illumina PCR Primer Index 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC40730.40271707963376774Illumina PCR Primer Index 2 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG38450.3801736241570923No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT34980.3458640669184678Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT34260.33874508097846506No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC32700.3233206114417924No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC28730.2840673139670549Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC20830.20595621823646898Illumina PCR Primer Index 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG20490.20259447487591212Illumina PCR Primer Index 2 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT19960.1973541102256323No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT18570.1836105123692381Illumina PCR Primer Index 2 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT17590.17392078150645654No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT16600.16413217583895273Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC12430.12290138226977003No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT10130.10016017718365007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA453.8380676E-1045.024
GAGTACG406.8139343E-945.01
AAATGCG207.032884E-445.01
CGTTTTT76900.043.5663221
CGTTTCT3900.042.1153831
GCTACGA2400.040.31249610
TAGTGCG403.4576806E-739.3751
GTACGAG403.4576806E-739.3751
TCTAGCG750.039.01
CGAATAT2450.038.5714314
TCGCACA356.2475447E-638.57142637
TATCGCG356.2475447E-638.5714261
CGTTATT3100.038.467741
CGTATTC301.1398251E-437.49999617
TTGGACG301.1398251E-437.4999961
TTGTCGA301.1398251E-437.49999613
GTATACG301.1398251E-437.4999961
ACGTACG301.1398251E-437.4999961
TACGAAT2600.037.21153612
GTTTTTT92150.036.8936542
Produced by FastQC (version 0.11.5)