##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1011380
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.516011785876724	31.0	31.0	34.0	30.0	34.0
2	31.67425794459056	31.0	31.0	34.0	30.0	34.0
3	31.632707785402125	31.0	31.0	34.0	30.0	34.0
4	35.37891890288517	37.0	35.0	37.0	33.0	37.0
5	34.20284166188772	37.0	35.0	37.0	32.0	37.0
6	34.67289742727758	37.0	35.0	37.0	32.0	37.0
7	35.388538432636594	37.0	35.0	37.0	32.0	37.0
8	35.233781565781406	37.0	35.0	37.0	32.0	37.0
9	37.085448594989025	39.0	37.0	39.0	33.0	39.0
10	36.54640392335225	38.0	35.0	39.0	32.0	39.0
11	36.78007474935237	39.0	37.0	39.0	33.0	39.0
12	36.85825011370603	39.0	37.0	39.0	33.0	39.0
13	37.004776641816136	39.0	37.0	39.0	33.0	39.0
14	38.065403705827684	40.0	37.0	41.0	33.0	41.0
15	38.17186616306433	40.0	37.0	41.0	33.0	41.0
16	38.20158891811189	40.0	37.0	41.0	34.0	41.0
17	38.110047657655876	40.0	37.0	41.0	33.0	41.0
18	37.929232336016135	39.0	37.0	41.0	33.0	41.0
19	37.77772943898436	39.0	37.0	41.0	33.0	41.0
20	37.805826692242285	39.0	36.0	41.0	34.0	41.0
21	37.660720006327985	39.0	36.0	41.0	33.0	41.0
22	37.786178291047875	39.0	36.0	41.0	33.0	41.0
23	37.87055310565761	40.0	36.0	41.0	34.0	41.0
24	37.73647887045423	40.0	35.0	41.0	33.0	41.0
25	37.4189404575926	39.0	35.0	41.0	33.0	41.0
26	37.55718622080721	39.0	35.0	41.0	33.0	41.0
27	37.607695426051535	39.0	35.0	41.0	33.0	41.0
28	37.45742747533074	39.0	35.0	41.0	33.0	41.0
29	37.44669263778204	40.0	35.0	41.0	33.0	41.0
30	37.12081907888232	39.0	35.0	41.0	32.0	41.0
31	36.97542763353042	39.0	35.0	41.0	31.0	41.0
32	36.89139294824893	39.0	35.0	41.0	31.0	41.0
33	36.69594316676225	39.0	35.0	41.0	31.0	41.0
34	36.41570922897427	39.0	35.0	41.0	30.0	41.0
35	36.30315212877455	40.0	35.0	41.0	30.0	41.0
36	36.112897229527974	39.0	35.0	41.0	29.0	41.0
37	36.13155886017125	39.0	35.0	41.0	29.0	41.0
38	35.93585101544424	39.0	35.0	41.0	27.0	41.0
39	35.84427613755463	39.0	35.0	41.0	27.0	41.0
40	35.751983428582726	39.0	35.0	41.0	26.0	41.0
41	35.58772667048983	39.0	35.0	41.0	25.0	41.0
42	35.741383060768456	39.0	35.0	41.0	26.0	41.0
43	35.7155213668453	39.0	35.0	41.0	26.0	41.0
44	35.78266724673219	39.0	35.0	41.0	27.0	41.0
45	35.7632076964148	39.0	35.0	41.0	26.0	41.0
46	35.66413415333505	39.0	35.0	41.0	26.0	41.0
47	35.60391741976309	39.0	35.0	41.0	26.0	41.0
48	35.565241551147935	39.0	35.0	41.0	26.0	41.0
49	35.54316280725345	39.0	35.0	41.0	26.0	41.0
50	35.435302260278036	39.0	35.0	41.0	26.0	41.0
51	34.56200043504914	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	34.0
10	54.0
11	38.0
12	56.0
13	59.0
14	69.0
15	113.0
16	138.0
17	254.0
18	452.0
19	725.0
20	1313.0
21	2268.0
22	3500.0
23	4996.0
24	7935.0
25	12112.0
26	16382.0
27	17318.0
28	16114.0
29	15597.0
30	16770.0
31	20043.0
32	25315.0
33	34496.0
34	55813.0
35	71802.0
36	83072.0
37	122178.0
38	209508.0
39	272765.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.00723763570567	19.452233581838676	21.571219521841446	17.96930926061421
2	37.70640115485772	24.309557238624453	22.169510965215842	15.814530641301982
3	28.835946924004823	23.808756352706204	31.869524807688503	15.485771915600466
4	25.593051078724123	23.25090470446321	33.45152168324468	17.704522533567996
5	23.573928691490835	29.52381894045759	29.638909213154303	17.26334315489727
6	23.062548201467305	32.56026419347822	30.220293064921194	14.156894540133283
7	71.09731258280765	5.843995333109217	17.90672150922502	5.151970574858114
8	71.69164903399316	6.643299254483972	15.732069054163619	5.9329826573592515
9	65.22078743894481	7.404931875259546	18.223615258359864	9.150665427435781
10	34.555656627578166	23.391999050801875	26.164745199628232	15.887599121991732
11	25.759852874290573	24.02064505922601	30.85160869307283	19.36789337341059
12	22.384267041072594	20.454725226917677	35.93248828333564	21.22851944867409
13	22.36043821313453	22.633728173386856	36.93755067333742	18.06828294014119
14	18.500464711582197	27.32227253851174	34.56376436156538	19.613498388340684
15	17.6827700765291	25.178963396547292	38.35986473926714	18.77840178765647
16	19.733334651664062	25.04983290158002	33.93294310743736	21.283889339318556
17	20.1750084043584	25.90905495461647	31.904724238169628	22.011212402855502
18	21.01999248551484	24.851094544088276	32.96802388815282	21.160889082244065
19	20.559532519923273	26.996282307342444	30.94870375131009	21.49548142142419
20	21.29891830963634	25.78813106844114	32.55868219660266	20.35426842531986
21	20.675908165081374	27.51211216357848	32.77808538828136	19.033894283058792
22	19.50631810002175	24.510371967015367	32.46405900848346	23.519250924479422
23	19.146908184856333	26.04006407087346	32.31109968557812	22.501928058692084
24	20.106488164685874	24.938005497439143	32.858075105301666	22.097431232573317
25	19.763689216713797	27.410666613933437	30.63210662658941	22.19353754276335
26	18.950938321896814	25.787834444026974	31.777867863710966	23.483359370365246
27	21.33194249441357	24.73046728232712	30.71021772232	23.22737250093931
28	19.214736300895805	24.89944432359746	32.95803753287587	22.92778184263086
29	20.47143506891574	23.20463129585319	31.634499396863692	24.68943423836738
30	20.285550436037887	24.68023888152821	31.189463900808796	23.844746781625105
31	19.9764677964761	25.3979710890071	30.420119045264887	24.205442069251916
32	21.594553975755897	25.778836836797247	29.983191283197215	22.643417904249638
33	20.45670272301212	24.920900156222192	30.59403982677134	24.028357293994347
34	19.385789713065314	26.055982914433745	31.023354228875398	23.534873143625543
35	20.11212402855504	27.049081453064126	28.605865253416123	24.2329292649647
36	19.75301073780379	26.996974430975502	29.258537839387767	23.99147699183294
37	19.599655915679566	27.430837074096782	30.406869821432103	22.562637188791552
38	19.526191935770925	27.43044157487789	29.72176629951156	23.321600189839625
39	21.424192687219442	25.920425557159525	29.563665486760666	23.091716268860367
40	20.917657062627303	26.170479938302122	31.044513437085964	21.867349561984614
41	18.394668670529377	26.75690640510985	29.415847653700883	25.43257727065989
42	20.105697166248095	26.521782119480314	29.860289900927445	23.512230813344143
43	20.9105380766873	24.74806699756768	29.950265973224706	24.39112895252032
44	20.92368842571536	24.810061500128537	29.50176985900453	24.764480215151575
45	20.424469536672664	24.334177065000297	29.556348751211214	25.68500464711582
46	22.032668235480234	25.934070280211195	28.99760722972572	23.035654254582845
47	19.55961161976705	25.075639225612527	31.76550851312069	23.599240641499733
48	19.573454092428165	24.605291779548736	30.61114516798829	25.210108960034805
49	19.960845577329987	23.530028278194152	32.33255551820285	24.176570626273016
50	19.516007830884536	22.731614230061894	32.80230971543831	24.950068223615258
51	19.170044889161346	23.1220708339101	30.483201170677688	27.224683106250865
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	400.0
1	828.0
2	1256.0
3	12420.0
4	23584.0
5	15567.5
6	7551.0
7	7277.5
8	7004.0
9	7019.0
10	7034.0
11	6864.5
12	6695.0
13	6651.5
14	6608.0
15	6382.5
16	6157.0
17	5876.5
18	5596.0
19	5438.0
20	5280.0
21	5239.5
22	5199.0
23	5274.5
24	5350.0
25	5813.5
26	7112.0
27	7947.0
28	9492.0
29	11037.0
30	12473.0
31	13909.0
32	15882.0
33	17855.0
34	20562.0
35	23269.0
36	25495.0
37	27721.0
38	30538.5
39	33356.0
40	37191.5
41	41027.0
42	46931.0
43	52835.0
44	59874.5
45	66914.0
46	90212.5
47	113511.0
48	110667.0
49	107823.0
50	103229.5
51	98636.0
52	84698.5
53	70761.0
54	61917.0
55	53073.0
56	47725.5
57	42378.0
58	38796.5
59	35215.0
60	32199.0
61	29183.0
62	26393.0
63	23603.0
64	20140.0
65	16677.0
66	13907.0
67	11137.0
68	9469.0
69	7801.0
70	6477.0
71	5153.0
72	3941.0
73	2729.0
74	2193.0
75	1459.0
76	1261.0
77	893.0
78	525.0
79	368.0
80	211.0
81	176.5
82	142.0
83	72.5
84	3.0
85	16.0
86	29.0
87	16.5
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1011380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.837593705324615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85151030077357	27.941871183951417
2	9.000885002539874	6.631418894238271
3	3.468509756002818	3.8331465946375918
4	2.006626822923905	2.956772144843087
5	1.3270792256290636	2.444320266425013
6	0.9845220826546536	2.1760454684851287
7	0.7833342257360723	2.0199303560198323
8	0.6846553889518272	2.0176845637094725
9	0.5617250461531643	1.8623339121865485
>10	5.198784204304195	36.80930733324846
>50	0.08925254081392364	2.159556540238814
>100	0.034876791380725164	2.2735495971679742
>500	0.0035700652590503596	0.891655519627575
>1k	0.00411930606813503	3.6064805167600467
>5k	2.7462040454233534E-4	0.8131526057143689
>10k+	2.7462040454233534E-4	1.5627745027464008
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15448	1.527417983349483	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	8038	0.7947556803575314	Illumina PCR Primer Index 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	4073	0.40271707963376774	Illumina PCR Primer Index 2 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	3845	0.3801736241570923	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	3498	0.3458640669184678	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT	3426	0.33874508097846506	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	3270	0.3233206114417924	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	2873	0.2840673139670549	Illumina PCR Primer Index 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2083	0.20595621823646898	Illumina PCR Primer Index 2 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	2049	0.20259447487591212	Illumina PCR Primer Index 2 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	1996	0.1973541102256323	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	1857	0.1836105123692381	Illumina PCR Primer Index 2 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT	1759	0.17392078150645654	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	1660	0.16413217583895273	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCC	1243	0.12290138226977003	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	1013	0.10016017718365007	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.943740236113033E-4	0.0	0.0	0.19191599596590797	0.0
2	4.943740236113033E-4	0.0	0.0	0.9806403132353814	0.0
3	4.943740236113033E-4	0.0	0.0	1.4947892977911368	0.0
4	4.943740236113033E-4	0.0	0.0	2.37971880005537	0.0
5	4.943740236113033E-4	0.0	0.0	4.493662125017303	0.0
6	4.943740236113033E-4	0.0	0.0	6.177302299827958	0.0
7	4.943740236113033E-4	0.0	0.0	7.400087009828155	0.0
8	4.943740236113033E-4	0.0	0.0	9.089165298898534	0.0
9	4.943740236113033E-4	0.0	0.0	9.911012675749966	0.0
10	5.932488283335641E-4	0.0	0.0	11.515553006782811	0.0
11	5.932488283335641E-4	0.0	0.0	12.843837133421662	0.0
12	5.932488283335641E-4	0.0	0.0	14.289584528070558	0.0
13	5.932488283335641E-4	0.0	0.0	14.922778777511914	0.0
14	5.932488283335641E-4	0.0	0.0	15.207933714330915	0.0
15	5.932488283335641E-4	0.0	0.0	15.586228717198283	0.0
16	5.932488283335641E-4	0.0	0.0	16.186794281081294	0.0
17	5.932488283335641E-4	0.0	0.0	16.90294449168463	0.0
18	5.932488283335641E-4	0.0	0.0	17.764045166010796	0.0
19	5.932488283335641E-4	0.0	0.0	18.337024659376297	9.887480472226067E-5
20	5.932488283335641E-4	0.0	0.0	18.832881805058435	9.887480472226067E-5
21	5.932488283335641E-4	0.0	0.0	19.38460321540865	9.887480472226067E-5
22	5.932488283335641E-4	0.0	0.0	19.970831932606934	9.887480472226067E-5
23	5.932488283335641E-4	0.0	0.0	20.565563883011333	9.887480472226067E-5
24	5.932488283335641E-4	0.0	0.0	21.03709782673179	9.887480472226067E-5
25	5.932488283335641E-4	0.0	0.0	21.489153433921967	9.887480472226067E-5
26	5.932488283335641E-4	0.0	0.0	21.919357709268525	9.887480472226067E-5
27	5.932488283335641E-4	0.0	0.0	22.367754948683977	9.887480472226067E-5
28	5.932488283335641E-4	0.0	0.0	22.800628843758034	9.887480472226067E-5
29	5.932488283335641E-4	0.0	0.0	23.250805829658486	9.887480472226067E-5
30	5.932488283335641E-4	0.0	0.0	23.76396606616702	9.887480472226067E-5
31	5.932488283335641E-4	0.0	0.0	24.239455002076372	9.887480472226067E-5
32	5.932488283335641E-4	0.0	0.0	24.708912574897663	9.887480472226067E-5
33	5.932488283335641E-4	0.0	0.0	25.198342858272856	9.887480472226067E-5
34	5.932488283335641E-4	0.0	0.0	25.66750380667998	9.887480472226067E-5
35	5.932488283335641E-4	0.0	0.0	26.130435642389607	9.887480472226067E-5
36	6.921236330558247E-4	0.0	0.0	26.567363404457275	9.887480472226067E-5
37	6.921236330558247E-4	0.0	0.0	27.023077379422176	9.887480472226067E-5
38	6.921236330558247E-4	0.0	0.0	27.470980244814015	9.887480472226067E-5
39	6.921236330558247E-4	0.0	0.0	27.933714330914196	9.887480472226067E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	45	3.8380676E-10	45.0	24
GAGTACG	40	6.8139343E-9	45.0	1
AAATGCG	20	7.032884E-4	45.0	1
CGTTTTT	7690	0.0	43.566322	1
CGTTTCT	390	0.0	42.115383	1
GCTACGA	240	0.0	40.312496	10
TAGTGCG	40	3.4576806E-7	39.375	1
GTACGAG	40	3.4576806E-7	39.375	1
TCTAGCG	75	0.0	39.0	1
CGAATAT	245	0.0	38.57143	14
TCGCACA	35	6.2475447E-6	38.571426	37
TATCGCG	35	6.2475447E-6	38.571426	1
CGTTATT	310	0.0	38.46774	1
CGTATTC	30	1.1398251E-4	37.499996	17
TTGGACG	30	1.1398251E-4	37.499996	1
TTGTCGA	30	1.1398251E-4	37.499996	13
GTATACG	30	1.1398251E-4	37.499996	1
ACGTACG	30	1.1398251E-4	37.499996	1
TACGAAT	260	0.0	37.211536	12
GTTTTTT	9215	0.0	36.893654	2
>>END_MODULE