##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551241_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800831
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.564908201605583	31.0	31.0	34.0	30.0	34.0
2	31.712004155683285	31.0	31.0	34.0	30.0	34.0
3	31.698390796560073	33.0	31.0	34.0	30.0	34.0
4	35.437430618944575	37.0	35.0	37.0	33.0	37.0
5	34.207721729053944	37.0	35.0	37.0	32.0	37.0
6	34.69851566685106	37.0	35.0	37.0	32.0	37.0
7	35.454381760945815	37.0	35.0	37.0	33.0	37.0
8	35.23906791819997	37.0	35.0	37.0	32.0	37.0
9	37.13747095204856	39.0	37.0	39.0	34.0	39.0
10	36.69301762793898	39.0	35.0	39.0	32.0	39.0
11	36.82857431842673	39.0	37.0	39.0	33.0	39.0
12	36.831456324742675	39.0	37.0	39.0	33.0	39.0
13	36.91610214889284	39.0	37.0	39.0	33.0	39.0
14	37.923034947448336	40.0	37.0	41.0	33.0	41.0
15	38.04208503417075	40.0	37.0	41.0	33.0	41.0
16	38.14660396513122	40.0	37.0	41.0	34.0	41.0
17	38.036194902544985	40.0	37.0	41.0	33.0	41.0
18	37.94701129202041	39.0	37.0	41.0	33.0	41.0
19	37.84748592399645	39.0	37.0	41.0	33.0	41.0
20	37.860303859366084	40.0	36.0	41.0	34.0	41.0
21	37.763187239255224	39.0	36.0	41.0	33.0	41.0
22	37.88079007930512	40.0	36.0	41.0	33.0	41.0
23	37.90562678018208	40.0	36.0	41.0	34.0	41.0
24	37.81337760401383	40.0	36.0	41.0	33.0	41.0
25	37.543230719090545	39.0	35.0	41.0	33.0	41.0
26	37.64842769573106	39.0	36.0	41.0	33.0	41.0
27	37.67413224513037	40.0	36.0	41.0	33.0	41.0
28	37.55646572123207	40.0	36.0	41.0	33.0	41.0
29	37.5867729895571	40.0	36.0	41.0	33.0	41.0
30	37.30822483145632	39.0	35.0	41.0	33.0	41.0
31	37.18248794065165	39.0	35.0	41.0	32.0	41.0
32	37.12373522004018	39.0	35.0	41.0	32.0	41.0
33	37.04793021249177	40.0	35.0	41.0	32.0	41.0
34	36.836353737555115	40.0	35.0	41.0	31.0	41.0
35	36.790913438665584	40.0	35.0	41.0	31.0	41.0
36	36.595607562644304	40.0	35.0	41.0	30.0	41.0
37	36.57220187530203	40.0	35.0	41.0	31.0	41.0
38	36.41000285952966	39.0	35.0	41.0	30.0	41.0
39	36.34008673490412	39.0	35.0	41.0	30.0	41.0
40	36.246647544862775	39.0	35.0	41.0	29.0	41.0
41	36.03453537637779	39.0	35.0	41.0	28.0	41.0
42	36.19068442655192	39.0	35.0	41.0	29.0	41.0
43	36.09946543028429	39.0	35.0	41.0	29.0	41.0
44	36.20056416397467	39.0	35.0	41.0	30.0	41.0
45	36.146316763461954	39.0	35.0	41.0	29.0	41.0
46	36.05448964887723	39.0	35.0	41.0	29.0	41.0
47	35.91674897700014	39.0	35.0	41.0	29.0	41.0
48	35.887669183635495	39.0	35.0	41.0	29.0	41.0
49	35.83768984966866	39.0	35.0	40.0	29.0	41.0
50	35.67938054346048	38.0	35.0	40.0	28.0	41.0
51	34.76179118940201	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	18.0
9	37.0
10	30.0
11	50.0
12	41.0
13	34.0
14	45.0
15	56.0
16	97.0
17	161.0
18	239.0
19	526.0
20	1076.0
21	1905.0
22	2879.0
23	3997.0
24	5219.0
25	7074.0
26	9168.0
27	10400.0
28	10956.0
29	11162.0
30	12628.0
31	15648.0
32	20115.0
33	28264.0
34	47123.0
35	60022.0
36	64627.0
37	98773.0
38	168922.0
39	219475.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31958802793598	19.39048313564285	22.388244211325485	13.901684625095681
2	34.302118674227145	26.86496901343729	22.88235595275408	15.950556359581485
3	30.854949421288637	26.203655952379467	27.294023333262572	15.647371293069323
4	27.416645959010076	23.878446263943328	31.089081216885962	17.615826560160635
5	26.768694019087675	30.16379236068534	25.170229424185624	17.897284196041362
6	24.319737872285163	35.96763861538827	25.745381984463638	13.967241527862933
7	73.80908081730104	6.076937581087645	15.760878387574905	4.35310321403642
8	74.76571211653894	8.614801375071645	11.454102051494012	5.1653844568954
9	68.53343589346566	7.921396649230612	13.727615439462259	9.817552017841468
10	36.68239616098777	29.773073220192526	20.066031409872995	13.478499208946706
11	28.600790928423102	23.956115584936146	30.076133416413704	17.36696007022705
12	25.279615799088695	21.518397764322312	32.44791972338733	20.75406671320166
13	24.243442124493182	23.35861124257178	34.83931066604565	17.558635966889394
14	19.338412224302008	29.081666419007256	31.624649894921653	19.955271461769087
15	17.477095666876032	26.024342214524665	37.412013271214526	19.08654884738478
16	20.32913311297889	24.614431759010326	32.88059528165119	22.175839846359594
17	20.739456889156386	25.06633734208591	31.856908636154195	22.33729713260351
18	21.18474434680975	24.339966859424774	32.707400188054656	21.767888605710816
19	21.81471496483028	25.825298970694195	30.473345812037746	21.88664025243778
20	24.484941267258638	25.080697425549207	32.457659606084185	17.976701701107974
21	23.018714310509957	27.45473139776058	30.812368652062673	18.7141856396668
22	20.631818698327113	24.090975499200205	32.07380833159556	23.20339747087713
23	20.545783067838283	27.231837928351926	30.779527765533555	21.44285123827624
24	21.147408129805164	25.61264486514633	31.24704213498229	21.99290487006622
25	19.763345824524777	28.72828349551903	29.625726276829944	21.882644403126253
26	19.569547132915684	26.61235641477415	31.954557203704653	21.86353924860551
27	22.76660119301076	26.194540421137543	29.822147244549726	21.21671114130197
28	18.431479300876212	27.818853166273534	31.974161839389335	21.775505693460918
29	22.352531308103707	24.897512708673865	31.044627393295215	21.705328589927213
30	23.376967175346607	26.173562212252023	29.74272973948311	20.706740872918257
31	22.577672442750092	26.705135040976185	26.67004648920933	24.047146027064386
32	23.939632706526094	28.421227449986326	26.68253351830786	20.95660632517972
33	23.8110163068113	25.718659742192795	27.720455376977167	22.749868574018738
34	22.322437567976262	26.283572938610018	28.509885356585844	22.884104136827872
35	22.82928607908535	25.45468394705	27.76490920056791	23.95112077329674
36	23.694512325322073	26.48523845855118	27.97556538146001	21.84468383466674
37	22.40098098100598	28.159624190372252	28.141018517015446	21.29837631160632
38	21.123058423063043	27.321994278443267	26.4842394962233	25.07070780227039
39	22.295340714832466	27.78251591159683	28.313838999739023	21.608304373831682
40	22.813427552130225	24.896388876055	29.378108489806216	22.912075082008563
41	20.153315743271676	26.519452918281132	28.97665050428867	24.350580834158517
42	21.5286371281831	25.01276798725324	29.661938661215665	23.796656223348
43	22.82766276530254	24.984921912363532	29.28932571291571	22.898089609418218
44	22.158857486785603	25.69630796010644	28.940937601066892	23.203896952041067
45	21.28963539123735	24.58209035364515	28.425597910170808	25.702676344946685
46	23.297175059407042	26.91941246030686	27.600954508504287	22.182457971781812
47	20.304408795363816	26.077786699066348	32.02211203112766	21.595692474442174
48	20.999436834987655	25.545589518887258	29.241625261759346	24.21334838436574
49	20.955732233142825	23.92714567742757	31.924962944741146	23.192159144688453
50	21.33271564162726	24.083108670868135	30.320005094707874	24.264170592796734
51	20.249590737621297	24.032785943601084	28.438834161015247	27.278789157762372
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	305.0
1	676.0
2	1047.0
3	6700.5
4	12354.0
5	8289.0
6	4224.0
7	4120.0
8	4016.0
9	3930.0
10	3844.0
11	3657.0
12	3470.0
13	3469.5
14	3469.0
15	3238.5
16	3008.0
17	3094.5
18	3181.0
19	3043.0
20	2905.0
21	3028.5
22	3152.0
23	3651.5
24	4151.0
25	4974.5
26	6962.0
27	8126.0
28	10142.5
29	12159.0
30	13344.0
31	14529.0
32	15932.0
33	17335.0
34	19756.5
35	22178.0
36	23765.0
37	25352.0
38	27584.0
39	29816.0
40	32492.0
41	35168.0
42	38438.5
43	41709.0
44	45007.5
45	48306.0
46	50887.0
47	53468.0
48	71140.5
49	88813.0
50	81764.0
51	74715.0
52	72812.0
53	70909.0
54	60127.0
55	49345.0
56	43191.5
57	37038.0
58	33584.5
59	30131.0
60	28222.0
61	26313.0
62	23522.0
63	20731.0
64	17765.5
65	14800.0
66	12148.5
67	9497.0
68	7689.5
69	5882.0
70	4749.5
71	3617.0
72	3039.0
73	2461.0
74	1966.0
75	1243.5
76	1016.0
77	762.5
78	509.0
79	406.0
80	303.0
81	230.0
82	157.0
83	94.5
84	32.0
85	17.5
86	3.0
87	9.5
88	16.0
89	9.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	800831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.74155286089416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.98561910607711	22.301887544502673
2	9.868474384274924	5.870075051125852
3	3.422051400057823	3.053313678225499
4	1.6248960463054334	1.9330772661860391
5	0.9813617689016387	1.4593611462724343
6	0.6746639887496285	1.2039332810843266
7	0.5345206686197859	1.1128232304697097
8	0.4418812359311377	1.051378730934654
9	0.37788543727219254	1.0115009737193716
>10	6.811375682065511	48.7123909435576
>50	0.21703128374393746	3.9779903044169687
>100	0.05254891314971571	2.7595743244297837
>500	0.002563361617059303	0.5475184530789179
>1k	0.003417815489412404	1.695795143836444
>5k	0.001708907744706202	3.3093799281597622
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7465	0.9321567222048098	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC	6468	0.8076610420925263	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG	6372	0.7956734941579434	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	5740	0.7167554702552723	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	4087	0.5103448792566722	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	1729	0.21590073311347838	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCAGACTT	1509	0.18842926909672578	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCT	1390	0.1735697044694823	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT	1258	0.15708682605943075	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTC	1143	0.14272674259612828	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTA	1135	0.14172778026824637	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1095	0.1367329686288368	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	854	0.10663922850139416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22601522668328275	0.0
2	0.0	0.0	0.0	1.0843736069158163	0.0
3	0.0	0.0	0.0	1.3859353596451687	0.0
4	0.0	0.0	0.0	1.8986527744305604	0.0
5	0.0	0.0	0.0	3.5116023230868936	0.0
6	0.0	0.0	0.0	4.210751082313247	0.0
7	0.0	0.0	0.0	4.922886351802066	0.0
8	0.0	0.0	0.0	5.994398318746402	0.0
9	0.0	0.0	0.0	6.383743886038378	0.0
10	0.0	0.0	0.0	8.026412563949197	0.0
11	0.0	0.0	0.0	9.176343073632264	0.0
12	0.0	0.0	0.0	10.985713590008379	0.0
13	0.0	0.0	0.0	11.460845047207213	0.0
14	0.0	0.0	0.0	11.670127654898474	0.0
15	0.0	0.0	0.0	12.131398509797947	0.0
16	0.0	0.0	0.0	12.767987253240696	0.0
17	0.0	0.0	0.0	13.529696028250655	0.0
18	0.0	0.0	0.0	14.368699513380475	0.0
19	0.0	0.0	0.0	15.056609946418158	0.0
20	0.0	0.0	0.0	15.56620560392892	0.0
21	0.0	0.0	0.0	16.115385143682	0.0
22	0.0	0.0	0.0	16.716385854193955	0.0
23	0.0	0.0	0.0	17.26369233958226	0.0
24	0.0	0.0	0.0	17.71322538712912	0.0
25	0.0	0.0	0.0	18.11106213420809	0.0
26	0.0	0.0	0.0	18.509148621869034	0.0
27	0.0	0.0	0.0	18.914602456698105	0.0
28	0.0	0.0	0.0	19.325425714039543	0.0
29	0.0	0.0	0.0	19.73749767429083	0.0
30	0.0	0.0	0.0	20.20101619442804	0.0
31	0.0	0.0	0.0	20.641183970151005	0.0
32	0.0	0.0	0.0	21.071861603759096	0.0
33	0.0	0.0	0.0	21.481061547317722	0.0
34	0.0	0.0	0.0	21.91261327296271	0.0
35	0.0	0.0	0.0	22.347037015300355	0.0
36	0.0	0.0	0.0	22.770222431449334	0.0
37	0.0	0.0	0.0	23.170931195220962	0.0
38	0.0	0.0	0.0	23.564647222697424	0.0
39	0.0	0.0	0.0	23.985335233026692	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	65	0.0	45.000004	1
GTTAACG	35	1.2113196E-7	45.000004	1
GTCACGC	20	7.032003E-4	45.000004	37
ACTACCG	20	7.032003E-4	45.000004	10
CGCACGG	35	1.2113196E-7	45.000004	2
ATACGGA	20	7.032003E-4	45.000004	28
TACACGT	20	7.032003E-4	45.000004	41
GATTACG	25	3.8897422E-5	45.0	1
CGTTTTT	3730	0.0	42.345844	1
CGATGAA	835	0.0	40.958084	19
CTGCGCG	50	1.0804797E-9	40.5	1
TTGTCGG	45	1.9270374E-8	40.0	2
ATAACGG	85	0.0	39.705883	2
CGGGTCA	40	3.4564619E-7	39.375004	6
CGAATAT	160	0.0	39.375004	14
TATAGCG	40	3.4564619E-7	39.375004	1
TACGGCT	970	0.0	39.20103	7
AATGACG	75	0.0	39.000004	1
CTTGCGG	150	0.0	39.000004	2
CCGATGA	855	0.0	38.68421	18
>>END_MODULE