FastQCFastQC Report
Sat 18 Jun 2016
SRR3551239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858858
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95231.108797961944815No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC67590.7869752624997381No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG57090.6647198954891262No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC52560.6119754371502624No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC39910.46468682832319197No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT20880.24311352982681655No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT20460.23822331514639206No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTACAGT15070.17546556008094472No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC14630.17034247803478572No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT13460.15671973713931756No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCC12900.15019945089875159No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC11500.1338987352973367No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC10220.11899522389032878No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTA10060.1171322849644528No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT9170.1067696871892676No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTT207.0322887E-445.00000441
AACGGAT207.0322887E-445.0000044
ACGTAAG351.211447E-745.0000041
ACGATAG253.8899794E-545.01
ACGTCGG253.8899794E-545.02
TGTAGCG453.8380676E-1045.01
ACGGCCA302.1648593E-644.99999630
GGTGCGA302.1648593E-644.99999642
CGTTTTT45050.043.2519451
CGTAAGG900.042.52
ACGACCA900.042.528
TTTAGCG403.456862E-739.3750041
CGGCGAT1200.039.37499631
CACGACC1050.038.5714327
CGGGACG700.038.571436
CGAGACA1050.038.5714322
AACACGT1000.038.2541
GCGATCG301.13968184E-437.4999969
GTATACG301.13968184E-437.4999961
CGTCGCA301.13968184E-437.4999969