##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	858858
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623880781223438	33.0	31.0	34.0	30.0	34.0
2	31.775452985243195	33.0	31.0	34.0	30.0	34.0
3	31.74304832696441	33.0	31.0	34.0	30.0	34.0
4	35.47141785952975	37.0	35.0	37.0	33.0	37.0
5	34.25022297050269	37.0	35.0	37.0	32.0	37.0
6	34.75874358741492	37.0	35.0	37.0	32.0	37.0
7	35.49889388001276	37.0	35.0	37.0	33.0	37.0
8	35.33916083916084	37.0	35.0	37.0	33.0	37.0
9	37.240511237014736	39.0	37.0	39.0	34.0	39.0
10	36.743956509690776	39.0	35.0	39.0	32.0	39.0
11	36.8945122476591	39.0	37.0	39.0	33.0	39.0
12	36.91838697433103	39.0	37.0	39.0	33.0	39.0
13	37.027854429952335	39.0	37.0	39.0	33.0	39.0
14	38.078914092900106	40.0	37.0	41.0	33.0	41.0
15	38.17809812565057	40.0	37.0	41.0	33.0	41.0
16	38.248316951114155	40.0	37.0	41.0	34.0	41.0
17	38.15329658686302	40.0	37.0	41.0	34.0	41.0
18	38.018674798395075	39.0	37.0	41.0	33.0	41.0
19	37.91426289328387	39.0	37.0	41.0	34.0	41.0
20	37.94264709649325	40.0	36.0	41.0	34.0	41.0
21	37.84445740739447	39.0	36.0	41.0	33.0	41.0
22	37.97599370326643	40.0	36.0	41.0	34.0	41.0
23	38.031555856730684	40.0	36.0	41.0	34.0	41.0
24	37.91097131306922	40.0	36.0	41.0	34.0	41.0
25	37.63009018953075	39.0	35.0	41.0	33.0	41.0
26	37.77225571631166	40.0	36.0	41.0	33.0	41.0
27	37.83574467490551	40.0	36.0	41.0	34.0	41.0
28	37.726852401677576	40.0	36.0	41.0	33.0	41.0
29	37.7459475256678	40.0	36.0	41.0	33.0	41.0
30	37.459938662735865	40.0	36.0	41.0	33.0	41.0
31	37.34757783009531	39.0	35.0	41.0	33.0	41.0
32	37.27036716197556	40.0	36.0	41.0	33.0	41.0
33	37.158316042931425	40.0	36.0	41.0	32.0	41.0
34	36.956528320164686	40.0	36.0	41.0	31.0	41.0
35	36.87034760111683	40.0	36.0	41.0	31.0	41.0
36	36.689095286997386	40.0	35.0	41.0	31.0	41.0
37	36.67774300291783	40.0	35.0	41.0	31.0	41.0
38	36.47130026151005	39.0	35.0	41.0	30.0	41.0
39	36.42082509565027	39.0	35.0	41.0	30.0	41.0
40	36.33602760875488	39.0	35.0	41.0	30.0	41.0
41	36.22637618791465	39.0	35.0	41.0	30.0	41.0
42	36.324289929185035	39.0	35.0	41.0	30.0	41.0
43	36.268919891297514	39.0	35.0	41.0	30.0	41.0
44	36.35897785198485	39.0	35.0	41.0	30.0	41.0
45	36.35304555584276	39.0	35.0	41.0	30.0	41.0
46	36.28333787424697	39.0	35.0	41.0	30.0	41.0
47	36.23198479841837	39.0	35.0	41.0	30.0	41.0
48	36.18254472799927	39.0	35.0	41.0	30.0	41.0
49	36.17886193061018	39.0	35.0	41.0	30.0	41.0
50	36.05059509255314	39.0	35.0	41.0	29.0	41.0
51	35.15195876384688	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	19.0
9	28.0
10	37.0
11	27.0
12	40.0
13	35.0
14	38.0
15	83.0
16	96.0
17	157.0
18	264.0
19	517.0
20	1004.0
21	1765.0
22	2635.0
23	3718.0
24	5137.0
25	7994.0
26	10429.0
27	11285.0
28	10998.0
29	11067.0
30	12318.0
31	15330.0
32	20155.0
33	28152.0
34	48698.0
35	61537.0
36	67714.0
37	102616.0
38	181514.0
39	253382.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21640830032438	19.64387593758223	23.644187979852315	14.49552778224107
2	34.8852778922709	25.92326088829585	23.363582804142244	15.827878415291002
3	29.462379112728765	25.73067957683342	29.5237396635998	15.28320164683801
4	26.35092180546726	24.310188645852982	32.42445200487158	16.914437543808173
5	25.25283574234623	30.5287952141099	26.506127904729304	17.712241138814566
6	24.222048347922474	34.63203463203463	27.071180567684067	14.07473645235883
7	73.93410785019177	5.675327003998333	15.812392735469658	4.578172410340243
8	74.46877132191817	7.24671598797473	12.851018445424039	5.433494244683056
9	68.53053706200559	7.43673575841408	14.918880653146388	9.113846526433939
10	35.688670304054924	29.54865647173339	20.952008364595777	13.81066485961591
11	26.956144089011218	23.961004030934102	30.95086731450368	18.131984565550997
12	24.17955005367593	21.037936422551805	34.128691820999514	20.653821702772753
13	23.26088829585333	23.631147407371184	35.57572963167369	17.532234665101797
14	19.049481986544926	28.325287765847207	32.15630523322831	20.46892501437956
15	17.588705001292414	25.287882280889274	37.34854888701042	19.774863830807888
16	20.49139671517294	25.210919616514023	32.865968530304194	21.431715138008844
17	20.734975979731225	25.317107135288953	31.331489023796717	22.616427861183105
18	21.080784017846955	24.4807639912535	32.76758206828137	21.670869922618174
19	21.423215479159534	26.387132680838977	30.3684660328017	21.821185807199793
20	22.52269874647497	25.85852376062166	32.25119868476512	19.36757880813825
21	21.92981843331494	28.488411355544223	30.869130869130867	18.71263934200997
22	20.37449729757422	23.961004030934102	31.886877691073494	23.77762098041818
23	19.80956106830233	26.984204606582228	31.201432600033996	22.004801725081446
24	20.221969173018124	25.628916538007445	31.989805066728145	22.159309222246286
25	19.349997322025295	28.68797868797869	29.97550235312473	21.986521636871288
26	18.593993419168246	26.547811163195778	32.968779472275976	21.889415945360003
27	21.123398745776367	26.227036366896506	31.449785645589838	21.199779241737286
28	19.183613589207997	25.638347666319696	33.72757778352184	21.450460960950473
29	20.002258813447625	23.626839361105095	34.19936706649994	22.17153475894735
30	20.642178334486026	26.371879868383363	32.7742187882048	20.211723008925805
31	22.513500485528457	25.150490535105916	29.78548258268538	22.550526396680244
32	22.255017709563162	26.682175633224585	30.974037617394263	20.08876903981799
33	21.490630581539673	26.316224567972817	29.679411497593318	22.513733352894192
34	20.521669472718422	25.266342049558833	30.998837991844987	23.213150485877758
35	19.980252847385714	26.28490390728153	31.461196146510833	22.273647098821925
36	23.205232995442785	26.896064308651724	30.048972007013962	19.84973068889153
37	20.99206155150211	26.256610522344786	32.18226994450771	20.569057981645393
38	21.03537488152873	28.65910313462761	28.393052169275947	21.912469814567718
39	21.891395317968744	25.790875790875788	29.852548384016913	22.465180507138548
40	20.814500185129557	27.053482647888245	30.455907728635	21.6761094383472
41	20.719257432544147	25.66815468913371	29.028896511413993	24.583691366908152
42	21.50681486345822	26.09057609057609	30.621942160403698	21.780666885561992
43	23.159940292807427	24.621183012791402	30.27392188231349	21.94495481208768
44	21.47048755440364	24.44781325900207	30.508768620656735	23.572930565937558
45	20.383695558520735	24.66880438908411	30.173788915047655	24.7737111373475
46	22.895752266381635	25.980895561315144	29.21868341448761	21.90466875781561
47	19.41578235284529	24.846715056505268	33.154258329083504	22.58324426156594
48	20.67128675520284	25.128251701678273	30.589922897615207	23.61053864550368
49	21.25706461370797	23.28976384920441	32.38614532320826	23.06702621387936
50	20.308130098339888	23.204883694394184	31.94544383355572	24.541542373710207
51	20.211257274194335	22.774894103565433	29.864773920717973	27.149074701522252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	302.0
1	652.5
2	1003.0
3	7980.0
4	14957.0
5	9915.0
6	4873.0
7	4669.5
8	4466.0
9	4438.0
10	4410.0
11	4278.0
12	4146.0
13	4049.0
14	3952.0
15	3879.0
16	3806.0
17	3556.5
18	3307.0
19	3259.0
20	3211.0
21	3615.5
22	4020.0
23	4188.0
24	4356.0
25	4652.5
26	6230.0
27	7511.0
28	8561.5
29	9612.0
30	11540.5
31	13469.0
32	15234.5
33	17000.0
34	19309.0
35	21618.0
36	24890.0
37	28162.0
38	31144.5
39	34127.0
40	37925.0
41	41723.0
42	48532.5
43	55342.0
44	77299.5
45	99257.0
46	96049.0
47	92841.0
48	89059.0
49	85277.0
50	74517.0
51	63757.0
52	56398.0
53	49039.0
54	43662.0
55	38285.0
56	35121.5
57	31958.0
58	29842.0
59	27726.0
60	25966.5
61	24207.0
62	21232.0
63	18257.0
64	15924.0
65	13591.0
66	10857.0
67	8123.0
68	6842.0
69	5561.0
70	4746.0
71	3931.0
72	3447.5
73	2964.0
74	2423.5
75	1385.0
76	887.0
77	739.5
78	592.0
79	404.0
80	216.0
81	153.5
82	91.0
83	82.0
84	73.0
85	41.0
86	9.0
87	8.5
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	858858.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.11809919356024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.87887431309548	25.54724861316322
2	9.921464128369282	6.770029945541058
3	3.4986901862679805	3.581059764678802
4	1.7934476732430273	2.447561024566617
5	1.209687350925361	2.0636116516033294
6	0.8745474731581054	1.7902738463211423
7	0.6685878088744286	1.5967661627947993
8	0.5812482153308619	1.5864867413390569
9	0.4987600955202552	1.5315071771474684
>10	5.911439136109498	41.75735074923945
>50	0.10813358444370223	2.4652511622101856
>100	0.047396208148869814	2.940788852230752
>500	0.002808664186599645	0.6329326013273244
>1k	0.0035108302332495555	2.025408280818268
>5k	0.0014043320932998224	3.2637234270184723
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9523	1.108797961944815	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	6759	0.7869752624997381	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	5709	0.6647198954891262	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	5256	0.6119754371502624	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	3991	0.46468682832319197	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	2088	0.24311352982681655	No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	2046	0.23822331514639206	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTACAGT	1507	0.17546556008094472	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1463	0.17034247803478572	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT	1346	0.15671973713931756	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCC	1290	0.15019945089875159	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC	1150	0.1338987352973367	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1022	0.11899522389032878	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTA	1006	0.1171322849644528	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT	917	0.1067696871892676	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24590793821563053	0.0
2	0.0	0.0	0.0	1.1098458650906204	0.0
3	0.0	0.0	0.0	1.5224868371721518	0.0
4	0.0	0.0	0.0	2.264635131767999	0.0
5	0.0	0.0	0.0	4.222816810229397	0.0
6	0.0	0.0	0.0	5.317293429181541	0.0
7	0.0	0.0	0.0	6.37090182544728	0.0
8	0.0	0.0	0.0	7.919469807581696	0.0
9	0.0	0.0	0.0	8.599442515526432	0.0
10	0.0	0.0	0.0	10.515358767107019	0.0
11	0.0	0.0	0.0	11.993367937423882	0.0
12	0.0	0.0	0.0	13.86690232844079	0.0
13	0.0	0.0	0.0	14.470028805693142	0.0
14	0.0	0.0	0.0	14.747024537234328	0.0
15	0.0	0.0	0.0	15.239771883128526	0.0
16	0.0	0.0	0.0	15.95968134429673	0.0
17	0.0	0.0	0.0	16.820708429100037	0.0
18	0.0	0.0	0.0	17.843461899405956	0.0
19	0.0	0.0	0.0	18.62007456413051	0.0
20	0.0	0.0	0.0	19.244857706396168	0.0
21	0.0	0.0	0.0	19.922967475415028	0.0
22	0.0	0.0	0.0	20.644041273411904	0.0
23	0.0	0.0	0.0	21.340663998006654	0.0
24	0.0	0.0	0.0	21.90711386515582	0.0
25	0.0	0.0	0.0	22.411504579336746	0.0
26	0.0	0.0	0.0	22.905183394693886	0.0
27	0.0	0.0	0.0	23.422731115038808	0.0
28	0.0	0.0	0.0	23.91990294088196	0.0
29	0.0	0.0	0.0	24.444087381150318	0.0
30	0.0	0.0	0.0	25.04930966469428	0.0
31	0.0	0.0	0.0	25.57419270705984	0.0
32	0.0	0.0	0.0	26.095000570525045	0.0
33	0.0	0.0	0.0	26.59985701943744	0.0
34	0.0	0.0	0.0	27.088179885382683	0.0
35	0.0	0.0	0.0	27.58232443547129	0.0
36	0.0	0.0	0.0	28.077284021339967	0.0
37	0.0	0.0	0.0	28.55792226421597	0.0
38	0.0	0.0	0.0	29.055909125839197	0.0
39	0.0	0.0	0.0	29.572641810404047	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCTT	20	7.0322887E-4	45.000004	41
AACGGAT	20	7.0322887E-4	45.000004	4
ACGTAAG	35	1.211447E-7	45.000004	1
ACGATAG	25	3.8899794E-5	45.0	1
ACGTCGG	25	3.8899794E-5	45.0	2
TGTAGCG	45	3.8380676E-10	45.0	1
ACGGCCA	30	2.1648593E-6	44.999996	30
GGTGCGA	30	2.1648593E-6	44.999996	42
CGTTTTT	4505	0.0	43.251945	1
CGTAAGG	90	0.0	42.5	2
ACGACCA	90	0.0	42.5	28
TTTAGCG	40	3.456862E-7	39.375004	1
CGGCGAT	120	0.0	39.374996	31
CACGACC	105	0.0	38.57143	27
CGGGACG	70	0.0	38.57143	6
CGAGACA	105	0.0	38.57143	22
AACACGT	100	0.0	38.25	41
GCGATCG	30	1.13968184E-4	37.499996	9
GTATACG	30	1.13968184E-4	37.499996	1
CGTCGCA	30	1.13968184E-4	37.499996	9
>>END_MODULE