##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920781
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51462399854037	31.0	31.0	34.0	30.0	34.0
2	31.672107699876516	31.0	31.0	34.0	30.0	34.0
3	31.660248202341272	31.0	31.0	34.0	30.0	34.0
4	35.39669910651936	37.0	35.0	37.0	33.0	37.0
5	34.25670056180569	37.0	35.0	37.0	32.0	37.0
6	34.663889676263956	37.0	35.0	37.0	32.0	37.0
7	35.339888638014905	37.0	35.0	37.0	32.0	37.0
8	35.00725905508476	37.0	35.0	37.0	32.0	37.0
9	36.73466872144408	39.0	37.0	39.0	32.0	39.0
10	36.46091633081048	38.0	35.0	39.0	32.0	39.0
11	36.657118250702396	39.0	35.0	39.0	32.0	39.0
12	36.61256151028312	39.0	35.0	39.0	32.0	39.0
13	36.642620775189755	39.0	35.0	39.0	32.0	39.0
14	37.69567030596852	40.0	36.0	41.0	33.0	41.0
15	37.769352321561804	40.0	36.0	41.0	33.0	41.0
16	37.893292759081696	40.0	37.0	41.0	33.0	41.0
17	37.694895963318096	40.0	36.0	41.0	33.0	41.0
18	37.61606831591877	39.0	36.0	41.0	33.0	41.0
19	37.5275271753001	39.0	36.0	41.0	33.0	41.0
20	37.39120811571915	39.0	35.0	41.0	32.0	41.0
21	37.33125031902266	39.0	35.0	41.0	32.0	41.0
22	37.41376939793501	39.0	35.0	41.0	32.0	41.0
23	37.37861120070896	39.0	35.0	41.0	32.0	41.0
24	37.36243580178132	39.0	35.0	41.0	32.0	41.0
25	37.135092926548225	39.0	35.0	41.0	32.0	41.0
26	37.145479761202715	39.0	35.0	41.0	32.0	41.0
27	37.084456564590276	39.0	35.0	41.0	32.0	41.0
28	36.911200383152995	39.0	35.0	41.0	31.0	41.0
29	36.95728083007794	39.0	35.0	41.0	31.0	41.0
30	36.637187344221914	39.0	35.0	41.0	31.0	41.0
31	36.59516540849562	39.0	35.0	41.0	30.0	41.0
32	36.48739711179966	39.0	35.0	41.0	30.0	41.0
33	36.34104417880039	39.0	35.0	41.0	30.0	41.0
34	36.10741533545979	39.0	35.0	41.0	29.0	41.0
35	36.023800447663454	39.0	35.0	41.0	29.0	41.0
36	35.762838286194004	39.0	35.0	41.0	27.0	41.0
37	35.67734890272497	39.0	35.0	41.0	27.0	41.0
38	35.52687664059098	38.0	35.0	41.0	26.0	41.0
39	35.39752775089842	38.0	35.0	40.0	26.0	41.0
40	35.351848050730844	38.0	35.0	40.0	26.0	41.0
41	35.15669741230543	38.0	35.0	40.0	25.0	41.0
42	35.20043419662222	38.0	35.0	40.0	25.0	41.0
43	35.148116653145536	38.0	35.0	40.0	25.0	41.0
44	35.29848791406425	38.0	35.0	40.0	26.0	41.0
45	35.32547152906066	38.0	35.0	40.0	26.0	41.0
46	35.25093263218941	38.0	35.0	40.0	26.0	41.0
47	35.09585015329378	38.0	35.0	40.0	26.0	41.0
48	35.119198810574936	38.0	35.0	40.0	26.0	41.0
49	35.099242925299286	37.0	35.0	40.0	26.0	41.0
50	34.92643527614058	37.0	34.0	40.0	25.0	41.0
51	34.114676562613695	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	7.0
8	24.0
9	41.0
10	68.0
11	69.0
12	53.0
13	55.0
14	84.0
15	133.0
16	177.0
17	266.0
18	499.0
19	1217.0
20	2714.0
21	4366.0
22	5753.0
23	7411.0
24	8927.0
25	10939.0
26	12941.0
27	13921.0
28	13609.0
29	14089.0
30	16088.0
31	20383.0
32	26112.0
33	36606.0
34	61831.0
35	75207.0
36	78720.0
37	112037.0
38	176347.0
39	220029.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.00596558790853	17.892093776913295	20.57774867205123	14.524191963126952
2	32.27075710728175	30.81612240044049	21.242076020248028	15.671044472029722
3	29.054031306032595	29.988781262862723	25.96936730883891	14.987820122265772
4	26.197543172589356	22.14272449149146	34.04468597853344	17.61504635738574
5	29.44250587273195	28.790776525579915	23.682721515756732	18.0839960859314
6	24.077712289893036	37.4688443831921	24.928728981158386	13.524714345756482
7	70.45095413567395	6.1604225108902115	18.845740735310567	4.542882618125265
8	70.53837991878633	12.319433176835751	11.92541983381499	5.216767070562924
9	64.50578367711758	7.831721115009975	13.654821287580868	14.007673920291577
10	41.80755250162634	22.653920964919998	20.31992406446267	15.218602468990996
11	33.43954751455558	22.494708296543912	25.013113867466856	19.052630321433654
12	26.005749467028533	19.474989166805138	29.902550117780454	24.61671124838588
13	27.017825085443768	21.890330056767027	32.27933678040707	18.812508077382134
14	20.3116702017092	29.922533153920423	28.813257441237383	20.952539203132993
15	18.69087220522578	22.727228298585658	35.30915603167311	23.27274346451545
16	21.574837013361485	22.993958389671377	29.131574174532272	26.299630422434866
17	21.27650331620657	26.249890038999503	28.628631563857205	23.844975080936727
18	22.518383850231487	22.049542725143112	29.231054941403006	26.201018483222395
19	23.122870693465654	23.811633819551012	26.792798721954515	26.27269676502882
20	27.466683174392177	23.471487791342348	29.188265179233714	19.87356385503176
21	23.449549892971294	28.44259384153235	28.239288169499588	19.868568095996768
22	22.170092562726644	21.610350343892847	31.086327802159254	25.133229291221255
23	24.856616285522833	24.938394688856526	28.376237129132768	21.828751896487873
24	23.022086685107535	25.1880740371489	28.437489479040075	23.352349798703493
25	21.728076491587032	29.425889543767735	26.305277802213556	22.540756162431673
26	20.759333652627497	23.032512617006653	30.63616647172346	25.57198725864239
27	25.846971212481577	23.2872963277913	27.301497315865554	23.56423514386157
28	20.09913323580743	26.39856817201919	30.54689442983728	22.955404162336105
29	22.910659537935732	22.330499869132833	30.49976052937669	24.259080063554745
30	21.059187798184368	26.56646911697787	30.816556814269624	21.557786270568137
31	23.909051120733377	23.477026567663756	27.332557904648336	25.28136440695453
32	28.310314830562316	24.50767337727429	26.0245378651384	21.15747392702499
33	25.451003007229733	25.228691730172535	25.72598696106892	23.594318301528812
34	22.847995343083753	26.03485519358023	26.705264335384854	24.411885127951162
35	26.83634870832478	23.398289061133973	27.265658174962343	22.499704055578906
36	24.10529756804278	27.607650461944804	26.177125722620254	22.10992624739216
37	23.983010075142733	25.105644013071508	28.804677768112068	22.106668143673687
38	23.052169842774774	25.939066944257107	26.254234177290797	24.754529035677322
39	25.790280207780135	26.10599045810024	25.355323361363887	22.748405972755737
40	24.171328470070517	24.49160006559649	27.634366912436292	23.702704551896705
41	22.31714164388709	26.252713728888843	25.931899116076462	25.498245511147598
42	24.863675510246193	23.828141545057946	28.514706537167907	22.793476407527958
43	25.83502483218051	23.110924313164585	26.328518942072005	24.725531912582905
44	23.355933712793814	22.577789941364994	29.070864841911376	24.995411503929816
45	23.180973543111772	22.711589400736983	27.431712861147222	26.675724195004026
46	25.881724318811965	25.822969848422154	25.8876975089625	22.40760832380338
47	21.534110716880562	25.427653263914003	30.771486379497404	22.26674963970803
48	22.591148166610736	24.900492082264947	28.014913426754028	24.49344632437029
49	23.444445530479015	22.346355973896074	31.169735257352183	23.039463238272727
50	22.80672602931642	22.718865832374906	29.75246013981609	24.72194799849258
51	22.29683279737527	22.920542452548435	27.761650164371332	27.02097458570496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	385.0
1	995.0
2	1605.0
3	8469.0
4	15333.0
5	10101.0
6	4869.0
7	4527.0
8	4185.0
9	3970.5
10	3756.0
11	3711.0
12	3666.0
13	3649.0
14	3632.0
15	3312.0
16	2992.0
17	2900.5
18	2809.0
19	2768.0
20	2727.0
21	3347.5
22	3968.0
23	3736.0
24	3504.0
25	4302.5
26	5861.0
27	6621.0
28	7418.0
29	8215.0
30	9462.0
31	10709.0
32	12139.0
33	13569.0
34	15231.0
35	16893.0
36	18021.5
37	19150.0
38	21119.5
39	23089.0
40	26664.0
41	30239.0
42	33751.0
43	37263.0
44	43216.0
45	49169.0
46	79208.0
47	109247.0
48	92073.5
49	74900.0
50	73291.0
51	71682.0
52	66613.5
53	61545.0
54	58368.0
55	55191.0
56	53705.5
57	52220.0
58	49041.0
59	45862.0
60	44644.5
61	43427.0
62	39213.0
63	34999.0
64	31805.0
65	28611.0
66	25006.0
67	21401.0
68	18842.5
69	16284.0
70	13918.5
71	11553.0
72	9818.5
73	8084.0
74	6728.5
75	4405.0
76	3437.0
77	2716.0
78	1995.0
79	1429.0
80	863.0
81	629.0
82	395.0
83	284.5
84	174.0
85	120.0
86	66.0
87	41.0
88	16.0
89	9.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	920781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.069803823954725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.71075375903212	21.251860053797742
2	8.182035137212093	4.593362423644959
3	2.8629110377012355	2.41084053581125
4	1.5267482037094777	1.7142209026680122
5	1.0501544187165102	1.4738814259115824
6	0.801189792589639	1.3493544182247683
7	0.6809097020381921	1.3379101230627661
8	0.5790903055457803	1.3003961018419246
9	0.5288010523504105	1.3359007621239152
>10	7.809912052529763	48.27188397904306
>50	0.19027141337288223	3.3720178847661617
>100	0.06806780623470346	3.5237525165182157
>500	0.004378630810419521	0.8953238708456879
>1k	0.003184458771214197	2.2956850431043936
>5k	0.001194172039205324	3.197824682840832
>10k+	3.980573464017746E-4	1.675785275794787
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	14998	1.628834652322322	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC	9674	1.0506298457505097	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG	9629	1.045742690172799	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9317	1.0118584115006717	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT	4051	0.439952605451242	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	3880	0.42138141425594144	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT	3381	0.3671882890719943	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGAAGGT	2394	0.2599966767342071	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT	2247	0.2440319685136857	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTC	1898	0.20612936192210743	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTA	1615	0.17539458351117151	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1080	0.11729173386505587	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1720691456491827E-4	0.0	0.0	0.09676568043867108	0.0
2	2.1720691456491827E-4	0.0	0.0	0.7027729720747931	0.0
3	2.1720691456491827E-4	0.0	0.0	0.9173734036649323	0.0
4	2.1720691456491827E-4	0.0	0.0	1.5856104763239034	0.0
5	2.1720691456491827E-4	0.0	0.0	3.827511645005707	0.0
6	2.1720691456491827E-4	0.0	0.0	4.719471839666544	0.0
7	2.1720691456491827E-4	0.0	0.0	5.395854171621699	0.0
8	2.1720691456491827E-4	0.0	0.0	6.255776346384211	0.0
9	2.1720691456491827E-4	0.0	0.0	6.570834975960625	0.0
10	2.1720691456491827E-4	0.0	0.0	8.277864117526317	0.0
11	2.1720691456491827E-4	0.0	0.0	9.02082036879562	0.0
12	2.1720691456491827E-4	0.0	0.0	10.796486895363827	0.0
13	2.1720691456491827E-4	0.0	0.0	11.220474792594548	0.0
14	2.1720691456491827E-4	0.0	0.0	11.425626723401113	0.0
15	2.1720691456491827E-4	0.0	0.0	11.832563877838487	0.0
16	2.1720691456491827E-4	0.0	0.0	12.26991000031495	0.0
17	2.1720691456491827E-4	0.0	0.0	12.718333675434224	0.0
18	2.1720691456491827E-4	0.0	0.0	13.232462442209386	0.0
19	2.1720691456491827E-4	0.0	0.0	13.817292059675427	0.0
20	2.1720691456491827E-4	0.0	0.0	14.16145641580354	0.0
21	3.258103718473774E-4	0.0	0.0	14.503014288956875	0.0
22	3.258103718473774E-4	0.0	0.0	14.88171454450081	0.0
23	3.258103718473774E-4	0.0	0.0	15.206656088689927	0.0
24	4.3441382912983654E-4	0.0	0.0	15.50314352707104	0.0
25	4.3441382912983654E-4	0.0	0.0	15.773674739161647	0.0
26	4.3441382912983654E-4	0.0	0.0	16.106218525360536	0.0
27	4.3441382912983654E-4	0.0	0.0	16.43007403497683	0.0
28	5.430172864122956E-4	0.0	0.0	16.69191697048484	0.0
29	5.430172864122956E-4	0.0	0.0	16.95169644030448	0.0
30	5.430172864122956E-4	0.0	0.0	17.283154191930546	0.0
31	5.430172864122956E-4	0.0	0.0	17.570301732985367	0.0
32	5.430172864122956E-4	0.0	0.0	17.875477447949077	0.0
33	5.430172864122956E-4	0.0	0.0	18.184345680460392	0.0
34	5.430172864122956E-4	0.0	0.0	18.487457929735736	0.0
35	6.516207436947548E-4	0.0017376553165193462	0.0	18.80664349068888	0.0
36	6.516207436947548E-4	0.0017376553165193462	0.0	19.081084427241656	0.0
37	6.516207436947548E-4	0.0017376553165193462	0.0	19.363236209261487	0.0
38	6.516207436947548E-4	0.0017376553165193462	0.0	19.65266442291924	0.0
39	6.516207436947548E-4	0.0017376553165193462	0.0	19.956862706767406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAATCG	25	3.8901984E-5	45.000004	44
CGTATTA	20	7.0325565E-4	45.0	25
ATATGCG	35	1.2115743E-7	45.0	1
TACGATC	20	7.0325565E-4	45.0	11
CGGTCGA	220	0.0	42.954544	41
TACGGGT	90	0.0	42.500004	4
GATGAAT	2680	0.0	42.481342	20
GTCGAAT	350	0.0	41.785713	43
CGATGAA	2615	0.0	41.644356	19
CGTTTTT	4325	0.0	41.56647	1
CCGATGA	2585	0.0	41.257256	18
ATACGGA	60	3.6379788E-12	41.250004	28
ATGAATG	2720	0.0	41.19485	21
ATAGCGG	165	0.0	40.909092	2
TATAGCG	50	1.0804797E-9	40.500004	1
CGCACGG	50	1.0804797E-9	40.500004	2
TACGGGA	195	0.0	40.384617	4
GGTCGAA	370	0.0	40.135136	42
TCGAATG	385	0.0	39.740257	44
CGAATGC	375	0.0	39.600002	45
>>END_MODULE