FastQCFastQC Report
Sat 18 Jun 2016
SRR3551237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856345
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148381.7327128668936No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT19510.22782873724958982No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC17980.20996210639403512No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC15490.18088504049185783No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG13640.1592815979540956No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT11560.1349923220197467No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC11480.13405811909919485No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC9670.11292177802170855No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG9260.10813398805388015No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC9250.10801721268881116No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCACG207.0322776E-445.01
CGTTTTT67350.042.9955441
CGTAAGG1200.041.2499962
CGGTCTA700.038.5714331
AACGTAG659.094947E-1238.0769271
CGTTATT2850.037.8947371
TCGTTTA301.1396791E-437.49999638
TACGATA301.1396791E-437.49999643
GCGTAAG850.037.0588231
GTTTTTT79500.037.0188672
ATAACGG552.748493E-936.818182
GTACGAG552.748493E-936.818181
CACGACG800.036.562526
ACGGTCT751.8189894E-1236.00000430
AACGTAA250.002106809536.042
GATTTCG250.002106809536.020
TAGCACG250.002106809536.01
TACGCGG250.002106809536.02
CGTAGCG250.002106809536.02
TGCGACG250.002106809536.01