##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	856345
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.626230082501795	33.0	31.0	34.0	30.0	34.0
2	31.806930617916844	33.0	31.0	34.0	30.0	34.0
3	31.75642760803181	33.0	31.0	34.0	30.0	34.0
4	35.45830360427164	37.0	35.0	37.0	33.0	37.0
5	34.36210055526686	37.0	35.0	37.0	32.0	37.0
6	34.772398974712296	37.0	35.0	37.0	32.0	37.0
7	35.4859817013003	37.0	35.0	37.0	33.0	37.0
8	35.36332085783183	37.0	35.0	37.0	33.0	37.0
9	37.32002872673981	39.0	37.0	39.0	34.0	39.0
10	36.772108204053275	39.0	37.0	39.0	32.0	39.0
11	36.85310476501877	39.0	37.0	39.0	33.0	39.0
12	36.86420893448318	39.0	37.0	39.0	33.0	39.0
13	37.01669420619026	39.0	37.0	39.0	33.0	39.0
14	38.08942423906253	40.0	37.0	41.0	33.0	41.0
15	38.21669070292931	40.0	37.0	41.0	33.0	41.0
16	38.222724486042424	40.0	37.0	41.0	34.0	41.0
17	38.10913241742522	40.0	37.0	41.0	33.0	41.0
18	37.961763074461814	39.0	37.0	41.0	33.0	41.0
19	37.81068728141111	39.0	37.0	41.0	33.0	41.0
20	37.86815127080791	39.0	36.0	41.0	34.0	41.0
21	37.686351879207564	39.0	35.0	41.0	33.0	41.0
22	37.799551582598134	40.0	35.0	41.0	33.0	41.0
23	37.8693213599659	40.0	35.0	41.0	34.0	41.0
24	37.73053967734967	40.0	35.0	41.0	33.0	41.0
25	37.41355995539181	39.0	35.0	41.0	33.0	41.0
26	37.55153822349637	39.0	35.0	41.0	33.0	41.0
27	37.622725653796074	40.0	35.0	41.0	33.0	41.0
28	37.463522295336574	39.0	35.0	41.0	33.0	41.0
29	37.44868948846551	40.0	35.0	41.0	33.0	41.0
30	37.111034688122196	39.0	35.0	41.0	32.0	41.0
31	36.95627580005722	39.0	35.0	41.0	32.0	41.0
32	36.849956501176514	39.0	35.0	41.0	31.0	41.0
33	36.71195020698433	39.0	35.0	41.0	31.0	41.0
34	36.46419959245397	39.0	35.0	41.0	30.0	41.0
35	36.37798200491624	39.0	35.0	41.0	30.0	41.0
36	36.16109979038822	39.0	35.0	41.0	30.0	41.0
37	36.15947194179916	39.0	35.0	41.0	30.0	41.0
38	35.95026887527807	39.0	35.0	41.0	29.0	41.0
39	35.91598012483286	39.0	35.0	41.0	29.0	41.0
40	35.84072657632146	39.0	35.0	41.0	28.0	41.0
41	35.681753265331146	39.0	35.0	41.0	27.0	41.0
42	35.778496984276195	39.0	35.0	41.0	28.0	41.0
43	35.72027161949915	39.0	35.0	41.0	28.0	41.0
44	35.72953307370277	39.0	35.0	41.0	28.0	41.0
45	35.68870373506005	39.0	35.0	41.0	27.0	41.0
46	35.617956547886656	39.0	35.0	41.0	27.0	41.0
47	35.5703764253893	38.0	35.0	41.0	27.0	41.0
48	35.50438900209612	38.0	35.0	40.0	27.0	41.0
49	35.466125218223965	38.0	35.0	40.0	27.0	41.0
50	35.37860558536571	38.0	35.0	40.0	27.0	41.0
51	34.48745540640746	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	20.0
9	47.0
10	46.0
11	55.0
12	42.0
13	82.0
14	95.0
15	101.0
16	168.0
17	260.0
18	444.0
19	666.0
20	1102.0
21	1702.0
22	2480.0
23	3654.0
24	5449.0
25	8495.0
26	12280.0
27	14505.0
28	14157.0
29	13717.0
30	14417.0
31	17071.0
32	21203.0
33	30054.0
34	51863.0
35	64625.0
36	71411.0
37	102916.0
38	171797.0
39	231358.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.545265050884865	20.054650870852285	22.925573221073282	19.474510857189568
2	38.2977655033894	21.920604429289597	23.662425774658576	16.11920429266242
3	30.904833916237028	21.886739573419593	31.62743987528391	15.580986635059467
4	27.247663033006557	24.002008536279188	31.13885174783528	17.61147668287898
5	23.896326830891752	30.347932200223042	28.158160554449434	17.59758041443577
6	24.98280482749359	32.41894329972149	28.40934436471282	14.188907508072097
7	75.01053897669748	5.614326001786663	14.881618973661318	4.493516047854545
8	76.59716586188978	5.566681652838517	13.437691584583316	4.3984609006883915
9	70.44368799957961	7.113371363177225	15.881566424747035	6.5613742124961325
10	37.35900834359984	26.800179834062206	22.028621641978408	13.81219018035955
11	27.747111269406606	22.71047299861621	31.1097746819331	18.432641050044083
12	24.958398776194173	20.677180342035044	33.83951561578569	20.524905265985087
13	23.418482037029467	21.265144305157385	35.58869380915402	19.727679848659125
14	20.021253116442555	24.90129562267544	34.242857726733966	20.834593534148034
15	20.188241888491206	23.85568900384775	36.137771575708385	19.81829753195266
16	22.984194454337914	23.134834675276903	33.97182210440886	19.90914876597633
17	22.004682692139266	23.705165558273826	32.61769497106891	21.672456778518004
18	22.968896881513874	23.414628449982192	32.47032446035185	21.146150208152086
19	23.29423304859607	24.582849202132316	31.041577868732812	21.081339880538803
20	24.370551588436904	24.416444306909014	31.22001062655822	19.992993478095862
21	23.631713853645437	25.129124359925033	31.65955310067788	19.579608685751655
22	22.99248550525781	22.681512708079104	31.752155965177586	22.5738458214855
23	22.148900267999462	24.349532022724485	31.61833139680853	21.88323631246752
24	21.73177866397305	23.975617303773596	32.60952069551408	21.68308333673928
25	23.079483152234204	24.662840327204574	30.559529161728044	21.698147358833182
26	21.303213074169875	24.658753189427156	31.335735013341587	22.702298723061386
27	22.861930647110682	23.967559803583836	31.412339652826837	21.758169896478638
28	22.00293106166323	25.312928784543615	31.877339156531537	20.806800997261618
29	23.61396399815495	23.530236061400487	31.32686008559634	21.528939854848222
30	23.869818823021095	23.237013119712266	31.84616013405812	21.04700792320852
31	24.24992263632064	23.899830091843825	30.322008069177723	21.528239202657808
32	24.45614793103247	24.88296189035961	30.071408135739684	20.589482042868237
33	23.515639140766865	25.368630633681516	29.496055912044795	21.619674313506824
34	22.346717736426324	25.227332441948047	31.414558384763154	21.01139143686248
35	22.535543501742872	25.227916318773392	30.395693324536253	21.84084685494748
36	23.12899590702345	26.878769654753633	29.18029532489826	20.811939113324655
37	22.625810858941197	26.948134221604608	29.966660633272806	20.459394286181386
38	21.161681331706266	27.018082665280936	29.52069551407435	22.299540488938455
39	22.166299797394743	25.71557024330147	29.456585838651478	22.66154412065231
40	22.070193671942967	24.970893740256557	31.11783218212286	21.84108040567762
41	20.663284073591832	26.120897535455924	30.222281907408817	22.99353648354343
42	22.144229253396702	26.172629022181482	29.25246250051089	22.430679223910925
43	22.580735568024572	25.009196059999184	29.74513776573694	22.66493060623931
44	22.577932959262913	25.120132656814718	29.854322732076444	22.44761165184593
45	21.928078052654012	24.64380594269833	30.08098371567534	23.34713228897232
46	22.83378778412906	25.333247698065612	29.440120512176755	22.39284400562857
47	22.259136212624583	24.998919827873113	30.940567177948143	21.801376781554165
48	21.390794597971613	24.332366044059345	31.131611675201	23.145227682768045
49	21.723020511592875	24.872919208963676	30.773928731994697	22.63013154744875
50	20.882121107731113	23.718010848431415	32.11334216933596	23.286525874501514
51	21.102826547711494	24.155334590614764	30.6044876772796	24.13735118439414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	454.0
1	847.5
2	1241.0
3	12488.5
4	23736.0
5	15444.0
6	7152.0
7	6791.5
8	6431.0
9	6193.5
10	5956.0
11	5923.0
12	5890.0
13	5535.0
14	5180.0
15	4944.5
16	4709.0
17	4421.0
18	4133.0
19	4031.0
20	3929.0
21	3729.0
22	3529.0
23	3861.0
24	4193.0
25	4599.0
26	6080.5
27	7156.0
28	8370.5
29	9585.0
30	11061.5
31	12538.0
32	14045.5
33	15553.0
34	18066.0
35	20579.0
36	21395.5
37	22212.0
38	24778.5
39	27345.0
40	30713.5
41	34082.0
42	38561.0
43	43040.0
44	52729.5
45	62419.0
46	65879.5
47	69340.0
48	70236.0
49	71132.0
50	64787.5
51	58443.0
52	54365.5
53	50288.0
54	47858.0
55	45428.0
56	43647.5
57	41867.0
58	40455.5
59	39044.0
60	37619.5
61	36195.0
62	33593.0
63	30991.0
64	27830.5
65	24670.0
66	20424.0
67	16178.0
68	13938.0
69	11698.0
70	10257.5
71	8817.0
72	7511.0
73	6205.0
74	5298.5
75	3463.0
76	2534.0
77	2021.0
78	1508.0
79	1188.5
80	869.0
81	654.5
82	440.0
83	284.5
84	129.0
85	94.5
86	60.0
87	52.5
88	45.0
89	29.5
90	14.0
91	10.0
92	6.0
93	5.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	856345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.269841569041045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79227932995437	22.762030420970547
2	7.580266541967321	4.740674676368415
3	3.2192974554341705	3.0200076418513047
4	1.9498952293440144	2.4389165959126515
5	1.4764581616722645	2.308430639940465
6	1.256326335005256	2.3571075272777007
7	1.0737369953473879	2.3502910011931686
8	1.0045842605841107	2.513055253697391
9	0.862790807013533	2.428139865828442
>10	8.682502494508155	48.00404947599307
>50	0.06241943552913555	1.3350386001255048
>100	0.0321670689881637	1.884069985933153
>500	0.004595295569737597	1.0077750731926576
>1k	0.0022976477848687983	1.0736348985557709
>5k	0.0	0.0
>10k+	3.8294129747813303E-4	1.7767783431597732
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14838	1.7327128668936	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT	1951	0.22782873724958982	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	1798	0.20996210639403512	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	1549	0.18088504049185783	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	1364	0.1592815979540956	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT	1156	0.1349923220197467	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1148	0.13405811909919485	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	967	0.11292177802170855	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	926	0.10813398805388015	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC	925	0.10801721268881116	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07532011046949535	0.0
2	0.0	0.0	0.0	0.3851251539975127	0.0
3	0.0	0.0	0.0	0.7125632776509467	0.0
4	0.0	0.0	0.0	1.0269225604166545	0.0
5	0.0	0.0	0.0	1.9028545737991114	0.0
6	0.0	0.0	0.0	2.905020756821141	0.0
7	0.0	0.0	0.0	3.6332319333913317	0.0
8	0.0	0.0	0.0	4.699274241106096	0.0
9	0.0	0.0	0.0	5.34317360409648	0.0
10	0.0	0.0	0.0	6.317313699501953	0.0
11	0.0	0.0	0.0	7.106598392003223	0.0
12	0.0	0.0	0.0	7.886774606029112	0.0
13	0.0	0.0	0.0	8.265477114947831	0.0
14	0.0	0.0	0.0	8.472286286485003	0.0
15	0.0	0.0	0.0	8.680263211672866	0.0
16	0.0	0.0	0.0	8.994856045168712	0.0
17	0.0	0.0	0.0	9.336891089455769	0.0
18	0.0	0.0	0.0	9.759617911005494	0.0
19	0.0	0.0	0.0	10.036959403044333	0.0
20	0.0	0.0	0.0	10.295032959846791	0.0
21	0.0	0.0	0.0	10.562798871949973	0.0
22	0.0	0.0	0.0	10.861393480431369	0.0
23	0.0	0.0	0.0	11.161739719388798	0.0
24	0.0	0.0	0.0	11.422032008127566	0.0
25	0.0	0.0	0.0	11.662589260169675	0.0
26	0.0	0.0	0.0	11.907233649989198	0.0
27	0.0	0.0	0.0	12.156081952951205	0.0
28	0.0	0.0	0.0	12.403762502262524	0.0
29	0.0	0.0	0.0	12.65389533424029	0.0
30	0.0	0.0	0.0	12.966152660434755	0.0
31	0.0	0.0	0.0	13.233451471077661	0.0
32	0.0	0.0	0.0	13.53508223905085	0.0
33	0.0	0.0	0.0	13.786966701504651	0.0
34	1.1677536506898504E-4	0.0	0.0	14.052046780211247	0.0
35	1.1677536506898504E-4	0.0	0.0	14.309302909458221	0.0
36	1.1677536506898504E-4	0.0	0.0	14.57286490841892	0.0
37	1.1677536506898504E-4	0.0	0.0	14.855578067250933	0.0
38	1.1677536506898504E-4	0.0	0.0	15.164448907858398	0.0
39	1.1677536506898504E-4	0.0	0.0	15.473319748465864	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCACG	20	7.0322776E-4	45.0	1
CGTTTTT	6735	0.0	42.995544	1
CGTAAGG	120	0.0	41.249996	2
CGGTCTA	70	0.0	38.57143	31
AACGTAG	65	9.094947E-12	38.076927	1
CGTTATT	285	0.0	37.894737	1
TCGTTTA	30	1.1396791E-4	37.499996	38
TACGATA	30	1.1396791E-4	37.499996	43
GCGTAAG	85	0.0	37.058823	1
GTTTTTT	7950	0.0	37.018867	2
ATAACGG	55	2.748493E-9	36.81818	2
GTACGAG	55	2.748493E-9	36.81818	1
CACGACG	80	0.0	36.5625	26
ACGGTCT	75	1.8189894E-12	36.000004	30
AACGTAA	25	0.0021068095	36.0	42
GATTTCG	25	0.0021068095	36.0	20
TAGCACG	25	0.0021068095	36.0	1
TACGCGG	25	0.0021068095	36.0	2
CGTAGCG	25	0.0021068095	36.0	2
TGCGACG	25	0.0021068095	36.0	1
>>END_MODULE