##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	605958
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.354569128553464	33.0	31.0	34.0	30.0	34.0
2	32.44859379692982	34.0	31.0	34.0	31.0	34.0
3	32.475706237065936	34.0	31.0	34.0	30.0	34.0
4	35.97441406830176	37.0	35.0	37.0	35.0	37.0
5	36.01980335270761	37.0	35.0	37.0	35.0	37.0
6	36.056358691526476	37.0	35.0	37.0	35.0	37.0
7	36.19268497156568	37.0	37.0	37.0	35.0	37.0
8	35.857453816931205	37.0	36.0	37.0	35.0	37.0
9	37.755603523676555	39.0	38.0	39.0	35.0	39.0
10	37.63431128890121	39.0	37.0	39.0	35.0	39.0
11	37.56689737572571	39.0	37.0	39.0	35.0	39.0
12	37.39045280365966	39.0	37.0	39.0	35.0	39.0
13	37.310250875473216	39.0	37.0	39.0	34.0	39.0
14	38.55833737651785	40.0	38.0	41.0	35.0	41.0
15	38.66639602084633	40.0	38.0	41.0	35.0	41.0
16	38.715363110974685	40.0	38.0	41.0	35.0	41.0
17	38.719071288769186	40.0	38.0	41.0	35.0	41.0
18	38.70304542559055	40.0	38.0	41.0	35.0	41.0
19	38.71363196789216	40.0	38.0	41.0	35.0	41.0
20	38.65111773423241	40.0	38.0	41.0	35.0	41.0
21	38.60043435353605	40.0	38.0	41.0	35.0	41.0
22	38.58750771505616	40.0	38.0	41.0	35.0	41.0
23	38.555140785334956	40.0	38.0	41.0	35.0	41.0
24	38.48908175154054	40.0	37.0	41.0	35.0	41.0
25	38.40396199076503	40.0	37.0	41.0	34.0	41.0
26	38.37941738536334	40.0	37.0	41.0	34.0	41.0
27	38.356414140914055	40.0	37.0	41.0	34.0	41.0
28	38.31830093834886	40.0	37.0	41.0	34.0	41.0
29	38.297375395654484	40.0	37.0	41.0	34.0	41.0
30	38.22833925783635	40.0	37.0	41.0	34.0	41.0
31	38.18746018700966	40.0	37.0	41.0	34.0	41.0
32	38.113021034461134	40.0	37.0	41.0	34.0	41.0
33	38.06693368187234	40.0	37.0	41.0	34.0	41.0
34	38.00907653665766	40.0	37.0	41.0	34.0	41.0
35	37.92992583644411	40.0	36.0	41.0	34.0	41.0
36	37.86623165301886	40.0	36.0	41.0	34.0	41.0
37	37.82161965020678	40.0	36.0	41.0	34.0	41.0
38	37.74139131755006	40.0	36.0	41.0	33.0	41.0
39	37.71084134543978	40.0	36.0	41.0	33.0	41.0
40	37.59991121496869	40.0	36.0	41.0	33.0	41.0
41	37.53274814426082	40.0	35.0	41.0	33.0	41.0
42	37.500148525145306	40.0	35.0	41.0	33.0	41.0
43	37.43617214394397	40.0	35.0	41.0	33.0	41.0
44	37.39298268196806	40.0	35.0	41.0	33.0	41.0
45	37.31443928457088	40.0	35.0	41.0	33.0	41.0
46	37.168729515907046	39.0	35.0	41.0	33.0	41.0
47	37.10920393822674	39.0	35.0	41.0	33.0	41.0
48	37.07603497272088	39.0	35.0	41.0	33.0	41.0
49	36.98541648101023	39.0	35.0	41.0	32.0	41.0
50	36.9382019875965	39.0	35.0	41.0	32.0	41.0
51	36.13921591925513	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	4.0
11	5.0
12	14.0
13	15.0
14	18.0
15	23.0
16	35.0
17	60.0
18	94.0
19	196.0
20	297.0
21	538.0
22	745.0
23	1025.0
24	1718.0
25	2362.0
26	3257.0
27	4145.0
28	4559.0
29	5308.0
30	6255.0
31	8104.0
32	10560.0
33	15483.0
34	31448.0
35	50634.0
36	36598.0
37	55446.0
38	104239.0
39	262184.0
40	579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42990768337079	22.18652117803544	23.50988022272171	10.873690915872057
2	32.83197845395225	25.55276108245126	24.75683133154443	16.858429132052056
3	32.862508622709825	24.026087616633497	25.922918750144397	17.18848501051228
4	29.342132623053086	25.85212176421468	25.37832655068503	19.427419062047203
5	25.886282547635314	30.67192775736932	23.596189834939054	19.845599860056307
6	25.993055624317197	34.710326458269385	23.739599114130023	15.557018803283395
7	79.55749408374838	7.067981609286453	8.419890487459526	4.954633819505642
8	79.67697431175098	6.8050921020928845	8.396786575967312	5.121147010188825
9	73.33726099828701	8.918109835995235	10.695790797381997	7.048838368335759
10	40.019605319180535	27.57880909237934	17.713438885203264	14.688146703236857
11	30.911053241313752	25.99767640661564	24.148043263724546	18.943227088346056
12	30.45871165988402	22.309632020701102	27.510322497598843	19.721333821816035
13	24.554837133926775	25.90823126355292	29.142118760706186	20.39481284181412
14	19.571818508873555	29.702058558513954	28.107558609672616	22.618564322939875
15	19.683872479610798	27.390842269596245	33.37360015050548	19.55168510028748
16	21.790454123883173	26.208582112951724	31.16965202208734	20.831311741077766
17	21.841117701226818	26.165179764934205	27.62930764178375	24.364394892055223
18	22.120179946464937	27.80737278821304	29.065545796903415	21.006901468418604
19	23.831849732159654	27.602573115628477	27.345459586307964	21.220117565903905
20	25.972097076034974	26.830902471788477	28.291234706035734	18.90576574614082
21	24.618042834651906	27.15122170183412	27.97900184501236	20.251733618501614
22	22.562619851540866	25.991240316985664	27.68805758814967	23.758082243323795
23	22.608662646586065	26.575439221860258	28.165648444281615	22.650249687272055
24	22.8066961736622	25.68577360147073	29.145089263612327	22.36244096125474
25	22.55304823106552	28.12571168298793	26.443746926354635	22.877493159591918
26	20.58806055865258	29.26787005039953	27.01094795348852	23.133121437459362
27	22.15764128867017	28.36764264189927	28.034946316411368	21.439769753019185
28	20.47056066592074	27.726344070051063	29.543631736853047	22.25946352717515
29	20.839398110100042	27.0206846019031	28.227038837675217	23.91287845032164
30	21.56519098683407	27.65307166503289	27.822555358622214	22.959181989510824
31	23.946214094046123	28.134788219645586	25.19828106898498	22.720716617323315
32	23.70956402919014	28.473425550945773	24.960805864432846	22.856204555431233
33	22.457992138068974	28.69901874387334	25.765152040240412	23.07783707781727
34	22.673848682581962	27.752418484449414	26.64673129160767	22.92700154136095
35	21.553804059027193	27.06771756458368	26.467180893725306	24.911297482663816
36	22.119189778829558	29.27711161499642	25.978863221543406	22.624835384630618
37	22.977170034886907	28.304601969113367	26.30842401618594	22.409803979813784
38	21.842933008558347	27.866782846335887	25.726535502460568	24.5637486426452
39	23.407397872459807	25.979853389178786	27.54332808544487	23.069420652916538
40	22.783262206291525	26.401499773911723	29.11043339637401	21.704804623422746
41	20.258004680192357	28.486792814023413	27.25106360506834	24.00413890071589
42	21.583344060149383	28.08428967024117	26.39522871222098	23.937137557388468
43	22.84828321434819	26.726109730377352	26.63699464319309	23.788612412081363
44	22.056644189861345	26.13035886975665	26.491274972852903	25.321721967529104
45	21.835506751292993	26.057086464738482	26.271787813676855	25.83561897029167
46	22.975189699616145	27.272517237168252	26.2079220011948	23.5443710620208
47	21.76999065941864	27.321695563058825	28.644394495988173	22.263919281534363
48	22.219361737942233	25.47552800689157	27.880315137352753	24.424795117813446
49	21.439274669201495	26.194224682238705	28.372593480076176	23.993907168483624
50	20.697969166179835	24.81525122203189	28.860746124318847	25.626033487469428
51	20.43012882080936	25.669435835486947	26.49144000079213	27.408995342911556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	359.0
1	710.5
2	1062.0
3	1720.0
4	2378.0
5	1732.5
6	1087.0
7	1083.0
8	1079.0
9	1063.5
10	1048.0
11	1065.5
12	1083.0
13	1065.0
14	1047.0
15	1128.0
16	1209.0
17	1182.5
18	1156.0
19	1311.0
20	1466.0
21	1553.5
22	1641.0
23	1981.5
24	2322.0
25	2734.0
26	4530.5
27	5915.0
28	7266.0
29	8617.0
30	10303.0
31	11989.0
32	12564.0
33	13139.0
34	15308.0
35	17477.0
36	18414.5
37	19352.0
38	21008.0
39	22664.0
40	26267.0
41	29870.0
42	32617.5
43	35365.0
44	38743.0
45	42121.0
46	47302.0
47	52483.0
48	55212.5
49	57942.0
50	55924.5
51	53907.0
52	48665.5
53	43424.0
54	38489.5
55	33555.0
56	30139.0
57	26723.0
58	26849.5
59	26976.0
60	25060.0
61	23144.0
62	21576.5
63	20009.0
64	17707.5
65	15406.0
66	12092.5
67	8779.0
68	7591.5
69	6404.0
70	5360.5
71	4317.0
72	3493.0
73	2669.0
74	2246.5
75	1446.5
76	1069.0
77	696.5
78	324.0
79	282.5
80	241.0
81	147.0
82	53.0
83	80.0
84	107.0
85	56.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	605958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.75692169714535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.72850992425967	18.92477201799184
2	11.788428394204892	6.308441109522906
3	4.360177198107952	3.4999475962635924
4	2.1702440246038948	2.3227619772009604
5	1.2145128078364986	1.6248312049730664
6	0.8579598787660507	1.3773819177261324
7	0.5994980625008939	1.122850590114874
8	0.48504902704897085	1.038273506882068
9	0.37133134585024313	0.89421153701495
>10	6.543124549662739	44.82224767522364
>50	0.8138622433016597	13.232277071085683
>100	0.05795496832086856	2.954827263498442
>500	0.008101232130874101	1.4091329611097192
>1k	0.0012463434047498617	0.4680435713921408
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGC	1653	0.2727911835473746	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1154	0.19044224187154885	No Hit
CTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGCT	900	0.14852514530710048	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	854	0.14093386010251535	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	788	0.130042016113328	No Hit
CCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGC	777	0.12822670878179676	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCC	692	0.11419933394723727	No Hit
TCTGTCTCTTATACACATCTGACGCACGCTGAATCGTATGCCGTCTTCTGC	630	0.10396760171497035	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	621	0.10248235026189934	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15331095554477372	0.0
2	0.0	0.0	0.0	0.7543427102208404	0.0
3	0.0	0.0	0.0	1.072846632934956	0.0
4	0.0	0.0	0.0	1.545981734707686	0.0
5	0.0	0.0	0.0	2.851352734017869	0.0
6	0.0	0.0	0.0	3.5449651626020287	0.0
7	0.0	0.0	0.0	4.195835354925589	0.0
8	0.0	0.0	0.0	5.220328801666122	0.0
9	0.0	0.0	0.0	5.637189376161384	0.0
10	0.0	0.0	0.0	6.75508863650616	0.0
11	0.0	0.0	0.0	8.199743216526558	0.0
12	0.0	0.0	0.0	9.399166278851009	0.0
13	0.0	0.0	0.0	9.85662372639688	0.0
14	0.0	0.0	0.0	10.03551401252232	0.0
15	0.0	0.0	0.0	10.427125312315376	0.0
16	0.0	0.0	0.0	11.1430165126956	0.0
17	0.0	0.0	0.0	11.994230623244515	0.0
18	0.0	0.0	0.0	12.915416580027	0.0
19	1.6502793923011164E-4	0.0	0.0	13.491364087940088	0.0
20	1.6502793923011164E-4	0.0	0.0	14.079523663356206	0.0
21	1.6502793923011164E-4	0.0	0.0	14.756303242138895	0.0
22	1.6502793923011164E-4	0.0	0.0	15.464603157314533	0.0
23	1.6502793923011164E-4	0.0	0.0	16.163661507893288	0.0
24	1.6502793923011164E-4	0.0	0.0	16.71799035576723	0.0
25	1.6502793923011164E-4	0.0	0.0	17.25004043184511	0.0
26	1.6502793923011164E-4	0.0	0.0	17.72218536598246	0.0
27	1.6502793923011164E-4	0.0	0.0	18.184758679644464	0.0
28	1.6502793923011164E-4	0.0	0.0	18.645846741853397	0.0
29	1.6502793923011164E-4	0.0	0.0	19.106604748183866	0.0
30	1.6502793923011164E-4	0.0	0.0	19.61654108040491	0.0
31	1.6502793923011164E-4	0.0	0.0	20.158327804897368	0.0
32	1.6502793923011164E-4	0.0	0.0	20.670409500328404	0.0
33	1.6502793923011164E-4	0.0	0.0	21.158562144571075	0.0
34	1.6502793923011164E-4	0.0	0.0	21.66734328121751	0.0
35	1.6502793923011164E-4	0.0	0.0	22.19675291026771	0.0
36	1.6502793923011164E-4	0.0	0.0	22.702893599886462	0.0
37	1.6502793923011164E-4	0.0	0.0	23.19220143970374	0.0
38	1.6502793923011164E-4	0.0	0.0	23.64932883137115	0.0
39	1.6502793923011164E-4	0.0	0.0	24.15233399014453	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAA	25	3.888614E-5	45.000004	1
CACGACG	25	3.888614E-5	45.000004	26
TATTGCG	25	3.888614E-5	45.000004	1
CTCGAAC	25	3.888614E-5	45.000004	12
ACATCCG	25	3.888614E-5	45.000004	16
ACGACCC	25	3.888614E-5	45.000004	34
ACTATCG	20	7.030643E-4	45.0	26
CTATCGA	20	7.030643E-4	45.0	27
GCGAAGT	20	7.030643E-4	45.0	43
CTATACG	20	7.030643E-4	45.0	1
GGCGTAT	20	7.030643E-4	45.0	8
CGACCTT	20	7.030643E-4	45.0	29
ACGGGTA	70	0.0	45.0	5
ATCCGAT	20	7.030643E-4	45.0	19
TCGGAAC	20	7.030643E-4	45.0	33
GAGCGTA	20	7.030643E-4	45.0	9
CGTAAAT	20	7.030643E-4	45.0	14
TTCGGAA	20	7.030643E-4	45.0	32
TAGGCGA	20	7.030643E-4	45.0	20
TAGCTAA	20	7.030643E-4	45.0	20
>>END_MODULE