##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742004
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37502358477852	33.0	31.0	34.0	30.0	34.0
2	32.469072943003006	34.0	31.0	34.0	31.0	34.0
3	32.4947439636444	34.0	31.0	34.0	31.0	34.0
4	35.982272332763706	37.0	35.0	37.0	35.0	37.0
5	36.02166295599485	37.0	35.0	37.0	35.0	37.0
6	36.044401377890146	37.0	35.0	37.0	35.0	37.0
7	36.16841149104317	37.0	36.0	37.0	35.0	37.0
8	35.82571252985159	37.0	35.0	37.0	35.0	37.0
9	37.70974145691937	39.0	38.0	39.0	35.0	39.0
10	37.58303054970054	39.0	37.0	39.0	35.0	39.0
11	37.548831273146774	39.0	37.0	39.0	35.0	39.0
12	37.42101390289001	39.0	37.0	39.0	35.0	39.0
13	37.37363949520488	39.0	37.0	39.0	35.0	39.0
14	38.62813408013973	40.0	38.0	41.0	35.0	41.0
15	38.71028323297448	40.0	38.0	41.0	35.0	41.0
16	38.765423097449606	40.0	38.0	41.0	35.0	41.0
17	38.78496746648266	40.0	38.0	41.0	35.0	41.0
18	38.773503916420935	40.0	38.0	41.0	35.0	41.0
19	38.76428294187093	40.0	38.0	41.0	35.0	41.0
20	38.70515927137859	40.0	38.0	41.0	35.0	41.0
21	38.65526735704929	40.0	38.0	41.0	35.0	41.0
22	38.6234993342354	40.0	38.0	41.0	35.0	41.0
23	38.61484035126495	40.0	38.0	41.0	35.0	41.0
24	38.54917898016722	40.0	38.0	41.0	35.0	41.0
25	38.46607700228031	40.0	38.0	41.0	34.0	41.0
26	38.45119298548256	40.0	38.0	41.0	35.0	41.0
27	38.41701931525976	40.0	37.0	41.0	35.0	41.0
28	38.368995315389135	40.0	37.0	41.0	34.0	41.0
29	38.34804259815311	40.0	37.0	41.0	34.0	41.0
30	38.26018727661845	40.0	37.0	41.0	34.0	41.0
31	38.19941941013795	40.0	37.0	41.0	34.0	41.0
32	38.143545048274675	40.0	37.0	41.0	34.0	41.0
33	38.10132964242781	40.0	37.0	41.0	34.0	41.0
34	38.02828017099638	40.0	37.0	41.0	34.0	41.0
35	37.94603128824103	40.0	37.0	41.0	34.0	41.0
36	37.908423674265904	40.0	37.0	41.0	34.0	41.0
37	37.85484714368117	40.0	37.0	41.0	34.0	41.0
38	37.80867892895456	40.0	36.0	41.0	33.0	41.0
39	37.76427755106442	40.0	36.0	41.0	33.0	41.0
40	37.64657602923973	40.0	36.0	41.0	33.0	41.0
41	37.57463032544299	40.0	36.0	41.0	33.0	41.0
42	37.56082986075547	40.0	36.0	41.0	33.0	41.0
43	37.50459970566197	40.0	35.0	41.0	33.0	41.0
44	37.45435738890895	40.0	35.0	41.0	33.0	41.0
45	37.37974862669204	40.0	35.0	41.0	33.0	41.0
46	37.22613624724395	40.0	35.0	41.0	33.0	41.0
47	37.184144559867605	39.0	35.0	41.0	32.0	41.0
48	37.136642120527654	39.0	35.0	41.0	32.0	41.0
49	37.033406827995535	39.0	35.0	41.0	32.0	41.0
50	36.97515916356246	39.0	35.0	41.0	32.0	41.0
51	36.221195573069686	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	7.0
10	14.0
11	12.0
12	16.0
13	27.0
14	32.0
15	33.0
16	42.0
17	63.0
18	130.0
19	246.0
20	348.0
21	587.0
22	916.0
23	1319.0
24	2038.0
25	2980.0
26	3979.0
27	4844.0
28	5664.0
29	6670.0
30	7966.0
31	9860.0
32	13178.0
33	18346.0
34	36908.0
35	56470.0
36	43686.0
37	68532.0
38	131538.0
39	324864.0
40	681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.638303836637	21.03883537015973	23.907013978361302	10.415846814841968
2	33.525830049433694	24.56913978900383	24.04919650028841	17.855833661274062
3	33.47839095207034	24.24744341000857	24.785580670724148	17.488584967196942
4	29.999299195152584	26.12964350596493	24.46698400547706	19.40407329340543
5	26.649721564843315	30.319647872518207	22.802302952544732	20.228327610093746
6	26.699586525140028	35.24347577641091	22.812949795418895	15.243987903030174
7	79.15294257173817	7.2643813240898965	8.769090193583862	4.813585910588083
8	79.41978749440703	7.023816583199012	8.187961250882744	5.368434671511205
9	72.6215761640099	10.038490358542541	10.615306656028809	6.724626821418752
10	39.68711758966259	27.681252392170393	18.366073498256075	14.265556519910943
11	31.423819817682926	27.180311696432902	23.16025250537733	18.235615980506843
12	30.46964706389723	23.669279410892663	26.761176489614613	19.099897035595497
13	26.021827375593666	25.900668999088953	27.57788367717694	20.49961994814044
14	20.486412472169963	29.61493469037903	28.18313647904863	21.715516358402382
15	20.015255982447535	28.179497684648602	32.2921439776605	19.513102355243365
16	22.72642735079595	26.9007983784454	30.85805467355971	19.514719597198937
17	22.3019013374591	27.361038484967736	26.990555306979473	23.34650487059369
18	22.90041563118258	27.97895968215805	28.063191034010597	21.057433652648772
19	24.740567436294146	28.395534255880023	26.36373927903354	20.500159028792297
20	26.818723349200273	27.431658050360912	26.933008447393814	18.816610153044998
21	25.461183497663086	27.36845084393076	27.320068355426656	19.8502973029795
22	23.65795871720368	26.83314375663743	26.564412051687054	22.944485474471836
23	23.565371615247358	27.533679063724726	27.593247475754843	21.307701845273073
24	23.24313615560024	25.461587808151975	29.081514385367196	22.21376165088059
25	22.66780232990658	27.7521414978895	26.711850609969755	22.868205562234163
26	21.613630115201534	29.657522061875678	26.79244316742228	21.93640465550051
27	22.534918949223993	29.442428881785006	26.860636869882104	21.162015299108898
28	21.218483997390848	29.36870960264365	28.032732977180718	21.380073422784783
29	22.90001132069369	28.0976921957294	27.136241853143652	21.86605463043326
30	22.012280257249287	28.290952609419897	26.248241249373315	23.4485258839575
31	23.033299011865164	29.94889515420402	24.75296629128684	22.264839542643973
32	23.777365081589856	30.012371900960105	24.86159104263589	21.348671974814152
33	23.22561603441491	29.383264780243774	25.007142818637096	22.38397636670422
34	22.608907768691274	29.100921288834027	26.112258154942563	22.177912787532144
35	23.17709877574784	28.012247912410178	25.57129072080474	23.239362591037246
36	22.513625263475667	29.91614600460375	24.60269755958189	22.967531172338692
37	22.25217114732535	30.782179071810933	25.73934911402095	21.226300666842764
38	21.44570649214829	30.917892625915762	24.677899310515848	22.9585015714201
39	23.240305982177993	27.404299707279208	27.028695263098314	22.326699047444485
40	23.695829132996586	27.006592956372206	28.15375658352246	21.14382132710875
41	20.263233082301443	28.49984636201422	27.317103411841448	23.919817143842888
42	21.330073692325108	27.804432321119567	26.644465528487714	24.22102845806761
43	22.431145923741653	27.832868825504985	25.953768443296806	23.782216807456564
44	22.37198182220042	28.1656163578633	25.4412375135444	24.021164306391878
45	22.513760033638633	27.727343787904108	24.950539350192184	24.808356828265076
46	21.78977471819559	29.283265319324425	25.826006328806855	23.100953633673132
47	21.588832405216145	28.762917720120107	27.126538401410233	22.521711473253514
48	21.769424423588013	26.897294354208334	27.28516288321896	24.048118338984693
49	21.273065913391303	26.971013633349685	28.17545457975968	23.580465873499335
50	20.636276893386018	27.211174063751674	27.352682734863965	24.79986630799834
51	20.02374650271427	28.020199352025056	25.484094425366976	26.471959719893697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	349.0
1	775.0
2	1201.0
3	1791.5
4	2382.0
5	1815.0
6	1248.0
7	1214.0
8	1180.0
9	1209.5
10	1239.0
11	1205.0
12	1171.0
13	1271.0
14	1371.0
15	1294.0
16	1217.0
17	1398.0
18	1579.0
19	1638.5
20	1698.0
21	2416.0
22	3134.0
23	3397.5
24	3661.0
25	3883.5
26	5703.0
27	7300.0
28	9070.0
29	10840.0
30	12658.0
31	14476.0
32	15454.5
33	16433.0
34	18180.0
35	19927.0
36	22501.5
37	25076.0
38	27557.5
39	30039.0
40	33582.5
41	37126.0
42	41162.0
43	45198.0
44	48639.0
45	52080.0
46	57766.5
47	63453.0
48	65850.5
49	68248.0
50	67316.0
51	66384.0
52	58530.0
53	50676.0
54	45978.0
55	41280.0
56	37794.5
57	34309.0
58	32469.5
59	30630.0
60	30854.0
61	31078.0
62	28662.0
63	26246.0
64	22119.0
65	17992.0
66	14213.0
67	10434.0
68	8456.0
69	6478.0
70	5604.0
71	4730.0
72	3811.5
73	2893.0
74	2255.0
75	1218.0
76	819.0
77	624.5
78	430.0
79	319.0
80	208.0
81	125.0
82	42.0
83	24.0
84	6.0
85	12.5
86	19.0
87	9.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	742004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.059708800716948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59544968634741	18.657565702825945
2	11.045115507981256	5.756649876165487
3	4.029572735779837	3.150284762571927
4	2.007432728924938	2.092524494112497
5	1.1699928697410966	1.5244836742184067
6	0.7861963008458005	1.2292827996145446
7	0.6014435472826443	1.097141059157916
8	0.45939933924421145	0.9577450403156742
9	0.40206969887881483	0.9430037343336476
>10	6.891408588977008	45.48164014119786
>50	0.937058977151953	14.75905432111043
>100	0.0696250524922595	3.20959330615146
>500	0.0047114697175213195	0.8810113304342301
>1k	5.234966352801466E-4	0.2600197577899725
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	1906	0.2568719306095385	Illumina Single End Adapter 1 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	988	0.1331529210085121	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	949	0.12789688465291293	No Hit
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	893	0.12034975552692438	Illumina Single End Adapter 1 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3477016296408104E-4	0.0	0.0	0.12681872334920027	0.0
2	1.3477016296408104E-4	0.0	0.0	0.6309939029978275	0.0
3	1.3477016296408104E-4	0.0	0.0	0.8768146802443113	0.0
4	1.3477016296408104E-4	0.0	0.0	1.225330321669425	0.0
5	1.3477016296408104E-4	0.0	0.0	2.1970231966404494	0.0
6	1.3477016296408104E-4	0.0	0.0	2.7475593123487205	0.0
7	1.3477016296408104E-4	0.0	0.0	3.250386790367707	0.0
8	1.3477016296408104E-4	0.0	0.0	4.025584767737101	0.0
9	1.3477016296408104E-4	0.0	0.0	4.3383863159767335	0.0
10	1.3477016296408104E-4	0.0	0.0	5.162371092339125	0.0
11	1.3477016296408104E-4	0.0	0.0	6.358591058808308	0.0
12	1.3477016296408104E-4	0.0	0.0	7.2627640821343284	0.0
13	1.3477016296408104E-4	0.0	0.0	7.681360208300764	0.0
14	1.3477016296408104E-4	0.0	0.0	7.846184117605835	0.0
15	1.3477016296408104E-4	0.0	0.0	8.183244295179001	0.0
16	1.3477016296408104E-4	0.0	0.0	8.847526428428957	0.0
17	1.3477016296408104E-4	0.0	0.0	9.63687527290958	0.0
18	1.3477016296408104E-4	0.0	0.0	10.442261766782929	0.0
19	1.3477016296408104E-4	0.0	0.0	10.947515107735269	0.0
20	1.3477016296408104E-4	0.0	0.0	11.439156662228235	0.0
21	1.3477016296408104E-4	0.0	0.0	12.067185621640853	0.0
22	1.3477016296408104E-4	0.0	0.0	12.743866609883504	0.0
23	1.3477016296408104E-4	0.0	0.0	13.33779871806621	0.0
24	1.3477016296408104E-4	0.0	0.0	13.837256942011095	0.0
25	1.3477016296408104E-4	0.0	0.0	14.296823197718611	0.0
26	1.3477016296408104E-4	0.0	0.0	14.711106678670197	0.0
27	1.3477016296408104E-4	0.0	0.0	15.12565969994771	0.0
28	1.3477016296408104E-4	0.0	0.0	15.543986285788217	0.0
29	1.3477016296408104E-4	0.0	0.0	16.003552541495733	0.0
30	1.3477016296408104E-4	0.0	0.0	16.5300995681964	0.0
31	1.3477016296408104E-4	0.0	0.0	17.022684513830114	0.0
32	1.3477016296408104E-4	0.0	0.0	17.522951358752785	0.0
33	1.3477016296408104E-4	0.0	0.0	17.98979520326036	0.0
34	1.3477016296408104E-4	0.0	0.0	18.44760944684934	0.0
35	1.3477016296408104E-4	0.0	0.0	18.91943978738659	0.0
36	1.3477016296408104E-4	0.0	0.0	19.388844264990485	0.0
37	1.3477016296408104E-4	0.0	0.0	19.862157077320337	0.0
38	1.3477016296408104E-4	0.0	0.0	20.32253195400564	0.0
39	1.3477016296408104E-4	0.0	0.0	20.78856717753543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCAT	25	3.8894636E-5	45.000004	36
TAACGGC	20	7.0316694E-4	45.0	24
TACGATG	20	7.0316694E-4	45.0	40
CGCGTGA	20	7.0316694E-4	45.0	40
GCGTAAT	20	7.0316694E-4	45.0	1
AACGGGC	130	0.0	41.53846	4
TACGAGG	65	0.0	41.53846	2
TCGGCGT	45	1.9266736E-8	40.0	4
CGATATG	40	3.456007E-7	39.375	10
AGCGATC	75	0.0	39.000004	14
TATTGCG	35	6.2452964E-6	38.571426	1
ATGCGAG	35	6.2452964E-6	38.571426	1
GCGTAAG	35	6.2452964E-6	38.571426	1
TAACGGG	205	0.0	38.414635	3
TAGCGAG	65	9.094947E-12	38.07692	1
GTGTTCG	30	1.1395322E-4	37.499996	1
GTCATCG	30	1.1395322E-4	37.499996	43
CTATGCG	30	1.1395322E-4	37.499996	1
ACCGCAA	30	1.1395322E-4	37.499996	33
CGCATGT	30	1.1395322E-4	37.499996	38
>>END_MODULE