FastQCFastQC Report
Sat 18 Jun 2016
SRR3551228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722655
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC23600.3265735378569304No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.32214542208937874No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC20760.28727401041990996No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT19690.2724674983221593No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC13570.18777978426773495No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC10430.1443288982986349No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG9380.1297991434363562No Hit
ACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC8070.11167154451294187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG302.164379E-645.0000041
AAGCGCG302.164379E-645.0000041
TATTGCG351.2110831E-745.0000041
AGTGCCG302.164379E-645.0000041
GACGGCG302.164379E-645.0000049
GCGGACG302.164379E-645.0000041
GACTGCG302.164379E-645.0000041
CAGCGCG253.8893628E-545.01
GAAGCGC253.8893628E-545.020
TTCAGCG207.031546E-445.01
CGCGGAG207.031546E-445.010
TCCGAGT207.031546E-445.015
TTTCGCG253.8893628E-545.01
CGCATGG453.8380676E-1045.02
TAGCGGA253.8893628E-545.03
GACCGGA207.031546E-445.041
GCGCCGA253.8893628E-545.09
CACGATA207.031546E-445.010
CGATTCG207.031546E-445.010
CACTGCG207.031546E-445.01