##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	722655
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32221184382589	33.0	31.0	34.0	30.0	34.0
2	32.439839204046194	34.0	31.0	34.0	30.0	34.0
3	32.45095100705039	34.0	31.0	34.0	30.0	34.0
4	35.957101244715666	37.0	35.0	37.0	35.0	37.0
5	36.018451404888914	37.0	35.0	37.0	35.0	37.0
6	36.06759933855021	37.0	35.0	37.0	35.0	37.0
7	36.27468709135064	37.0	37.0	37.0	35.0	37.0
8	36.087160539953366	37.0	37.0	37.0	35.0	37.0
9	37.95497713293342	39.0	38.0	39.0	35.0	39.0
10	37.660434093723836	39.0	37.0	39.0	35.0	39.0
11	37.59679653499941	39.0	37.0	39.0	35.0	39.0
12	37.53369173395327	39.0	37.0	39.0	35.0	39.0
13	37.527366447336554	39.0	37.0	39.0	35.0	39.0
14	38.81040192069521	40.0	38.0	41.0	35.0	41.0
15	38.88001743570584	40.0	38.0	41.0	35.0	41.0
16	38.9310556212854	40.0	38.0	41.0	35.0	41.0
17	38.96153904698646	40.0	38.0	41.0	35.0	41.0
18	38.96144356574022	40.0	38.0	41.0	35.0	41.0
19	38.92393050625817	40.0	38.0	41.0	35.0	41.0
20	38.85021898416257	40.0	38.0	41.0	35.0	41.0
21	38.817091143076574	40.0	38.0	41.0	35.0	41.0
22	38.81507081525763	40.0	38.0	41.0	35.0	41.0
23	38.7990036739523	40.0	38.0	41.0	35.0	41.0
24	38.7581930520096	40.0	38.0	41.0	35.0	41.0
25	38.69904726321689	40.0	38.0	41.0	35.0	41.0
26	38.69359929703662	40.0	38.0	41.0	35.0	41.0
27	38.660497747887995	40.0	38.0	41.0	35.0	41.0
28	38.61295500619244	40.0	38.0	41.0	35.0	41.0
29	38.5982273699068	40.0	38.0	41.0	35.0	41.0
30	38.52308224533145	40.0	38.0	41.0	35.0	41.0
31	38.44668479426559	40.0	38.0	41.0	35.0	41.0
32	38.36111699220236	40.0	38.0	41.0	34.0	41.0
33	38.319837266745544	40.0	38.0	41.0	34.0	41.0
34	38.25489203008351	40.0	38.0	41.0	34.0	41.0
35	38.19817478603206	40.0	38.0	41.0	34.0	41.0
36	38.15454124028755	40.0	38.0	41.0	34.0	41.0
37	38.08854709370308	40.0	38.0	41.0	34.0	41.0
38	38.04481945049851	40.0	37.0	41.0	34.0	41.0
39	38.0288920715971	40.0	37.0	41.0	34.0	41.0
40	37.9917304938041	40.0	37.0	41.0	34.0	41.0
41	37.942527208695715	40.0	37.0	41.0	34.0	41.0
42	37.9320394932575	40.0	37.0	41.0	34.0	41.0
43	37.860595996706586	40.0	37.0	41.0	34.0	41.0
44	37.805685977402774	40.0	37.0	41.0	33.0	41.0
45	37.76136745750047	40.0	37.0	41.0	33.0	41.0
46	37.65332558413074	40.0	37.0	41.0	33.0	41.0
47	37.621383647798744	40.0	36.0	41.0	33.0	41.0
48	37.59288457147601	40.0	36.0	41.0	33.0	41.0
49	37.53415253475033	40.0	36.0	41.0	33.0	41.0
50	37.493579924030136	40.0	36.0	41.0	33.0	41.0
51	36.74789491527769	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	8.0
12	4.0
13	10.0
14	16.0
15	22.0
16	18.0
17	30.0
18	81.0
19	144.0
20	247.0
21	404.0
22	581.0
23	983.0
24	1667.0
25	2719.0
26	3973.0
27	4860.0
28	5200.0
29	5627.0
30	6878.0
31	8594.0
32	11389.0
33	16006.0
34	29873.0
35	47485.0
36	38660.0
37	61035.0
38	127171.0
39	348250.0
40	713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.64983982674997	23.522842850322768	26.897067065197085	10.930250257730176
2	33.402107506348116	23.86934290913368	25.46470999301188	17.26383959150632
3	30.893441545412404	23.3299430571988	28.762134075042724	17.01448132234607
4	28.64077602728826	25.347918439642708	27.43771232469159	18.573593208377442
5	24.73960603607531	29.681798368516098	26.549459977444283	19.029135617964315
6	24.87217275186638	34.089987615113714	25.584822633206716	15.453016999813189
7	82.08398198310397	5.136199154506645	8.251378596979194	4.528440265410189
8	83.84734070891365	3.5826224131847146	8.044225806228422	4.525811071673205
9	77.77182749721513	6.841715618102691	10.002698383045852	5.383758501636327
10	36.3174682248099	31.876621624426594	18.558925074897424	13.246985075866077
11	24.96543994022044	25.733441268655167	29.49720129245629	19.803917498668106
12	24.554040309691345	22.373608430025392	32.764320457202956	20.308030803080307
13	22.766603704395596	24.647861012516344	34.08860382893636	18.496931454151706
14	19.253585735932084	28.6043824508237	31.67666452179809	20.465367291446128
15	18.387889103375745	28.45258110716732	34.59852903529347	18.561000754163466
16	20.81449654399402	26.633317419792295	32.49697296773702	20.05521306847666
17	20.536078765109213	27.49292539316825	29.27980848399305	22.691187357729483
18	21.329265001971894	27.151130207360357	31.6128719790218	19.906732811645945
19	21.308923345164704	29.403242211013552	29.875943569199688	19.411890874622053
20	22.348423521597443	28.543910994873073	30.468065674491978	18.639599809037506
21	21.9187579135272	27.770928036199848	31.744885180341935	18.56542886993102
22	20.377773626419245	25.981277373020323	30.225764714836263	23.41518428572417
23	18.983055538258228	27.94320941528115	30.3458773550311	22.72785769142952
24	20.35701683375885	26.64895420359646	31.11595436273187	21.87807459991282
25	19.48163369796099	29.143643924140843	28.28223702873432	23.092485349163848
26	18.138945970068704	31.336253122167562	29.414727636285644	21.110073271478093
27	20.820170067321197	29.8279261888453	29.361451868457284	19.990451875376216
28	18.42677349495956	27.916917477911312	31.635150936477295	22.02115809065183
29	19.518995924749706	26.42574949318831	31.37043264074835	22.68482194131363
30	21.396240252956115	26.901910316817844	29.88646034414762	21.81538908607842
31	20.616061606160617	28.070240986362787	28.205160138655376	23.108537268821223
32	19.590122534266005	28.762964346749143	27.638499699026507	24.008413419958348
33	20.195390608243212	26.976911527630747	29.77963205125544	23.048065812870593
34	18.12081837114529	26.83922480298344	29.803018037652823	25.236938788218445
35	18.390933432965937	27.427195549743654	28.077990188956004	26.103880828334407
36	20.500515460351064	26.773910095412056	30.511654939078813	22.213919505158064
37	19.353633476555203	26.57409137140129	31.481689049408086	22.59058610263542
38	18.579958624793296	25.96536383198068	29.94679342148051	25.507884121745505
39	19.1778926320305	24.5777030533242	31.481965806643558	24.762438508001743
40	20.141976461797125	24.52664134337962	30.60907348596495	24.722308708858307
41	17.801578900028368	26.285987089274965	29.78004718710865	26.13238682358802
42	20.019649763718512	25.76858943756011	30.575447481855107	23.63631331686628
43	21.061225619417286	25.039887636562398	30.758384014502077	23.140502729518232
44	19.469733136835696	25.837778746428103	29.822667801371335	24.869820315364873
45	19.832008358068514	24.648691284222764	29.26278791401153	26.2565124436972
46	21.043513156347082	25.781181891774086	29.2561457403602	23.919159211518636
47	18.427603766665975	25.40949692453522	31.899730853588505	24.2631684552103
48	18.854501802381495	23.712559935238804	31.969335298309705	25.46360296406999
49	19.838788910337573	22.04606624184431	32.85136060775889	25.263784240059227
50	18.717922106676077	22.561941728764072	31.610657921138024	27.109478243421826
51	18.412658875950488	22.73643716572915	29.0734859649487	29.777417993371664
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	418.0
2	596.0
3	2334.0
4	4072.0
5	2821.5
6	1571.0
7	1533.5
8	1496.0
9	1507.0
10	1518.0
11	1505.5
12	1493.0
13	1494.5
14	1496.0
15	1518.0
16	1540.0
17	1656.5
18	1773.0
19	1849.0
20	1925.0
21	2172.0
22	2419.0
23	2913.0
24	3407.0
25	3562.5
26	5293.0
27	6868.0
28	8665.0
29	10462.0
30	11861.5
31	13261.0
32	15166.5
33	17072.0
34	20177.5
35	23283.0
36	25467.0
37	27651.0
38	29223.0
39	30795.0
40	36160.0
41	41525.0
42	45475.5
43	49426.0
44	53207.5
45	56989.0
46	64732.0
47	72475.0
48	79954.5
49	87434.0
50	84582.5
51	81731.0
52	68670.0
53	55609.0
54	45790.5
55	35972.0
56	30205.5
57	24439.0
58	22169.0
59	19899.0
60	18129.5
61	16360.0
62	13760.0
63	11160.0
64	8841.5
65	6523.0
66	4854.0
67	3185.0
68	2365.0
69	1545.0
70	1136.5
71	728.0
72	633.0
73	538.0
74	392.5
75	195.0
76	143.0
77	88.0
78	33.0
79	29.5
80	26.0
81	14.0
82	2.0
83	2.0
84	2.0
85	4.0
86	6.0
87	3.0
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	722655.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.78976939029281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.57950799177809	23.848652987225105
2	12.290089236384452	8.30558562167101
3	4.690677162753863	4.754906988412981
4	2.401621834236371	3.2460099176615604
5	1.4851619505716007	2.509163990852592
6	1.00399951782048	2.035494730507152
7	0.7622186170521019	1.8028633906624902
8	0.5872663888892132	1.5874876680989227
9	0.49234209953954633	1.4972513404116472
>10	5.39235090690389	40.27875285298634
>50	0.26175136053251347	5.394736209047088
>100	0.04928546164846254	2.8603339371616894
>500	0.0012424906297931735	0.3207537249363761
>1k	0.002484981259586347	1.5580066403650417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	2360	0.3265735378569304	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2328	0.32214542208937874	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	2076	0.28727401041990996	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	1969	0.2724674983221593	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	1357	0.18777978426773495	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC	1043	0.1443288982986349	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG	938	0.1297991434363562	No Hit
ACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	807	0.11167154451294187	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.151358532079623E-4	0.0	0.0	0.28021670091537454	0.0
2	4.151358532079623E-4	0.0	0.0	1.2293556399665124	0.0
3	4.151358532079623E-4	0.0	0.0	1.7603143962194963	0.0
4	4.151358532079623E-4	0.0	0.0	2.3848170980620074	0.0
5	4.151358532079623E-4	0.0	0.0	4.0499270052791445	0.0
6	4.151358532079623E-4	0.0	0.0	5.076142834409227	0.0
7	4.151358532079623E-4	0.0	0.0	6.006739038683743	0.0
8	4.151358532079623E-4	0.0	0.0	7.4273339283613895	0.0
9	4.151358532079623E-4	0.0	0.0	8.038137147048038	0.0
10	4.151358532079623E-4	0.0	0.0	9.500107243428745	0.0
11	4.151358532079623E-4	0.0	0.0	11.763566293736291	0.0
12	4.151358532079623E-4	0.0	0.0	13.407919408293031	0.0
13	4.151358532079623E-4	0.0	0.0	14.094277352263529	0.0
14	4.151358532079623E-4	0.0	0.0	14.348894008897744	0.0
15	4.151358532079623E-4	0.0	0.0	14.810525077665	0.0
16	4.151358532079623E-4	0.0	0.0	15.822626287786012	0.0
17	4.151358532079623E-4	0.0	0.0	17.134593962540908	0.0
18	4.151358532079623E-4	0.0	0.0	18.49776172585812	0.0
19	4.151358532079623E-4	0.0	0.0	19.353910233790675	0.0
20	4.151358532079623E-4	0.0	0.0	20.134227259203907	0.0
21	4.151358532079623E-4	0.0	0.0	21.1573987587438	0.0
22	4.151358532079623E-4	0.0	0.0	22.238689277732806	0.0
23	4.151358532079623E-4	0.0	0.0	23.316658709896146	0.0
24	4.151358532079623E-4	0.0	0.0	24.14803744525396	0.0
25	4.151358532079623E-4	0.0	0.0	24.84671108620296	0.0
26	4.151358532079623E-4	0.0	0.0	25.5074689858923	0.0
27	4.151358532079623E-4	0.0	0.0	26.208079927489603	0.0
28	4.151358532079623E-4	0.0	0.0	26.88032325245103	0.0
29	4.151358532079623E-4	0.0	0.0	27.600999093620054	0.0
30	4.151358532079623E-4	0.0	0.0	28.372183130262712	0.0
31	4.151358532079623E-4	0.0	0.0	29.15236177705821	0.0
32	4.151358532079623E-4	0.0	0.0	29.873037618227233	0.0
33	4.151358532079623E-4	0.0	0.0	30.517881976876932	0.0
34	4.151358532079623E-4	0.0	0.0	31.159682005936443	0.0
35	4.151358532079623E-4	0.0	0.0	31.84437940649411	0.0
36	4.151358532079623E-4	0.0	0.0	32.490884308556645	0.0
37	4.151358532079623E-4	0.0	0.0	33.175996844967514	0.0
38	4.151358532079623E-4	0.0	0.0	33.84533421895649	0.0
39	4.151358532079623E-4	0.0	0.0	34.50194076011375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTCG	30	2.164379E-6	45.000004	1
AAGCGCG	30	2.164379E-6	45.000004	1
TATTGCG	35	1.2110831E-7	45.000004	1
AGTGCCG	30	2.164379E-6	45.000004	1
GACGGCG	30	2.164379E-6	45.000004	9
GCGGACG	30	2.164379E-6	45.000004	1
GACTGCG	30	2.164379E-6	45.000004	1
CAGCGCG	25	3.8893628E-5	45.0	1
GAAGCGC	25	3.8893628E-5	45.0	20
TTCAGCG	20	7.031546E-4	45.0	1
CGCGGAG	20	7.031546E-4	45.0	10
TCCGAGT	20	7.031546E-4	45.0	15
TTTCGCG	25	3.8893628E-5	45.0	1
CGCATGG	45	3.8380676E-10	45.0	2
TAGCGGA	25	3.8893628E-5	45.0	3
GACCGGA	20	7.031546E-4	45.0	41
GCGCCGA	25	3.8893628E-5	45.0	9
CACGATA	20	7.031546E-4	45.0	10
CGATTCG	20	7.031546E-4	45.0	10
CACTGCG	20	7.031546E-4	45.0	1
>>END_MODULE