FastQCFastQC Report
Sat 18 Jun 2016
SRR3551226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187169
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58380.4917581237380693No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC35160.2961667631146029Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT18970.15979190831296977Illumina Single End Adapter 2 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC15780.13292126057873815No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC13050.10992537709458383No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC12910.10874610101847336Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG302.1655615E-645.0000041
AGTCGAG302.1655615E-645.0000041
GATACGC207.0333807E-445.08
ACCGCGA207.0333807E-445.040
CCCGTCG207.0333807E-445.043
TGTTACG351.2119744E-745.01
ACGTACG253.890883E-545.01
CGTCATT207.0333807E-445.015
TTCGACC253.890883E-545.028
CTAGCGA207.0333807E-445.014
TTAAGCG950.042.631581
TCGACGG700.041.7857132
TTAGGCG603.6379788E-1241.2500041
CGTTTTT31100.041.237941
TTACGCG556.184564E-1140.9090921
TACGTAG556.184564E-1140.9090921
ACGGGCG1400.040.178575
TTACGGG3250.040.1538473
CCGACGG451.9283107E-840.02
GTTTACG800.039.3751