##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1187169
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33375281868041	33.0	31.0	34.0	30.0	34.0
2	32.441553814157885	34.0	31.0	34.0	30.0	34.0
3	32.4532707643141	34.0	31.0	34.0	30.0	34.0
4	35.96196834654544	37.0	35.0	37.0	35.0	37.0
5	36.01633128897402	37.0	35.0	37.0	35.0	37.0
6	36.06192715611678	37.0	35.0	37.0	35.0	37.0
7	36.237476719826745	37.0	37.0	37.0	35.0	37.0
8	35.964488627988096	37.0	37.0	37.0	35.0	37.0
9	37.8431545971972	39.0	38.0	39.0	35.0	39.0
10	37.61770733568683	39.0	37.0	39.0	35.0	39.0
11	37.56062110786249	39.0	37.0	39.0	35.0	39.0
12	37.4986442536825	39.0	37.0	39.0	35.0	39.0
13	37.50156633133109	39.0	37.0	39.0	35.0	39.0
14	38.794417643991714	40.0	38.0	41.0	35.0	41.0
15	38.85173214597079	40.0	38.0	41.0	35.0	41.0
16	38.9006291437866	40.0	38.0	41.0	35.0	41.0
17	38.9163573172817	40.0	38.0	41.0	35.0	41.0
18	38.883321582689575	40.0	38.0	41.0	35.0	41.0
19	38.85407132430176	40.0	38.0	41.0	35.0	41.0
20	38.77431182923409	40.0	38.0	41.0	35.0	41.0
21	38.7292500056858	40.0	38.0	41.0	35.0	41.0
22	38.71628976160934	40.0	38.0	41.0	35.0	41.0
23	38.689337406889834	40.0	38.0	41.0	35.0	41.0
24	38.641083114535505	40.0	38.0	41.0	35.0	41.0
25	38.580440527001635	40.0	38.0	41.0	35.0	41.0
26	38.58678755931127	40.0	38.0	41.0	35.0	41.0
27	38.55363137009137	40.0	38.0	41.0	35.0	41.0
28	38.50357783938092	40.0	38.0	41.0	35.0	41.0
29	38.50653361063168	40.0	38.0	41.0	35.0	41.0
30	38.422260857552715	40.0	38.0	41.0	35.0	41.0
31	38.36256506023995	40.0	38.0	41.0	34.0	41.0
32	38.28022295056559	40.0	38.0	41.0	34.0	41.0
33	38.199737358371046	40.0	38.0	41.0	34.0	41.0
34	38.11328968327172	40.0	38.0	41.0	34.0	41.0
35	38.02702479596418	40.0	38.0	41.0	34.0	41.0
36	37.97506168035048	40.0	37.0	41.0	34.0	41.0
37	37.92596336326167	40.0	37.0	41.0	34.0	41.0
38	37.871289597353034	40.0	37.0	41.0	34.0	41.0
39	37.822048082454984	40.0	37.0	41.0	33.0	41.0
40	37.75342684992617	40.0	37.0	41.0	33.0	41.0
41	37.68990009004615	40.0	37.0	41.0	33.0	41.0
42	37.65092838509092	40.0	37.0	41.0	33.0	41.0
43	37.58570094064114	40.0	36.0	41.0	33.0	41.0
44	37.54536801415805	40.0	36.0	41.0	33.0	41.0
45	37.502541761114045	40.0	36.0	41.0	33.0	41.0
46	37.387687852361374	40.0	36.0	41.0	33.0	41.0
47	37.353357441105686	40.0	36.0	41.0	33.0	41.0
48	37.31699362095877	40.0	36.0	41.0	33.0	41.0
49	37.25328154626679	40.0	36.0	41.0	33.0	41.0
50	37.19675631691865	40.0	35.0	41.0	33.0	41.0
51	36.43510991274199	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	8.0
9	11.0
10	13.0
11	12.0
12	14.0
13	21.0
14	23.0
15	31.0
16	59.0
17	100.0
18	146.0
19	292.0
20	499.0
21	901.0
22	1407.0
23	2212.0
24	3348.0
25	5449.0
26	7716.0
27	9239.0
28	9721.0
29	10379.0
30	12029.0
31	14919.0
32	19420.0
33	27022.0
34	50759.0
35	77094.0
36	67652.0
37	103917.0
38	206436.0
39	555056.0
40	1263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01828046385982	21.94455886230183	25.022469420950177	11.014691252888174
2	33.33552341747468	25.2114905291496	24.604752987990757	16.848233065384964
3	31.654549604984634	23.89651347028098	27.808087980733998	16.640848944000393
4	27.92685792839941	25.868094601526824	27.46416053653692	18.740886933536842
5	25.00907621408578	29.903240397955134	26.061580112014383	19.026103275944706
6	24.93688767142673	34.72614261322524	25.409019271898103	14.927950443449923
7	79.69396101144824	6.164328751845778	9.524844398733459	4.616865837972521
8	80.63384404410829	5.5123575497675565	8.935795998716275	4.918002407407875
9	74.22338352837717	8.14913462194515	11.5250650918277	6.102416757849977
10	37.052685843380345	29.091393053558505	19.518619505731703	14.337301597329443
11	26.301983963530045	25.717568433811866	28.04832336423879	19.9321242384193
12	24.494574908879866	23.535570756985734	31.58623582657566	20.383618507558737
13	22.805009227835296	24.61730385480079	33.01130673054974	19.56638018681418
14	19.560062636406442	27.935449796953932	31.23135796167184	21.273129604967785
15	19.32125923099407	27.864777466392738	33.573737184848994	19.240226117764195
16	21.62025794137145	27.098416484931796	31.274233070439	20.007092503257752
17	21.94026292802457	27.59034307668074	28.669886090354446	21.799507904940242
18	21.45162146248765	27.119559220296352	31.078894411831847	20.349924905384153
19	21.99147720332994	28.808282561286557	29.358667552808406	19.8415726825751
20	23.022669897883116	27.82746180198438	30.31623972661011	18.833628573522386
21	22.12625161202828	28.013955890020714	31.00737974121629	18.85241275673472
22	21.32703936844712	25.59155436167892	30.114667751600656	22.966738518273306
23	19.970871880920072	27.320962727294933	30.621166826290107	22.086998565494888
24	20.654430835036965	26.518212655485446	31.31508656307569	21.5122699464019
25	19.92690173008224	29.035714375965004	28.643015442620218	22.39436845133254
26	19.192970840714338	29.227936376371016	29.672102287037482	21.906990495877167
27	20.670014126042712	27.844392837077116	30.48959330979835	20.99599972708182
28	18.520867711336802	28.00645906353687	31.24618314662866	22.226490078497672
29	19.76230848345939	25.911980518359222	30.241187227766225	24.084523770415164
30	19.66965107747928	27.38270625327986	30.169419855134354	22.7782228141065
31	20.39145227006433	28.155300551143096	27.533737825027437	23.919509353765132
32	20.324570469747776	28.526351345090717	27.271433132098295	23.877645053063212
33	20.095369740955164	28.191605407486215	27.467529896754378	24.245494954804244
34	18.920558067132816	27.58604714240348	28.379699941625837	25.113694848837863
35	19.713368526300805	27.585710206381737	28.234985920285993	24.46593534703147
36	20.203863139957328	28.601151141918297	27.450430393650777	23.744555324473602
37	19.54970185373776	29.382337308336048	29.219344507816498	21.8486163301097
38	19.93616747068025	29.23181114062109	26.918408415314076	23.91361297338458
39	20.563289641154714	28.671823472479485	27.415136345372897	23.3497505409929
40	21.210543738928493	27.289543443267135	28.626674045565544	22.873238772238828
41	19.5292329904167	27.942525453410592	28.47867489801368	24.049566658159033
42	20.421355341994275	27.008370333120226	29.11868487131992	23.451589453565582
43	20.711373022712014	26.96861188255421	29.009012196241645	23.311002898492127
44	19.49174885799747	27.048971123740596	28.156058657192023	25.303221361069905
45	19.65558399857139	27.059079204392972	27.40250124455743	25.882835552478205
46	21.076443202273644	27.64829607242103	27.42221200182956	23.853048723475766
47	19.79330659746001	26.576249885231167	30.055367011773388	23.57507650553544
48	19.430173800023418	26.535564860605355	29.75246152822387	24.28179981114736
49	19.81276465271583	25.943820972414205	30.007269394669166	24.236144980200798
50	19.357732555348058	25.63729342663092	29.42706556522281	25.57790845279821
51	19.031157316270892	25.344411789728337	27.689991905112077	27.934438988888694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	332.0
1	905.0
2	1478.0
3	5514.0
4	9550.0
5	6367.5
6	3185.0
7	3102.0
8	3019.0
9	3113.0
10	3207.0
11	3173.0
12	3139.0
13	3169.0
14	3199.0
15	3067.5
16	2936.0
17	3009.0
18	3082.0
19	3213.5
20	3345.0
21	4690.5
22	6036.0
23	6314.5
24	6593.0
25	7862.0
26	11215.0
27	13299.0
28	15518.0
29	17737.0
30	20518.0
31	23299.0
32	26563.5
33	29828.0
34	34093.5
35	38359.0
36	41702.0
37	45045.0
38	47851.0
39	50657.0
40	56564.5
41	62472.0
42	68361.0
43	74250.0
44	80141.5
45	86033.0
46	99475.5
47	112918.0
48	120934.5
49	128951.0
50	121548.0
51	114145.0
52	99254.5
53	84364.0
54	72640.5
55	60917.0
56	54593.0
57	48269.0
58	42861.0
59	37453.0
60	34749.0
61	32045.0
62	28420.0
63	24795.0
64	20456.5
65	16118.0
66	13476.5
67	10835.0
68	8968.0
69	7101.0
70	5657.0
71	4213.0
72	3346.5
73	2480.0
74	2076.5
75	1236.5
76	800.0
77	638.5
78	477.0
79	408.5
80	340.0
81	193.5
82	47.0
83	28.5
84	10.0
85	7.0
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1187169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.748774576208273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.8295291649199	20.36262167301252
2	11.79063666934008	6.779327114336661
3	4.402534367902247	3.7970240432049387
4	2.3374995989511547	2.6880099616889592
5	1.3659624708935616	1.9634873577639729
6	0.9571068680164175	1.6509389756366826
7	0.7171823495647637	1.4432679588370956
8	0.5472269852641747	1.2585684193102242
9	0.44257864030361027	1.1451234206099934
>10	5.76975528485974	39.52861326105219
>50	0.7766769152600812	13.99525183600264
>100	0.05677155296920723	3.0482906814624533
>500	0.004458498923874055	0.9223572341551584
>1k	0.001783399569549622	0.9182555899788152
>5k	2.972332615916037E-4	0.4988624729477315
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5838	0.4917581237380693	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3516	0.2961667631146029	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	1897	0.15979190831296977	Illumina Single End Adapter 2 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	1578	0.13292126057873815	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	1305	0.10992537709458383	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1291	0.10874610101847336	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5270201630938813E-4	0.0	0.0	0.16509865065546692	0.0
2	2.5270201630938813E-4	0.0	0.0	0.7598749630423302	0.0
3	3.3693602174585086E-4	0.0	0.0	1.0820700338368	0.0
4	3.3693602174585086E-4	0.0	0.0	1.5750916676564162	0.0
5	3.3693602174585086E-4	0.0	0.0	2.8897317905032898	0.0
6	3.3693602174585086E-4	0.0	0.0	3.7962581570105014	0.0
7	3.3693602174585086E-4	0.0	0.0	4.586794298031704	0.0
8	3.3693602174585086E-4	0.0	0.0	5.871868284970379	0.0
9	3.3693602174585086E-4	0.0	0.0	6.43598341937837	0.0
10	4.2117002718231354E-4	0.0	0.0	7.7080853694798295	0.0
11	4.2117002718231354E-4	0.0	0.0	9.2747536365926	0.0
12	4.2117002718231354E-4	0.0	0.0	10.609525686738788	0.0
13	4.2117002718231354E-4	0.0	0.0	11.103221192601895	0.0
14	4.2117002718231354E-4	0.0	0.0	11.342108832019704	0.0
15	4.2117002718231354E-4	0.0	0.0	11.693111932673444	0.0
16	4.2117002718231354E-4	0.0	0.0	12.412470339100835	0.0
17	4.2117002718231354E-4	0.0	0.0	13.352774541788069	0.0
18	4.2117002718231354E-4	0.0	0.0	14.385736150455411	0.0
19	4.2117002718231354E-4	0.0	0.0	15.066852318414648	0.0
20	4.2117002718231354E-4	0.0	0.0	15.705346079623036	0.0
21	4.2117002718231354E-4	0.0	0.0	16.528396546742712	0.0
22	4.2117002718231354E-4	0.0	0.0	17.3588596063408	0.0
23	4.2117002718231354E-4	0.0	0.0	18.179551521308255	0.0
24	4.2117002718231354E-4	0.0	0.0	18.836576763712664	0.0
25	4.2117002718231354E-4	0.0	0.0	19.432532352175638	0.0
26	4.2117002718231354E-4	0.0	0.0	19.973483135088603	0.0
27	4.2117002718231354E-4	0.0	0.0	20.51477085402331	0.0
28	4.2117002718231354E-4	0.0	0.0	21.106430508209026	0.0
29	4.2117002718231354E-4	0.0	0.0	21.70171222462851	0.0
30	5.054040326187763E-4	0.0	0.0	22.347618578315302	0.0
31	5.054040326187763E-4	0.0	0.0	22.96564347620263	0.0
32	5.054040326187763E-4	0.0	0.0	23.574908037524565	0.0
33	5.054040326187763E-4	0.0	0.0	24.15772312113945	0.0
34	5.054040326187763E-4	0.0	0.0	24.72495491374859	0.0
35	5.054040326187763E-4	0.0	0.0	25.327059584608424	0.0
36	5.054040326187763E-4	0.0	0.0	25.903894053837323	0.0
37	5.896380380552389E-4	0.0	0.0	26.480896991077092	0.0
38	5.896380380552389E-4	0.0	0.0	27.050403101833016	0.0
39	5.896380380552389E-4	0.0	0.0	27.622015062724852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACCG	30	2.1655615E-6	45.000004	1
AGTCGAG	30	2.1655615E-6	45.000004	1
GATACGC	20	7.0333807E-4	45.0	8
ACCGCGA	20	7.0333807E-4	45.0	40
CCCGTCG	20	7.0333807E-4	45.0	43
TGTTACG	35	1.2119744E-7	45.0	1
ACGTACG	25	3.890883E-5	45.0	1
CGTCATT	20	7.0333807E-4	45.0	15
TTCGACC	25	3.890883E-5	45.0	28
CTAGCGA	20	7.0333807E-4	45.0	14
TTAAGCG	95	0.0	42.63158	1
TCGACGG	70	0.0	41.785713	2
TTAGGCG	60	3.6379788E-12	41.250004	1
CGTTTTT	3110	0.0	41.23794	1
TTACGCG	55	6.184564E-11	40.909092	1
TACGTAG	55	6.184564E-11	40.909092	1
ACGGGCG	140	0.0	40.17857	5
TTACGGG	325	0.0	40.153847	3
CCGACGG	45	1.9283107E-8	40.0	2
GTTTACG	80	0.0	39.375	1
>>END_MODULE