SRR3551225_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551225_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431118
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1524	0.3534995059357299	RNA PCR Primer, Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1174	0.27231523619983394	RNA PCR Primer, Index 24 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	1015	0.23543438223409832	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.2140945170463771	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	697	0.1616726743026271	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	588	0.13638957315630523	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	572	0.13267829225409286	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	566	0.13128656191576318	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	557	0.12919896640826875	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	539	0.1250237753932798	RNA PCR Primer, Index 24 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	435	0.10090044952889927	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	25	3.8867354E-5	45.000004	2
CGCATGG	25	3.8867354E-5	45.000004	2
GCGTTAC	25	3.8867354E-5	45.000004	24
GTTACGT	25	3.8867354E-5	45.000004	26
GCGAGCA	25	3.8867354E-5	45.000004	20
TATTTCG	20	7.028378E-4	45.0	17
TAATACG	20	7.028378E-4	45.0	1
CACGGGT	90	0.0	45.0	4
ATCAGCG	20	7.028378E-4	45.0	31
CGTTGAT	45	3.8380676E-10	45.0	25
ACGGGCT	55	1.8189894E-12	45.0	5
TATTGCG	20	7.028378E-4	45.0	1
ACGATTT	20	7.028378E-4	45.0	45
ACGGATA	20	7.028378E-4	45.0	45
ACTACCG	20	7.028378E-4	45.0	1
GTACTGC	20	7.028378E-4	45.0	25
CGTAAGG	40	6.7975634E-9	45.0	2
TTACGAG	20	7.028378E-4	45.0	1
GCGTGCC	20	7.028378E-4	45.0	28
TCGACGG	20	7.028378E-4	45.0	2