##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551225_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431118
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39433751316345	33.0	31.0	34.0	30.0	34.0
2	32.48482550021108	34.0	31.0	34.0	31.0	34.0
3	32.50548341753302	34.0	31.0	34.0	31.0	34.0
4	35.98942284942869	37.0	35.0	37.0	35.0	37.0
5	36.038140369921926	37.0	35.0	37.0	35.0	37.0
6	36.08424607648022	37.0	36.0	37.0	35.0	37.0
7	36.2206820406478	37.0	37.0	37.0	35.0	37.0
8	35.87473499134808	37.0	36.0	37.0	35.0	37.0
9	37.76768077417319	39.0	38.0	39.0	35.0	39.0
10	37.68068834982534	39.0	37.0	39.0	35.0	39.0
11	37.627988161013924	39.0	37.0	39.0	35.0	39.0
12	37.44001410286743	39.0	37.0	39.0	35.0	39.0
13	37.37172885381728	39.0	37.0	39.0	35.0	39.0
14	38.63484707203132	40.0	38.0	41.0	35.0	41.0
15	38.72839454627272	40.0	38.0	41.0	35.0	41.0
16	38.772813939571066	40.0	38.0	41.0	35.0	41.0
17	38.77106267889534	40.0	38.0	41.0	35.0	41.0
18	38.769079463163216	40.0	38.0	41.0	35.0	41.0
19	38.745740145389426	40.0	38.0	41.0	35.0	41.0
20	38.70940902490734	40.0	38.0	41.0	35.0	41.0
21	38.6254784073038	40.0	38.0	41.0	35.0	41.0
22	38.60571351694895	40.0	38.0	41.0	35.0	41.0
23	38.59852986885261	40.0	38.0	41.0	35.0	41.0
24	38.55512643870124	40.0	38.0	41.0	35.0	41.0
25	38.46103155052677	40.0	37.0	41.0	35.0	41.0
26	38.4525072022045	40.0	37.0	41.0	35.0	41.0
27	38.41796909430829	40.0	37.0	41.0	35.0	41.0
28	38.34037548884528	40.0	37.0	41.0	35.0	41.0
29	38.3215894488284	40.0	37.0	41.0	35.0	41.0
30	38.24610431482796	40.0	37.0	41.0	34.0	41.0
31	38.1865220194935	40.0	37.0	41.0	34.0	41.0
32	38.12600030618067	40.0	37.0	41.0	34.0	41.0
33	38.0916524014307	40.0	37.0	41.0	34.0	41.0
34	37.99984459011222	40.0	36.0	41.0	34.0	41.0
35	37.959911207604414	40.0	36.0	41.0	34.0	41.0
36	37.90275516215978	40.0	36.0	41.0	34.0	41.0
37	37.82900505198113	40.0	36.0	41.0	34.0	41.0
38	37.75814278225451	40.0	36.0	41.0	34.0	41.0
39	37.72990225413924	40.0	36.0	41.0	34.0	41.0
40	37.65479752643128	40.0	36.0	41.0	33.0	41.0
41	37.58503936277307	40.0	36.0	41.0	33.0	41.0
42	37.53197268497256	40.0	35.0	41.0	33.0	41.0
43	37.45845916895142	40.0	35.0	41.0	33.0	41.0
44	37.39620475136738	40.0	35.0	41.0	33.0	41.0
45	37.3307539931063	40.0	35.0	41.0	33.0	41.0
46	37.23560602897582	39.0	35.0	41.0	33.0	41.0
47	37.207773741759794	39.0	35.0	41.0	33.0	41.0
48	37.178016227575746	39.0	35.0	41.0	33.0	41.0
49	37.07088778478282	39.0	35.0	41.0	33.0	41.0
50	37.0166914858577	39.0	35.0	41.0	33.0	41.0
51	36.25122124337189	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	7.0
11	8.0
12	9.0
13	4.0
14	11.0
15	17.0
16	25.0
17	42.0
18	64.0
19	104.0
20	242.0
21	308.0
22	537.0
23	749.0
24	1147.0
25	1632.0
26	2323.0
27	2854.0
28	3151.0
29	3571.0
30	4353.0
31	5474.0
32	7236.0
33	10373.0
34	21320.0
35	37763.0
36	25861.0
37	39196.0
38	72435.0
39	189850.0
40	442.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99890053303272	21.839728334237957	23.720651886490472	11.44071924623885
2	33.59938578301068	24.26667408922847	24.459892651199905	17.674047476560943
3	32.96150937794293	23.93892159455184	25.512504697089895	17.587064330415338
4	29.858878543693372	25.526653955529575	25.674641281505295	18.939826219271755
5	26.222287169638008	29.8405540942387	24.2047420891728	19.732416646950487
6	26.07754721445173	33.95590070467946	23.28156096474747	16.68499111612134
7	78.1730291938634	7.146999197435505	9.344773356714404	5.335198251986695
8	78.39663386822168	6.979063736610394	8.513678389675217	6.110624005492696
9	72.0426890085777	9.622191604154779	11.597984774470099	6.7371346127974245
10	39.30895949600806	28.489879800889778	18.16068918486354	14.040471518238625
11	32.15082645586591	25.38794483180939	24.02752842609216	18.43370028623254
12	30.02240685844711	23.644802583051508	27.24799242898696	19.084798129514425
13	24.56636002208212	27.39574779990629	27.51311705843876	20.524775119572833
14	18.982505949647198	30.12678663382183	28.20387921636304	22.686828200167934
15	19.348994938740667	27.729531126049018	33.98837441257382	18.933099522636493
16	21.164043254978914	26.79892743981926	32.1392287030465	19.89780060215533
17	21.984700244480628	27.37116056392913	26.656507035196856	23.987632156393378
18	22.371369323479882	26.947378675907757	28.92433162150502	21.756920379107346
19	23.85402604391373	27.891435755408033	27.064747934440227	21.189790266238013
20	27.336367305470894	25.67139391071586	28.05960317128953	18.93263561252372
21	24.217731572330546	27.316651125677886	28.80278717195756	19.662830130034003
22	22.56226833488743	25.528045685867905	27.42543804712399	24.484247932120674
23	22.001169053484197	27.705639755241023	27.7436803844887	22.549510806786078
24	23.968611841769537	24.79367597734263	29.485198947851863	21.752513233035966
25	21.541666086779028	27.876590631799182	27.38623764259437	23.19550563882742
26	20.69827750175126	29.256491262253025	27.37139251898552	22.67383871701019
27	22.788424514865998	28.519106137994704	27.910224114975485	20.782245232163817
28	19.88527502911036	27.887028609336657	30.611108791560547	21.616587569992436
29	21.634216154277947	25.595312652220503	30.03145310564625	22.739018087855296
30	21.915577637676922	27.7951744070069	27.56461108095695	22.724636874359224
31	23.638307841472635	28.77008150900682	25.926080562630183	21.665530086890364
32	24.326286538720257	27.630254361914837	26.514318585630846	21.52914051373406
33	23.965132515923713	28.21965216019744	25.598096112897167	22.217119210981682
34	20.107719928186714	28.403128609800564	28.485472654818402	23.00367880719432
35	23.299885414202144	25.779484966992793	28.16444685677703	22.75618276202803
36	24.760738359335495	27.581543800073298	26.59387916997203	21.06383867061918
37	23.177181189372746	28.43026735139799	28.562713688595696	19.829837770633564
38	21.83555314322297	27.777081912608615	27.38878914821464	22.998575795953776
39	23.76820267305007	25.54126712408204	28.1175919353866	22.572938267481295
40	23.0732653241108	25.922369281727974	28.797916115773408	22.20644927838782
41	20.94971678287615	26.126257776293265	29.032422677781955	23.89160276304863
42	23.54877318970676	24.691847707588177	29.282702183624902	22.47667691908016
43	25.26013759573945	24.30401885330698	27.935971126234584	22.499872424718987
44	22.40801822238923	25.835850045695146	27.814426676687127	23.9417050552285
45	22.857779076726093	26.587616383449543	26.364475619204025	24.190128920620342
46	22.126424783933864	27.653217912497276	27.524018946089008	22.696338357479856
47	21.60290222166553	25.965512922216195	30.766982589453466	21.66460226666481
48	22.767316604734667	24.127501055395506	29.86351764482114	23.241664695048687
49	22.375312559438484	24.677698449148494	29.896223307771884	23.05076568364114
50	21.18631094039219	24.97784829211492	29.94841319545925	23.887427572033644
51	21.12832217629512	24.654039033396888	27.392036519004076	26.82560227130391
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	316.0
1	663.5
2	1011.0
3	1499.0
4	1987.0
5	1472.5
6	958.0
7	866.0
8	774.0
9	775.0
10	776.0
11	767.5
12	759.0
13	763.0
14	767.0
15	765.5
16	764.0
17	856.5
18	949.0
19	983.0
20	1017.0
21	1294.0
22	1571.0
23	1767.0
24	1963.0
25	2231.5
26	3609.5
27	4719.0
28	5142.0
29	5565.0
30	6756.0
31	7947.0
32	8762.0
33	9577.0
34	11295.5
35	13014.0
36	13726.5
37	14439.0
38	15353.0
39	16267.0
40	17836.0
41	19405.0
42	22703.5
43	26002.0
44	28376.5
45	30751.0
46	34502.0
47	38253.0
48	39942.5
49	41632.0
50	40935.0
51	40238.0
52	35007.0
53	29776.0
54	25969.0
55	22162.0
56	21055.0
57	19948.0
58	18271.5
59	16595.0
60	16451.5
61	16308.0
62	15191.5
63	14075.0
64	11971.5
65	9868.0
66	8719.0
67	7570.0
68	5987.0
69	4404.0
70	3509.5
71	2615.0
72	2286.5
73	1958.0
74	1518.0
75	752.5
76	427.0
77	339.5
78	252.0
79	173.0
80	94.0
81	72.5
82	51.0
83	31.0
84	11.0
85	7.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.241266683602998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28758875522901	19.692054831958153
2	10.974906854797	5.9794072891845556
3	4.013136687907525	3.2796878025912046
4	1.9479503182285973	2.1225853642109818
5	1.1506572214758208	1.5672680115818243
6	0.712801612349396	1.165057128870726
7	0.6255968507708592	1.1929435453789816
8	0.4632111744667002	1.009476730757787
9	0.37860185740468727	0.928223474802167
>10	6.6618516864337725	46.32222068180128
>50	0.7101986817430082	11.676939487467038
>100	0.06495198533389725	3.165550406952096
>500	0.005982419701806327	1.034152747494389
>1k	0.002563894157916997	0.8644324969488218
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1524	0.3534995059357299	RNA PCR Primer, Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1174	0.27231523619983394	RNA PCR Primer, Index 24 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	1015	0.23543438223409832	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.2140945170463771	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	697	0.1616726743026271	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	588	0.13638957315630523	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	572	0.13267829225409286	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	566	0.13128656191576318	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	557	0.12919896640826875	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	539	0.1250237753932798	RNA PCR Primer, Index 24 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	435	0.10090044952889927	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.319550563882742E-4	0.0	0.0	0.24355280920768793	0.0
2	2.319550563882742E-4	0.0	0.0	1.1057297538029032	0.0
3	2.319550563882742E-4	0.0	0.0	1.4872958215616143	0.0
4	2.319550563882742E-4	0.0	0.0	1.907598383737167	0.0
5	2.319550563882742E-4	0.0	0.0	3.0928887218812484	0.0
6	2.319550563882742E-4	0.0	0.0	3.728677531441508	0.0
7	2.319550563882742E-4	0.0	0.0	4.364234385945379	0.0
8	2.319550563882742E-4	0.0	0.0	5.325456139618388	0.0
9	2.319550563882742E-4	0.0	0.0	5.72302710626789	0.0
10	2.319550563882742E-4	0.0	0.0	6.634842432930196	0.0
11	2.319550563882742E-4	0.0	0.0	7.925208411618165	0.0
12	2.319550563882742E-4	0.0	0.0	8.880399333825078	0.0
13	2.319550563882742E-4	0.0	0.0	9.321345896019187	0.0
14	2.319550563882742E-4	0.0	0.0	9.519667469231162	0.0
15	2.319550563882742E-4	0.0	0.0	9.914223020147617	0.0
16	2.319550563882742E-4	0.0	0.0	10.615423155609369	0.0
17	4.639101127765484E-4	0.0	0.0	11.449765493437992	0.0
18	4.639101127765484E-4	0.0	0.0	12.336761629066752	0.0
19	4.639101127765484E-4	0.0	0.0	12.875593225056713	0.0
20	4.639101127765484E-4	0.0	0.0	13.394476686197283	0.0
21	6.958651691648226E-4	0.0	0.0	14.05856401263691	0.0
22	6.958651691648226E-4	0.0	0.0	14.720331788512658	0.0
23	6.958651691648226E-4	0.0	0.0	15.364239025046507	0.0
24	9.278202255530968E-4	0.0	0.0	15.880570980566805	0.0
25	9.278202255530968E-4	0.0	0.0	16.373707430448277	0.0
26	9.278202255530968E-4	0.0	0.0	16.815813767924325	0.0
27	9.278202255530968E-4	0.0	0.0	17.270677633501734	0.0
28	9.278202255530968E-4	0.0	0.0	17.73992271257521	0.0
29	9.278202255530968E-4	0.0	0.0	18.203368915238983	0.0
30	9.278202255530968E-4	0.0	0.0	18.703464016812102	0.0
31	9.278202255530968E-4	0.0	0.0	19.17665233184418	0.0
32	9.278202255530968E-4	0.0	0.0	19.657495163737075	0.0
33	9.278202255530968E-4	0.0	0.0	20.096354130423688	0.0
34	9.278202255530968E-4	0.0	0.0	20.56258379376412	0.0
35	9.278202255530968E-4	0.0	0.0	21.041570985205908	0.0
36	9.278202255530968E-4	0.0	0.0	21.511743884504938	0.0
37	9.278202255530968E-4	0.0	0.0	21.971710761322885	0.0
38	9.278202255530968E-4	0.0	0.0	22.436084784212213	0.0
39	9.278202255530968E-4	0.0	0.0	22.92643777341702	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	25	3.8867354E-5	45.000004	2
CGCATGG	25	3.8867354E-5	45.000004	2
GCGTTAC	25	3.8867354E-5	45.000004	24
GTTACGT	25	3.8867354E-5	45.000004	26
GCGAGCA	25	3.8867354E-5	45.000004	20
TATTTCG	20	7.028378E-4	45.0	17
TAATACG	20	7.028378E-4	45.0	1
CACGGGT	90	0.0	45.0	4
ATCAGCG	20	7.028378E-4	45.0	31
CGTTGAT	45	3.8380676E-10	45.0	25
ACGGGCT	55	1.8189894E-12	45.0	5
TATTGCG	20	7.028378E-4	45.0	1
ACGATTT	20	7.028378E-4	45.0	45
ACGGATA	20	7.028378E-4	45.0	45
ACTACCG	20	7.028378E-4	45.0	1
GTACTGC	20	7.028378E-4	45.0	25
CGTAAGG	40	6.7975634E-9	45.0	2
TTACGAG	20	7.028378E-4	45.0	1
GCGTGCC	20	7.028378E-4	45.0	28
TCGACGG	20	7.028378E-4	45.0	2
>>END_MODULE