SRR3551223_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	605928
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	8147	1.3445491873621949	RNA PCR Primer, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG	7180	1.1849592690880766	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	6261	1.0332910840891987	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3022	0.49873912412035754	No Hit
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2365	0.3903103999155015	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCAAAGCT	1708	0.2818816757106455	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	1707	0.2817166396007446	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	1427	0.23550652882850767	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	1328	0.21916795394832392	RNA PCR Primer, Index 13 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTC	1282	0.211576292892885	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTA	1036	0.17097740985727677	No Hit
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	1000	0.1650361099008463	RNA PCR Primer, Index 13 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	738	0.12179664910682457	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAA	45	3.8380676E-10	45.000004	33
GATCCGA	45	3.8380676E-10	45.000004	23
ACCCGTC	45	3.8380676E-10	45.000004	39
CGGGTTC	20	7.0306426E-4	45.0	6
TAGCGGA	20	7.0306426E-4	45.0	3
GCGTTAG	40	6.8066583E-9	45.0	1
TATTGCG	20	7.0306426E-4	45.0	1
GTTACGT	40	6.8066583E-9	45.0	40
GGACGTA	25	3.888614E-5	45.0	8
GCGATAT	20	7.0306426E-4	45.0	9
GCCTACG	25	3.888614E-5	45.0	1
CATCGGA	25	3.888614E-5	45.0	26
GCGTGAT	20	7.0306426E-4	45.0	9
AGTAGCG	20	7.0306426E-4	45.0	1
AATCGTT	25	3.888614E-5	45.0	22
TAATCGT	25	3.888614E-5	45.0	21
CGACAAA	30	2.163797E-6	44.999996	12
ATCCGGC	60	0.0	44.999996	6
GTACGAG	30	2.163797E-6	44.999996	1
CGTTTTT	1840	0.0	43.53261	1