##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	605928
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25794153760843	33.0	31.0	34.0	30.0	34.0
2	32.36778957235843	34.0	31.0	34.0	30.0	34.0
3	32.38488071189976	34.0	31.0	34.0	30.0	34.0
4	35.929504825655854	37.0	35.0	37.0	35.0	37.0
5	35.95277161642967	37.0	35.0	37.0	35.0	37.0
6	35.978041945577694	37.0	35.0	37.0	35.0	37.0
7	36.136925509301435	37.0	35.0	37.0	35.0	37.0
8	35.70315284984355	37.0	35.0	37.0	35.0	37.0
9	37.6534555260691	39.0	38.0	39.0	35.0	39.0
10	37.49807567895856	39.0	37.0	39.0	35.0	39.0
11	37.43101325570035	39.0	37.0	39.0	35.0	39.0
12	37.35889412603478	39.0	37.0	39.0	35.0	39.0
13	37.31848833524775	39.0	37.0	39.0	34.0	39.0
14	38.619715543760975	40.0	38.0	41.0	35.0	41.0
15	38.62382164217531	40.0	38.0	41.0	34.0	41.0
16	38.73983212526901	40.0	38.0	41.0	35.0	41.0
17	38.686045206691226	40.0	38.0	41.0	35.0	41.0
18	38.66998554283677	40.0	38.0	41.0	35.0	41.0
19	38.632377114112565	40.0	38.0	41.0	35.0	41.0
20	38.390036439973066	40.0	38.0	41.0	34.0	41.0
21	38.48691263648486	40.0	38.0	41.0	34.0	41.0
22	38.440692623546035	40.0	38.0	41.0	34.0	41.0
23	38.305128662151276	40.0	37.0	41.0	34.0	41.0
24	38.32195244319457	40.0	37.0	41.0	34.0	41.0
25	38.36457796965976	40.0	37.0	41.0	34.0	41.0
26	38.29372961804043	40.0	37.0	41.0	34.0	41.0
27	38.16063294648869	40.0	37.0	41.0	34.0	41.0
28	38.10796002165274	40.0	37.0	41.0	34.0	41.0
29	38.110582445439064	40.0	37.0	41.0	34.0	41.0
30	38.04912464847309	40.0	37.0	41.0	34.0	41.0
31	37.99279452344173	40.0	37.0	41.0	34.0	41.0
32	37.72491451129507	40.0	37.0	41.0	33.0	41.0
33	37.578005967705735	40.0	37.0	41.0	33.0	41.0
34	37.42998838145786	40.0	37.0	41.0	33.0	41.0
35	37.319838330626744	40.0	37.0	41.0	32.0	41.0
36	37.30171736575963	40.0	37.0	41.0	32.0	41.0
37	37.23058020094797	40.0	36.0	41.0	32.0	41.0
38	37.248489919594405	40.0	36.0	41.0	32.0	41.0
39	37.13637428869437	40.0	36.0	41.0	32.0	41.0
40	37.03741533647562	40.0	36.0	41.0	31.0	41.0
41	37.01894284469442	40.0	36.0	41.0	31.0	41.0
42	36.96353197079521	40.0	36.0	41.0	31.0	41.0
43	36.89321503544976	40.0	36.0	41.0	31.0	41.0
44	36.944962107709166	40.0	36.0	41.0	31.0	41.0
45	36.904132174119695	40.0	35.0	41.0	31.0	41.0
46	36.77828223815371	40.0	35.0	41.0	31.0	41.0
47	36.66092010931992	39.0	35.0	41.0	31.0	41.0
48	36.701855666019725	39.0	35.0	41.0	31.0	41.0
49	36.62089225122457	39.0	35.0	41.0	31.0	41.0
50	36.51197667049551	39.0	35.0	41.0	31.0	41.0
51	35.824012093846136	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	12.0
10	5.0
11	5.0
12	9.0
13	5.0
14	11.0
15	11.0
16	22.0
17	52.0
18	107.0
19	228.0
20	497.0
21	1113.0
22	2184.0
23	3256.0
24	4183.0
25	4920.0
26	5746.0
27	6306.0
28	5768.0
29	5846.0
30	6850.0
31	8632.0
32	11148.0
33	14981.0
34	26291.0
35	40137.0
36	35371.0
37	52647.0
38	101368.0
39	267651.0
40	566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.550388825074926	20.915026207734254	23.515170119222088	12.019414847968736
2	30.970346311773017	30.367469402305225	22.577765014985278	16.084419270936483
3	28.366406569757462	30.260856075309277	25.963315773491242	15.40942158144202
4	25.625816928744012	23.741764698115947	32.913481469745584	17.718936903394464
5	29.360584095800164	26.900885913837946	24.740563235235868	18.997966755126022
6	23.726581376005072	36.957691342865814	24.99801956668119	14.317707714447922
7	74.84717656223182	4.8946409474393	15.379385009440066	4.878797480888818
8	74.95494514199706	9.851335472201319	10.321193277089028	4.872526108712586
9	69.77792741051742	6.876724627348464	11.165683051451659	12.179664910682456
10	38.2408470973449	28.94716864049854	19.290905850200023	13.521078411956536
11	27.795051557280733	23.729552025983285	28.980010826368808	19.495385590367174
12	24.25733750544619	21.003485562641107	31.777372889188154	22.96180404272455
13	23.694069262354603	23.539925535707212	34.57324962701839	18.19275557491979
14	18.2917772408603	30.66502950845645	30.041688121360956	21.001505129322297
15	17.16309528524841	26.34718976512061	36.07095232436857	20.418762625262406
16	19.677585455697706	26.645079943491634	30.242372031000382	23.434962569810274
17	19.641937655959126	28.510978202030607	29.777135237189896	22.069948904820375
18	20.355058686840678	26.842463130933048	30.54224264269022	22.26023553953605
19	20.3004317344635	28.53391822130682	27.995405394700363	23.170244649529316
20	23.899539219181158	27.925760156322205	30.83287123222561	17.34182939227103
21	21.36904054607148	29.970557557993686	30.431173340726954	18.22922855520788
22	19.7157087970848	24.196109108672978	32.33403968788371	23.75414240635851
23	20.96915805178173	27.622093714104643	31.100394766374883	20.30835346773874
24	21.159444686497405	26.780244517500428	29.85816796715121	22.202142828850953
25	18.891683500349878	32.504852061631084	27.212969197660446	21.39049524035859
26	18.26850714936428	27.738114099364942	31.54681744365667	22.446561307614104
27	22.485509829550708	27.586115842146263	28.523355910273168	21.405018418029865
28	17.342654572820535	28.282568225927836	31.57635890732892	22.79841829392271
29	19.7346879497234	24.900813297949593	32.59199112765873	22.77250762466828
30	18.57646453043926	29.55961764434058	29.114515255938002	22.74940256928216
31	19.790635190979785	27.175340964603055	26.300154473798866	26.73386937061829
32	22.421310782799274	30.352121044084445	25.964471026260544	21.262097146855734
33	20.64156137362855	29.280376546388347	25.156949340515705	24.921112739467397
34	19.27638267252875	28.87735836601048	28.13519097978638	23.71106798167439
35	21.92669756142644	26.730073540090572	27.136062370446655	24.207166528036332
36	18.810650770388563	29.509776739150528	26.38679843149681	25.292774058964103
37	18.58768698591252	28.883959810406516	28.306168389643656	24.222184814037313
38	19.105405262671475	28.967798154236146	25.06617948007024	26.86061710302214
39	20.331953631454564	30.889973726251302	26.466345836469017	22.311726805825113
40	21.227604599886455	27.927575553531113	28.696478789559155	22.148341057023277
41	19.042526504799252	28.07412761912306	26.946930988500284	25.936414887577403
42	21.330917204684386	25.30449162276706	29.773174370552276	23.591416801996274
43	21.050355817852946	25.803890891327026	27.12351962609419	26.022233664725842
44	20.23293196551405	26.165485008119777	28.043430902681504	25.558152123684664
45	19.70366116106204	25.84614013546164	27.01641119076854	27.43378751270778
46	22.842152863046433	28.47500033007222	26.218296563288046	22.464550243593298
47	18.49015064496112	26.914913983179517	31.90890666877913	22.686028703080233
48	20.07845816664686	25.981469745580334	28.227116093001147	25.712955994771658
49	19.904014998481667	24.468253653899474	31.961058079507797	23.66667326811106
50	19.698214969435313	24.669927780198307	29.61407956060786	26.01777768975852
51	18.843162884039028	24.447789176271765	27.78597457123619	28.923073368453018
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	406.0
2	641.0
3	2892.5
4	5144.0
5	3557.5
6	1971.0
7	1898.0
8	1825.0
9	1844.5
10	1864.0
11	1811.5
12	1759.0
13	1705.5
14	1652.0
15	1609.5
16	1567.0
17	1549.0
18	1531.0
19	1607.0
20	1683.0
21	2032.0
22	2381.0
23	2653.0
24	2925.0
25	3707.0
26	5255.5
27	6022.0
28	7266.5
29	8511.0
30	10039.0
31	11567.0
32	12974.5
33	14382.0
34	16962.0
35	19542.0
36	19852.0
37	20162.0
38	22245.0
39	24328.0
40	27508.5
41	30689.0
42	33300.5
43	35912.0
44	39546.0
45	43180.0
46	61032.5
47	78885.0
48	71093.5
49	63302.0
50	60567.5
51	57833.0
52	50180.5
53	42528.0
54	36488.5
55	30449.0
56	26545.0
57	22641.0
58	20822.0
59	19003.0
60	17200.5
61	15398.0
62	13702.5
63	12007.0
64	9695.5
65	7384.0
66	6154.5
67	4925.0
68	4197.0
69	3469.0
70	2611.0
71	1753.0
72	1528.5
73	1304.0
74	950.0
75	427.5
76	259.0
77	218.0
78	177.0
79	115.5
80	54.0
81	41.5
82	29.0
83	17.0
84	5.0
85	9.0
86	13.0
87	11.5
88	10.0
89	6.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	605928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.41412881193344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65254091264688	24.610354893813806
2	10.098089544606223	6.748377295958212
3	3.6677088842133174	3.6765989130532937
4	1.9622693709079813	2.622700860929235
5	1.3267017244133772	2.21652911572814
6	0.9640092870735395	1.9326918296505218
7	0.7712268597462707	1.8038911544349372
8	0.6688972923090184	1.7880496229733627
9	0.5966327388569831	1.7942366870625492
>10	6.129331138508823	41.31336563009995
>50	0.11271732441168905	2.360635606580521
>100	0.04189493473697411	2.649444749264541
>500	0.002493746115296078	0.5731186622669995
>1k	0.0039899937844737245	2.312306321301139
>5k	0.0014962476691776467	3.5976986568828098
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	8147	1.3445491873621949	RNA PCR Primer, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG	7180	1.1849592690880766	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	6261	1.0332910840891987	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3022	0.49873912412035754	No Hit
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2365	0.3903103999155015	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCAAAGCT	1708	0.2818816757106455	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	1707	0.2817166396007446	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	1427	0.23550652882850767	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	1328	0.21916795394832392	RNA PCR Primer, Index 13 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTC	1282	0.211576292892885	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTA	1036	0.17097740985727677	No Hit
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	1000	0.1650361099008463	RNA PCR Primer, Index 13 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCC	738	0.12179664910682457	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6503610990084631E-4	0.0	0.0	0.16916201264836747	0.0
2	1.6503610990084631E-4	0.0	0.0	0.9649661345902484	0.0
3	1.6503610990084631E-4	0.0	0.0	1.3716151093859337	0.0
4	1.6503610990084631E-4	0.0	0.0	2.075824190332845	0.0
5	1.6503610990084631E-4	0.0	0.0	4.718382382065196	0.0
6	1.6503610990084631E-4	0.0	0.0	5.6609036057089295	0.0
7	1.6503610990084631E-4	0.0	0.0	6.678021151027845	0.0
8	1.6503610990084631E-4	0.0	0.0	7.957876183308908	0.0
9	1.6503610990084631E-4	0.0	0.0	8.407104474459011	0.0
10	1.6503610990084631E-4	0.0	0.0	10.586571341809588	0.0
11	3.3007221980169263E-4	0.0	0.0	12.08080828085185	0.0
12	3.3007221980169263E-4	0.0	0.0	14.302854464556845	0.0
13	3.3007221980169263E-4	0.0	0.0	14.867608032637541	0.0
14	3.3007221980169263E-4	0.0	0.0	15.11120133085119	0.0
15	3.3007221980169263E-4	0.0	0.0	15.616376863257681	0.0
16	4.951083297025389E-4	0.0	0.0	16.33015803857884	0.0
17	4.951083297025389E-4	0.0	0.0	17.18818077395334	0.0
18	4.951083297025389E-4	0.0	0.0	18.089773042341665	0.0
19	4.951083297025389E-4	0.0	0.0	18.965949749805258	0.0
20	4.951083297025389E-4	0.0	0.0	19.554798589931476	0.0
21	4.951083297025389E-4	0.0	0.0	20.21296259621605	0.0
22	4.951083297025389E-4	0.0	0.0	21.014377945894562	0.0
23	4.951083297025389E-4	0.0	0.0	21.709179968577125	0.0
24	4.951083297025389E-4	0.0	0.0	22.25825510621724	0.0
25	4.951083297025389E-4	0.0	0.0	22.750557822051466	0.0
26	4.951083297025389E-4	0.0	0.0	23.19995114931147	0.0
27	4.951083297025389E-4	0.0	0.0	23.711728126113993	0.0
28	4.951083297025389E-4	0.0	0.0	24.203535733618516	0.0
29	4.951083297025389E-4	0.0	0.0	24.68214045233097	0.0
30	4.951083297025389E-4	0.0	0.0	25.233030987179994	0.0
31	4.951083297025389E-4	0.0	0.0	25.744312855652815	0.0
32	4.951083297025389E-4	0.0	0.0	26.251303785268217	0.0
33	4.951083297025389E-4	0.0	0.0	26.72974346787077	0.0
34	4.951083297025389E-4	0.0	0.0	27.21726013651787	0.0
35	4.951083297025389E-4	0.0	0.0	27.70444673294517	0.0
36	4.951083297025389E-4	0.0	0.0	28.18816757106455	0.0
37	4.951083297025389E-4	0.0	0.0	28.67766467303046	0.0
38	4.951083297025389E-4	0.0	0.0	29.158909969501327	0.0
39	4.951083297025389E-4	0.0	0.0	29.65484347975337	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAA	45	3.8380676E-10	45.000004	33
GATCCGA	45	3.8380676E-10	45.000004	23
ACCCGTC	45	3.8380676E-10	45.000004	39
CGGGTTC	20	7.0306426E-4	45.0	6
TAGCGGA	20	7.0306426E-4	45.0	3
GCGTTAG	40	6.8066583E-9	45.0	1
TATTGCG	20	7.0306426E-4	45.0	1
GTTACGT	40	6.8066583E-9	45.0	40
GGACGTA	25	3.888614E-5	45.0	8
GCGATAT	20	7.0306426E-4	45.0	9
GCCTACG	25	3.888614E-5	45.0	1
CATCGGA	25	3.888614E-5	45.0	26
GCGTGAT	20	7.0306426E-4	45.0	9
AGTAGCG	20	7.0306426E-4	45.0	1
AATCGTT	25	3.888614E-5	45.0	22
TAATCGT	25	3.888614E-5	45.0	21
CGACAAA	30	2.163797E-6	44.999996	12
ATCCGGC	60	0.0	44.999996	6
GTACGAG	30	2.163797E-6	44.999996	1
CGTTTTT	1840	0.0	43.53261	1
>>END_MODULE