##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551222_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	536123
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24272601623135	33.0	31.0	34.0	30.0	34.0
2	32.35552662355467	34.0	31.0	34.0	30.0	34.0
3	32.3945773637766	34.0	31.0	34.0	30.0	34.0
4	35.903223700531406	37.0	35.0	37.0	35.0	37.0
5	35.96530646885137	37.0	35.0	37.0	35.0	37.0
6	36.019295945146915	37.0	35.0	37.0	35.0	37.0
7	36.24267192416666	37.0	37.0	37.0	35.0	37.0
8	35.981599371785954	37.0	36.0	37.0	35.0	37.0
9	37.864751185828624	39.0	38.0	39.0	35.0	39.0
10	37.64639271212017	39.0	37.0	39.0	35.0	39.0
11	37.57529895191961	39.0	37.0	39.0	35.0	39.0
12	37.546048201625375	39.0	37.0	39.0	35.0	39.0
13	37.52311689668229	39.0	37.0	39.0	35.0	39.0
14	38.77843517252571	40.0	38.0	41.0	35.0	41.0
15	38.8392383837291	40.0	38.0	41.0	35.0	41.0
16	38.90765738459272	40.0	38.0	41.0	35.0	41.0
17	38.922956112683096	40.0	38.0	41.0	35.0	41.0
18	38.94683123089291	40.0	38.0	41.0	35.0	41.0
19	38.93026040666041	40.0	38.0	41.0	35.0	41.0
20	38.872607591914544	40.0	38.0	41.0	35.0	41.0
21	38.845966317430886	40.0	38.0	41.0	35.0	41.0
22	38.83090447527899	40.0	38.0	41.0	35.0	41.0
23	38.78388354911093	40.0	38.0	41.0	35.0	41.0
24	38.74965446362122	40.0	38.0	41.0	35.0	41.0
25	38.68320702525353	40.0	38.0	41.0	35.0	41.0
26	38.697496656550825	40.0	38.0	41.0	35.0	41.0
27	38.66476722692367	40.0	38.0	41.0	35.0	41.0
28	38.632914461793284	40.0	38.0	41.0	35.0	41.0
29	38.62667149143014	40.0	38.0	41.0	35.0	41.0
30	38.58178253870847	40.0	38.0	41.0	35.0	41.0
31	38.53348392066746	40.0	38.0	41.0	35.0	41.0
32	38.48445599237488	40.0	38.0	41.0	35.0	41.0
33	38.445733535028346	40.0	38.0	41.0	35.0	41.0
34	38.41753478213022	40.0	38.0	41.0	35.0	41.0
35	38.38464307630898	40.0	38.0	41.0	35.0	41.0
36	38.34039017165837	40.0	38.0	41.0	35.0	41.0
37	38.29374788994316	40.0	38.0	41.0	34.0	41.0
38	38.244802032369435	40.0	38.0	41.0	34.0	41.0
39	38.21372334333726	40.0	38.0	41.0	34.0	41.0
40	38.137604616850986	40.0	38.0	41.0	34.0	41.0
41	38.119340151420474	40.0	38.0	41.0	34.0	41.0
42	38.09016774135786	40.0	37.0	41.0	34.0	41.0
43	38.00992682649317	40.0	37.0	41.0	34.0	41.0
44	37.98262525577153	40.0	37.0	41.0	34.0	41.0
45	37.9333660372713	40.0	37.0	41.0	34.0	41.0
46	37.81584822885793	40.0	37.0	41.0	33.0	41.0
47	37.77302037032547	40.0	37.0	41.0	33.0	41.0
48	37.78736409368746	40.0	37.0	41.0	33.0	41.0
49	37.715636150659456	40.0	37.0	41.0	33.0	41.0
50	37.690968303915334	40.0	36.0	41.0	33.0	41.0
51	36.9256420634817	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	5.0
12	4.0
13	8.0
14	5.0
15	12.0
16	17.0
17	39.0
18	54.0
19	97.0
20	219.0
21	348.0
22	542.0
23	768.0
24	1151.0
25	1716.0
26	2361.0
27	2901.0
28	3301.0
29	3800.0
30	4823.0
31	6320.0
32	8590.0
33	11857.0
34	21056.0
35	34482.0
36	29883.0
37	46821.0
38	95660.0
39	258790.0
40	486.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.40018615131229	22.83916190874109	26.852793109044004	12.90785883090261
2	28.386396405302516	25.40070095854869	27.843050941668235	18.36985169448056
3	31.775544044929987	24.54996334796306	27.79138369366731	15.883108913439639
4	27.264825422524307	27.252514814697378	27.230877988819728	18.25178177395859
5	23.962784659490453	30.144575032222082	26.192496871053844	19.70014343723362
6	24.38806020260276	32.54421093666938	26.254982531993594	16.812746328734267
7	81.57605624082534	4.367840961868825	8.88732622924217	5.168776568063672
8	80.53860774486452	5.020489701057407	8.475293915761869	5.9656086383162075
9	74.54968356142155	7.491004862690091	11.98736110929768	5.971950466590689
10	32.513993990185085	41.30022401575758	16.113279974931125	10.072502019126208
11	21.185623448350473	24.90249439027984	33.13717188033343	20.774710281036253
12	22.718294122803908	21.97760588521664	35.49782419332877	19.80627579865068
13	21.671519408792385	25.211192207758295	35.22326033391591	17.894028049533407
14	17.235410530792375	29.50442342522145	31.119724391604166	22.14044165238201
15	15.186813473773741	29.3615457646846	36.811888316673596	18.639752444868062
16	17.914359204883954	29.51412269199419	32.09170283684901	20.47981526627285
17	17.677659790756973	28.0873978545968	30.38854889642862	23.84639345821761
18	18.699813289114626	26.850927865433867	33.289002710198965	21.160256135252546
19	19.043018113380697	28.090382244373025	31.33758484526872	21.52901479697756
20	20.71670120476085	29.18099018322288	31.578201270976997	18.524107341039276
21	20.712038095735494	27.61679689175805	32.12080063716722	19.550364375339242
22	19.122477491172734	25.78699291020904	30.894216439138035	24.196313159480194
23	17.39041227479515	28.5757186317319	31.35679685445318	22.677072239019775
24	19.606694732365522	25.794826933371635	31.35064155053971	23.247836783723137
25	17.53496865458113	30.638118491465576	28.594744116555344	23.23216873739795
26	17.70395972565997	30.775586945533025	28.66898081223898	22.851472516568027
27	18.976055867776612	29.564484269468018	29.90638342320699	21.553076439548388
28	15.407098744131478	28.051398652921065	33.443817929840726	23.09768467310673
29	17.22701693454674	26.605461806339214	30.842735715498122	25.324785543615924
30	18.71883877393807	28.143914735984094	29.617830236718067	23.519416253359772
31	18.722755785519368	28.7508650067242	27.536777940882967	24.98960126687346
32	17.783978676535046	30.843295288581164	25.52492618298413	25.847799851899655
33	17.62207553117475	29.71818034294369	26.756733063121708	25.90301106275985
34	17.000762884636547	27.251022619809262	29.024496244331992	26.723718251222202
35	15.442911421446198	27.65895139734725	26.556592423753507	30.341544757453047
36	17.55324804196052	30.653413489068736	27.533980075467756	24.259358393502982
37	16.154128809993228	26.515370539969375	30.810093952320646	26.52040669771675
38	15.934776161440563	28.86203352588865	28.18756143646141	27.01562887620938
39	18.304754692486615	28.056434810668446	28.316636294283214	25.322174202561726
40	19.3194472164037	26.660299968477386	28.667302092989853	25.352950722129066
41	16.911977288793803	27.9202720271281	26.23819533950232	28.929555344575782
42	19.147098706826604	26.718868617835835	28.26832648478054	25.86570619055702
43	20.109191360937693	27.100124411748794	28.56564631623713	24.225037911076377
44	18.31949011700673	26.167875655399975	29.2826459599756	26.229988267617692
45	18.91916593766729	25.011797665834145	27.304555111420324	28.764481285078237
46	21.093666938370486	25.77319010749399	27.77888656147936	25.35425639265616
47	16.02524047653244	25.936398923381386	31.539590728247063	26.498769871839112
48	17.78547087142316	25.106552041229346	29.346250767081433	27.761726320266057
49	18.304381643764582	23.544783566457696	33.00847007123365	25.142364718544062
50	18.56626184662848	23.60838837356353	29.888104035827602	27.937245743980395
51	16.789617307968506	22.970102010173036	27.734866812280018	32.50541386957843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	181.0
1	376.0
2	571.0
3	1291.0
4	2011.0
5	1387.0
6	763.0
7	761.5
8	760.0
9	814.5
10	869.0
11	873.5
12	878.0
13	881.5
14	885.0
15	898.0
16	911.0
17	986.5
18	1062.0
19	1316.0
20	1570.0
21	1807.0
22	2044.0
23	2493.0
24	2942.0
25	3236.5
26	4665.0
27	5799.0
28	7102.5
29	8406.0
30	10109.5
31	11813.0
32	13461.5
33	15110.0
34	16373.0
35	17636.0
36	19087.5
37	20539.0
38	22438.5
39	24338.0
40	26793.0
41	29248.0
42	32947.5
43	36647.0
44	42309.5
45	47972.0
46	54955.5
47	61939.0
48	66882.0
49	71825.0
50	66165.5
51	60506.0
52	48897.5
53	37289.0
54	29694.0
55	22099.0
56	18410.0
57	14721.0
58	12833.0
59	10945.0
60	9700.0
61	8455.0
62	7126.0
63	5797.0
64	4415.5
65	3034.0
66	2243.5
67	1453.0
68	1113.0
69	773.0
70	544.5
71	316.0
72	310.5
73	305.0
74	193.5
75	77.5
76	73.0
77	45.5
78	18.0
79	12.0
80	6.0
81	3.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	536123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.30255418380918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.17767118248926	21.341352463157957
2	13.020969618229076	8.151792140006973
3	5.1468664224122564	4.833301951931633
4	2.646577292126678	3.3137851635373723
5	1.5608935379782922	2.442997727386155
6	1.0474695998965406	1.9673084343992626
7	0.7810865454441185	1.711500273770619
8	0.6030810323390767	1.5102381353617218
9	0.521920577999278	1.4703702455220664
>10	6.24915334396573	41.98361636385833
>50	0.16969374110279803	3.418042028303249
>100	0.06498909233724176	3.723190715939711
>500	0.004814006839795686	0.9872125135201875
>1k	0.004814006839795686	3.1452918433047303
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	3381	0.6306388645889096	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	3346	0.6241105119534136	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	2641	0.49261083743842365	No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	1933	0.3605515898403911	No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	1707	0.3183970842511886	No Hit
CTGAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	1527	0.284822699268638	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1134	0.21151862539006908	No Hit
ACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	1029	0.1919335674835812	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	969	0.18074210582273098	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	800	0.1492194888113362	No Hit
CTGAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTAT	651	0.12142735902022483	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	611	0.11396638457965803	No Hit
ACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	588	0.10967632427633212	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	572	0.10669193450010539	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.595730830425108E-4	0.0	0.0	0.3683856130029863	0.0
2	5.595730830425108E-4	0.0	0.0	2.040576509495023	0.0
3	5.595730830425108E-4	0.0	0.0	2.7344471324677357	0.0
4	5.595730830425108E-4	0.0	0.0	3.714632649597201	0.0
5	5.595730830425108E-4	0.0	0.0	6.1379571478933	0.0
6	5.595730830425108E-4	0.0	0.0	7.3076514158131625	0.0
7	5.595730830425108E-4	0.0	0.0	8.91101482309097	0.0
8	5.595730830425108E-4	0.0	0.0	10.837811472367349	0.0
9	5.595730830425108E-4	0.0	0.0	11.525153742704566	0.0
10	5.595730830425108E-4	0.0	0.0	13.185966653174738	0.0
11	5.595730830425108E-4	0.0	0.0	15.538971467368496	0.0
12	5.595730830425108E-4	0.0	0.0	18.236486776355427	0.0
13	5.595730830425108E-4	0.0	0.0	18.97922678191385	0.0
14	5.595730830425108E-4	0.0	0.0	19.36738397718434	0.0
15	5.595730830425108E-4	0.0	0.0	19.855145181236395	0.0
16	5.595730830425108E-4	0.0	0.0	20.832346308589635	0.0
17	5.595730830425108E-4	0.0	0.0	22.395047405166352	0.0
18	5.595730830425108E-4	0.0	0.0	23.87325296620365	0.0
19	5.595730830425108E-4	0.0	0.0	24.852505861528044	0.0
20	5.595730830425108E-4	0.0	0.0	25.754164622670544	0.0
21	7.46097444056681E-4	0.0	0.0	26.891217127412926	0.0
22	7.46097444056681E-4	0.0	0.0	28.076765966018993	0.0
23	7.46097444056681E-4	0.0	0.0	29.184720670443163	0.0
24	7.46097444056681E-4	0.0	0.0	29.974651339338173	0.0
25	7.46097444056681E-4	0.0	0.0	30.668895011032916	0.0
26	7.46097444056681E-4	0.0	0.0	31.314642348863973	0.0
27	7.46097444056681E-4	0.0	0.0	31.99974632686902	0.0
28	7.46097444056681E-4	0.0	0.0	32.69137865750957	0.0
29	7.46097444056681E-4	0.0	0.0	33.390471962590674	0.0
30	7.46097444056681E-4	0.0	0.0	34.159325378691086	0.0
31	9.326218050708513E-4	0.0	0.0	34.84871941699946	0.0
32	9.326218050708513E-4	0.0	0.0	35.479917854671406	0.0
33	9.326218050708513E-4	0.0	0.0	36.07828800480487	0.0
34	0.0011191461660850215	0.0	0.0	36.717880038722456	0.0
35	0.0011191461660850215	0.0	0.0	37.424061269522106	0.0
36	0.0011191461660850215	0.0	0.0	38.068502936826064	0.0
37	0.0011191461660850215	0.0	0.0	38.71555594518422	0.0
38	0.0011191461660850215	0.0	0.0	39.303853779822916	0.0
39	0.0011191461660850215	0.0	0.0	39.91584020831041	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	20	7.0299144E-4	45.000004	6
GATCGGA	20	7.0299144E-4	45.000004	9
GTCGACG	20	7.0299144E-4	45.000004	1
CCCTATA	20	7.0299144E-4	45.000004	37
ATTACCG	20	7.0299144E-4	45.000004	1
GTAGGCG	40	6.8030204E-9	45.000004	1
TCGGCGT	20	7.0299144E-4	45.000004	4
CCTCCGG	20	7.0299144E-4	45.000004	2
ACGGCGC	20	7.0299144E-4	45.000004	10
ACGGAAT	20	7.0299144E-4	45.000004	5
CGGGCCG	20	7.0299144E-4	45.000004	6
CCAAGCG	20	7.0299144E-4	45.000004	20
TAGATCG	20	7.0299144E-4	45.000004	28
GCGTCGC	20	7.0299144E-4	45.000004	14
CGCTTCT	20	7.0299144E-4	45.000004	18
CGCCAGG	40	6.8030204E-9	45.000004	2
TGCAACG	20	7.0299144E-4	45.000004	1
GCGAGAC	35	1.210301E-7	45.0	21
CGGCGCT	25	3.8880113E-5	45.0	11
TTTACGG	45	3.8380676E-10	45.0	2
>>END_MODULE