FastQCFastQC Report
Sat 18 Jun 2016
SRR3551220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006288
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84340.8381298395687914No Hit
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC21820.21683653188749147TruSeq Adapter, Index 13 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT17310.17201834862385323No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT14510.14419331245130618TruSeq Adapter, Index 19 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC11390.11318827214475378No Hit
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC10840.10772264003943205TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG600.045.0000041
AACCGGT207.032867E-445.036
ATTCGCG253.890458E-545.01
CACGATA406.8139343E-945.034
TAATGCG207.032867E-445.01
CGTTTTT43550.042.3650971
TCCCGAT603.6379788E-1241.25000444
CGAATAT2300.041.0869614
TAGCGAG556.002665E-1140.9090921
AGCTGCG501.0804797E-940.51
CATACGG950.040.263162
ATTAGCG451.9279469E-840.01
TATAGCG451.9279469E-840.01
ACGGGCC850.039.7058835
TTTCGCG403.4576442E-739.3751
CGTTGAT800.039.37525
TGCGTTG800.039.3751
CGACGGT356.2475156E-638.57142628
ATATGCG356.2475156E-638.5714261
CTACGAA2400.038.43750411
Produced by FastQC (version 0.11.5)