##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551220_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1006288
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.386556333773235	33.0	31.0	34.0	30.0	34.0
2	32.50043923807101	34.0	31.0	34.0	31.0	34.0
3	32.49707737745059	34.0	31.0	34.0	31.0	34.0
4	35.99948126182564	37.0	35.0	37.0	35.0	37.0
5	36.04946397055316	37.0	35.0	37.0	35.0	37.0
6	36.089254766031196	37.0	36.0	37.0	35.0	37.0
7	36.25269008474711	37.0	37.0	37.0	35.0	37.0
8	35.985812212805875	37.0	37.0	37.0	35.0	37.0
9	37.86834087259314	39.0	38.0	39.0	35.0	39.0
10	37.70382932122812	39.0	37.0	39.0	35.0	39.0
11	37.63608628941218	39.0	37.0	39.0	35.0	39.0
12	37.57031486015932	39.0	37.0	39.0	35.0	39.0
13	37.55636060451879	39.0	37.0	39.0	35.0	39.0
14	38.849435748016475	40.0	38.0	41.0	35.0	41.0
15	38.91457117644253	40.0	38.0	41.0	35.0	41.0
16	38.96187274418457	40.0	38.0	41.0	35.0	41.0
17	38.96247396371615	40.0	38.0	41.0	35.0	41.0
18	38.896316958962046	40.0	38.0	41.0	35.0	41.0
19	38.84627959391347	40.0	38.0	41.0	35.0	41.0
20	38.73934897365366	40.0	38.0	41.0	35.0	41.0
21	38.68421962698552	40.0	38.0	41.0	35.0	41.0
22	38.679920658896854	40.0	38.0	41.0	35.0	41.0
23	38.6458449270984	40.0	38.0	41.0	35.0	41.0
24	38.602540227052295	40.0	38.0	41.0	35.0	41.0
25	38.52161110934444	40.0	38.0	41.0	35.0	41.0
26	38.50797684161989	40.0	38.0	41.0	35.0	41.0
27	38.48234799580239	40.0	38.0	41.0	35.0	41.0
28	38.43627172340324	40.0	37.0	41.0	35.0	41.0
29	38.39824682397087	40.0	37.0	41.0	35.0	41.0
30	38.30840872593134	40.0	37.0	41.0	34.0	41.0
31	38.229266373046286	40.0	37.0	41.0	34.0	41.0
32	38.08523901706072	40.0	37.0	41.0	34.0	41.0
33	37.92721864913425	40.0	37.0	41.0	34.0	41.0
34	37.78756479258423	40.0	37.0	41.0	33.0	41.0
35	37.674323851621004	40.0	37.0	41.0	33.0	41.0
36	37.59565353059959	40.0	37.0	41.0	33.0	41.0
37	37.54788489975037	40.0	37.0	41.0	33.0	41.0
38	37.45452693463501	40.0	37.0	41.0	33.0	41.0
39	37.39281994816593	40.0	36.0	41.0	33.0	41.0
40	37.31870697056906	40.0	36.0	41.0	33.0	41.0
41	37.260839839091794	40.0	36.0	41.0	33.0	41.0
42	37.23419537945399	40.0	36.0	41.0	33.0	41.0
43	37.16342935620816	40.0	36.0	41.0	33.0	41.0
44	37.09584631835021	40.0	35.0	41.0	32.0	41.0
45	37.03125546563211	40.0	35.0	41.0	32.0	41.0
46	36.91415777590511	40.0	35.0	41.0	32.0	41.0
47	36.881436527117486	40.0	35.0	41.0	31.0	41.0
48	36.838640627732815	39.0	35.0	41.0	31.0	41.0
49	36.75034980045474	39.0	35.0	41.0	31.0	41.0
50	36.68724460591799	39.0	35.0	41.0	31.0	41.0
51	35.94894503362855	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	9.0
10	18.0
11	19.0
12	20.0
13	21.0
14	16.0
15	34.0
16	61.0
17	114.0
18	166.0
19	308.0
20	487.0
21	864.0
22	1241.0
23	2064.0
24	3336.0
25	5881.0
26	8978.0
27	10494.0
28	10595.0
29	10251.0
30	10976.0
31	13199.0
32	16771.0
33	23072.0
34	43248.0
35	64971.0
36	59163.0
37	89421.0
38	174872.0
39	454505.0
40	1108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.374524986882484	21.841063393382413	24.566426311354206	13.2179853083809
2	34.35875216637781	24.349689154595904	24.533235018205524	16.75832366082076
3	30.823680695784905	23.85370788482025	28.558722751339577	16.76388866805527
4	28.322508069260486	25.113685147790694	28.103584659660058	18.460222123288762
5	25.62347956052343	28.77466490706438	26.43477811521155	19.16707741720064
6	25.365799850539805	33.747197621356904	25.45215683780389	15.434845690299397
7	78.99458206795669	5.829444453277789	10.633436948468034	4.542536530297489
8	80.1187135293276	5.38325012322516	9.98799548439413	4.510040863053122
9	73.5527006185108	8.479679773583705	12.161428934857616	5.806190673047875
10	35.75248835323486	33.0719436185267	18.307880050244066	12.867687977994372
11	26.19727155645302	25.950523110680045	29.475756443483377	18.376448889383557
12	25.73080470004611	23.93290986278282	31.241254988631482	19.095030448539582
13	23.49665304564896	24.173596425675353	32.58967611657895	19.740074412096735
14	19.64497241346414	27.93683319288315	30.856474488416836	21.561719905235876
15	19.096123574960647	28.426653204649167	33.201528786987424	19.275694433402762
16	22.400346620450605	28.013550792616034	30.954855866312624	18.631246720620737
17	22.040409902532872	27.997153896300063	29.312284355969666	20.6501518451974
18	21.88379469893311	27.81748366272876	30.566000985801285	19.732720652536848
19	22.606748763773393	28.916075715898433	28.832898732768353	19.644276787559825
20	23.856987264083443	28.175035377545992	29.504873356335366	18.4631040020352
21	23.432357337064538	27.499185123940663	30.546225335092934	18.522232203901865
22	23.22227831396181	24.66669581670456	30.147731067050387	21.963294802283244
23	21.274823907271077	26.79560920929197	30.913217687182993	21.016349196253955
24	21.148518118073554	26.795509834162782	31.09358354665861	20.96238850110505
25	21.021914203488464	28.845916874691934	28.74644237037508	21.385726551444517
26	20.21787003323104	29.44932265911946	29.067324662522058	21.265482645127438
27	21.339020240726313	28.02766206096068	30.149718569634143	20.48359912867887
28	19.875622088308713	28.081324630722023	30.86959200546961	21.17346127549966
29	20.58108613041197	27.75716295931185	30.22434929165408	21.437401618622104
30	21.5026910784984	28.03531394590813	30.00135150175695	20.460643473836516
31	22.940351072456394	27.796118009953414	27.757262334441034	21.506268583149158
32	23.15231822301369	28.48965703655415	28.0984171529423	20.259607587489864
33	22.72232203901865	27.836066811886855	27.958795096433626	21.48281605266087
34	21.198305057796578	28.25811298554688	27.875319987915987	22.66826196874056
35	21.480927925206302	27.37774871607333	28.827631850921403	22.31369150779896
36	22.906563528532587	28.905641327333726	27.852761833590385	20.335033310543306
37	23.255568982239677	27.646359690267598	28.454180115434152	20.643891212058577
38	22.033652393748113	28.3506312308206	28.336519962475954	21.279196412955336
39	23.129362568171338	26.697426581654554	27.926995055093574	22.246215795080534
40	22.258438935970617	26.398704943316424	29.668643569236643	21.674212551476316
41	20.085502361153072	26.471050037365046	29.802303117994057	23.641144483487828
42	21.926625379612993	26.61027459335697	29.380356319463214	22.08274370756682
43	23.251792727330546	25.890003657004755	28.75151050196365	22.10669311370105
44	22.304052120267755	26.854538660900257	27.81321053217369	23.028198686658293
45	22.607941265323646	26.52997948897334	27.003203854165008	23.85887539153801
46	22.216602006582608	27.096715850730607	27.974198241457714	22.71248390122907
47	21.42875598238278	26.002098802728447	30.43860207018269	22.13054314470609
48	21.580402429523158	25.3481110775444	30.326904424975755	22.74458206795669
49	22.324026521234476	25.21981778576312	29.73403240424213	22.722123288760276
50	20.877025265132847	25.165857090614217	30.564510358863465	23.39260728538947
51	20.964475378817994	24.578748827373474	28.734219229484996	25.722556564323533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	363.0
1	751.5
2	1140.0
3	7023.5
4	12907.0
5	8507.5
6	4108.0
7	4024.5
8	3941.0
9	3986.5
10	4032.0
11	3945.5
12	3859.0
13	3853.5
14	3848.0
15	3716.5
16	3585.0
17	3498.5
18	3412.0
19	3464.5
20	3517.0
21	3788.0
22	4059.0
23	4533.0
24	5007.0
25	5875.0
26	8603.5
27	10464.0
28	11538.0
29	12612.0
30	15471.0
31	18330.0
32	20841.5
33	23353.0
34	27282.0
35	31211.0
36	33611.5
37	36012.0
38	39006.5
39	42001.0
40	46221.0
41	50441.0
42	55978.0
43	61515.0
44	68048.0
45	74581.0
46	83383.0
47	92185.0
48	96735.5
49	101286.0
50	96772.5
51	92259.0
52	80739.0
53	69219.0
54	59847.5
55	50476.0
56	45919.0
57	41362.0
58	38221.0
59	35080.0
60	32225.5
61	29371.0
62	27015.5
63	24660.0
64	21499.5
65	18339.0
66	14857.5
67	11376.0
68	9641.5
69	7907.0
70	6651.5
71	5396.0
72	4193.0
73	2990.0
74	2338.0
75	1319.5
76	953.0
77	726.0
78	499.0
79	316.0
80	133.0
81	78.5
82	24.0
83	29.0
84	34.0
85	21.0
86	8.0
87	5.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1006288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.019001767133652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.13109163717537	21.953223690887803
2	9.846770599907815	5.911804480783848
3	3.685803546118628	3.3193242949272768
4	1.9449949903474726	2.3354723220922753
5	1.2434268764537484	1.8663216800783275
6	0.929258060005084	1.6737239607249483
7	0.7507164584035065	1.5775031084001825
8	0.6200496019438538	1.4890616077170453
9	0.5361071216526923	1.4484060569038595
>10	6.892977354288986	46.23368261798727
>50	0.3593585222265955	6.591414732375009
>100	0.052056558637409954	2.812229755627166
>500	0.005373580246437556	1.1726193549753672
>1k	0.0016792438270117362	0.7649095560311332
>5k	3.3584876540234727E-4	0.8503027804885432
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8434	0.8381298395687914	No Hit
GCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	2182	0.21683653188749147	TruSeq Adapter, Index 13 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1731	0.17201834862385323	No Hit
CTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGCT	1451	0.14419331245130618	TruSeq Adapter, Index 19 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCC	1139	0.11318827214475378	No Hit
CCTGTCTCTTATACACATCTGACGCATGGACTGTCGTATGCCGTCTTCTGC	1084	0.10772264003943205	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.937512918766794E-5	0.0	0.0	0.1484664430063759	0.0
2	9.937512918766794E-5	0.0	0.0	0.6314295708584421	0.0
3	9.937512918766794E-5	0.0	0.0	0.9351199656559553	0.0
4	9.937512918766794E-5	0.0	0.0	1.3713767827898176	0.0
5	9.937512918766794E-5	0.0	0.0	2.5351589207065968	0.0
6	9.937512918766794E-5	0.0	0.0	3.5013833017982923	0.0
7	9.937512918766794E-5	0.0	0.0	4.2733293053280965	0.0
8	9.937512918766794E-5	0.0	0.0	5.3712257325934525	0.0
9	9.937512918766794E-5	0.0	0.0	5.938260219738286	0.0
10	9.937512918766794E-5	0.0	0.0	6.9889534606394985	0.0
11	9.937512918766794E-5	0.0	0.0	8.281724516241871	0.0
12	9.937512918766794E-5	0.0	0.0	9.27756268583149	0.0
13	9.937512918766794E-5	0.0	0.0	9.708651996247594	0.0
14	9.937512918766794E-5	0.0	0.0	9.922507274259457	0.0
15	1.987502583753359E-4	0.0	0.0	10.236234557104924	0.0
16	1.987502583753359E-4	0.0	0.0	10.807542174804826	0.0
17	1.987502583753359E-4	0.0	0.0	11.52880686244892	0.0
18	1.987502583753359E-4	0.0	0.0	12.28445534479195	0.0
19	1.987502583753359E-4	0.0	0.0	12.79613788497925	0.0
20	1.987502583753359E-4	0.0	0.0	13.323621070707391	0.0
21	1.987502583753359E-4	0.0	0.0	13.954653141049084	0.0
22	1.987502583753359E-4	0.0	0.0	14.623149635094526	0.0
23	1.987502583753359E-4	0.0	0.0	15.270976102268932	0.0
24	1.987502583753359E-4	0.0	0.0	15.790012402016123	0.0
25	1.987502583753359E-4	0.0	0.0	16.254491755839283	0.0
26	2.981253875630038E-4	0.0	0.0	16.685084190609448	0.0
27	2.981253875630038E-4	0.0	0.0	17.098186602642585	0.0
28	2.981253875630038E-4	0.0	0.0	17.55342406945129	0.0
29	2.981253875630038E-4	0.0	0.0	18.047914712289128	0.0
30	2.981253875630038E-4	0.0	0.0	18.59785667721368	0.0
31	3.975005167506718E-4	0.0	0.0	19.083801058941376	0.0
32	3.975005167506718E-4	0.0	0.0	19.569248565023134	0.0
33	3.975005167506718E-4	0.0	0.0	20.047441686674194	0.0
34	3.975005167506718E-4	0.0	0.0	20.54988233984704	0.0
35	3.975005167506718E-4	0.0	0.0	21.07706740018762	0.0
36	4.968756459383397E-4	0.0	0.0	21.568278663762264	0.0
37	4.968756459383397E-4	0.0	0.0	22.05243429316458	0.0
38	4.968756459383397E-4	0.0	0.0	22.543148681093285	0.0
39	4.968756459383397E-4	0.0	0.0	23.058408725931343	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	60	0.0	45.000004	1
AACCGGT	20	7.032867E-4	45.0	36
ATTCGCG	25	3.890458E-5	45.0	1
CACGATA	40	6.8139343E-9	45.0	34
TAATGCG	20	7.032867E-4	45.0	1
CGTTTTT	4355	0.0	42.365097	1
TCCCGAT	60	3.6379788E-12	41.250004	44
CGAATAT	230	0.0	41.08696	14
TAGCGAG	55	6.002665E-11	40.909092	1
AGCTGCG	50	1.0804797E-9	40.5	1
CATACGG	95	0.0	40.26316	2
ATTAGCG	45	1.9279469E-8	40.0	1
TATAGCG	45	1.9279469E-8	40.0	1
ACGGGCC	85	0.0	39.705883	5
TTTCGCG	40	3.4576442E-7	39.375	1
CGTTGAT	80	0.0	39.375	25
TGCGTTG	80	0.0	39.375	1
CGACGGT	35	6.2475156E-6	38.571426	28
ATATGCG	35	6.2475156E-6	38.571426	1
CTACGAA	240	0.0	38.437504	11
>>END_MODULE