FastQCFastQC Report
Sat 18 Jun 2016
SRR3551219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659523
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC22850.3464625191236697TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC18980.2877837467381729TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT14240.2159136224210528Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.19377641113350103No Hit
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC10300.1561734768916323TruSeq Adapter, Index 27 (95% over 22bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC8270.12539365571784458No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8170.12387740836938212No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC8130.12327090942999713No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC6770.10264994549090782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT351.210883E-745.00000428
TAACGCA207.0310966E-445.00000424
CGGATTA207.0310966E-445.0000046
CGCTATT207.0310966E-445.00000437
ACTACGG406.8066583E-945.0000042
GAACGTA207.0310966E-445.0000049
ATGACGG302.164088E-645.0000042
TTACGAT207.0310966E-445.00000427
CCACGCA207.0310966E-445.00000431
CCTACGG253.8889906E-545.02
ACGATAG253.8889906E-545.01
AGGTTCG253.8889906E-545.01
CGTAAGG750.042.02
TACGAGG650.041.538462
TTGCGAG650.041.538461
GCTACGA1550.040.6451610
TAGCACG451.9261279E-840.01
TTAAGCG451.9261279E-840.01
CGATACG451.9261279E-840.010
CTCGTAC403.455225E-739.37500429
Produced by FastQC (version 0.11.5)