##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	659523
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.377592593434954	33.0	31.0	34.0	30.0	34.0
2	32.484914097006474	34.0	31.0	34.0	31.0	34.0
3	32.500539025932376	34.0	31.0	34.0	31.0	34.0
4	35.992274719759585	37.0	35.0	37.0	35.0	37.0
5	36.040494417935385	37.0	35.0	37.0	35.0	37.0
6	36.08090999100865	37.0	35.0	37.0	35.0	37.0
7	36.251549983851966	37.0	37.0	37.0	35.0	37.0
8	35.997006927734134	37.0	37.0	37.0	35.0	37.0
9	37.87326901412081	39.0	38.0	39.0	35.0	39.0
10	37.68912380614474	39.0	37.0	39.0	35.0	39.0
11	37.620527259852956	39.0	37.0	39.0	35.0	39.0
12	37.5652570115068	39.0	37.0	39.0	35.0	39.0
13	37.53592823904549	39.0	37.0	39.0	35.0	39.0
14	38.82078714464848	40.0	38.0	41.0	35.0	41.0
15	38.89223423595538	40.0	38.0	41.0	35.0	41.0
16	38.953155538169256	40.0	38.0	41.0	35.0	41.0
17	38.94832174162235	40.0	38.0	41.0	35.0	41.0
18	38.94645372488905	40.0	38.0	41.0	35.0	41.0
19	38.946341522585264	40.0	38.0	41.0	35.0	41.0
20	38.90844595260514	40.0	38.0	41.0	35.0	41.0
21	38.86447326325238	40.0	38.0	41.0	35.0	41.0
22	38.85749852544945	40.0	38.0	41.0	35.0	41.0
23	38.82271581127573	40.0	38.0	41.0	35.0	41.0
24	38.77834434887032	40.0	38.0	41.0	35.0	41.0
25	38.71236029675993	40.0	38.0	41.0	35.0	41.0
26	38.709188307306945	40.0	38.0	41.0	35.0	41.0
27	38.68363044200127	40.0	38.0	41.0	35.0	41.0
28	38.644088227400715	40.0	38.0	41.0	35.0	41.0
29	38.63228879053498	40.0	38.0	41.0	35.0	41.0
30	38.567607194896915	40.0	38.0	41.0	35.0	41.0
31	38.52972981988498	40.0	38.0	41.0	35.0	41.0
32	38.470926715216905	40.0	38.0	41.0	35.0	41.0
33	38.44304141023133	40.0	38.0	41.0	35.0	41.0
34	38.38710249680451	40.0	38.0	41.0	35.0	41.0
35	38.3313925367273	40.0	38.0	41.0	34.0	41.0
36	38.30666254247388	40.0	38.0	41.0	34.0	41.0
37	38.25549980819471	40.0	38.0	41.0	34.0	41.0
38	38.17564057659854	40.0	37.0	41.0	34.0	41.0
39	38.147943286284175	40.0	37.0	41.0	34.0	41.0
40	38.079883491553744	40.0	37.0	41.0	34.0	41.0
41	38.01920782141032	40.0	37.0	41.0	34.0	41.0
42	38.00667452082793	40.0	37.0	41.0	34.0	41.0
43	37.94658412216101	40.0	37.0	41.0	34.0	41.0
44	37.884078341468	40.0	37.0	41.0	34.0	41.0
45	37.830034737226754	40.0	37.0	41.0	34.0	41.0
46	37.711593075601606	40.0	36.0	41.0	33.0	41.0
47	37.66649836321099	40.0	36.0	41.0	33.0	41.0
48	37.64712678708703	40.0	36.0	41.0	33.0	41.0
49	37.5622502930148	40.0	36.0	41.0	33.0	41.0
50	37.51568785319087	40.0	36.0	41.0	33.0	41.0
51	36.746360627301854	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	5.0
11	8.0
12	6.0
13	5.0
14	21.0
15	17.0
16	30.0
17	46.0
18	77.0
19	157.0
20	234.0
21	365.0
22	577.0
23	934.0
24	1399.0
25	2091.0
26	2914.0
27	3644.0
28	4145.0
29	4816.0
30	6035.0
31	7802.0
32	10355.0
33	14668.0
34	27735.0
35	43298.0
36	36468.0
37	56613.0
38	114841.0
39	319562.0
40	648.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.57966591005924	23.37719836912435	26.21531015597636	10.827825564840044
2	32.21100704600143	25.62730943424263	25.11466620572747	17.047017314028473
3	31.306262253173884	24.908911440541118	26.996935664108758	16.78789064217624
4	29.168656741311523	25.064326793758518	26.71991727354467	19.04709919138529
5	25.644291404545406	29.968932091830002	24.69314944285491	19.69362706076968
6	25.255070710195092	34.172272991237605	24.545618575849517	16.027037722717782
7	80.96214991744033	5.732931224536521	8.47188043479909	4.833038423224057
8	82.03125592284121	5.146446750151245	7.740745963370496	5.081551363637052
9	75.46575327926395	8.172118334008063	10.053478043980272	6.308650342747713
10	36.12686744814055	35.56555267973975	16.44749311244642	11.860086759673278
11	25.158940628302577	25.706457545832368	29.827162964748766	19.307438861116292
12	25.813959482838357	22.57055477974233	31.791158155212173	19.82432758220714
13	23.169624107119844	24.971987330237155	32.330790586529965	19.52759797611304
14	18.652723256050205	28.730612882340722	30.274910806749727	22.341753054859346
15	17.51553774470337	29.20625967555339	33.63279218465467	19.64541039508857
16	20.767130183481093	27.76809906553676	31.483056694004606	19.98171405697754
17	20.275562793109568	28.11698758041797	28.891031851808048	22.716417774664414
18	20.27738228992772	28.738194119083033	30.587257760532992	20.397165830456252
19	21.398950453585392	28.565038671888622	28.688461206053468	21.347549668472517
20	23.527761730826676	28.556850936206924	29.660982255357283	18.25440507760912
21	23.12246881458266	27.26045945327153	30.418651055383965	19.19842067676184
22	21.331932320783352	25.88795235344332	29.2462886055528	23.533826720220524
23	20.452508858675134	27.213304160734346	30.332679830726146	22.00150714986437
24	21.467181584266203	26.421974669571796	30.252925220197017	21.85791852596498
25	20.43158464526635	29.11740758093349	27.739745240120513	22.711262533679644
26	19.030420470552205	29.68509058819783	28.973212458094714	22.31127648315525
27	20.603527094581995	28.362922900338578	29.62216632323664	21.411383681842786
28	19.143684147482347	28.237832494090426	30.81803060696898	21.800452751458252
29	19.909389058455883	26.56677629134996	30.83804507196868	22.685789578225474
30	20.399440201478946	27.096856364372435	29.881292995088877	22.622410439059745
31	22.055030681265094	28.10849659526658	27.42936940789025	22.407103315578077
32	22.418323545956696	29.24568210661342	27.134004424409763	21.201989923020122
33	21.17575884389172	27.492900171790822	28.117594079357357	23.2137469049601
34	20.221432762769457	26.432436776276187	30.53145985810957	22.81467060284478
35	20.791541765791337	25.950724993669667	29.478729324072095	23.779003916466902
36	20.96136146881913	26.90596082320101	28.376265877005046	23.756411830974812
37	21.58969437002197	27.487593306071208	29.92496091872459	20.99775140518223
38	21.143311150634624	27.726857137658577	28.14412840795545	22.985703303751347
39	21.318134469912344	25.537699215948496	30.67338060992566	22.4707857042135
40	21.849882415018126	24.372008254450563	32.51137564573184	21.26673368479947
41	20.063136539589976	25.264623068490412	30.132838430198795	24.53940196172082
42	21.203809419838276	25.410182813942804	29.11073609260026	24.27527167361866
43	22.610280460272044	24.921344668798508	28.191890199432013	24.276484671497432
44	21.386668850062847	25.89735308700379	28.331233330755712	24.384744732177648
45	20.747722217420772	25.278420919361416	28.647370902910136	25.326485960307675
46	21.760272196723996	25.615027830720084	29.665682622137513	22.95901735041841
47	20.004761016674173	24.95849272883584	32.10486973160906	22.93187652288093
48	20.302855245381892	23.99264316786526	30.750709224697243	24.95379236205561
49	20.737108485981533	24.152758887862895	31.17359061018342	23.93654201597215
50	19.813107351828517	23.86709788741257	31.32142472665851	24.998370034100404
51	19.80340336879836	23.03376834469761	29.260996204832885	27.901832081671145
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	218.0
1	516.5
2	815.0
3	1603.5
4	2392.0
5	1669.5
6	947.0
7	930.0
8	913.0
9	929.0
10	945.0
11	965.5
12	986.0
13	1039.5
14	1093.0
15	1151.5
16	1210.0
17	1177.0
18	1144.0
19	1433.0
20	1722.0
21	2102.0
22	2482.0
23	2823.5
24	3165.0
25	4289.0
26	6784.5
27	8156.0
28	9341.5
29	10527.0
30	12094.0
31	13661.0
32	15736.0
33	17811.0
34	20697.5
35	23584.0
36	25370.5
37	27157.0
38	28848.5
39	30540.0
40	33084.5
41	35629.0
42	39228.0
43	42827.0
44	46684.5
45	50542.0
46	56557.5
47	62573.0
48	67169.0
49	71765.0
50	67434.0
51	63103.0
52	53216.0
53	43329.0
54	37189.5
55	31050.0
56	27746.5
57	24443.0
58	22527.5
59	20612.0
60	19960.5
61	19309.0
62	17386.5
63	15464.0
64	12759.5
65	10055.0
66	7898.5
67	5742.0
68	4698.5
69	3655.0
70	2838.0
71	2021.0
72	1601.5
73	1182.0
74	915.0
75	521.0
76	394.0
77	256.5
78	119.0
79	124.5
80	130.0
81	85.0
82	40.0
83	21.0
84	2.0
85	2.0
86	2.0
87	2.5
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	659523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.869201383128946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.55934438247287	21.075512668216305
2	11.382192861228441	6.7995402150728985
3	4.133208100355482	3.703668753236932
4	2.0718184490584552	2.475342499368355
5	1.2744435273550503	1.90333051849966
6	0.8937480963974275	1.6017325126249742
7	0.7125934271155867	1.4899217605166635
8	0.6189511310470964	1.4790060783648924
9	0.5228926602493118	1.4056547553672045
>10	7.5967838832907715	50.17836715892755
>50	0.18013649557414566	3.3739469263017763
>100	0.0482416825752886	2.644432459132574
>500	0.0030792563345928897	0.6562411081993246
>1k	0.0025660469454940747	1.2133025861709075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2285	0.3464625191236697	TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	1898	0.2877837467381729	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	1424	0.2159136224210528	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1278	0.19377641113350103	No Hit
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	1030	0.1561734768916323	TruSeq Adapter, Index 27 (95% over 22bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	827	0.12539365571784458	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	817	0.12387740836938212	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	813	0.12327090942999713	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	677	0.10264994549090782	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.548742045387348E-4	0.0	0.0	0.22349485916336503	0.0
2	4.548742045387348E-4	0.0	0.0	1.134153016649912	0.0
3	4.548742045387348E-4	0.0	0.0	1.593424338499188	0.0
4	4.548742045387348E-4	0.0	0.0	2.177179567657231	0.0
5	4.548742045387348E-4	0.0	0.0	3.8094198382770577	0.0
6	4.548742045387348E-4	0.0	0.0	4.682020187317198	0.0
7	4.548742045387348E-4	0.0	0.0	5.489725149843144	0.0
8	4.548742045387348E-4	0.0	0.0	6.662845723348541	0.0
9	4.548742045387348E-4	0.0	0.0	7.17366945504554	0.0
10	4.548742045387348E-4	0.0	0.0	8.366956118285488	0.0
11	4.548742045387348E-4	0.0	0.0	10.052568295571193	0.0
12	4.548742045387348E-4	0.0	0.0	11.393234201081691	0.0
13	4.548742045387348E-4	0.0	0.0	11.922556150429932	0.0
14	4.548742045387348E-4	0.0	0.0	12.140895768608525	0.0
15	4.548742045387348E-4	0.0	0.0	12.537849324435994	0.0
16	4.548742045387348E-4	0.0	0.0	13.310074099007918	0.0
17	4.548742045387348E-4	0.0	0.0	14.306248606947749	0.0
18	4.548742045387348E-4	0.0	0.0	15.326683072462977	0.0
19	4.548742045387348E-4	0.0	0.0	16.00853950506654	0.0
20	4.548742045387348E-4	0.0	0.0	16.63854027835269	0.0
21	4.548742045387348E-4	0.0	0.0	17.391811961068832	0.0
22	4.548742045387348E-4	0.0	0.0	18.24303322249565	0.0
23	4.548742045387348E-4	0.0	0.0	19.035120837332435	0.0
24	4.548742045387348E-4	0.0	0.0	19.622969934331326	0.0
25	4.548742045387348E-4	0.0	0.0	20.156082502050726	0.0
26	4.548742045387348E-4	0.0	0.0	20.6274838026877	0.0
27	4.548742045387348E-4	0.0	0.0	21.114199201544146	0.0
28	4.548742045387348E-4	0.0	0.0	21.62199043854422	0.0
29	4.548742045387348E-4	0.0	0.0	22.151767262097	0.0
30	4.548742045387348E-4	0.0	0.0	22.735522491255043	0.0
31	4.548742045387348E-4	0.0	0.0	23.279703664618218	0.0
32	6.064989393849798E-4	0.0	0.0	23.811906483928535	0.0
33	7.581236742312246E-4	0.0	0.0	24.2898276481639	0.0
34	7.581236742312246E-4	0.0	0.0	24.790795772095894	0.0
35	7.581236742312246E-4	0.0	0.0	25.327092459247062	0.0
36	7.581236742312246E-4	0.0	0.0	25.846558800830298	0.0
37	7.581236742312246E-4	0.0	0.0	26.352985415216754	0.0
38	7.581236742312246E-4	0.0	0.0	26.838639440929278	0.0
39	7.581236742312246E-4	0.0	0.0	27.34006243906581	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	35	1.210883E-7	45.000004	28
TAACGCA	20	7.0310966E-4	45.000004	24
CGGATTA	20	7.0310966E-4	45.000004	6
CGCTATT	20	7.0310966E-4	45.000004	37
ACTACGG	40	6.8066583E-9	45.000004	2
GAACGTA	20	7.0310966E-4	45.000004	9
ATGACGG	30	2.164088E-6	45.000004	2
TTACGAT	20	7.0310966E-4	45.000004	27
CCACGCA	20	7.0310966E-4	45.000004	31
CCTACGG	25	3.8889906E-5	45.0	2
ACGATAG	25	3.8889906E-5	45.0	1
AGGTTCG	25	3.8889906E-5	45.0	1
CGTAAGG	75	0.0	42.0	2
TACGAGG	65	0.0	41.53846	2
TTGCGAG	65	0.0	41.53846	1
GCTACGA	155	0.0	40.64516	10
TAGCACG	45	1.9261279E-8	40.0	1
TTAAGCG	45	1.9261279E-8	40.0	1
CGATACG	45	1.9261279E-8	40.0	10
CTCGTAC	40	3.455225E-7	39.375004	29
>>END_MODULE