FastQCFastQC Report
Sat 18 Jun 2016
SRR3551217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148923
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90180.7849089973827663No Hit
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC18330.15954071769822695RNA PCR Primer, Index 40 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCC12050.10488083187472094No Hit
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT11730.10209561476269514RNA PCR Primer, Index 46 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG302.1655014E-645.0000041
TATGCGA207.0332875E-445.024
TACGACT207.0332875E-445.044
TACGACG253.8908052E-545.01
TCGCTAG351.2119199E-745.01
CGTTTTT49150.042.6195341
TTGAGCG650.041.5384641
CTCGCGG556.184564E-1140.9090922
CGTATGG850.039.7058832
CGTAAGG800.039.3752
TGTTCGG356.2482886E-638.5714262
ACGTCGG356.2482886E-638.5714262
ATCGGTT356.2482886E-638.57142611
ACGATAG601.5643309E-1037.5000041
GTATGCG1200.037.5000041
GCGTAAG552.748493E-936.818181
ACGACAG552.748493E-936.818181
TACGAAT1350.036.66666412
TAGGGCG2400.036.5625045
GTAAGCG800.036.56251
Produced by FastQC (version 0.11.5)