FastQCFastQC Report
Sat 18 Jun 2016
SRR3551212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725078
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG71600.9874799676724435No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC64150.8847324012037326No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC59850.8254284366647451No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC27520.3795453730495202No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.24480124896907643No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGGAT16870.23266462366807436No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT15430.21280469135734362No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC14570.20094389844954613No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT12050.16618901690576737Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT11280.15556946976739056No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTA10450.14412242544939993No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTC9850.13584745365326215No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8210.11322919741048548No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7940.10950546010222348No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC7390.10192006928909716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG1000.045.00000440
GTTACGT253.8893748E-545.00000445
CGAGTAA207.031562E-445.024
TAACGGT207.031562E-445.028
GTAGACG406.8084773E-945.01
ACTACGC351.2111013E-745.014
CGACTTA207.031562E-445.011
CGATCGA950.045.041
TTCGAAA207.031562E-445.022
ACGACGT207.031562E-445.024
CGGTTAT302.16439E-644.99999638
CCGATGA6900.043.36956818
CGATGAA7500.042.319
TATAGCG750.042.01
GCGATAT603.6379788E-1241.2499969
GATGAAT8300.041.2048220
TACGGGT501.0804797E-940.5000044
TACGGCT6850.040.4014637
GCTACGA1400.040.1785710
TTTCGCG403.4558616E-739.3751