##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	725078
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.369847106104444	33.0	31.0	34.0	30.0	34.0
2	32.466891837843654	34.0	31.0	34.0	30.0	34.0
3	32.49820019363434	34.0	31.0	34.0	30.0	34.0
4	35.98885499215257	37.0	35.0	37.0	35.0	37.0
5	36.01482185364885	37.0	35.0	37.0	35.0	37.0
6	36.03481418550832	37.0	35.0	37.0	35.0	37.0
7	36.165829055632635	37.0	36.0	37.0	35.0	37.0
8	35.725097161960505	37.0	35.0	37.0	35.0	37.0
9	37.67485429153829	39.0	38.0	39.0	35.0	39.0
10	37.602082810401086	39.0	37.0	39.0	35.0	39.0
11	37.528118354163276	39.0	37.0	39.0	35.0	39.0
12	37.37389632563669	39.0	37.0	39.0	35.0	39.0
13	37.28344122977114	39.0	37.0	39.0	34.0	39.0
14	38.56891810260414	40.0	38.0	41.0	35.0	41.0
15	38.59473049796022	40.0	38.0	41.0	35.0	41.0
16	38.71570644813386	40.0	38.0	41.0	35.0	41.0
17	38.66521394939579	40.0	38.0	41.0	35.0	41.0
18	38.66987137935505	40.0	38.0	41.0	35.0	41.0
19	38.65493505526302	40.0	38.0	41.0	35.0	41.0
20	38.487393080468586	40.0	38.0	41.0	34.0	41.0
21	38.53229721492033	40.0	38.0	41.0	35.0	41.0
22	38.451128016572014	40.0	37.0	41.0	34.0	41.0
23	38.37747387177655	40.0	37.0	41.0	34.0	41.0
24	38.35551347579157	40.0	37.0	41.0	34.0	41.0
25	38.36028123870811	40.0	37.0	41.0	34.0	41.0
26	38.296235715329935	40.0	37.0	41.0	34.0	41.0
27	38.19366468159288	40.0	37.0	41.0	34.0	41.0
28	38.118714124549356	40.0	37.0	41.0	34.0	41.0
29	38.12937366738475	40.0	37.0	41.0	34.0	41.0
30	38.05363422969667	40.0	37.0	41.0	34.0	41.0
31	38.01669889308461	40.0	37.0	41.0	34.0	41.0
32	37.85999851050508	40.0	36.0	41.0	34.0	41.0
33	37.77342161808799	40.0	36.0	41.0	34.0	41.0
34	37.662159105641045	40.0	36.0	41.0	33.0	41.0
35	37.5981287530445	40.0	36.0	41.0	33.0	41.0
36	37.52939683730578	40.0	36.0	41.0	33.0	41.0
37	37.439930600569866	40.0	36.0	41.0	33.0	41.0
38	37.384964100414024	40.0	35.0	41.0	33.0	41.0
39	37.301388264435	40.0	35.0	41.0	33.0	41.0
40	37.231748860122636	40.0	35.0	41.0	33.0	41.0
41	37.14930393695575	40.0	35.0	41.0	32.0	41.0
42	37.11161833623417	40.0	35.0	41.0	32.0	41.0
43	37.06170922300773	40.0	35.0	41.0	32.0	41.0
44	37.063718661992226	40.0	35.0	41.0	32.0	41.0
45	37.00544768976579	39.0	35.0	41.0	32.0	41.0
46	36.87412526652305	39.0	35.0	41.0	32.0	41.0
47	36.751590863327806	39.0	35.0	41.0	31.0	41.0
48	36.732209500219284	39.0	35.0	41.0	32.0	41.0
49	36.63458276213042	39.0	35.0	41.0	31.0	41.0
50	36.560604514272946	39.0	35.0	41.0	31.0	41.0
51	35.77866795020674	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	9.0
10	8.0
11	19.0
12	12.0
13	17.0
14	30.0
15	35.0
16	37.0
17	86.0
18	140.0
19	252.0
20	579.0
21	1142.0
22	2183.0
23	2968.0
24	3694.0
25	4532.0
26	5069.0
27	5707.0
28	5751.0
29	6460.0
30	7826.0
31	9921.0
32	13394.0
33	18831.0
34	37284.0
35	56928.0
36	43275.0
37	65494.0
38	120996.0
39	311658.0
40	735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.2166828948058	19.130769379294364	22.64901155461895	12.003536171280883
2	30.578503278267995	28.211171763589572	24.132300249076653	17.078024709065783
3	30.439483752092876	28.123043313960704	24.964210746981706	16.473262186964714
4	27.75397957185296	24.18815630870058	29.57888668529455	18.47897743415191
5	29.10335163940983	28.148695726528732	23.217364200817016	19.53058843324442
6	25.01013684045027	36.64460926962341	23.73041245217756	14.614841437748765
7	76.2011811143077	6.0936892306758725	13.04107971831996	4.664049936696466
8	75.7692275865493	9.865559291552081	9.036545033775676	5.3286680881229325
9	70.14969423979214	8.032515122510958	10.529763694388741	11.288026943308168
10	42.25724680655047	24.979795277197763	18.294859311687848	14.468098604563922
11	33.341792193391605	24.69858415232568	23.046761865619974	18.912861788662738
12	30.55326461428977	21.535752015645212	26.48390931734241	21.427074052722602
13	26.542937449488193	23.51140153197311	29.391044825522222	20.554616193016475
14	19.820764110895656	30.41962381978215	26.650098334248177	23.10951373507402
15	18.895070599301043	26.31909946240267	34.119363709835355	20.66646622846094
16	21.097592259039715	25.14226055679527	30.131930633669757	23.628216550495257
17	21.247645080943016	26.871591745991463	28.61374914147168	23.267014031593842
18	21.860544658643622	26.845387668637027	27.681159820046947	23.6129078526724
19	24.38068731915739	27.977403810348676	25.46346737868202	22.178441491811913
20	27.994229586334157	25.864803510794697	27.95823345902096	18.18273344385018
21	25.393681783201256	28.05560229382218	26.373714276257175	20.177001646719386
22	21.85185593825768	25.240732721169305	28.213102590342004	24.69430875023101
23	24.430337149934214	27.431255671803584	26.861248031246294	21.277159147015904
24	23.29514893570071	25.519185522109346	27.272789961907545	23.912875580282396
25	22.19981850228527	29.4077326853111	25.591867357718755	22.800581454684878
26	21.338118105914123	27.987195860307445	28.391152400155566	22.283533633622866
27	25.091093647855818	27.34326513837132	26.564452376158147	21.001188837614713
28	19.967369027883898	28.641884045578546	29.974016588560126	21.416730337977434
29	22.835474252425257	25.106540261875278	30.3324607835295	21.725524702169974
30	23.530847715694037	27.033643276999165	27.674539842610034	21.760969164696764
31	23.51774567701682	26.273725033720506	26.305997423725447	23.902531865537224
32	26.133740094169177	27.163836166591732	26.545833689616842	20.156590049622245
33	24.61638609915071	26.569417359235835	24.856222365042104	23.957974176571348
34	23.79013016530635	25.495739768686953	28.513484066541807	22.200645999464886
35	25.5164271981773	24.17615759959618	26.748708414818818	23.558706787407697
36	22.527921134002135	28.981847470203203	27.173214467960687	21.317016927833972
37	25.01330891297212	26.65823538984771	28.79676393436292	19.531691762817243
38	22.383384959962928	25.872802650197634	26.517698785509975	25.226113604329463
39	24.352276582657314	25.612554787209103	27.652886999743476	22.38228163039011
40	25.11150524495296	23.423686830934052	28.750837840894363	22.713970083218634
41	22.488339185577274	27.11225550906247	27.14769997158926	23.251705333770985
42	23.92377095981398	25.617381854090183	28.60023335420465	21.85861383189119
43	23.625044477973404	26.780704972430552	26.723331834644	22.870918714952047
44	23.019868207282528	25.495601852490353	28.05353355087315	23.43099638935397
45	22.87767660858556	23.91563390421444	27.134460016715444	26.072229470484555
46	23.92335721122417	27.17762778625196	26.515767958757543	22.383247043766325
47	21.929089008354964	25.778054223131857	30.641117231525435	21.651739536987744
48	23.201090089617946	24.000728197518058	28.381222434000204	24.416959278863793
49	21.819583548252737	24.410063469033677	30.61422357318799	23.156129409525597
50	21.185720708668583	24.68906793476012	29.155208129332294	24.970003227239
51	21.75048753375499	23.859915760787114	27.06812232614974	27.321474379308157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	323.0
1	752.0
2	1181.0
3	2348.5
4	3516.0
5	2609.0
6	1702.0
7	1581.0
8	1460.0
9	1461.0
10	1462.0
11	1427.0
12	1392.0
13	1325.0
14	1258.0
15	1297.5
16	1337.0
17	1486.5
18	1636.0
19	1649.5
20	1663.0
21	1812.0
22	1961.0
23	2413.0
24	2865.0
25	3566.0
26	5655.5
27	7044.0
28	7950.5
29	8857.0
30	10488.0
31	12119.0
32	13352.0
33	14585.0
34	15935.0
35	17285.0
36	19163.0
37	21041.0
38	23094.5
39	25148.0
40	28298.5
41	31449.0
42	34270.5
43	37092.0
44	42319.0
45	47546.0
46	62736.0
47	77926.0
48	71738.5
49	65551.0
50	64112.5
51	62674.0
52	55318.5
53	47963.0
54	44800.0
55	41637.0
56	39162.5
57	36688.0
58	34947.5
59	33207.0
60	33161.0
61	33115.0
62	30477.5
63	27840.0
64	23067.0
65	18294.0
66	15141.5
67	11989.0
68	9497.0
69	7005.0
70	6188.5
71	5372.0
72	4294.0
73	3216.0
74	2561.5
75	1577.5
76	1248.0
77	1018.5
78	789.0
79	490.5
80	192.0
81	198.0
82	204.0
83	115.5
84	27.0
85	17.5
86	8.0
87	15.0
88	22.0
89	11.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	3.0
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	725078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.320753185706064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.59800799860908	18.38236242306695
2	10.316673407128118	5.224518820788567
3	3.663517525671836	2.782890691771354
4	1.892565400275199	1.9168472555270124
5	1.099403576345076	1.3918863304058111
6	0.7847640963095456	1.1922490794994598
7	0.5828228718613768	1.0330259862570417
8	0.4816028885626427	0.9755638299854221
9	0.4004059638729707	0.9124722526781007
>10	7.4020520994772525	47.159347940679964
>50	0.6989908710023269	10.59139546928542
>100	0.06764427783893273	2.99476131637868
>500	0.005499534783653068	0.9654366319377563
>1k	0.004399627826922455	1.753465753549723
>5k	0.0016498604350959204	2.7237762181887373
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG	7160	0.9874799676724435	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC	6415	0.8847324012037326	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC	5985	0.8254284366647451	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	2752	0.3795453730495202	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1775	0.24480124896907643	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGGAT	1687	0.23266462366807436	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT	1543	0.21280469135734362	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	1457	0.20094389844954613	No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	1205	0.16618901690576737	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	1128	0.15556946976739056	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTA	1045	0.14412242544939993	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTC	985	0.13584745365326215	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	821	0.11322919741048548	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	794	0.10950546010222348	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	739	0.10192006928909716	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.379161966022966E-4	0.0	0.0	0.16991275421402938	0.0
2	1.379161966022966E-4	0.0	0.0	0.8946623673590979	0.0
3	1.379161966022966E-4	0.0	0.0	1.151876074022381	0.0
4	1.379161966022966E-4	0.0	0.0	1.6067236904167552	0.0
5	1.379161966022966E-4	0.0	0.0	3.3869459561591992	0.0
6	1.379161966022966E-4	0.0	0.0	3.9471615467577283	0.0
7	1.379161966022966E-4	0.0	0.0	4.68832318729847	0.0
8	1.379161966022966E-4	0.0	0.0	5.585605962393012	0.0
9	1.379161966022966E-4	0.0	0.0	5.929706872915742	0.0
10	1.379161966022966E-4	0.0	0.0	7.671312603609542	0.0
11	1.379161966022966E-4	0.0	0.0	8.748576015270082	0.0
12	1.379161966022966E-4	0.0	0.0	10.425636965954007	0.0
13	1.379161966022966E-4	0.0	0.0	10.812491897423449	0.0
14	1.379161966022966E-4	0.0	0.0	10.985025059372923	0.0
15	1.379161966022966E-4	0.0	0.0	11.41118610687402	0.0
16	1.379161966022966E-4	0.0	0.0	11.966022965805058	0.0
17	1.379161966022966E-4	0.0	0.0	12.583611694190143	0.0
18	1.379161966022966E-4	0.0	0.0	13.225059924587423	0.0
19	1.379161966022966E-4	0.0	0.0	13.886230171098834	0.0
20	1.379161966022966E-4	0.0	0.0	14.32921699458541	0.0
21	1.379161966022966E-4	0.0	0.0	14.825163637567268	0.0
22	1.379161966022966E-4	0.0	0.0	15.38317256902016	0.0
23	1.379161966022966E-4	0.0	0.0	15.883808362686498	0.0
24	1.379161966022966E-4	0.0	0.0	16.2985223658696	0.0
25	1.379161966022966E-4	0.0	0.0	16.670482348105995	0.0
26	1.379161966022966E-4	0.0	0.0	17.023271979014673	0.0
27	1.379161966022966E-4	0.0	0.0	17.40998899428751	0.0
28	2.758323932045932E-4	0.0	0.0	17.749262837929162	0.0
29	2.758323932045932E-4	0.0	0.0	18.116809501874283	0.0
30	2.758323932045932E-4	0.0	0.0	18.52876518112534	0.0
31	4.1374858980688977E-4	0.0	0.0	18.909827632337485	0.0
32	4.1374858980688977E-4	0.0	0.0	19.301785463081213	0.0
33	4.1374858980688977E-4	0.0	0.0	19.675814188266642	0.0
34	4.1374858980688977E-4	0.0	0.0	20.06666868943755	0.0
35	4.1374858980688977E-4	0.0	0.0	20.46069526313031	0.0
36	4.1374858980688977E-4	0.0	0.0	20.863824305798826	0.0
37	4.1374858980688977E-4	0.0	0.0	21.246403835173595	0.0
38	5.516647864091864E-4	0.0	0.0	21.618501733606593	0.0
39	5.516647864091864E-4	0.0	0.0	22.030871161447457	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	100	0.0	45.000004	40
GTTACGT	25	3.8893748E-5	45.000004	45
CGAGTAA	20	7.031562E-4	45.0	24
TAACGGT	20	7.031562E-4	45.0	28
GTAGACG	40	6.8084773E-9	45.0	1
ACTACGC	35	1.2111013E-7	45.0	14
CGACTTA	20	7.031562E-4	45.0	11
CGATCGA	95	0.0	45.0	41
TTCGAAA	20	7.031562E-4	45.0	22
ACGACGT	20	7.031562E-4	45.0	24
CGGTTAT	30	2.16439E-6	44.999996	38
CCGATGA	690	0.0	43.369568	18
CGATGAA	750	0.0	42.3	19
TATAGCG	75	0.0	42.0	1
GCGATAT	60	3.6379788E-12	41.249996	9
GATGAAT	830	0.0	41.20482	20
TACGGGT	50	1.0804797E-9	40.500004	4
TACGGCT	685	0.0	40.401463	7
GCTACGA	140	0.0	40.17857	10
TTTCGCG	40	3.4558616E-7	39.375	1
>>END_MODULE