##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	637219
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46392370597864	33.0	31.0	34.0	31.0	34.0
2	32.576377979940965	34.0	31.0	34.0	31.0	34.0
3	32.59479550986396	34.0	31.0	34.0	31.0	34.0
4	36.05916647180953	37.0	35.0	37.0	35.0	37.0
5	36.084354044684794	37.0	35.0	37.0	35.0	37.0
6	36.12439051566259	37.0	36.0	37.0	35.0	37.0
7	36.24282232639014	37.0	37.0	37.0	35.0	37.0
8	35.92767949480477	37.0	37.0	37.0	35.0	37.0
9	37.81809079766925	39.0	38.0	39.0	35.0	39.0
10	37.69268650181492	39.0	37.0	39.0	35.0	39.0
11	37.622881615268845	39.0	37.0	39.0	35.0	39.0
12	37.51034573670904	39.0	37.0	39.0	35.0	39.0
13	37.47096681046862	39.0	37.0	39.0	35.0	39.0
14	38.769713395237744	40.0	38.0	41.0	35.0	41.0
15	38.836753141384676	40.0	38.0	41.0	35.0	41.0
16	38.89935328356499	40.0	38.0	41.0	35.0	41.0
17	38.87736712182154	40.0	38.0	41.0	35.0	41.0
18	38.881031482112114	40.0	38.0	41.0	35.0	41.0
19	38.87015610017906	40.0	38.0	41.0	35.0	41.0
20	38.7842688306532	40.0	38.0	41.0	35.0	41.0
21	38.744149185758744	40.0	38.0	41.0	35.0	41.0
22	38.71953441438501	40.0	38.0	41.0	35.0	41.0
23	38.678360187000074	40.0	38.0	41.0	35.0	41.0
24	38.64897625463146	40.0	38.0	41.0	35.0	41.0
25	38.59639150747231	40.0	38.0	41.0	35.0	41.0
26	38.54933704111146	40.0	38.0	41.0	35.0	41.0
27	38.49401226266009	40.0	38.0	41.0	35.0	41.0
28	38.43339887856451	40.0	38.0	41.0	35.0	41.0
29	38.41738397630956	40.0	38.0	41.0	35.0	41.0
30	38.36525747035164	40.0	37.0	41.0	34.0	41.0
31	38.33096156894255	40.0	37.0	41.0	34.0	41.0
32	38.25343720133894	40.0	37.0	41.0	34.0	41.0
33	38.207248999166694	40.0	37.0	41.0	34.0	41.0
34	38.139421454790266	40.0	37.0	41.0	34.0	41.0
35	38.06883975524898	40.0	37.0	41.0	34.0	41.0
36	38.0248391840168	40.0	37.0	41.0	34.0	41.0
37	37.958810079423245	40.0	37.0	41.0	34.0	41.0
38	37.8827043763604	40.0	37.0	41.0	34.0	41.0
39	37.834490183123854	40.0	36.0	41.0	34.0	41.0
40	37.73733677118855	40.0	36.0	41.0	33.0	41.0
41	37.69989595413821	40.0	36.0	41.0	33.0	41.0
42	37.66058607794181	40.0	36.0	41.0	33.0	41.0
43	37.57680326543935	40.0	36.0	41.0	33.0	41.0
44	37.537758604184745	40.0	36.0	41.0	33.0	41.0
45	37.485778044910774	40.0	35.0	41.0	33.0	41.0
46	37.38207900266627	40.0	35.0	41.0	33.0	41.0
47	37.34126414937408	40.0	35.0	41.0	33.0	41.0
48	37.28854757940363	40.0	35.0	41.0	33.0	41.0
49	37.18346596695955	39.0	35.0	41.0	33.0	41.0
50	37.119046983847	39.0	35.0	41.0	33.0	41.0
51	36.37539370294985	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	13.0
11	8.0
12	10.0
13	10.0
14	21.0
15	20.0
16	40.0
17	62.0
18	116.0
19	176.0
20	384.0
21	674.0
22	1018.0
23	1453.0
24	1957.0
25	2489.0
26	3249.0
27	3939.0
28	4321.0
29	4857.0
30	6060.0
31	7689.0
32	10128.0
33	14290.0
34	29391.0
35	48406.0
36	35854.0
37	55667.0
38	108549.0
39	295695.0
40	665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.258754054728435	21.394214547902685	24.25178784687839	11.09524355049049
2	32.0094033605401	25.25379814475086	25.27168838342862	17.465110111280424
3	32.31965776287274	24.703437907532575	26.054935587294164	16.92196874230053
4	28.818506667252546	25.606424165004498	26.663674498092494	18.911394669650466
5	26.095580954114677	31.069224238448633	23.68651907742864	19.14867573000805
6	26.482575064459784	36.01697375627532	22.703654473579725	14.796796705685173
7	81.35068163378682	6.3077215211724695	8.226057289566068	4.115539555474649
8	82.84530122297043	6.592082156997829	6.673843686393532	3.8887729336381995
9	76.354910948983	8.86304394564506	8.50382678482594	6.278218320545998
10	40.05624439949217	31.558224095640586	15.809164510160556	12.576366994706687
11	29.47510981310978	26.43015980377233	25.466911689701654	18.627818693416234
12	29.184628832473607	23.101476886282423	27.727202107909527	19.98669217333444
13	26.266322881144472	24.860212893840263	28.43637744637244	20.437086778642822
14	19.87307346453888	29.420968301321835	28.07559096637106	22.630367267768225
15	19.946988398023286	27.99461409656649	31.940981044193595	20.11741646121663
16	23.04655071490335	26.621302880171495	30.309830686153425	20.022315718771726
17	22.574499504879796	27.344759023192967	27.902808924404326	22.177932547522907
18	23.18119830074119	27.603696688265728	27.97515453870647	21.23995047228661
19	25.112402486429314	28.262183017141673	26.778077866479183	19.84733662994983
20	27.53982539754778	26.68721428582638	27.8814661835256	17.891494133100235
21	25.822519416401583	27.17182004930801	27.493216617834687	19.512443916455723
22	24.31173583964069	25.128880337843036	28.355871372322543	22.20351245019373
23	23.469953030276873	27.26252669804259	28.073080055679444	21.19444021600109
24	22.95286235972248	25.854376595801444	29.34061915919017	21.852141885285906
25	22.789025437094626	28.250413123274733	27.136510367707178	21.824051071923467
26	21.044570234095342	29.992985143255304	27.11344137572797	21.849003246921388
27	22.67634831980842	29.121699133265018	28.15845101919434	20.043501527732225
28	20.42107972298378	29.350662801956627	29.293696515640622	20.934560959418974
29	22.047522123477172	27.711351984168708	28.82478394398158	21.416341948372537
30	22.448640106462612	27.69848356687418	27.939060197514515	21.913816129148692
31	22.61781271431015	29.120443677919212	26.353106231923405	21.90863737584724
32	24.509783920441794	28.26798949811603	26.52934077609111	20.692885805351064
33	23.75368593842933	28.996938258275414	26.029669548459793	21.219706254835465
34	22.058507357752983	27.693618677409184	28.04106594436136	22.206808020476476
35	23.12925383580841	25.8474715913995	28.571495827964956	22.45177874482713
36	22.350557657571414	29.688066426142345	26.839281314587293	21.122094601698947
37	23.202541041619913	29.058141706383523	27.260643515023876	20.478673736972688
38	23.281163932651097	29.1016118477321	25.315629320531873	22.301594899084932
39	24.042126804128568	27.087547609220692	26.552880563824992	22.317445022825748
40	23.747565593618518	26.230699335707193	28.45866177876052	21.56307329191377
41	21.099810269310865	26.572340121685013	28.361363989460454	23.966485619543672
42	22.6181265781466	27.253581578703713	27.374889951492342	22.753401891657344
43	23.15546146615214	27.043920535953887	27.33424458467183	22.466373413222144
44	22.080163962468163	26.84508779556165	27.627236475999617	23.447511765970567
45	21.792978552114736	27.076876238781328	26.874120200433442	24.25602500867049
46	22.324820822982367	28.609787216012077	26.571241598257426	22.494150362748126
47	22.034025978509746	26.690039060354447	29.269999795988504	22.005935165147303
48	22.121593988879802	26.23556422517219	28.721993537543604	22.920848248404393
49	22.027120974107802	25.762100627884603	29.271255251334317	22.93952314667328
50	21.005023390702412	26.788121509245645	28.21777128428374	23.989083815768204
51	20.8444820383573	26.005031237298322	26.801146858458395	26.349339865885984
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	216.0
1	551.0
2	886.0
3	1812.0
4	2738.0
5	1898.5
6	1059.0
7	1056.5
8	1054.0
9	1037.0
10	1020.0
11	1022.0
12	1024.0
13	1063.5
14	1103.0
15	1051.0
16	999.0
17	1116.0
18	1233.0
19	1392.5
20	1552.0
21	1810.0
22	2068.0
23	2631.5
24	3195.0
25	3801.0
26	5411.0
27	6415.0
28	8014.5
29	9614.0
30	11144.5
31	12675.0
32	14771.5
33	16868.0
34	19109.5
35	21351.0
36	22623.0
37	23895.0
38	25673.0
39	27451.0
40	30386.0
41	33321.0
42	36183.5
43	39046.0
44	41627.0
45	44208.0
46	49684.0
47	55160.0
48	54040.0
49	52920.0
50	51424.0
51	49928.0
52	45456.0
53	40984.0
54	38214.5
55	35445.0
56	32184.5
57	28924.0
58	28614.0
59	28304.0
60	27499.5
61	26695.0
62	24281.5
63	21868.0
64	18538.5
65	15209.0
66	12251.5
67	9294.0
68	7520.5
69	5747.0
70	4675.5
71	3604.0
72	2880.0
73	2156.0
74	1961.0
75	1385.0
76	1004.0
77	713.5
78	423.0
79	345.5
80	268.0
81	166.0
82	64.0
83	46.5
84	29.0
85	22.5
86	16.0
87	11.0
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	637219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.920096273816178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28249034348279	18.735691086142626
2	9.104442811181917	4.719760683705779
3	3.336053680866402	2.5941249774802793
4	1.743498863055434	1.807666335347436
5	1.0958241917512133	1.4201934274684125
6	0.8891818998490113	1.382860826941269
7	0.6861583465441856	1.2449703281055473
8	0.6085639728698533	1.2619229412450126
9	0.5681523314283717	1.3253906815934862
>10	9.298585915698157	57.03249600984739
>50	0.3392791050206723	5.216484853380976
>100	0.04225678383831479	2.010907130504236
>500	0.0030620857853851297	0.5063777823104288
>1k	0.002449668628308104	0.7411529359270822
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1429	0.22425571114483403	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG	1109	0.1740374973125409	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC	1102	0.1729389738849595	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGC	1029	0.16148294385446763	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC	909	0.1426511136673577	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	645	0.10122108725571587	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5693191822591606E-4	0.0	0.0	0.0899219891434499	0.0
2	1.5693191822591606E-4	0.0	0.0	0.4251285664740066	0.0
3	1.5693191822591606E-4	0.0	0.0	0.581432757027019	0.0
4	1.5693191822591606E-4	0.0	0.0	0.8237356387678334	0.0
5	1.5693191822591606E-4	0.0	0.0	1.5657097481399644	0.0
6	1.5693191822591606E-4	0.0	0.0	1.9828347867844494	0.0
7	1.5693191822591606E-4	0.0	0.0	2.366533326846814	0.0
8	1.5693191822591606E-4	0.0	0.0	2.9901807698766043	0.0
9	1.5693191822591606E-4	0.0	0.0	3.2580635542882432	0.0
10	1.5693191822591606E-4	0.0	0.0	4.048529626392182	0.0
11	1.5693191822591606E-4	0.0	0.0	4.931428598331186	0.0
12	1.5693191822591606E-4	0.0	0.0	5.835356447312463	0.0
13	1.5693191822591606E-4	0.0	0.0	6.139490504834288	0.0
14	1.5693191822591606E-4	0.0	0.0	6.27084252038938	0.0
15	1.5693191822591606E-4	0.0	0.0	6.521933589550845	0.0
16	1.5693191822591606E-4	0.0	0.0	7.012031970170381	0.0
17	1.5693191822591606E-4	0.0	0.0	7.5898552930782035	0.0
18	1.5693191822591606E-4	0.0	0.0	8.203772957177987	0.0
19	1.5693191822591606E-4	0.0	0.0	8.641926872864746	0.0
20	1.5693191822591606E-4	0.0	0.0	9.032373485410824	0.0
21	1.5693191822591606E-4	0.0	0.0	9.482297294964525	0.0
22	1.5693191822591606E-4	0.0	0.0	10.002683535801664	0.0
23	1.5693191822591606E-4	0.0	0.0	10.433304719413577	0.0
24	1.5693191822591606E-4	0.0	0.0	10.826105310733045	0.0
25	1.5693191822591606E-4	0.0	0.0	11.161625751900052	0.0
26	1.5693191822591606E-4	0.0	0.0	11.474077201087852	0.0
27	1.5693191822591606E-4	0.0	0.0	11.795159905778076	0.0
28	1.5693191822591606E-4	0.0	0.0	12.13240659804557	0.0
29	1.5693191822591606E-4	0.0	0.0	12.474361247859841	0.0
30	1.5693191822591606E-4	0.0	0.0	12.872183660562538	0.0
31	1.5693191822591606E-4	0.0	0.0	13.235481051255533	0.0
32	1.5693191822591606E-4	0.0	0.0	13.574297062705286	0.0
33	1.5693191822591606E-4	0.0	0.0	13.910602163463425	0.0
34	1.5693191822591606E-4	0.0	0.0	14.247534991894467	0.0
35	1.5693191822591606E-4	0.0	0.0	14.606438288877136	0.0
36	1.5693191822591606E-4	0.0	0.0	14.984644211801594	0.0
37	1.5693191822591606E-4	0.0	0.0	15.341664325765553	0.0
38	1.5693191822591606E-4	0.0	0.0	15.70025375891177	0.0
39	1.5693191822591606E-4	0.0	0.0	16.06025557932202	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTAG	30	2.1639735E-6	45.000004	1
GGTCCGT	20	7.0309173E-4	45.0	8
CACGACA	20	7.0309173E-4	45.0	21
TATTCCG	25	3.8888422E-5	45.0	1
CGGTCGA	50	2.1827873E-11	45.0	41
CGTCACG	20	7.0309173E-4	45.0	37
ATACCGG	20	7.0309173E-4	45.0	2
ACGACAC	20	7.0309173E-4	45.0	22
GTGCCGG	20	7.0309173E-4	45.0	2
CAACGAG	55	6.002665E-11	40.909092	14
TCGTCCC	110	0.0	40.909092	38
CGTTAGG	105	0.0	40.714287	2
TAGTACG	50	1.0804797E-9	40.5	1
TACGGGA	320	0.0	40.078125	4
TGATCGG	40	3.4549885E-7	39.375	2
GAATGCG	40	3.4549885E-7	39.375	1
ATCCGGC	40	3.4549885E-7	39.375	6
AACGGGC	80	0.0	39.375	4
CTCGTCC	115	0.0	39.130432	37
CGGGTAC	35	6.2439085E-6	38.571426	6
>>END_MODULE