FastQCFastQC Report
Sat 18 Jun 2016
SRR3551210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655743
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG68901.0507165154641376No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC66321.011371833172447No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC65080.9924619858694641No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC26070.3975642896683609No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT15410.23500060237013587No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAACTAGT14930.22768066147865856No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC14810.22585067625578922No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.22173320950433323No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC10290.15692123286104465No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA10270.15661623532389976No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9800.1494487932009949No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT9730.1483813018209878No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9570.1459413215238287No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC9330.14228135107809003No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT9200.14029886708664827No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA8650.13191143481516387No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6700.10217417494353734No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG207.0310675E-445.01
CTCGTAG207.0310675E-445.01
CGCATCG453.8380676E-1045.021
TACGCGG351.2108649E-745.02
TACGCAT207.0310675E-445.027
CAAGCGT351.2108649E-745.017
GACGAAT351.2108649E-745.020
CGTCCAC207.0310675E-445.011
ATTTCGA207.0310675E-445.011
CCCGATC351.2108649E-745.041
CTCGTGA253.8889677E-544.99999612
ACGGGTA253.8889677E-544.9999965
TATAGCG253.8889677E-544.9999961
CGACTTG253.8889677E-544.99999641
CGTAAGG650.041.5384642
TACGGCT7550.041.423847
TAACGCC501.0804797E-940.49999612
CCGCATC501.0804797E-940.49999620
TGCAACG501.0804797E-940.4999961
GATTGCG451.9261279E-840.01