##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	655743
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26584042833854	33.0	31.0	34.0	30.0	34.0
2	32.37705473028306	34.0	31.0	34.0	30.0	34.0
3	32.403552916310204	34.0	31.0	34.0	30.0	34.0
4	35.926971389706026	37.0	35.0	37.0	35.0	37.0
5	35.95378677317181	37.0	35.0	37.0	35.0	37.0
6	35.966061399054205	37.0	35.0	37.0	35.0	37.0
7	36.10271402058429	37.0	35.0	37.0	35.0	37.0
8	35.59794614658487	37.0	35.0	37.0	33.0	37.0
9	37.55276228644454	39.0	37.0	39.0	35.0	39.0
10	37.504626050144644	39.0	37.0	39.0	35.0	39.0
11	37.453040291699644	39.0	37.0	39.0	35.0	39.0
12	37.289012616223125	39.0	37.0	39.0	35.0	39.0
13	37.16618248307645	39.0	37.0	39.0	34.0	39.0
14	38.412100472288685	40.0	38.0	41.0	34.0	41.0
15	38.44104778853911	40.0	38.0	41.0	34.0	41.0
16	38.584106883336915	40.0	38.0	41.0	35.0	41.0
17	38.52761218953157	40.0	38.0	41.0	34.0	41.0
18	38.541424003001175	40.0	38.0	41.0	35.0	41.0
19	38.53799735567135	40.0	38.0	41.0	34.0	41.0
20	38.36187957782241	40.0	37.0	41.0	34.0	41.0
21	38.39218108313776	40.0	37.0	41.0	34.0	41.0
22	38.341455417747504	40.0	37.0	41.0	34.0	41.0
23	38.27917797063789	40.0	37.0	41.0	34.0	41.0
24	38.22844773028458	40.0	37.0	41.0	34.0	41.0
25	38.21854446025348	40.0	37.0	41.0	34.0	41.0
26	38.17234800828983	40.0	37.0	41.0	34.0	41.0
27	38.066137190942186	40.0	37.0	41.0	34.0	41.0
28	37.98127772618236	40.0	36.0	41.0	34.0	41.0
29	37.96370681806744	40.0	36.0	41.0	34.0	41.0
30	37.910939194166005	40.0	36.0	41.0	33.0	41.0
31	37.84656946395158	40.0	36.0	41.0	33.0	41.0
32	37.72146862414086	40.0	36.0	41.0	33.0	41.0
33	37.65161656319625	40.0	36.0	41.0	33.0	41.0
34	37.56122901807568	40.0	36.0	41.0	33.0	41.0
35	37.49724815972111	40.0	36.0	41.0	33.0	41.0
36	37.44042559356333	40.0	36.0	41.0	33.0	41.0
37	37.286484186640195	40.0	35.0	41.0	33.0	41.0
38	37.216299678380096	40.0	35.0	41.0	32.0	41.0
39	37.1777891643525	40.0	35.0	41.0	32.0	41.0
40	37.12143171943887	40.0	35.0	41.0	32.0	41.0
41	37.01953204227876	39.0	35.0	41.0	32.0	41.0
42	36.936135040709544	39.0	35.0	41.0	32.0	41.0
43	36.865878858028225	39.0	35.0	41.0	32.0	41.0
44	36.84958131463088	39.0	35.0	41.0	32.0	41.0
45	36.779715528797105	39.0	35.0	41.0	31.0	41.0
46	36.69183658841955	39.0	35.0	41.0	31.0	41.0
47	36.53577087365019	39.0	35.0	41.0	31.0	41.0
48	36.53519595329268	39.0	35.0	41.0	31.0	41.0
49	36.42743574845633	39.0	35.0	41.0	31.0	41.0
50	36.30717064459704	39.0	35.0	41.0	31.0	41.0
51	35.531388059041426	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	11.0
11	3.0
12	11.0
13	14.0
14	18.0
15	26.0
16	30.0
17	56.0
18	134.0
19	226.0
20	577.0
21	1191.0
22	1851.0
23	2448.0
24	3227.0
25	3805.0
26	4465.0
27	5163.0
28	5565.0
29	6630.0
30	8049.0
31	10112.0
32	13396.0
33	18410.0
34	35492.0
35	54721.0
36	42227.0
37	65146.0
38	115441.0
39	256798.0
40	495.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.796295194916304	21.04711754452583	22.183690866696253	10.97289639386162
2	31.403156419511912	29.774469571158214	22.22425553913652	16.598118470193356
3	31.044479315829527	28.337626173668646	23.567312193954034	17.050582316547793
4	28.488142458249648	23.920804339504958	29.223491520305974	18.367561681939417
5	29.116284885999544	28.788717531105934	22.405424076200582	19.689573506693932
6	24.677503229161424	37.585608996207355	23.204212625983047	14.532675148648174
7	74.66065211523417	6.890046862871582	13.400371791997777	5.048929229896468
8	73.22091124114174	11.39409799265871	9.489845869494603	5.895144896704959
9	67.27696673849358	9.17005595179819	11.333860979072593	12.21911633063563
10	42.188936824335144	24.203537056438268	18.741183664941904	14.86634245428468
11	32.78433776647254	24.764122529710573	22.788653481623136	19.662886222193755
12	29.035918034961867	21.542433544849125	27.225910150775533	22.195738269413475
13	26.32967488787528	24.556571705683478	29.6245632816515	19.489190124789744
14	19.94455144774706	30.695257135798627	26.95796981439375	22.402221602060564
15	18.876450072665662	25.96474533468142	34.260830843790934	20.89797374886198
16	20.853749105975968	26.16680620303991	29.730092429503628	23.24935226148049
17	21.033240156585737	26.971542204796695	27.95897173130327	24.0362459073143
18	21.609533003021	26.46753377466477	28.335491190908634	23.587442031405597
19	24.437927663734115	26.37527201967844	25.854641223772116	23.332159092815324
20	26.72540919232077	26.44923392243608	28.059620918561084	18.76573596668207
21	24.315013656264725	28.61425893985906	26.862048088961682	20.208679314914534
22	21.544873525146286	24.783184875782126	28.075938286798337	25.596003312273254
23	23.04302142760197	26.926402569299253	28.132362831170138	21.89821317192864
24	23.276192044749237	25.35658024561452	27.794578058782175	23.572649650854068
25	22.10622149226145	29.990560326225367	25.321658027611427	22.58156015390176
26	21.444529335425617	27.18153300912095	27.3456216841049	24.028315971348533
27	24.607048798080953	27.84032768935391	26.084609366779365	21.468014145785773
28	20.255191439329128	28.794817481848835	28.81464232176325	22.135348757058786
29	24.292138840978858	25.602713258090443	27.04687659647148	23.058271304459215
30	23.443025697567492	28.43202291141499	26.044044694339092	22.080906696678422
31	23.93910419173365	28.42211049145778	23.550232331873918	24.088552984934648
32	25.66950771872517	29.885336175910382	23.487707836759217	20.95744826860523
33	24.047835813725804	26.90703522569055	23.1502280619084	25.894900898675242
34	22.899672585143875	27.354619111450674	26.501083503750706	23.24462479965474
35	24.88444405811423	26.8774504645875	24.664388335064196	23.573717142234077
36	25.920673190563985	28.78261758036304	23.24447230088617	22.052236928186804
37	23.807040258149915	29.673820383900402	26.73013665414652	19.789002703803167
38	21.692797330661556	29.390172674355654	23.215345036088834	25.701684958893956
39	23.532542474719516	25.866231130183625	27.66007414490128	22.94115225019558
40	23.053696341402045	25.179224177764763	28.198852294267724	23.56822718656547
41	21.933135389931728	26.536310719290938	26.98450460012535	24.54604929065198
42	22.622124826342027	27.942654363066016	27.57025237021211	21.864968440379844
43	26.47119374511051	25.12722209768156	25.138354507787348	23.26322964942058
44	23.633649158283045	25.601035771636145	26.03885973620763	24.72645533387318
45	22.12192886542441	25.530581340555674	25.363442690200277	26.984047103819638
46	24.549709261097714	28.083410726458386	24.578989024663624	22.787890987780273
47	21.808848893545186	26.266692896454863	30.080382100914537	21.84407610908542
48	22.738633885531375	25.330197958651485	27.443068397222692	24.48809975859445
49	22.21037815119643	24.646240981604073	29.757389709078097	23.385991158121396
50	22.051016938038227	24.87254915416558	27.744405963921842	25.33202794387435
51	21.407624633431084	24.392635529468098	25.4828492259925	28.716890611108315
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	273.0
1	707.5
2	1142.0
3	1950.5
4	2759.0
5	1971.0
6	1183.0
7	1132.5
8	1082.0
9	1082.0
10	1082.0
11	1101.0
12	1120.0
13	1153.0
14	1186.0
15	1182.5
16	1179.0
17	1360.5
18	1542.0
19	1458.0
20	1374.0
21	2008.5
22	2643.0
23	2814.5
24	2986.0
25	3730.0
26	5686.0
27	6898.0
28	7815.5
29	8733.0
30	9521.5
31	10310.0
32	11930.0
33	13550.0
34	14286.5
35	15023.0
36	15925.5
37	16828.0
38	18041.5
39	19255.0
40	22989.0
41	26723.0
42	29678.0
43	32633.0
44	37337.0
45	42041.0
46	59159.5
47	76278.0
48	69007.5
49	61737.0
50	60154.5
51	58572.0
52	51527.0
53	44482.0
54	40313.0
55	36144.0
56	33388.0
57	30632.0
58	29896.0
59	29160.0
60	28387.0
61	27614.0
62	24977.0
63	22340.0
64	20251.0
65	18162.0
66	14976.5
67	11791.0
68	10107.0
69	8423.0
70	7209.0
71	5995.0
72	4696.5
73	3398.0
74	2754.0
75	1727.0
76	1344.0
77	1116.5
78	889.0
79	623.0
80	357.0
81	267.0
82	177.0
83	120.0
84	63.0
85	58.0
86	53.0
87	26.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	655743.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.385249675311716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.69198021244165	18.916107976227803
2	11.044358505870786	5.828163133621067
3	3.991020785492637	3.1591223965374575
4	1.9771867291962064	2.0867426201821937
5	1.2164778038479902	1.6048535289502046
6	0.8233780489368928	1.3035021239022568
7	0.6431379897722351	1.1878549505072948
8	0.48404176947168376	1.0217250352632041
9	0.4082930898808526	0.9695623605489706
>10	7.211485655416807	47.344023166482984
>50	0.41637187037465034	6.6350966938888325
>100	0.07883618864036146	3.674249123646671
>500	0.007591632980182955	1.544522067241224
>1k	0.0040878023739446675	1.6382041718783615
>5k	0.0017519153031191434	3.08627065112148
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	6890	1.0507165154641376	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	6632	1.011371833172447	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	6508	0.9924619858694641	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	2607	0.3975642896683609	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	1541	0.23500060237013587	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAACTAGT	1493	0.22768066147865856	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1481	0.22585067625578922	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1454	0.22173320950433323	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	1029	0.15692123286104465	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA	1027	0.15661623532389976	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	980	0.1494487932009949	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	973	0.1483813018209878	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	957	0.1459413215238287	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	933	0.14228135107809003	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	920	0.14029886708664827	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	865	0.13191143481516387	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	670	0.10217417494353734	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1459413215238287	0.0
2	0.0	0.0	0.0	0.8825103737287321	0.0
3	0.0	0.0	0.0	1.1724105327849477	0.0
4	0.0	0.0	0.0	1.750838361980227	0.0
5	0.0	0.0	0.0	3.940263182374802	0.0
6	0.0	0.0	0.0	4.668749799845366	0.0
7	0.0	0.0	0.0	5.539365269625447	0.0
8	0.0	0.0	0.0	6.605026664409685	0.0
9	0.0	0.0	0.0	7.018755823546725	0.0
10	0.0	0.0	0.0	8.9978848420799	0.0
11	0.0	0.0	0.0	10.323861634817298	0.0
12	1.5249876857244377E-4	0.0	0.0	12.342182836873592	0.0
13	1.5249876857244377E-4	0.0	0.0	12.816606505902465	0.0
14	1.5249876857244377E-4	0.0	0.0	13.027817300375299	0.0
15	1.5249876857244377E-4	0.0	0.0	13.539145671398703	0.0
16	1.5249876857244377E-4	0.0	0.0	14.244299977277683	0.0
17	1.5249876857244377E-4	0.0	0.0	15.008166309057055	0.0
18	1.5249876857244377E-4	0.0	0.0	15.830287170431099	0.0
19	1.5249876857244377E-4	0.0	0.0	16.557706296521655	0.0
20	1.5249876857244377E-4	0.0	0.0	17.10288939416814	0.0
21	1.5249876857244377E-4	0.0	0.0	17.703887041112143	0.0
22	1.5249876857244377E-4	0.0	0.0	18.392418981216725	0.0
23	1.5249876857244377E-4	0.0	0.0	18.981979220517793	0.0
24	1.5249876857244377E-4	0.0	0.0	19.47409274670107	0.0
25	1.5249876857244377E-4	0.0	0.0	19.92030414354404	0.0
26	1.5249876857244377E-4	0.0	0.0	20.332203317458212	0.0
27	1.5249876857244377E-4	0.0	0.0	20.81013445816425	0.0
28	1.5249876857244377E-4	0.0	0.0	21.23133605696134	0.0
29	1.5249876857244377E-4	0.0	0.0	21.670227512912835	0.0
30	1.5249876857244377E-4	0.0	0.0	22.168745987376152	0.0
31	1.5249876857244377E-4	0.0	0.0	22.636917206893553	0.0
32	1.5249876857244377E-4	0.0	0.0	23.128573236771114	0.0
33	1.5249876857244377E-4	0.0	0.0	23.584849552339865	0.0
34	1.5249876857244377E-4	0.0	0.0	24.015506074788448	0.0
35	3.0499753714488754E-4	0.0	0.0	24.4762048546458	0.0
36	3.0499753714488754E-4	0.0	0.0	24.928211204694524	0.0
37	3.0499753714488754E-4	0.0	0.0	25.392874952534758	0.0
38	3.0499753714488754E-4	0.0	0.0	25.80675661044037	0.0
39	3.0499753714488754E-4	0.0	0.0	26.25357800235763	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	20	7.0310675E-4	45.0	1
CTCGTAG	20	7.0310675E-4	45.0	1
CGCATCG	45	3.8380676E-10	45.0	21
TACGCGG	35	1.2108649E-7	45.0	2
TACGCAT	20	7.0310675E-4	45.0	27
CAAGCGT	35	1.2108649E-7	45.0	17
GACGAAT	35	1.2108649E-7	45.0	20
CGTCCAC	20	7.0310675E-4	45.0	11
ATTTCGA	20	7.0310675E-4	45.0	11
CCCGATC	35	1.2108649E-7	45.0	41
CTCGTGA	25	3.8889677E-5	44.999996	12
ACGGGTA	25	3.8889677E-5	44.999996	5
TATAGCG	25	3.8889677E-5	44.999996	1
CGACTTG	25	3.8889677E-5	44.999996	41
CGTAAGG	65	0.0	41.538464	2
TACGGCT	755	0.0	41.42384	7
TAACGCC	50	1.0804797E-9	40.499996	12
CCGCATC	50	1.0804797E-9	40.499996	20
TGCAACG	50	1.0804797E-9	40.499996	1
GATTGCG	45	1.9261279E-8	40.0	1
>>END_MODULE