FastQCFastQC Report
Sat 18 Jun 2016
SRR3551209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692799
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC62270.898817694598289No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG62200.8978073005301682No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC57380.8282344518395667No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC35970.5191982090043432TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14570.21030630817885132No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC14150.20424394377012667TruSeq Adapter, Index 23 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT14140.20409960176039516No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACTACT13880.20034670950737515No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT12270.1771076459405975TruSeq Adapter, Index 20 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT10180.14694016590670597No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC10160.1466514818872429No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTA8990.12976346674865294No Hit
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC7720.11143203151274755TruSeq Adapter, Index 23 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG253.8891954E-545.01
TCGCATA207.0313446E-445.020
CGCATAA207.0313446E-445.021
CGTAGTA207.0313446E-445.042
ATAGTCG207.0313446E-445.015
TACCGGT207.0313446E-445.040
AAATCGG302.1642481E-644.9999962
TTCGATC302.1642481E-644.99999636
CGTAAGG1250.043.1999972
AACACGT1500.042.041
CGATGAA6100.041.6803319
CCGATGA5700.041.4473718
ACGCCGG900.040.00000427
CGGTAGT850.039.70588312
CGTTAGG800.039.3752
CGAATAT800.039.37514
TACGGCT6650.039.2481237
AACGGGC700.038.571434
GTATGCG356.2446943E-638.571431
ACCGTTC356.2446943E-638.5714332
Produced by FastQC (version 0.11.5)