##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551209_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692799
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25682052081484	33.0	31.0	34.0	30.0	34.0
2	32.35927736616248	34.0	31.0	34.0	30.0	34.0
3	32.39223786408468	34.0	31.0	34.0	30.0	34.0
4	35.91824035542776	37.0	35.0	37.0	35.0	37.0
5	35.9480080080947	37.0	35.0	37.0	35.0	37.0
6	35.96907905467531	37.0	35.0	37.0	35.0	37.0
7	36.107709451081774	37.0	35.0	37.0	35.0	37.0
8	35.66880581525089	37.0	35.0	37.0	35.0	37.0
9	37.602331989509224	39.0	37.0	39.0	35.0	39.0
10	37.50095482239438	39.0	37.0	39.0	35.0	39.0
11	37.43783406153877	39.0	37.0	39.0	35.0	39.0
12	37.26663289063639	39.0	37.0	39.0	35.0	39.0
13	37.16607991639711	39.0	37.0	39.0	34.0	39.0
14	38.37929038581176	40.0	38.0	41.0	34.0	41.0
15	38.41228841265649	40.0	38.0	41.0	34.0	41.0
16	38.525482860108056	40.0	38.0	41.0	34.0	41.0
17	38.46177318385275	40.0	38.0	41.0	34.0	41.0
18	38.477959696824044	40.0	38.0	41.0	34.0	41.0
19	38.474979034323084	40.0	37.0	41.0	34.0	41.0
20	38.344014642053466	40.0	37.0	41.0	34.0	41.0
21	38.35672395601033	40.0	37.0	41.0	34.0	41.0
22	38.31151170830212	40.0	37.0	41.0	34.0	41.0
23	38.25336064284158	40.0	37.0	41.0	34.0	41.0
24	38.20832882264553	40.0	37.0	41.0	34.0	41.0
25	38.16609146375789	40.0	37.0	41.0	34.0	41.0
26	38.130961505429426	40.0	37.0	41.0	34.0	41.0
27	38.053732756542665	40.0	36.0	41.0	34.0	41.0
28	37.986208120970154	40.0	36.0	41.0	34.0	41.0
29	37.965041808663116	40.0	36.0	41.0	34.0	41.0
30	37.884807859133744	40.0	36.0	41.0	34.0	41.0
31	37.87938348640804	40.0	36.0	41.0	34.0	41.0
32	37.713593697450484	40.0	36.0	41.0	33.0	41.0
33	37.678530136446504	40.0	36.0	41.0	33.0	41.0
34	37.59847805784939	40.0	36.0	41.0	33.0	41.0
35	37.516395087175354	40.0	36.0	41.0	33.0	41.0
36	37.50555500224452	40.0	35.0	41.0	33.0	41.0
37	37.427639185391435	40.0	35.0	41.0	33.0	41.0
38	37.39681350579317	40.0	35.0	41.0	33.0	41.0
39	37.31473486537942	40.0	35.0	41.0	33.0	41.0
40	37.22559934410991	40.0	35.0	41.0	33.0	41.0
41	37.14826811239624	40.0	35.0	41.0	32.0	41.0
42	37.09759107619959	40.0	35.0	41.0	33.0	41.0
43	37.05848449550302	39.0	35.0	41.0	33.0	41.0
44	37.043457048869875	39.0	35.0	41.0	32.0	41.0
45	36.96461455631432	39.0	35.0	41.0	32.0	41.0
46	36.84836438851673	39.0	35.0	41.0	32.0	41.0
47	36.74532007118948	39.0	35.0	41.0	32.0	41.0
48	36.75520605543599	39.0	35.0	41.0	32.0	41.0
49	36.69584973419419	39.0	35.0	41.0	32.0	41.0
50	36.602864611525135	39.0	35.0	41.0	32.0	41.0
51	35.89319846015944	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	14.0
11	7.0
12	9.0
13	9.0
14	16.0
15	23.0
16	45.0
17	78.0
18	119.0
19	246.0
20	504.0
21	884.0
22	1546.0
23	2213.0
24	3160.0
25	3836.0
26	4652.0
27	5233.0
28	5882.0
29	6676.0
30	8157.0
31	10688.0
32	14013.0
33	19774.0
34	38473.0
35	59430.0
36	44279.0
37	66603.0
38	118379.0
39	277297.0
40	538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.14556314313386	20.31570484368482	22.49238235043642	11.046349662744895
2	31.209918028172673	27.800704100323472	22.587792418868965	18.40158545263489
3	30.748600965070676	27.733007697759376	24.816288707114182	16.70210263005576
4	28.614648693199612	23.64870619039577	28.741814003773097	18.994831112631513
5	28.344584792991906	27.602378178952335	23.3193177241884	20.733719303867357
6	24.464671571408157	36.35195778284899	23.600928985174633	15.582441660568216
7	75.73524211207003	6.289847416061513	12.749441035567315	5.225469436301149
8	74.63362389379893	9.435204150121464	9.714794622971453	6.21637733310816
9	68.88274954207498	8.063089005613461	11.398544166489847	11.655617285821718
10	42.00006062364409	24.28554313733132	18.907648538753666	14.80674770027093
11	32.628799983833694	23.870704201362877	24.173678079789376	19.32681773501405
12	30.15116938679184	20.514896817114344	27.515917315123144	21.81801648097067
13	26.248305785660776	22.91645917502768	30.403912245831766	20.431322793479783
14	20.23314121411838	28.852957351266383	27.868400502887564	23.045500931727673
15	19.877626844149603	24.98358109639304	33.93177530568029	21.20701675377707
16	22.299685767444817	25.029337513477934	29.462946684391866	23.208030034685383
17	21.17121993536365	25.77298754761482	28.761300175086856	24.294492341934674
18	22.810945165913925	25.25407802262994	28.68653101404592	23.248445797410216
19	23.63167383324745	27.265339586229192	25.764327027030927	23.338659553492427
20	27.21972751115403	26.439558948555064	27.709479950173137	18.63123359011777
21	24.69172155271587	28.670220366946257	26.889184308868806	19.748873771469068
22	22.43349081046595	23.83173185873536	28.722616516478805	25.012160814319884
23	23.51634456747195	26.77241162299599	27.536269538495294	22.174974271036767
24	24.723188110837345	23.826246862365565	27.786991609398974	23.66357341739812
25	22.3412562662475	27.360027944613087	25.563114265465163	24.735601523674255
26	20.64595936195058	25.451249207923222	28.73719505946169	25.165596370664506
27	23.028612916589083	25.80762962995039	27.212654752677185	23.951102700783345
28	19.244687131476805	27.45904656328892	28.664735370576462	24.631530934657818
29	22.182047029513612	24.631530934657818	27.04651709947618	26.139904936352394
30	21.512444446369006	25.703992066963146	28.970307405178126	23.813256081489726
31	23.306615627332025	25.28973049903363	24.99512845717156	26.408525416462787
32	24.28207892909776	27.547816899273812	24.92656600254908	23.243538169079343
33	22.619547661009904	24.813401866919556	24.959764664787336	27.607285807283212
34	20.551415345576423	25.126624028036993	28.429313552704322	25.89264707368227
35	19.570034021411693	27.212654752677185	27.39798989317248	25.819321332738642
36	20.318014315840525	27.46655234779496	25.168194526839677	27.047238809524842
37	21.373731775016996	27.560085970100996	28.262887215483858	22.80329503939815
38	19.972603886552953	28.325098621678148	23.493105503905173	28.209191987863726
39	22.800985567242446	25.140913887000416	26.55589860839868	25.502201937358453
40	21.893651694069995	24.383840045958497	28.430179604762706	25.292328655208795
41	18.83591055991709	26.389905297207417	27.35454294824329	27.41964119463221
42	20.761721653755274	25.238633427588663	27.61609066987683	26.383554248779227
43	22.07119236603979	24.13932468147327	26.00191397504904	27.7875689774379
44	21.189840054619015	23.8126787134508	27.280351155241274	27.71713007668891
45	21.03250726401164	23.34717573206659	27.324086784189934	28.296230219731843
46	23.52991271638672	25.711209167449724	25.212940549856448	25.54593756630711
47	19.015905046052318	24.860457362092035	30.994415407643487	25.129222184212157
48	20.97058454183681	23.23184646629109	28.394382786349286	27.40318620552281
49	20.900145641087818	22.312965232340115	30.594299356667666	26.1925897699044
50	19.876905134100944	22.833318177422314	29.480845093598578	27.808931594878167
51	19.28827841841573	22.99671333243841	26.562827024865797	31.152181224280056
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	306.0
1	689.0
2	1072.0
3	1991.0
4	2910.0
5	2083.5
6	1257.0
7	1212.0
8	1167.0
9	1143.0
10	1119.0
11	1097.5
12	1076.0
13	1145.0
14	1214.0
15	1179.5
16	1145.0
17	1269.5
18	1394.0
19	1310.5
20	1227.0
21	1521.5
22	1816.0
23	2055.5
24	2295.0
25	2728.5
26	4086.0
27	5010.0
28	5961.5
29	6913.0
30	7706.0
31	8499.0
32	9995.5
33	11492.0
34	13108.0
35	14724.0
36	16134.5
37	17545.0
38	19407.0
39	21269.0
40	23609.5
41	25950.0
42	29516.5
43	33083.0
44	38080.5
45	43078.0
46	60224.5
47	77371.0
48	73513.5
49	69656.0
50	67261.0
51	64866.0
52	57373.5
53	49881.0
54	45997.0
55	42113.0
56	39088.0
57	36063.0
58	35298.0
59	34533.0
60	32694.0
61	30855.0
62	28653.0
63	26451.0
64	22351.5
65	18252.0
66	14861.0
67	11470.0
68	9430.0
69	7390.0
70	6458.5
71	5527.0
72	4612.0
73	3697.0
74	2974.5
75	2004.0
76	1756.0
77	1375.5
78	995.0
79	792.0
80	589.0
81	419.0
82	249.0
83	164.0
84	79.0
85	50.0
86	21.0
87	13.0
88	5.0
89	3.0
90	1.0
91	2.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	692799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.360727353800367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.44096949933903	19.546768883625834
2	11.253609472072652	6.158138810230502
3	4.168245671241043	3.4213870006345433
4	1.9869510183869479	2.174577003177651
5	1.2392541961742831	1.6953448091788799
6	0.827664003016817	1.358729347625888
7	0.5970138107105808	1.143431247091391
8	0.5063401031515756	1.1083066808500333
9	0.44057936773007156	1.0849114762355014
>10	7.009842782181021	46.94508100822948
>50	0.46066981409728525	7.569421139798142
>100	0.059785653741464787	2.7570178257653555
>500	0.003202802879007042	0.5506148860060068
>1k	0.0042704038386760556	1.830319827964795
>5k	0.001601401439503521	2.655950053586004
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	6227	0.898817694598289	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	6220	0.8978073005301682	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	5738	0.8282344518395667	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	3597	0.5191982090043432	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1457	0.21030630817885132	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1415	0.20424394377012667	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	1414	0.20409960176039516	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACTACT	1388	0.20034670950737515	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	1227	0.1771076459405975	TruSeq Adapter, Index 20 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	1018	0.14694016590670597	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC	1016	0.1466514818872429	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTA	899	0.12976346674865294	No Hit
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	772	0.11143203151274755	TruSeq Adapter, Index 23 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.443420097315383E-4	0.0	0.0	0.18259264231039596	0.0
2	1.443420097315383E-4	0.0	0.0	1.127022411983851	0.0
3	1.443420097315383E-4	0.0	0.0	1.4784952056801468	0.0
4	1.443420097315383E-4	0.0	0.0	2.065822843277776	0.0
5	1.443420097315383E-4	0.0	0.0	4.237015353659575	0.0
6	1.443420097315383E-4	0.0	0.0	5.093396497396792	0.0
7	1.443420097315383E-4	0.0	0.0	6.045187709566555	0.0
8	1.443420097315383E-4	0.0	0.0	7.382516429729257	0.0
9	1.443420097315383E-4	0.0	0.0	7.892476750110783	0.0
10	1.443420097315383E-4	0.0	0.0	9.828536126639905	0.0
11	1.443420097315383E-4	0.0	0.0	11.186938780223413	0.0
12	1.443420097315383E-4	0.0	0.0	13.072767137365961	0.0
13	1.443420097315383E-4	0.0	0.0	13.572479175056547	0.0
14	1.443420097315383E-4	0.0	0.0	13.78480627137164	0.0
15	1.443420097315383E-4	0.0	0.0	14.287260807247124	0.0
16	1.443420097315383E-4	0.0	0.0	15.009259539924278	0.0
17	1.443420097315383E-4	0.0	0.0	15.832008995394046	0.0
18	1.443420097315383E-4	0.0	0.0	16.715815120980256	0.0
19	1.443420097315383E-4	0.0	0.0	17.49699407764734	0.0
20	1.443420097315383E-4	0.0	0.0	18.039142666198998	0.0
21	1.443420097315383E-4	0.0	0.0	18.67742303323186	0.0
22	1.443420097315383E-4	0.0	0.0	19.41212386276539	0.0
23	2.886840194630766E-4	0.0	0.0	20.054301464061005	0.0
24	2.886840194630766E-4	0.0	0.0	20.608719123439844	0.0
25	2.886840194630766E-4	0.0	0.0	21.086346833641503	0.0
26	2.886840194630766E-4	0.0	0.0	21.523558781118332	0.0
27	2.886840194630766E-4	0.0	0.0	21.990938208629053	0.0
28	2.886840194630766E-4	0.0	0.0	22.446770275361253	0.0
29	2.886840194630766E-4	0.0	0.0	22.887157747052175	0.0
30	2.886840194630766E-4	0.0	0.0	23.41602687070853	0.0
31	2.886840194630766E-4	0.0	0.0	23.88788090052093	0.0
32	2.886840194630766E-4	0.0	0.0	24.355404670041384	0.0
33	4.3302602919461486E-4	0.0	0.0	24.825526595737003	0.0
34	4.3302602919461486E-4	0.0	0.0	25.28106997844974	0.0
35	5.773680389261532E-4	0.0	0.0	25.73358217895811	0.0
36	5.773680389261532E-4	0.0	0.0	26.185950037456752	0.0
37	7.217100486576915E-4	0.0	0.0	26.648999204675526	0.0
38	7.217100486576915E-4	0.0	0.0	27.14755650628826	0.0
39	7.217100486576915E-4	0.0	0.0	27.637020261287905	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	25	3.8891954E-5	45.0	1
TCGCATA	20	7.0313446E-4	45.0	20
CGCATAA	20	7.0313446E-4	45.0	21
CGTAGTA	20	7.0313446E-4	45.0	42
ATAGTCG	20	7.0313446E-4	45.0	15
TACCGGT	20	7.0313446E-4	45.0	40
AAATCGG	30	2.1642481E-6	44.999996	2
TTCGATC	30	2.1642481E-6	44.999996	36
CGTAAGG	125	0.0	43.199997	2
AACACGT	150	0.0	42.0	41
CGATGAA	610	0.0	41.68033	19
CCGATGA	570	0.0	41.44737	18
ACGCCGG	90	0.0	40.000004	27
CGGTAGT	85	0.0	39.705883	12
CGTTAGG	80	0.0	39.375	2
CGAATAT	80	0.0	39.375	14
TACGGCT	665	0.0	39.248123	7
AACGGGC	70	0.0	38.57143	4
GTATGCG	35	6.2446943E-6	38.57143	1
ACCGTTC	35	6.2446943E-6	38.57143	32
>>END_MODULE