FastQCFastQC Report
Sat 18 Jun 2016
SRR3551208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598844
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.2519854920480125No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC14680.2451389677445211Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT10740.17934553907194528Illumina PCR Primer Index 7 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC10090.16849129322494674Illumina PCR Primer Index 7 (96% over 27bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC8960.14962160429093388No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8750.1461148479403651No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC7500.12524129823459865Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG7380.12323743746284507No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7060.11789380873816888No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC7020.11722585514758435No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC6800.11355211039936945No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6350.10603763250529354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAACG351.2106284E-745.0000041
TAGCCGT253.8885588E-545.00000444
TATTGCG253.8885588E-545.0000041
TACGATC253.8885588E-545.00000410
TACGACG351.2106284E-745.0000041
TATAGCG600.045.0000041
CGGTCAT253.8885588E-545.00000413
ATACGAT253.8885588E-545.0000049
TCGACAA253.8885588E-545.00000419
CGTTTAT253.8885588E-545.00000439
TGTAACG302.1637534E-645.0000041
TAGTACG502.1827873E-1145.0000041
GTACGAG302.1637534E-645.0000041
CGGGTCC406.8048394E-945.06
AACCCGT207.0305774E-445.029
CGTTGAT453.8380676E-1045.025
ACGGGTA1100.045.05
CCCGTTC207.0305774E-445.031
ACTAGTC207.0305774E-445.012
TAACCGG207.0305774E-445.02
Produced by FastQC (version 0.11.5)