##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551208_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	598844
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.356236348698495	33.0	31.0	34.0	30.0	34.0
2	32.460627475603	34.0	31.0	34.0	31.0	34.0
3	32.473719365978454	34.0	31.0	34.0	30.0	34.0
4	35.962061905938775	37.0	35.0	37.0	35.0	37.0
5	36.00176005771119	37.0	35.0	37.0	35.0	37.0
6	36.0514474554308	37.0	35.0	37.0	35.0	37.0
7	36.18253334758301	37.0	37.0	37.0	35.0	37.0
8	35.83484847472798	37.0	35.0	37.0	35.0	37.0
9	37.735735851073066	39.0	38.0	39.0	35.0	39.0
10	37.62529640440582	39.0	37.0	39.0	35.0	39.0
11	37.56656157530175	39.0	37.0	39.0	35.0	39.0
12	37.36394453313384	39.0	37.0	39.0	35.0	39.0
13	37.27592160896661	39.0	37.0	39.0	34.0	39.0
14	38.52102216937967	40.0	38.0	41.0	35.0	41.0
15	38.59392596402402	40.0	38.0	41.0	35.0	41.0
16	38.687546339280345	40.0	38.0	41.0	35.0	41.0
17	38.678906359586136	40.0	38.0	41.0	35.0	41.0
18	38.6835202490131	40.0	38.0	41.0	35.0	41.0
19	38.68392102116745	40.0	38.0	41.0	35.0	41.0
20	38.59422988290773	40.0	38.0	41.0	35.0	41.0
21	38.519430769950105	40.0	37.0	41.0	35.0	41.0
22	38.50903407231266	40.0	37.0	41.0	35.0	41.0
23	38.47417858407198	40.0	37.0	41.0	35.0	41.0
24	38.43719566364529	40.0	37.0	41.0	35.0	41.0
25	38.34631222822638	40.0	37.0	41.0	34.0	41.0
26	38.310563352058296	40.0	37.0	41.0	34.0	41.0
27	38.27403797984116	40.0	37.0	41.0	34.0	41.0
28	38.197817795619564	40.0	37.0	41.0	34.0	41.0
29	38.17106959408461	40.0	36.0	41.0	34.0	41.0
30	38.090569497231336	40.0	36.0	41.0	34.0	41.0
31	38.03077596168618	40.0	36.0	41.0	34.0	41.0
32	37.954849677044436	40.0	36.0	41.0	34.0	41.0
33	37.901740687057064	40.0	36.0	41.0	34.0	41.0
34	37.7985602260355	40.0	36.0	41.0	34.0	41.0
35	37.73841935462324	40.0	36.0	41.0	33.0	41.0
36	37.69163087548677	40.0	36.0	41.0	33.0	41.0
37	37.612510102798055	40.0	35.0	41.0	33.0	41.0
38	37.54014234091015	40.0	35.0	41.0	33.0	41.0
39	37.49723467213498	40.0	35.0	41.0	33.0	41.0
40	37.40925683483511	40.0	35.0	41.0	33.0	41.0
41	37.32292550313604	40.0	35.0	41.0	33.0	41.0
42	37.3023692313858	40.0	35.0	41.0	33.0	41.0
43	37.21667913513369	40.0	35.0	41.0	33.0	41.0
44	37.15160876622292	39.0	35.0	41.0	33.0	41.0
45	37.07153949943558	39.0	35.0	41.0	33.0	41.0
46	36.92634141779829	39.0	35.0	41.0	32.0	41.0
47	36.87558028468182	39.0	35.0	41.0	32.0	41.0
48	36.83730988370928	39.0	35.0	41.0	32.0	41.0
49	36.74912164102838	39.0	35.0	41.0	32.0	41.0
50	36.681422874738665	39.0	35.0	41.0	32.0	41.0
51	35.864612486724425	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	16.0
11	10.0
12	14.0
13	13.0
14	18.0
15	24.0
16	36.0
17	66.0
18	108.0
19	221.0
20	322.0
21	561.0
22	834.0
23	1204.0
24	1822.0
25	2591.0
26	3554.0
27	4435.0
28	4872.0
29	5540.0
30	6502.0
31	8475.0
32	11153.0
33	15497.0
34	31958.0
35	53964.0
36	37419.0
37	56723.0
38	101368.0
39	248944.0
40	568.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.9565763370761	22.06935362131039	25.120231646305214	11.853838395308294
2	33.071885165418706	24.61793054618565	25.194875460053034	17.115308828342606
3	32.70183887623488	24.141679636098885	25.77349025789688	17.382991229769356
4	29.56345893087348	25.254490318012706	25.939476725157135	19.242574025956678
5	26.285309696682273	29.79240002404633	24.170902605686955	19.75138767358444
6	25.36303277648269	35.01212335766911	23.66659764479564	15.95824622105256
7	78.29818784190874	7.429480799674039	9.117900488274074	5.154430870143142
8	78.77026404205436	7.224586035762235	8.840365771386203	5.164784150797202
9	72.68370393625051	9.099865741328292	11.298935949930199	6.917494372490999
10	40.703421926244566	27.33650165986467	18.092190954572477	13.867885459318286
11	32.395916131747164	25.32512641021702	23.325774325199884	18.953183132835928
12	30.04021080615319	22.5547888932677	27.28356633781085	20.12143396276827
13	25.24730981691392	26.0617122322341	28.264623173981875	20.426354776870102
14	19.696949455951803	29.900942482516314	28.494900174335886	21.907207887195998
15	19.628484212916884	25.660773089485744	34.66545544415574	20.045287253441632
16	21.98869822524731	25.824588707576595	31.109604504679016	21.077108562497077
17	21.46552357542198	25.668788532572755	27.84681820307125	25.018869688934014
18	23.52064978525292	25.457715197948044	29.025923278850584	21.995711737948447
19	25.063121614310234	26.736512347122122	26.85974978458497	21.340616253982674
20	27.055293198228586	26.22202109397439	27.178697624089075	19.543988083707944
21	24.867578200666618	26.62429614390392	28.3280787650874	20.18004689034206
22	23.319929731282272	25.51883295148653	27.10305187995538	24.058185437275817
23	22.802098710181617	25.734247984450043	27.71105663578495	23.752596669583397
24	23.042562002792046	24.388488487819863	29.17721476711798	23.391734742270106
25	21.55285850739091	27.322307646064754	27.520522874070707	23.604310972473634
26	20.032262158425233	28.788966742590727	27.33366285710469	23.845108241879352
27	23.037385362465017	27.97673517643994	27.746124199290634	21.23975526180441
28	20.104735123003653	27.886060476518093	29.557781325353517	22.45142307512474
29	22.251036997949384	26.78176620288422	28.732858640981622	22.23433815818477
30	23.425299410196978	27.69485876121327	26.81816967357108	22.06167215501867
31	24.50571434296745	28.77377079840493	24.017607256647807	22.702907601979813
32	24.738329180888513	27.964211046616484	25.293899579857193	22.003560192637817
33	24.123978865948395	28.128193653104983	24.12765261069661	23.620174870250015
34	21.58625618692013	29.286091202383258	26.427082846283838	22.700569764412766
35	22.736138293111395	27.719740032462543	26.116818403457327	23.427303270968732
36	22.997308147029944	29.610549659009695	24.845201755382035	22.54694043857833
37	23.718531036463585	29.530228239741902	26.404372424203963	20.346868299590547
38	22.21880823720368	27.930479390291964	25.651922704410495	24.198789668093863
39	22.843344844400214	26.48903554181056	26.279298114367013	24.38832149942222
40	23.009331311660468	26.078578060396364	27.95519367314359	22.95689695479958
41	19.284989078958795	26.810154230484066	27.487960136529715	26.416896554027424
42	22.237176960944755	26.18545063488989	27.32013679689535	24.257235607270005
43	23.505620829464767	25.65075378562697	26.20699213818624	24.636633246722017
44	22.19542986153322	26.612606956068692	26.056535591907075	25.135427590491012
45	22.81829658475329	26.695767178096464	25.405948794677748	25.079987442472497
46	22.797089058252233	27.44888485148052	26.022803935582555	23.73122215468469
47	21.70381601886301	26.713133971451665	28.275310431431222	23.307739578254104
48	21.743225280707495	24.78375002504826	29.008890462290683	24.46413423195356
49	22.642457802031917	24.63312649037145	28.704470613381783	24.019945094214854
50	20.83530936270548	25.560747039295713	28.282657920927655	25.32128567707116
51	20.7847118782187	25.101361957371203	26.1612373172312	27.952688847178898
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	330.0
1	835.0
2	1340.0
3	2165.0
4	2990.0
5	2110.0
6	1230.0
7	1175.0
8	1120.0
9	1161.5
10	1203.0
11	1253.5
12	1304.0
13	1300.5
14	1297.0
15	1263.5
16	1230.0
17	1186.5
18	1143.0
19	1228.5
20	1314.0
21	1752.5
22	2191.0
23	2418.5
24	2646.0
25	3222.5
26	4684.5
27	5570.0
28	6875.5
29	8181.0
30	9215.5
31	10250.0
32	11895.0
33	13540.0
34	14521.5
35	15503.0
36	16495.5
37	17488.0
38	18387.5
39	19287.0
40	22460.0
41	25633.0
42	29828.5
43	34024.0
44	38045.5
45	42067.0
46	46921.0
47	51775.0
48	54490.0
49	57205.0
50	55756.5
51	54308.0
52	48162.0
53	42016.0
54	37261.5
55	32507.0
56	30227.0
57	27947.0
58	26806.5
59	25666.0
60	24998.0
61	24330.0
62	21854.5
63	19379.0
64	17238.0
65	15097.0
66	12785.5
67	10474.0
68	9367.0
69	8260.0
70	6993.0
71	5726.0
72	4710.0
73	3694.0
74	3022.5
75	1910.0
76	1469.0
77	1235.5
78	1002.0
79	744.0
80	486.0
81	375.0
82	264.0
83	197.5
84	131.0
85	85.5
86	40.0
87	35.0
88	30.0
89	18.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	598844.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.183407534474878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27819180834052	18.663059444382483
2	11.54934191761477	6.048022523678021
3	4.277770561100653	3.36019829820833
4	2.1430911953945952	2.244537206102464
5	1.2337225537905085	1.6151530205184994
6	0.8198447929674764	1.2879798197570769
7	0.6026176907951191	1.1045009209900938
8	0.43898776710718174	0.9195356487053186
9	0.3649104350016883	0.8599138769908539
>10	6.163528756357615	41.75875553127459
>50	1.0460388314645341	16.74505287784253
>100	0.073564729664995	3.4280851403919788
>500	0.0058077418156575	1.1102541996902604
>1k	0.0025812185847366668	0.8549514914674659
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1509	0.2519854920480125	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1468	0.2451389677445211	Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	1074	0.17934553907194528	Illumina PCR Primer Index 7 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1009	0.16849129322494674	Illumina PCR Primer Index 7 (96% over 27bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	896	0.14962160429093388	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	875	0.1461148479403651	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	750	0.12524129823459865	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	738	0.12323743746284507	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	706	0.11789380873816888	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	702	0.11722585514758435	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	680	0.11355211039936945	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	635	0.10603763250529354	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.009651929383947E-4	0.0	0.0	0.1858580865801444	0.0
2	5.009651929383947E-4	0.0	0.0	0.7951987495908784	0.0
3	5.009651929383947E-4	0.0	0.0	1.082919758735163	0.0
4	5.009651929383947E-4	0.0	0.0	1.5095751147210292	0.0
5	5.009651929383947E-4	0.0	0.0	2.7032081810955777	0.0
6	5.009651929383947E-4	0.0	0.0	3.407231265571668	0.0
7	5.009651929383947E-4	0.0	0.0	4.081697403664394	0.0
8	5.009651929383947E-4	0.0	0.0	5.108008095597518	0.0
9	5.009651929383947E-4	0.0	0.0	5.565556305147918	0.0
10	5.009651929383947E-4	0.0	0.0	6.655322588186573	0.0
11	5.009651929383947E-4	0.0	0.0	8.086079179218627	0.0
12	5.009651929383947E-4	0.0	0.0	9.368550073140918	0.0
13	5.009651929383947E-4	0.0	0.0	9.855321252279392	0.0
14	5.009651929383947E-4	0.0	0.0	10.072406169219363	0.0
15	5.009651929383947E-4	0.0	0.0	10.45197079706902	0.0
16	5.009651929383947E-4	0.0	0.0	11.188890595881398	0.0
17	5.009651929383947E-4	0.0	0.0	12.015316175832103	0.0
18	5.009651929383947E-4	0.0	0.0	12.99320023244785	0.0
19	5.009651929383947E-4	0.0	0.0	13.548269666223591	0.0
20	5.009651929383947E-4	0.0	0.0	14.112857438665161	0.0
21	5.009651929383947E-4	0.0	0.0	14.76344423589449	0.0
22	5.009651929383947E-4	0.0	0.0	15.46362658722472	0.0
23	5.009651929383947E-4	0.0	0.0	16.071297366258992	0.0
24	5.009651929383947E-4	0.0	0.0	16.63254537074764	0.0
25	5.009651929383947E-4	0.0	0.0	17.1134719559685	0.0
26	5.009651929383947E-4	0.0	0.0	17.555323256140163	0.0
27	5.009651929383947E-4	0.0	0.0	18.012537488895273	0.0
28	5.009651929383947E-4	0.0	0.0	18.445371415594046	0.0
29	5.009651929383947E-4	0.0	0.0	18.906426381495013	0.0
30	5.009651929383947E-4	0.0	0.0	19.43277381087562	0.0
31	5.009651929383947E-4	0.0	0.0	19.922550781171726	0.0
32	5.009651929383947E-4	0.0	0.0	20.402809412802	0.0
33	5.009651929383947E-4	0.0	0.0	20.858854726773583	0.0
34	5.009651929383947E-4	0.0	0.0	21.336608532439165	0.0
35	6.679535905845262E-4	0.0	0.0	21.822210792794117	0.0
36	6.679535905845262E-4	0.0	0.0	22.306978111160838	0.0
37	6.679535905845262E-4	0.0	0.0	22.791912417925204	0.0
38	6.679535905845262E-4	0.0	0.0	23.255472209790863	0.0
39	6.679535905845262E-4	0.0	0.0	23.74207640053169	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAACG	35	1.2106284E-7	45.000004	1
TAGCCGT	25	3.8885588E-5	45.000004	44
TATTGCG	25	3.8885588E-5	45.000004	1
TACGATC	25	3.8885588E-5	45.000004	10
TACGACG	35	1.2106284E-7	45.000004	1
TATAGCG	60	0.0	45.000004	1
CGGTCAT	25	3.8885588E-5	45.000004	13
ATACGAT	25	3.8885588E-5	45.000004	9
TCGACAA	25	3.8885588E-5	45.000004	19
CGTTTAT	25	3.8885588E-5	45.000004	39
TGTAACG	30	2.1637534E-6	45.000004	1
TAGTACG	50	2.1827873E-11	45.000004	1
GTACGAG	30	2.1637534E-6	45.000004	1
CGGGTCC	40	6.8048394E-9	45.0	6
AACCCGT	20	7.0305774E-4	45.0	29
CGTTGAT	45	3.8380676E-10	45.0	25
ACGGGTA	110	0.0	45.0	5
CCCGTTC	20	7.0305774E-4	45.0	31
ACTAGTC	20	7.0305774E-4	45.0	12
TAACCGG	20	7.0305774E-4	45.0	2
>>END_MODULE