FastQCFastQC Report
Sat 18 Jun 2016
SRR3551206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688248
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC33540.4873243365763504No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC24850.36106171031372414No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT18810.27330264672036825Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.22390184933337984No Hit
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC12920.18772303007055596No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC9110.13236507770454836No Hit
ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC8980.13047622368681058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG207.031311E-445.0000049
TTCGTCA207.031311E-445.00000433
ACGTGCG207.031311E-445.0000041
GTCCGCA207.031311E-445.00000432
TCGTAAG302.1642263E-645.0000041
TATAGCG207.031311E-445.0000041
TAGTGCG351.210974E-745.0000041
CAATCGT207.031311E-445.00000442
CGATCAC207.031311E-445.00000410
TAAGACG302.1642263E-645.0000041
GTACGAG207.031311E-445.0000041
CTCGACG253.889168E-545.01
CGGTCTA253.889168E-545.031
ATCGCAT253.889168E-545.010
ATCGATA253.889168E-545.017
TCGATCA750.042.017
TTACGCG750.042.01
TACTTAT1300.041.5384614
CGTAAGG603.6379788E-1241.2500042
TCATGCG501.0804797E-940.51