##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	688248
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2801766223803	33.0	31.0	34.0	30.0	34.0
2	32.37007444990759	34.0	31.0	34.0	30.0	34.0
3	32.4005968778696	34.0	31.0	34.0	30.0	34.0
4	35.913422196650046	37.0	35.0	37.0	35.0	37.0
5	35.97323493856866	37.0	35.0	37.0	35.0	37.0
6	36.007173286373515	37.0	35.0	37.0	35.0	37.0
7	36.17436592623589	37.0	36.0	37.0	35.0	37.0
8	35.85312997640386	37.0	35.0	37.0	35.0	37.0
9	37.727740581883275	39.0	38.0	39.0	35.0	39.0
10	37.572822587206936	39.0	37.0	39.0	35.0	39.0
11	37.50582057630389	39.0	37.0	39.0	35.0	39.0
12	37.32501946972603	39.0	37.0	39.0	35.0	39.0
13	37.2441939533424	39.0	37.0	39.0	34.0	39.0
14	38.435569736490336	40.0	38.0	41.0	34.0	41.0
15	38.53057328172403	40.0	38.0	41.0	35.0	41.0
16	38.59502679266776	40.0	38.0	41.0	35.0	41.0
17	38.60149103230231	40.0	38.0	41.0	35.0	41.0
18	38.60242964745266	40.0	38.0	41.0	35.0	41.0
19	38.59423056805105	40.0	37.0	41.0	35.0	41.0
20	38.5234944380514	40.0	37.0	41.0	35.0	41.0
21	38.45660866431868	40.0	37.0	41.0	34.0	41.0
22	38.44721815392126	40.0	37.0	41.0	35.0	41.0
23	38.417891515848936	40.0	37.0	41.0	34.0	41.0
24	38.36761893968453	40.0	37.0	41.0	34.0	41.0
25	38.2910477037347	40.0	37.0	41.0	34.0	41.0
26	38.306694679824716	40.0	37.0	41.0	34.0	41.0
27	38.27776034220223	40.0	37.0	41.0	34.0	41.0
28	38.20038271088329	40.0	37.0	41.0	34.0	41.0
29	38.18016034917646	40.0	37.0	41.0	34.0	41.0
30	38.098313398658625	40.0	36.0	41.0	34.0	41.0
31	38.0488108937476	40.0	36.0	41.0	34.0	41.0
32	38.01104543710988	40.0	36.0	41.0	34.0	41.0
33	37.97901773779219	40.0	36.0	41.0	34.0	41.0
34	37.89237745696319	40.0	36.0	41.0	34.0	41.0
35	37.82007357812881	40.0	36.0	41.0	34.0	41.0
36	37.7590795759668	40.0	36.0	41.0	34.0	41.0
37	37.688920272924875	40.0	36.0	41.0	33.0	41.0
38	37.61110965814648	40.0	35.0	41.0	33.0	41.0
39	37.57809975473957	40.0	35.0	41.0	33.0	41.0
40	37.474895677139635	40.0	35.0	41.0	33.0	41.0
41	37.42782107612372	40.0	35.0	41.0	33.0	41.0
42	37.37438684892655	40.0	35.0	41.0	33.0	41.0
43	37.28906004812219	39.0	35.0	41.0	33.0	41.0
44	37.222852808871224	39.0	35.0	41.0	33.0	41.0
45	37.190174181399726	39.0	35.0	41.0	33.0	41.0
46	37.09312631493299	39.0	35.0	41.0	33.0	41.0
47	37.07682695772454	39.0	35.0	41.0	33.0	41.0
48	37.03298665597285	39.0	35.0	41.0	33.0	41.0
49	36.939671455638084	39.0	35.0	41.0	32.0	41.0
50	36.869009426834516	39.0	35.0	41.0	32.0	41.0
51	36.1199349653032	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	5.0
11	14.0
12	10.0
13	15.0
14	20.0
15	29.0
16	31.0
17	68.0
18	100.0
19	196.0
20	335.0
21	519.0
22	860.0
23	1350.0
24	2061.0
25	2799.0
26	3873.0
27	4668.0
28	5498.0
29	6287.0
30	7515.0
31	9672.0
32	12794.0
33	18496.0
34	38003.0
35	61279.0
36	41852.0
37	63683.0
38	120218.0
39	285480.0
40	507.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.318757192174914	21.23449686740826	24.09233299624554	12.354412944171287
2	32.72148992804919	24.56352942543967	24.063419000127862	18.65156164638328
3	31.874266252862338	23.89894340412177	26.056741174692842	18.170049168323047
4	30.329764852204438	24.84816519626646	26.084928688496007	18.73714126303309
5	25.328515302623472	29.6730248398833	24.84453278469389	20.15392707279934
6	24.845985749322917	34.06417454173496	24.96411177366298	16.125727935279144
7	77.7889656054213	6.3757540886424655	10.274639374179074	5.560640931757158
8	77.58831119015238	6.2144750148202395	9.748956771396356	6.448257023631017
9	71.4714753984029	8.437946786623426	12.355430019411608	7.7351477955620656
10	40.0088340249445	25.527862049726263	19.39315479303972	15.070149132289522
11	30.568341644291014	25.844898931780403	24.356481965803027	19.23027745812556
12	28.277452313700874	22.00878171821785	29.516249956411063	20.19751601167021
13	24.232544082946845	25.84896723274169	29.67462310097523	20.24386558333624
14	19.56184398646999	28.916030268159155	29.738262951726703	21.78386279364415
15	19.78618172519208	25.894154432704493	34.83962176424777	19.480042077855657
16	21.19105322500029	26.350966512071228	30.88683149097418	21.571148771954295
17	21.433843614511048	24.486958189489837	28.478833211284304	25.600364984714812
18	22.52385767920866	26.118346874963677	29.73230579674768	21.62548964907998
19	23.563889760667667	27.616789296881354	27.03807348513908	21.7812474573119
20	24.750525973195707	27.425143262312424	27.654275784310308	20.17005498018156
21	23.08426613662517	28.135061780056027	28.745597517174044	20.03507456614476
22	21.735043182108775	25.2398844602527	27.652386930292565	25.37268542734596
23	21.00216782322651	26.872435517429764	28.26771745068638	23.857679208657345
24	22.46995269147168	25.570433913356815	29.03488277481373	22.924730620357778
25	20.83914519184945	26.773198033267075	28.039602003928817	24.348054770954654
26	19.507938998733014	28.13680533761086	28.481593844079462	23.873661819576665
27	21.834425962734365	26.49757064314026	28.6685903918355	22.999413002289874
28	18.435941695435364	26.33556508700352	31.487196475688993	23.741296741872116
29	19.80013018563076	25.111006497657822	30.251014169311063	24.837849147400355
30	21.655130127512177	25.210389278283408	29.634521277214027	23.499959316990388
31	22.080558170891887	25.967674442933365	28.353297067336193	23.598470318838558
32	22.771733444921015	26.13403889295719	28.003423184665994	23.0908044774558
33	22.3561855610187	24.2852866989806	28.422748776603786	24.935778963396913
34	20.088834257418835	23.59643616835792	31.465983192105174	24.848746382118073
35	21.408707326428843	23.17667468703142	30.462420522834794	24.952197463704945
36	23.145726540433103	24.85455824063419	28.926636909951064	23.073078308981646
37	21.98161127965501	24.497274238355942	32.6670037544606	20.85411072752845
38	22.102352640327325	26.226011553974732	28.1439248642931	23.52771094140484
39	23.45956690030338	23.00478897141728	29.392312073554884	24.14333205472446
40	22.979071497483467	22.735699922121096	30.171827597028976	24.11340098336646
41	21.587131382873615	23.7256047238786	28.46473945438272	26.222524438865058
42	24.108025014239054	23.287535888226337	28.330776115586243	24.27366298194837
43	23.496908091269425	22.17311201776104	30.12489683951134	24.205083051458196
44	20.645900896188582	23.640170403691695	30.16441747742093	25.549511222698797
45	20.888400692773534	23.05767688391394	29.464960305006333	26.588962118306192
46	22.761562692517813	22.983139798444746	29.79710801920238	24.458189489835057
47	20.903802117841245	22.780596529158096	32.22966140112285	24.085939951877812
48	21.807546117097328	21.78676872290221	30.984616010507843	25.421069149492624
49	22.311724843370413	21.1165161395311	32.397769408701514	24.173989608396973
50	21.299734979251664	21.064790598737666	31.369070451348936	26.266403970661738
51	20.78422312887215	21.866391184573004	28.47316664923109	28.876219037323757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	281.0
1	760.0
2	1239.0
3	2136.0
4	3033.0
5	2137.5
6	1242.0
7	1239.5
8	1237.0
9	1235.0
10	1233.0
11	1248.0
12	1263.0
13	1222.0
14	1181.0
15	1236.5
16	1292.0
17	1231.0
18	1170.0
19	1153.5
20	1137.0
21	1822.0
22	2507.0
23	2510.5
24	2514.0
25	3077.5
26	4492.5
27	5344.0
28	6616.0
29	7888.0
30	9352.5
31	10817.0
32	11955.5
33	13094.0
34	14432.5
35	15771.0
36	17253.0
37	18735.0
38	20889.5
39	23044.0
40	26464.0
41	29884.0
42	34238.0
43	38592.0
44	44737.5
45	50883.0
46	58315.5
47	65748.0
48	73203.0
49	80658.0
50	76379.0
51	72100.0
52	61196.0
53	50292.0
54	44451.0
55	38610.0
56	35046.0
57	31482.0
58	28729.0
59	25976.0
60	24025.0
61	22074.0
62	20853.5
63	19633.0
64	17036.0
65	14439.0
66	12511.5
67	10584.0
68	8683.5
69	6783.0
70	5527.0
71	4271.0
72	3962.0
73	3653.0
74	3062.5
75	1900.5
76	1329.0
77	989.5
78	650.0
79	467.5
80	285.0
81	179.5
82	74.0
83	71.0
84	68.0
85	49.0
86	30.0
87	19.5
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	688248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.697037357799168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.7811023244504	18.3625165826646
2	12.679186284448635	6.769934198048401
3	5.13513388038866	4.112785831261088
4	2.6917265135943302	2.874444931616255
5	1.5585013634662739	2.080368456132003
6	0.9903650441116175	1.5863887548303768
7	0.7008903145731719	1.3098186438315709
8	0.536555459942287	1.1459552926888283
9	0.3986208386086436	0.9577795877938974
>10	5.387847964264303	36.931769740612644
>50	1.0409334893558564	17.280428442611573
>100	0.09362893819602354	4.527538418250308
>500	0.002753792299883045	0.5085981874749225
>1k	0.002753792299883045	1.5516729321835379
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	3354	0.4873243365763504	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	2485	0.36106171031372414	No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	1881	0.27330264672036825	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1541	0.22390184933337984	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	1292	0.18772303007055596	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC	911	0.13236507770454836	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	898	0.13047622368681058	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9059292580581417E-4	0.0	0.0	0.28202043449454267	0.0
2	4.3588938870872126E-4	0.0	0.0	1.4821692180725552	0.0
3	4.3588938870872126E-4	0.0	0.0	2.024996803477816	0.0
4	4.3588938870872126E-4	0.0	0.0	2.7469749276423614	0.0
5	4.3588938870872126E-4	0.0	0.0	4.597470679173786	0.0
6	4.3588938870872126E-4	0.0	0.0	5.642588136834397	0.0
7	4.3588938870872126E-4	0.0	0.0	6.731003940440074	0.0
8	4.3588938870872126E-4	0.0	0.0	8.428502516534737	0.0
9	4.3588938870872126E-4	0.0	0.0	9.099481582220362	0.0
10	4.3588938870872126E-4	0.0	0.0	10.566830561076822	0.0
11	4.3588938870872126E-4	0.0	0.0	12.442753193616255	0.0
12	4.3588938870872126E-4	0.0	0.0	13.998151828991874	0.0
13	4.3588938870872126E-4	0.0	0.0	14.66055840336623	0.0
14	4.3588938870872126E-4	0.0	0.0	14.924997965849519	0.0
15	4.3588938870872126E-4	0.0	0.0	15.405638665132336	0.0
16	4.3588938870872126E-4	0.0	0.0	16.319117527402913	0.0
17	4.3588938870872126E-4	0.0	0.0	17.44865223001011	0.0
18	4.3588938870872126E-4	0.0	0.0	18.794823958805548	0.0
19	4.3588938870872126E-4	0.0	0.0	19.488033383315315	0.0
20	5.811858516116283E-4	0.0	0.0	20.224250560844347	0.0
21	5.811858516116283E-4	0.0	0.0	21.009868535760365	0.0
22	5.811858516116283E-4	0.0	0.0	21.875544861735886	0.0
23	5.811858516116283E-4	0.0	0.0	22.662615801280932	0.0
24	5.811858516116283E-4	0.0	0.0	23.321389964082716	0.0
25	5.811858516116283E-4	0.0	0.0	23.921900245260428	0.0
26	5.811858516116283E-4	0.0	0.0	24.45659122874313	0.0
27	5.811858516116283E-4	0.0	0.0	24.976897862398438	0.0
28	5.811858516116283E-4	0.0	0.0	25.537451616277853	0.0
29	5.811858516116283E-4	0.0	0.0	26.111953830595947	0.0
30	5.811858516116283E-4	0.0	0.0	26.743412258371983	0.0
31	5.811858516116283E-4	0.0	0.0	27.34058072090293	0.0
32	5.811858516116283E-4	0.0	0.0	27.902442142948473	0.0
33	7.264823145145355E-4	0.0	0.0	28.458927595866605	0.0
34	7.264823145145355E-4	0.0	0.0	29.032558031407284	0.0
35	7.264823145145355E-4	0.0	0.0	29.60604317048506	0.0
36	7.264823145145355E-4	0.0	0.0	30.162092734014482	0.0
37	7.264823145145355E-4	0.0	0.0	30.68123699596657	0.0
38	7.264823145145355E-4	0.0	0.0	31.20444955887994	0.0
39	7.264823145145355E-4	0.0	0.0	31.76035382594646	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCG	20	7.031311E-4	45.000004	9
TTCGTCA	20	7.031311E-4	45.000004	33
ACGTGCG	20	7.031311E-4	45.000004	1
GTCCGCA	20	7.031311E-4	45.000004	32
TCGTAAG	30	2.1642263E-6	45.000004	1
TATAGCG	20	7.031311E-4	45.000004	1
TAGTGCG	35	1.210974E-7	45.000004	1
CAATCGT	20	7.031311E-4	45.000004	42
CGATCAC	20	7.031311E-4	45.000004	10
TAAGACG	30	2.1642263E-6	45.000004	1
GTACGAG	20	7.031311E-4	45.000004	1
CTCGACG	25	3.889168E-5	45.0	1
CGGTCTA	25	3.889168E-5	45.0	31
ATCGCAT	25	3.889168E-5	45.0	10
ATCGATA	25	3.889168E-5	45.0	17
TCGATCA	75	0.0	42.0	17
TTACGCG	75	0.0	42.0	1
TACTTAT	130	0.0	41.53846	14
CGTAAGG	60	3.6379788E-12	41.250004	2
TCATGCG	50	1.0804797E-9	40.5	1
>>END_MODULE