##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	714146
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32096798133715	33.0	31.0	34.0	30.0	34.0
2	32.42191652687266	34.0	31.0	34.0	30.0	34.0
3	32.445983034281504	34.0	31.0	34.0	30.0	34.0
4	35.942660184332055	37.0	35.0	37.0	35.0	37.0
5	35.98622130488724	37.0	35.0	37.0	35.0	37.0
6	36.01470007533474	37.0	35.0	37.0	35.0	37.0
7	36.13656311174466	37.0	36.0	37.0	35.0	37.0
8	35.80280362838971	37.0	35.0	37.0	35.0	37.0
9	37.66032996054028	39.0	38.0	39.0	35.0	39.0
10	37.570599009166195	39.0	37.0	39.0	35.0	39.0
11	37.48549036191479	39.0	37.0	39.0	35.0	39.0
12	37.33632898594965	39.0	37.0	39.0	35.0	39.0
13	37.25542536120065	39.0	37.0	39.0	34.0	39.0
14	38.47794288562843	40.0	38.0	41.0	34.0	41.0
15	38.55923718679374	40.0	38.0	41.0	35.0	41.0
16	38.606429217554954	40.0	38.0	41.0	35.0	41.0
17	38.6313246871088	40.0	38.0	41.0	35.0	41.0
18	38.621748213950646	40.0	38.0	41.0	35.0	41.0
19	38.6177714360929	40.0	38.0	41.0	35.0	41.0
20	38.551706513794095	40.0	38.0	41.0	35.0	41.0
21	38.46039325292027	40.0	37.0	41.0	34.0	41.0
22	38.45134468302	40.0	37.0	41.0	34.0	41.0
23	38.43659839864677	40.0	37.0	41.0	34.0	41.0
24	38.37227681734547	40.0	37.0	41.0	34.0	41.0
25	38.28034043458901	40.0	37.0	41.0	34.0	41.0
26	38.27231126408326	40.0	37.0	41.0	34.0	41.0
27	38.23028764426322	40.0	37.0	41.0	34.0	41.0
28	38.16936592797551	40.0	37.0	41.0	34.0	41.0
29	38.14490874415036	40.0	37.0	41.0	34.0	41.0
30	38.065107134955596	40.0	36.0	41.0	34.0	41.0
31	38.01455024602812	40.0	36.0	41.0	34.0	41.0
32	37.959917159796454	40.0	36.0	41.0	34.0	41.0
33	37.939826029971464	40.0	36.0	41.0	34.0	41.0
34	37.86550789334394	40.0	36.0	41.0	34.0	41.0
35	37.799070498189444	40.0	36.0	41.0	33.0	41.0
36	37.750784853517345	40.0	36.0	41.0	33.0	41.0
37	37.67698341795655	40.0	36.0	41.0	33.0	41.0
38	37.57655717458335	40.0	35.0	41.0	33.0	41.0
39	37.5319654524425	40.0	35.0	41.0	33.0	41.0
40	37.44764655966707	40.0	35.0	41.0	33.0	41.0
41	37.37785690881137	40.0	35.0	41.0	33.0	41.0
42	37.356004234428255	40.0	35.0	41.0	33.0	41.0
43	37.273838962901145	40.0	35.0	41.0	33.0	41.0
44	37.19311037238884	39.0	35.0	41.0	33.0	41.0
45	37.12120770822773	39.0	35.0	41.0	33.0	41.0
46	36.999617725227054	39.0	35.0	41.0	32.0	41.0
47	36.96440643789925	39.0	35.0	41.0	32.0	41.0
48	36.9057181584718	39.0	35.0	41.0	32.0	41.0
49	36.80758556373627	39.0	35.0	41.0	32.0	41.0
50	36.75822730926169	39.0	35.0	41.0	32.0	41.0
51	35.988611572423565	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	9.0
10	10.0
11	11.0
12	16.0
13	15.0
14	23.0
15	43.0
16	62.0
17	81.0
18	173.0
19	217.0
20	374.0
21	632.0
22	902.0
23	1297.0
24	2012.0
25	2874.0
26	4041.0
27	4959.0
28	5609.0
29	6593.0
30	8054.0
31	10369.0
32	13714.0
33	19246.0
34	38970.0
35	60758.0
36	46142.0
37	71406.0
38	125421.0
39	289569.0
40	537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.850341526802644	21.586622343330355	22.426646652085147	13.136389477781854
2	32.18277495078038	25.049219627359108	23.538744178361288	19.22926124349923
3	32.58927446208478	23.47503171620369	25.805927639446274	18.12976618226525
4	29.911110613235948	25.67052115393771	24.30077323124403	20.11759500158231
5	25.622071677220067	30.21805065070728	23.42420177386697	20.735675898205688
6	27.018564831281	33.38084929412193	23.656927294978896	15.943658579618173
7	77.14220901608354	7.371181803160698	9.690175398307908	5.796433782447847
8	77.21138254642608	6.49755092096014	9.489096067190744	6.801970465423037
9	70.91449087441504	9.005021382182354	12.309387716237296	7.771100027165313
10	42.39217190882537	24.029820232837544	18.552088788567044	15.025919069770048
11	31.003604305002057	25.239796904274474	23.191616280144398	20.564982510579068
12	29.653740271597123	22.559532644585282	27.186457671120472	20.600269412697124
13	25.51873146387433	25.165862442693793	28.389993082646964	20.92541301078491
14	20.42607534033657	28.497674145062774	28.248145337227964	22.82810517737269
15	20.17808683378469	26.273759147289212	32.78741321802545	20.760740800900656
16	22.268695756890047	26.467977136327864	30.68868270633736	20.57464440044473
17	22.171236693897324	25.86543927992315	26.74859202460001	25.21473200157951
18	23.995373495055635	25.811248680241857	28.178271669938642	22.01510615476387
19	26.02675083246283	25.701607234375047	26.187362248055717	22.084279685106406
20	26.3791997714753	26.419107577442148	26.553953953393282	20.64773869768927
21	25.2469383011317	26.616406169046662	27.92678247865282	20.20987305116881
22	23.85058517446012	25.23559608259375	26.60408375878322	24.309734984162905
23	23.553447054243808	25.59938724014417	27.281536268494115	23.5656294371179
24	23.482593195228986	24.733737919136985	28.245764871608888	23.537904014025145
25	23.44156517014728	26.25961638096412	26.349513964931543	23.94930448395706
26	21.2163899258695	27.128346304537164	26.454674534338917	25.200589235254416
27	22.681356473326183	26.764835201765464	27.433746040725566	23.120062284182787
28	21.252236937544982	27.435846451565926	27.352530154898297	23.959386455990792
29	21.555396235503665	27.108742470027135	26.658974495411304	24.676886799057897
30	22.17795800858648	26.509985353135075	26.46713697199172	24.844919666286724
31	22.609102340417785	28.5970935915065	24.814533722796178	23.979270345279538
32	22.90932106319996	28.758965253603602	24.587269269869186	23.744444413327248
33	22.18635965194792	28.460566886883075	23.810257286325207	25.542816174843804
34	20.51303234912749	27.92230160219339	27.180576520767463	24.38408952791166
35	22.455352266903404	25.86095840346372	26.000005601095573	25.683683728537304
36	22.032329523654827	28.33468226385081	23.990052454260052	25.64293575823431
37	22.514863907380285	28.47470965320817	26.14843463381437	22.861991805597174
38	21.897343120314332	29.566083125859414	24.750681233249225	23.785892520577026
39	22.64971028333142	26.2022051513276	26.569777048390662	24.578307516950314
40	23.237265209074895	24.23019942700792	28.800273333464027	23.732262030453157
41	20.59046749544211	24.594270639337054	27.502219434121315	27.31304243109952
42	22.233828936940064	25.05314039426112	26.18260131681757	26.53042935198125
43	22.903299885457596	25.063782475852275	26.28930218750788	25.74361545118225
44	21.72889017091743	26.173499536509343	26.28286092759744	25.814749364975793
45	22.491339305968246	24.706432578212297	26.220968821501483	26.581259294317967
46	21.853654574834838	26.844370758920444	25.952676343492787	25.349298322751928
47	21.353196685271637	26.0147084769781	28.318439086685355	24.313655751064907
48	21.23487354126467	24.7445200281175	27.450829382227166	26.569777048390662
49	21.391704217344913	24.698170962240216	28.93792025720231	24.972204563212564
50	20.764661567802662	24.41475552618092	28.38411193229396	26.436470973722457
51	20.441898435333954	24.92417516866299	26.04901518737065	28.58491120863241
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	396.0
1	972.5
2	1549.0
3	2139.5
4	2730.0
5	1988.5
6	1247.0
7	1224.0
8	1201.0
9	1198.0
10	1195.0
11	1180.5
12	1166.0
13	1341.5
14	1517.0
15	1342.5
16	1168.0
17	1208.0
18	1248.0
19	1320.5
20	1393.0
21	1790.0
22	2187.0
23	2360.5
24	2534.0
25	3090.0
26	4678.0
27	5710.0
28	6674.5
29	7639.0
30	9157.0
31	10675.0
32	11815.0
33	12955.0
34	14048.0
35	15141.0
36	17272.0
37	19403.0
38	20767.5
39	22132.0
40	25899.0
41	29666.0
42	33522.0
43	37378.0
44	41812.0
45	46246.0
46	52208.5
47	58171.0
48	64719.0
49	71267.0
50	69768.0
51	68269.0
52	60384.5
53	52500.0
54	46736.5
55	40973.0
56	38042.5
57	35112.0
58	34242.0
59	33372.0
60	32516.0
61	31660.0
62	29682.0
63	27704.0
64	24564.5
65	21425.0
66	18360.5
67	15296.0
68	13442.0
69	11588.0
70	9207.5
71	6827.0
72	5489.5
73	4152.0
74	3499.5
75	2020.5
76	1194.0
77	1180.0
78	1166.0
79	698.5
80	231.0
81	229.0
82	227.0
83	128.5
84	30.0
85	17.0
86	4.0
87	4.5
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	714146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.34884724561935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3927073023754	18.811155391615028
2	10.996524774443571	5.7949150302896495
3	4.12852202982366	3.2634538894199387
4	2.035195011093932	2.1450016984944233
5	1.2061216925744338	1.588995811863581
6	0.8281204407812554	1.3092011397072156
7	0.6234262314799958	1.1498593779524977
8	0.5152732877838192	1.086148571965113
9	0.42102290237643814	0.9984121327461669
>10	7.094648761026955	47.49502691505588
>50	0.6724956323888703	10.645985287170575
>100	0.07734774046643825	3.574750354067538
>500	0.006445645038869854	1.146529915998936
>1k	0.0021485483462899513	0.9905644836534444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	2308	0.32318321463678296	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	2049	0.28691612079322715	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT	1325	0.18553629089849974	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1317	0.1844160717836409	No Hit
TCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	920	0.12882519820876964	Illumina Single End Adapter 1 (95% over 21bp)
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	899	0.1258846230322651	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	826	0.11566262360917796	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.400273893573583E-4	0.0	0.0	0.1914174412515088	0.0
2	1.400273893573583E-4	0.0	0.0	1.0572067896480553	0.0
3	1.400273893573583E-4	0.0	0.0	1.4834501628518537	0.0
4	1.400273893573583E-4	0.0	0.0	1.9983308735188603	0.0
5	1.400273893573583E-4	0.0	0.0	3.457836352790606	0.0
6	1.400273893573583E-4	0.0	0.0	4.322225427293579	0.0
7	1.400273893573583E-4	0.0	0.0	5.107078944641572	0.0
8	1.400273893573583E-4	0.0	0.0	6.273787152767081	0.0
9	1.400273893573583E-4	0.0	0.0	6.758421947332899	0.0
10	1.400273893573583E-4	0.0	0.0	7.751916274823356	0.0
11	1.400273893573583E-4	0.0	0.0	9.112002307651377	0.0
12	1.400273893573583E-4	0.0	0.0	10.15506633097434	0.0
13	1.400273893573583E-4	0.0	0.0	10.607214771209248	0.0
14	2.800547787147166E-4	0.0	0.0	10.790370596488673	0.0
15	2.800547787147166E-4	0.0	0.0	11.15416175403909	0.0
16	2.800547787147166E-4	0.0	0.0	11.848417550472872	0.0
17	2.800547787147166E-4	0.0	0.0	12.61758800021284	0.0
18	2.800547787147166E-4	0.0	0.0	13.4721751574608	0.0
19	2.800547787147166E-4	0.0	0.0	13.966191787113559	0.0
20	2.800547787147166E-4	0.0	0.0	14.488774004195221	0.0
21	4.200821680720749E-4	0.0	0.0	15.102233996969808	0.0
22	4.200821680720749E-4	0.0	0.0	15.755881850489956	0.0
23	4.200821680720749E-4	0.0	0.0	16.358699761673382	0.0
24	4.200821680720749E-4	0.0	0.0	16.873300417561676	0.0
25	5.601095574294332E-4	0.0	0.0	17.342252144519467	0.0
26	5.601095574294332E-4	0.0	0.0	17.7815180649335	0.0
27	7.001369467867915E-4	0.0	0.0	18.201600233005575	0.0
28	7.001369467867915E-4	0.0	0.0	18.62504305842223	0.0
29	7.001369467867915E-4	0.0	0.0	19.038123857026434	0.0
30	7.001369467867915E-4	0.0	0.0	19.5275195828304	0.0
31	7.001369467867915E-4	0.0	0.0	19.9793879682866	0.0
32	8.401643361441497E-4	0.0	0.0	20.460662105507836	0.0
33	8.401643361441497E-4	0.0	0.0	20.887885670437136	0.0
34	8.401643361441497E-4	0.0	0.0	21.32449107045338	0.0
35	8.401643361441497E-4	0.0	0.0	21.783920934934873	0.0
36	8.401643361441497E-4	0.0	0.0	22.237609676452713	0.0
37	8.401643361441497E-4	0.0	0.0	22.688497870183408	0.0
38	8.401643361441497E-4	0.0	0.0	23.125943434535795	0.0
39	8.401643361441497E-4	0.0	0.0	23.57403108047934	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTACG	30	2.1643427E-6	45.000004	1
GTTAACG	30	2.1643427E-6	45.000004	1
TCGCACG	25	3.8893162E-5	45.0	1
GATCGTA	20	7.0314907E-4	45.0	10
TAAGTCG	20	7.0314907E-4	45.0	43
ATCGTAA	20	7.0314907E-4	45.0	11
CGAATCG	20	7.0314907E-4	45.0	24
CGAATAT	105	0.0	45.0	14
CTCGAAT	20	7.0314907E-4	45.0	11
ATAACGG	25	3.8893162E-5	45.0	2
ATAACGC	40	6.8084773E-9	45.0	11
TTCGCAA	40	6.8084773E-9	45.0	14
ACGAGAT	20	7.0314907E-4	45.0	43
CGTCAAT	25	3.8893162E-5	45.0	36
CAATACG	20	7.0314907E-4	45.0	20
ATACCCG	20	7.0314907E-4	45.0	22
TCGAAAC	20	7.0314907E-4	45.0	21
CTAGCGT	25	3.8893162E-5	45.0	14
TAACGGG	170	0.0	43.676468	3
ATAGCGG	65	0.0	41.538464	2
>>END_MODULE