FastQCFastQC Report
Sat 18 Jun 2016
SRR3551200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999358
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81050.8110206752735256No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC20330.20343060244677083TruSeq Adapter, Index 14 (95% over 22bp)
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC17710.17721377124113682No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG13910.13918935956884318No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG12400.12407965914116863No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12210.12217843855755395No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC11170.11177175746829465TruSeq Adapter, Index 14 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG253.8904393E-545.01
CATACCG207.032846E-445.029
TATAGCG551.8189894E-1245.01
ATCGCTT207.032846E-445.044
TACCGTG207.032846E-445.017
CGTTTTT32850.040.01
CGTAAGG850.039.7058832
TCGGCGT2450.038.571434
GCGATCG356.2474683E-638.571439
GATCGCG301.13981514E-437.5000041
TAACGCC301.13981514E-437.50000412
CGTTAGG1450.037.241382
CGCTAGT3650.036.3698654
ATAGCGG1800.036.252
TACAGCG751.8189894E-1236.0000041
TTTGTCG250.002106977436.01
ATTCGCA250.002106977436.030
TCGATTA250.002106977436.041
GTTCGGA250.002106977436.012
CCGGTAT250.002106977436.042
Produced by FastQC (version 0.11.5)