##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	999358
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.458789542886535	34.0	31.0	34.0	31.0	34.0
2	32.57553949635666	34.0	31.0	34.0	31.0	34.0
3	32.568090714238544	34.0	31.0	34.0	31.0	34.0
4	36.042560323727834	37.0	35.0	37.0	35.0	37.0
5	36.07117569479606	37.0	35.0	37.0	35.0	37.0
6	36.092618461052	37.0	36.0	37.0	35.0	37.0
7	36.185785274146	37.0	37.0	37.0	35.0	37.0
8	35.86204343188327	37.0	36.0	37.0	35.0	37.0
9	37.750688942300954	39.0	38.0	39.0	35.0	39.0
10	37.658121514011995	39.0	37.0	39.0	35.0	39.0
11	37.584295117465416	39.0	37.0	39.0	35.0	39.0
12	37.47131258267808	39.0	37.0	39.0	35.0	39.0
13	37.45079741193847	39.0	37.0	39.0	35.0	39.0
14	38.74121285865526	40.0	38.0	41.0	35.0	41.0
15	38.812380548312014	40.0	38.0	41.0	35.0	41.0
16	38.851716802187006	40.0	38.0	41.0	35.0	41.0
17	38.84660251881708	40.0	38.0	41.0	35.0	41.0
18	38.8057612987538	40.0	38.0	41.0	35.0	41.0
19	38.751602528823504	40.0	38.0	41.0	35.0	41.0
20	38.64153786731082	40.0	38.0	41.0	35.0	41.0
21	38.5878283858237	40.0	38.0	41.0	35.0	41.0
22	38.55574078558435	40.0	37.0	41.0	35.0	41.0
23	38.52831617898691	40.0	37.0	41.0	35.0	41.0
24	38.476105659833614	40.0	37.0	41.0	35.0	41.0
25	38.39135825199778	40.0	37.0	41.0	35.0	41.0
26	38.3601702292872	40.0	37.0	41.0	35.0	41.0
27	38.309202508010145	40.0	37.0	41.0	34.0	41.0
28	38.227038758883204	40.0	37.0	41.0	34.0	41.0
29	38.20387889024754	40.0	37.0	41.0	34.0	41.0
30	38.11254425341069	40.0	37.0	41.0	34.0	41.0
31	38.043300799113034	40.0	36.0	41.0	34.0	41.0
32	37.92774161011369	40.0	36.0	41.0	34.0	41.0
33	37.82629848362649	40.0	36.0	41.0	34.0	41.0
34	37.709456471054416	40.0	36.0	41.0	33.0	41.0
35	37.5824989643351	40.0	36.0	41.0	33.0	41.0
36	37.51487354881834	40.0	36.0	41.0	33.0	41.0
37	37.45533532527883	40.0	36.0	41.0	33.0	41.0
38	37.356489866494286	40.0	35.0	41.0	33.0	41.0
39	37.26919482307642	40.0	35.0	41.0	33.0	41.0
40	37.13962463901825	40.0	35.0	41.0	33.0	41.0
41	37.048761304757655	40.0	35.0	41.0	32.0	41.0
42	36.988624697055506	40.0	35.0	41.0	32.0	41.0
43	36.925745328500895	39.0	35.0	41.0	32.0	41.0
44	36.8539302232033	39.0	35.0	41.0	32.0	41.0
45	36.785179084972555	39.0	35.0	41.0	32.0	41.0
46	36.64493805022825	39.0	35.0	41.0	31.0	41.0
47	36.57752577154533	39.0	35.0	41.0	31.0	41.0
48	36.49871317385762	39.0	35.0	41.0	31.0	41.0
49	36.39425511178177	39.0	35.0	41.0	31.0	41.0
50	36.32941748602603	39.0	35.0	41.0	31.0	41.0
51	35.49099621957296	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	17.0
10	19.0
11	13.0
12	24.0
13	34.0
14	42.0
15	74.0
16	91.0
17	156.0
18	225.0
19	361.0
20	604.0
21	991.0
22	1555.0
23	2313.0
24	3418.0
25	5266.0
26	7552.0
27	9469.0
28	10136.0
29	10628.0
30	11500.0
31	14000.0
32	17755.0
33	24977.0
34	50227.0
35	76170.0
36	65482.0
37	96002.0
38	171965.0
39	417286.0
40	1000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.501401900019815	21.51681379445604	22.88699344979477	15.094790855729379
2	34.14602174596091	25.296340250440785	22.590202910268392	17.967435093329918
3	31.536846655552864	24.43538751878706	26.589170247298767	17.43859557836131
4	29.340736752995422	24.058545586266384	27.12731573670296	19.47340192403523
5	26.377834569793805	28.283558044264417	24.830241014731456	20.508366371210318
6	26.31569467598198	33.61948370854088	24.211643875367987	15.853177740109151
7	76.56405412274681	7.0465238683234634	11.269234848772912	5.120187160156821
8	77.69067741490036	6.596434911212999	10.517952525521384	5.194935148365251
9	71.69853045655312	8.379979947126055	12.534146922324133	7.387342673996707
10	41.397977501556	23.77086089269311	19.713956359983108	15.117205245767785
11	29.79853065668159	25.422221065924322	25.15174742184482	19.62750085554926
12	28.027193458200166	22.83726152189706	28.53051659165184	20.60502842825094
13	24.825938252358014	23.13495263959462	30.640971503705376	21.398137604341986
14	20.737413419415265	26.941996761921157	30.122438605584783	22.198151213078795
15	20.596322839262807	26.60017731383548	32.317948122694766	20.485551724206942
16	22.920915227576106	25.831483812607694	31.019214335603458	20.228386624212742
17	22.63913432423616	25.914537132839282	28.52251145235241	22.923817090572147
18	23.23751848686857	26.22773820792949	28.814699036781615	21.720044268420327
19	24.481917390965	26.511120139129318	27.77953446112404	21.227428008781636
20	25.9866834507754	26.044220389490054	28.382821771577355	19.586274388157197
21	25.42062003806444	26.009197905055043	28.747055609701427	19.82312644717909
22	23.538911981492117	24.4847191897198	29.072664650705754	22.90370417808233
23	23.788071942186882	25.233599971181498	28.986109082030666	21.992219004600955
24	23.467466113244704	25.136537657175907	29.19704450257065	22.19895172700874
25	23.46156232301137	26.843033227331947	27.908617332327356	21.786787117329325
26	22.175536694557906	27.811655082563007	27.60422191046652	22.408586312412567
27	23.098329127299728	26.965311730130743	28.92326873853014	21.013090404039396
28	21.167689656759638	26.88666123651384	29.722481833337	22.223167273389517
29	22.412688946303525	26.404151465240684	28.99801672673857	22.18514286171722
30	22.345946097394528	26.416459366913557	29.206050284282508	22.031544251409404
31	23.07411358091895	26.688734167335433	27.778133561746642	22.45901868999898
32	23.54421538627799	27.049065500051032	27.86358842376806	21.543130689902917
33	23.09182495161894	26.432769838236148	27.33344807366329	23.141957136481622
34	22.86928207909478	26.08994974773805	27.888104162872562	23.15266401029461
35	23.067109084031948	25.550003101991482	28.32848688858247	23.054400925394102
36	22.576494109218118	27.330246017943523	27.40159182194969	22.69166805088867
37	23.23201495360021	26.698840655700963	28.501497961691406	21.56764642900742
38	22.72108693781408	26.042319168906435	27.660357949803778	23.576235943475712
39	24.1418990992217	24.498127798046347	27.72509951388792	23.634873588844037
40	23.21350306897028	24.723272340842822	29.54056504275745	22.52265954742945
41	21.085436850457995	25.85860122198451	29.31742178478583	23.738540142771658
42	21.3951356771047	26.346514462284787	29.047848718877518	23.21050114173299
43	22.714382633650803	25.130333674218846	28.7509581151099	23.404325577020447
44	22.934223771661408	25.64366323179481	27.40099143650224	24.021121560041546
45	23.0368896831766	24.92169973122745	27.62963822774221	24.411772357853742
46	23.059103944732517	26.166699020771333	27.67246572299416	23.101731311501982
47	22.79253280606149	25.965970152838125	29.191741097784778	22.049755943315606
48	21.86243568370894	24.915395684029146	29.765409392830193	23.456759239431715
49	21.630987093714165	25.066292559823406	29.40337696801347	23.899343378448965
50	20.887910038244552	24.70786244769142	30.333273961883528	24.070953552180498
51	21.470784243484317	24.633614780689204	28.179191040648092	25.716409935178387
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	751.0
1	1410.5
2	2070.0
3	7400.5
4	12731.0
5	8275.0
6	3819.0
7	3676.5
8	3534.0
9	3417.5
10	3301.0
11	3357.5
12	3414.0
13	3348.5
14	3283.0
15	3206.0
16	3129.0
17	3064.0
18	2999.0
19	3018.5
20	3038.0
21	4038.5
22	5039.0
23	4923.0
24	4807.0
25	5257.5
26	7432.5
27	9157.0
28	11253.5
29	13350.0
30	15280.0
31	17210.0
32	19033.5
33	20857.0
34	22447.5
35	24038.0
36	26009.0
37	27980.0
38	30902.0
39	33824.0
40	37552.5
41	41281.0
42	44868.5
43	48456.0
44	54018.0
45	59580.0
46	69772.5
47	79965.0
48	83837.5
49	87710.0
50	84658.0
51	81606.0
52	75827.0
53	70048.0
54	63127.5
55	56207.0
56	53091.0
57	49975.0
58	49262.5
59	48550.0
60	46641.5
61	44733.0
62	41296.0
63	37859.0
64	33823.0
65	29787.0
66	25603.5
67	21420.0
68	17871.0
69	14322.0
70	12180.5
71	10039.0
72	8008.0
73	5977.0
74	4944.5
75	2958.5
76	2005.0
77	1563.0
78	1121.0
79	819.0
80	517.0
81	324.5
82	132.0
83	94.0
84	56.0
85	36.5
86	17.0
87	15.0
88	13.0
89	18.5
90	24.0
91	14.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	999358.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.777204506172684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.88278426050024	18.787144348621787
2	8.75541593183841	4.513802940232024
3	3.1764318287109323	2.4563859854579335
4	1.7434649741431532	1.7976661275134853
5	1.1711298493531543	1.5094226815029737
6	0.8709912678785358	1.3471032021117393
7	0.728504695718989	1.3145170167678841
8	0.6164295278005976	1.2711864001407571
9	0.5579948346037347	1.2945192268471624
>10	9.07044104068813	54.11610759647621
>50	0.35095459040615834	5.444996330197168
>100	0.06484602983886299	3.024693916170601
>500	0.007860124828953078	1.4126111788553042
>1k	0.002358037448685923	0.8887577360942043
>5k	3.930062414476538E-4	0.8210853130107746
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8105	0.8110206752735256	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2033	0.20343060244677083	TruSeq Adapter, Index 14 (95% over 22bp)
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	1771	0.17721377124113682	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1391	0.13918935956884318	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG	1240	0.12407965914116863	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1221	0.12217843855755395	No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1117	0.11177175746829465	TruSeq Adapter, Index 14 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.001927237286338E-4	0.0	0.0	0.062239958053070074	0.0
2	3.001927237286338E-4	0.0	0.0	0.34472131108171444	0.0
3	3.001927237286338E-4	0.0	0.0	0.586976839130722	0.0
4	3.001927237286338E-4	0.0	0.0	0.9231926897067918	0.0
5	3.001927237286338E-4	0.0	0.0	1.921333496104499	0.0
6	3.001927237286338E-4	0.0	0.0	2.7449622657746273	0.0
7	3.001927237286338E-4	0.0	0.0	3.3449474562669232	0.0
8	3.001927237286338E-4	0.0	0.0	4.090225925043878	0.0
9	3.001927237286338E-4	0.0	0.0	4.479475823478674	0.0
10	3.001927237286338E-4	0.0	0.0	5.283992323071412	0.0
11	3.001927237286338E-4	0.0	0.0	6.237904734839767	0.0
12	4.002569649715117E-4	0.0	0.0	7.045122968946063	0.0
13	4.002569649715117E-4	0.0	0.0	7.379737791662247	0.0
14	4.002569649715117E-4	0.0	0.0	7.545143982436724	0.0
15	4.002569649715117E-4	0.0	0.0	7.793703557684033	0.0
16	4.002569649715117E-4	0.0	0.0	8.206368488569662	0.0
17	4.002569649715117E-4	0.0	0.0	8.669565861282944	0.0
18	4.002569649715117E-4	0.0	0.0	9.147472677458929	0.0
19	4.002569649715117E-4	0.0	0.0	9.482287628657598	0.0
20	4.002569649715117E-4	0.0	0.0	9.806195577560795	0.0
21	5.003212062143896E-4	0.0	0.0	10.184238280976388	0.0
22	5.003212062143896E-4	0.0	0.0	10.56998593096768	0.0
23	5.003212062143896E-4	0.0	0.0	10.947027991970845	0.0
24	6.003854474572676E-4	0.0	0.0	11.285945577060472	0.0
25	7.004496887001455E-4	0.0	0.0	11.600347423045596	0.0
26	7.004496887001455E-4	0.0	0.0	11.883028904556726	0.0
27	7.004496887001455E-4	0.0	0.0	12.1735153968848	0.0
28	7.004496887001455E-4	0.0	0.0	12.471006386099877	0.0
29	7.004496887001455E-4	0.0	0.0	12.75869107967315	0.0
30	7.004496887001455E-4	0.0	0.0	13.104513097408537	0.0
31	7.004496887001455E-4	0.0	0.0	13.401703893899883	0.0
32	7.004496887001455E-4	0.0	0.0	13.73291653241381	0.0
33	7.004496887001455E-4	0.0	0.0	14.04411632267916	0.0
34	7.004496887001455E-4	0.0	0.0	14.356416819598182	0.0
35	7.004496887001455E-4	0.0	0.0	14.68422727390985	0.0
36	8.005139299430234E-4	0.0	0.0	15.008235287054289	0.0
37	8.005139299430234E-4	0.0	0.0	15.340148375256915	0.0
38	8.005139299430234E-4	0.0	0.0	15.650347523109836	0.0
39	8.005139299430234E-4	0.0	0.0	15.993567870572909	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	25	3.8904393E-5	45.0	1
CATACCG	20	7.032846E-4	45.0	29
TATAGCG	55	1.8189894E-12	45.0	1
ATCGCTT	20	7.032846E-4	45.0	44
TACCGTG	20	7.032846E-4	45.0	17
CGTTTTT	3285	0.0	40.0	1
CGTAAGG	85	0.0	39.705883	2
TCGGCGT	245	0.0	38.57143	4
GCGATCG	35	6.2474683E-6	38.57143	9
GATCGCG	30	1.13981514E-4	37.500004	1
TAACGCC	30	1.13981514E-4	37.500004	12
CGTTAGG	145	0.0	37.24138	2
CGCTAGT	365	0.0	36.369865	4
ATAGCGG	180	0.0	36.25	2
TACAGCG	75	1.8189894E-12	36.000004	1
TTTGTCG	25	0.0021069774	36.0	1
ATTCGCA	25	0.0021069774	36.0	30
TCGATTA	25	0.0021069774	36.0	41
GTTCGGA	25	0.0021069774	36.0	12
CCGGTAT	25	0.0021069774	36.0	42
>>END_MODULE