##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	625775
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4378762334705	33.0	31.0	34.0	31.0	34.0
2	32.53949582517678	34.0	31.0	34.0	31.0	34.0
3	32.55349286884263	34.0	31.0	34.0	31.0	34.0
4	36.02627621749031	37.0	35.0	37.0	35.0	37.0
5	36.06667572210459	37.0	35.0	37.0	35.0	37.0
6	36.093955495185966	37.0	36.0	37.0	35.0	37.0
7	36.215190763453315	37.0	37.0	37.0	35.0	37.0
8	35.902735008589346	37.0	37.0	37.0	35.0	37.0
9	37.784077344093326	39.0	38.0	39.0	35.0	39.0
10	37.67883025048939	39.0	37.0	39.0	35.0	39.0
11	37.6129631257241	39.0	37.0	39.0	35.0	39.0
12	37.47724981023531	39.0	37.0	39.0	35.0	39.0
13	37.41380847748791	39.0	37.0	39.0	35.0	39.0
14	38.68137908992849	40.0	38.0	41.0	35.0	41.0
15	38.76562981902441	40.0	38.0	41.0	35.0	41.0
16	38.81053733370621	40.0	38.0	41.0	35.0	41.0
17	38.8268019655627	40.0	38.0	41.0	35.0	41.0
18	38.8037074028205	40.0	38.0	41.0	35.0	41.0
19	38.77776197515081	40.0	38.0	41.0	35.0	41.0
20	38.717877831488956	40.0	38.0	41.0	35.0	41.0
21	38.653774919100314	40.0	38.0	41.0	35.0	41.0
22	38.62583037034078	40.0	38.0	41.0	35.0	41.0
23	38.60001278414766	40.0	38.0	41.0	35.0	41.0
24	38.534418920538535	40.0	38.0	41.0	35.0	41.0
25	38.4512564420119	40.0	37.0	41.0	35.0	41.0
26	38.431119811433824	40.0	37.0	41.0	35.0	41.0
27	38.368268147497105	40.0	37.0	41.0	35.0	41.0
28	38.309812632335905	40.0	37.0	41.0	34.0	41.0
29	38.299674803243974	40.0	37.0	41.0	34.0	41.0
30	38.21749830210539	40.0	37.0	41.0	34.0	41.0
31	38.11403299908114	40.0	37.0	41.0	34.0	41.0
32	38.04195118053614	40.0	37.0	41.0	34.0	41.0
33	37.9855986576645	40.0	37.0	41.0	34.0	41.0
34	37.879017218648876	40.0	36.0	41.0	34.0	41.0
35	37.81186209100715	40.0	36.0	41.0	33.0	41.0
36	37.75924094123287	40.0	36.0	41.0	33.0	41.0
37	37.69359913707003	40.0	36.0	41.0	33.0	41.0
38	37.62991170948025	40.0	36.0	41.0	33.0	41.0
39	37.54816986936199	40.0	35.0	41.0	33.0	41.0
40	37.40573049418721	40.0	35.0	41.0	33.0	41.0
41	37.32119851384284	40.0	35.0	41.0	33.0	41.0
42	37.32409252526867	40.0	35.0	41.0	33.0	41.0
43	37.30754504414526	40.0	35.0	41.0	33.0	41.0
44	37.25116695297831	39.0	35.0	41.0	33.0	41.0
45	37.15901721864888	39.0	35.0	41.0	33.0	41.0
46	37.004864368183455	39.0	35.0	41.0	32.0	41.0
47	36.924448883384606	39.0	35.0	41.0	32.0	41.0
48	36.870518956493946	39.0	35.0	41.0	32.0	41.0
49	36.78168670848148	39.0	35.0	41.0	32.0	41.0
50	36.71811433822061	39.0	35.0	41.0	32.0	41.0
51	35.875979385561905	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	11.0
12	18.0
13	15.0
14	22.0
15	38.0
16	50.0
17	60.0
18	106.0
19	204.0
20	337.0
21	522.0
22	792.0
23	1246.0
24	1783.0
25	2550.0
26	3488.0
27	4285.0
28	4661.0
29	5342.0
30	6419.0
31	8067.0
32	10806.0
33	15705.0
34	32484.0
35	50597.0
36	40451.0
37	60838.0
38	115234.0
39	259110.0
40	521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60045543526027	20.911030322400222	23.54919899324837	12.939315249091127
2	32.01102632735408	25.801925612240822	24.409412328712396	17.7776357316927
3	32.61795373736567	24.317366465582676	25.785945427669688	17.278734369381965
4	29.612081019535776	24.562182893212416	25.635891494546765	20.189844592705043
5	26.304981822540054	29.0815388917742	23.911789381167353	20.701689904518396
6	25.824297870640407	35.122368263353444	23.369901322360274	15.683432543645878
7	79.13259558147897	6.721745036155167	9.19084335424074	4.954816028125125
8	79.94183212816108	6.280212536454796	8.262394630657983	5.5155607047261395
9	73.28608525428469	8.625943829651233	10.748431944388956	7.339538971675124
10	41.358155886700494	24.624985018576965	18.044984219567738	15.971874875154807
11	30.45903080180576	25.67552235228317	23.29287683272742	20.572570013183654
12	29.140825376533098	22.496104830010786	27.80440254084935	20.558667252606767
13	25.47337301745835	24.331748631696694	28.361471774999004	21.833406575845952
14	20.33830050737086	28.48883384603092	28.231233270744276	22.941632375853942
15	20.004154847988495	27.045024170029166	32.130078702409016	20.820742279573327
16	22.606527905397307	25.335464024609482	30.58719188206624	21.47081618792697
17	22.809795853142102	25.63525228716392	27.064360193360233	24.490591666333746
18	23.43957492709041	26.1122607966122	28.196236666533498	22.251927609763893
19	24.739562941951977	26.841436618592944	26.831209300467417	21.587791138987654
20	26.561303983061002	25.370141025128838	27.234549159042786	20.834005832767367
21	25.909152650713114	26.559066757221046	27.085134433302706	20.446646158763134
22	23.816068075586273	25.360552914386158	26.73165275058927	24.091726259438296
23	23.428069194199193	26.65063321481363	27.12124965043346	22.800047940553714
24	23.555431265231114	25.491590427869443	28.16124006232272	22.791738244576727
25	23.400583276736846	26.99021213695018	26.819863369421913	22.789341216891053
26	21.940633614318244	27.821181734649038	26.914625863928727	23.32355878710399
27	23.29303663457313	27.62526467180696	26.972314330230514	22.1093843633894
28	22.010307219048382	26.668211417841874	28.725660181375094	22.59582118173465
29	22.24681395070113	25.591626383284726	28.390875314609882	23.77068435140426
30	22.660381127402022	26.59550157804323	27.160560904478448	23.583556390076303
31	25.12148935320203	26.664695777236226	25.258918940513762	22.95489592904798
32	25.409771882865247	26.582078223003474	26.19727537853062	21.810874515600656
33	24.7977308137909	27.15129239742719	24.857177100395507	23.193799688386402
34	23.4084135671767	25.7324118093564	27.560385122448167	23.29878950101874
35	24.925732092205667	24.409252526866684	26.867803923135313	23.79721145779234
36	24.677080420278855	27.499820222923578	25.360552914386158	22.46254644241141
37	24.409412328712396	27.134992609164637	26.764731732651516	21.690863329471455
38	25.192601174543565	26.741560465023372	25.067795933043023	22.998042427390036
39	25.22008709200591	25.26003755343374	27.133714194398944	22.3861611601614
40	25.282889217370464	24.275977787543447	29.14290280052735	21.298230194558748
41	21.88054812033079	25.58091965962207	28.215093284327438	24.323438935719707
42	22.855978586552673	26.195038152690664	27.07346889856578	23.875514362190884
43	24.153889177419998	25.50836962166913	26.931245255882708	23.406495945028166
44	24.03132116175942	24.93084575126843	26.81379089928489	24.224042187687267
45	24.13183652271184	24.446166753226002	26.3198433941912	25.10215332987096
46	24.462786145179976	26.08381606807559	26.298909352403022	23.154488434341417
47	23.484159642043867	25.371579241740243	29.053094163237585	22.091166952978305
48	23.293835643801685	24.481802564819624	28.11729455475211	24.107067236626584
49	22.917022891614398	24.43673844432903	29.321721065878307	23.32451759817826
50	22.75945827174304	24.146058886980146	28.648635691742243	24.44584714953458
51	22.25672166513523	24.45911070272862	26.977587791138983	26.306579840997163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	881.5
2	1395.0
3	1999.0
4	2603.0
5	1908.5
6	1214.0
7	1172.0
8	1130.0
9	1163.0
10	1196.0
11	1205.0
12	1214.0
13	1128.0
14	1042.0
15	1145.5
16	1249.0
17	1235.0
18	1221.0
19	1386.5
20	1552.0
21	1817.5
22	2083.0
23	2233.5
24	2384.0
25	2924.5
26	4412.5
27	5360.0
28	6270.0
29	7180.0
30	8878.5
31	10577.0
32	11247.5
33	11918.0
34	13464.5
35	15011.0
36	16587.0
37	18163.0
38	19084.5
39	20006.0
40	22383.0
41	24760.0
42	27659.0
43	30558.0
44	32602.0
45	34646.0
46	38678.5
47	42711.0
48	47373.5
49	52036.0
50	54471.5
51	56907.0
52	57347.5
53	57788.0
54	50941.5
55	44095.0
56	40145.5
57	36196.0
58	33860.5
59	31525.0
60	29820.0
61	28115.0
62	25993.0
63	23871.0
64	21220.5
65	18570.0
66	15270.0
67	11970.0
68	10354.0
69	8738.0
70	7053.0
71	5368.0
72	4377.5
73	3387.0
74	2845.5
75	1622.5
76	941.0
77	754.0
78	567.0
79	402.5
80	238.0
81	179.0
82	120.0
83	70.0
84	20.0
85	14.0
86	8.0
87	6.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	625775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.374222625686546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34395460811635	19.080155644570226
2	10.129597810678456	5.343205355350012
3	3.612773029698609	2.858522405440416
4	1.7445718150381686	1.8404690174525882
5	1.0852311452949803	1.4311063913169295
6	0.75037448925971	1.1874326299422855
7	0.5840030985449297	1.0781839414580314
8	0.519256000910242	1.0955978694184334
9	0.4386693834264489	1.0412607579805633
>10	8.3408313253787	54.1338624968268
>50	0.3742893130473235	5.9526330904353255
>100	0.0684973906230396	3.151650172176886
>500	0.00550425460363711	0.8965080944244401
>1k	0.0024463353793942708	0.9094121332070876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1831	0.2925971794974232	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1601	0.2558427549838201	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.19192201669929287	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	1005	0.16060085493987455	Illumina Single End Adapter 1 (95% over 21bp)
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	763	0.12192880827773561	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	724	0.11569653629499421	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	653	0.10435060524949062	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5980184571131798E-4	0.0	0.0	0.1661939195397707	0.0
2	1.5980184571131798E-4	0.0	0.0	0.8768327274180017	0.0
3	1.5980184571131798E-4	0.0	0.0	1.1771003955095682	0.0
4	1.5980184571131798E-4	0.0	0.0	1.5588670049139068	0.0
5	1.5980184571131798E-4	0.0	0.0	2.6597419200191763	0.0
6	1.5980184571131798E-4	0.0	0.0	3.3007071231672724	0.0
7	1.5980184571131798E-4	0.0	0.0	3.8499460668770724	0.0
8	1.5980184571131798E-4	0.0	0.0	4.637129958851025	0.0
9	1.5980184571131798E-4	0.0	0.0	4.980064719747513	0.0
10	1.5980184571131798E-4	0.0	0.0	5.904038991650354	0.0
11	1.5980184571131798E-4	0.0	0.0	7.111981143382206	0.0
12	1.5980184571131798E-4	0.0	0.0	8.17338500259678	0.0
13	1.5980184571131798E-4	0.0	0.0	8.585993368223402	0.0
14	1.5980184571131798E-4	0.0	0.0	8.74819224162039	0.0
15	1.5980184571131798E-4	0.0	0.0	9.066038112740202	0.0
16	1.5980184571131798E-4	0.0	0.0	9.683991850105869	0.0
17	1.5980184571131798E-4	0.0	0.0	10.341097039670808	0.0
18	1.5980184571131798E-4	0.0	0.0	11.063880787823098	0.0
19	1.5980184571131798E-4	0.0	0.0	11.529703168071592	0.0
20	1.5980184571131798E-4	0.0	0.0	11.9562142942751	0.0
21	1.5980184571131798E-4	0.0	0.0	12.504015021373498	0.0
22	1.5980184571131798E-4	0.0	0.0	13.077224241939994	0.0
23	1.5980184571131798E-4	0.0	0.0	13.60153409771883	0.0
24	1.5980184571131798E-4	0.0	0.0	14.042906795573488	0.0
25	1.5980184571131798E-4	0.0	0.0	14.442411409851784	0.0
26	1.5980184571131798E-4	0.0	0.0	14.812512484519196	0.0
27	1.5980184571131798E-4	0.0	0.0	15.175422476129599	0.0
28	1.5980184571131798E-4	0.0	0.0	15.55862730214534	0.0
29	1.5980184571131798E-4	0.0	0.0	15.937357676481163	0.0
30	1.5980184571131798E-4	0.0	0.0	16.356997323319085	0.0
31	1.5980184571131798E-4	0.0	0.0	16.75298629699173	0.0
32	1.5980184571131798E-4	0.0	0.0	17.160800607247015	0.0
33	1.5980184571131798E-4	0.0	0.0	17.540489792657105	0.0
34	1.5980184571131798E-4	0.0	0.0	17.9329631257241	0.0
35	1.5980184571131798E-4	0.0	0.0	18.349246933802085	0.0
36	1.5980184571131798E-4	0.0	0.0	18.72430186568655	0.0
37	1.5980184571131798E-4	0.0	0.0	19.120610443050616	0.0
38	1.5980184571131798E-4	0.0	0.0	19.47521073868403	0.0
39	1.5980184571131798E-4	0.0	0.0	19.8545803204027	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCGTC	30	2.1639098E-6	45.000004	28
GCATCGC	30	2.1639098E-6	45.000004	22
CTCGTAT	20	7.03082E-4	45.0	39
ATTACGG	20	7.03082E-4	45.0	2
TAGCGCG	20	7.03082E-4	45.0	1
ATCGTAG	20	7.03082E-4	45.0	1
CGTTAGT	20	7.03082E-4	45.0	23
TGCGCGT	25	3.888762E-5	45.0	37
AGTTGCG	40	6.8066583E-9	45.0	1
CGGTCTA	25	3.888762E-5	45.0	31
TATCGCG	25	3.888762E-5	45.0	1
CGCCCGA	20	7.03082E-4	45.0	35
ATCGACT	25	3.888762E-5	45.0	41
GTACGAG	25	3.888762E-5	45.0	1
GTTGCGT	25	3.888762E-5	45.0	26
CGGACCA	20	7.03082E-4	45.0	37
CTAGCGG	75	0.0	42.0	2
TGAACGG	60	3.6379788E-12	41.250004	2
GCGTTAG	40	3.4548611E-7	39.375	1
ACTAAGG	80	0.0	39.375	2
>>END_MODULE