FastQCFastQC Report
Sat 18 Jun 2016
SRR3551197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380109
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC16550.43540142432828477No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC16210.4264566216532626No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.27571038833597733No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT10150.2670286680925735Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6240.16416343732981856No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC5380.14153834821064484No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC5340.14048601848417167No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG5280.13890752389446184No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC5060.13311971039885928No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC4760.1252272374503103No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4380.11523010504881494No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA4110.10812687939512086No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC4020.1057591375105562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTG302.1616543E-645.00000418
GTCTTAC302.1616543E-645.00000431
TATACGG302.1616543E-645.0000042
CGGGTAC207.027323E-445.06
ACACGCG253.8858623E-545.036
CGTATGG253.8858623E-545.02
CACGCCC207.027323E-445.044
ATCGTTA207.027323E-445.033
ATCGTAG207.027323E-445.01
TAGTTAG207.027323E-445.01
CAGGGTA502.1827873E-1145.05
CGTTAAG207.027323E-445.035
AACGGCA253.8858623E-545.040
GTTAACG253.8858623E-545.01
ACTACGA207.027323E-445.030
TACTAGA207.027323E-445.030
TTACGCG502.1827873E-1145.01
AACTCGA207.027323E-445.025
TCCCCGT207.027323E-445.017
AATAGCG207.027323E-445.01