##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	380109
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36264334703993	33.0	31.0	34.0	30.0	34.0
2	32.45804229839336	34.0	31.0	34.0	31.0	34.0
3	32.47665801125466	34.0	31.0	34.0	30.0	34.0
4	35.976782975409684	37.0	35.0	37.0	35.0	37.0
5	36.02061777016593	37.0	35.0	37.0	35.0	37.0
6	36.05300584832246	37.0	35.0	37.0	35.0	37.0
7	36.19282889907895	37.0	37.0	37.0	35.0	37.0
8	35.83624434043919	37.0	35.0	37.0	35.0	37.0
9	37.73551796984549	39.0	38.0	39.0	35.0	39.0
10	37.6603000718215	39.0	37.0	39.0	35.0	39.0
11	37.57632415964894	39.0	37.0	39.0	35.0	39.0
12	37.361769913366956	39.0	37.0	39.0	35.0	39.0
13	37.237937012804224	39.0	37.0	39.0	34.0	39.0
14	38.473545746088625	40.0	38.0	41.0	34.0	41.0
15	38.57031009526215	40.0	38.0	41.0	35.0	41.0
16	38.63816431602514	40.0	38.0	41.0	35.0	41.0
17	38.65232867414347	40.0	38.0	41.0	35.0	41.0
18	38.64685655956581	40.0	38.0	41.0	35.0	41.0
19	38.634623226495556	40.0	37.0	41.0	35.0	41.0
20	38.55333601677414	40.0	37.0	41.0	35.0	41.0
21	38.49230878511164	40.0	37.0	41.0	35.0	41.0
22	38.44701388285991	40.0	37.0	41.0	35.0	41.0
23	38.425522679021014	40.0	37.0	41.0	35.0	41.0
24	38.36290906029586	40.0	37.0	41.0	34.0	41.0
25	38.29076133424886	40.0	37.0	41.0	34.0	41.0
26	38.24717646780265	40.0	37.0	41.0	34.0	41.0
27	38.20807189516691	40.0	36.0	41.0	34.0	41.0
28	38.13641876409135	40.0	36.0	41.0	34.0	41.0
29	38.1337432157618	40.0	36.0	41.0	34.0	41.0
30	38.04979624265671	40.0	36.0	41.0	34.0	41.0
31	37.95193220891902	40.0	36.0	41.0	34.0	41.0
32	37.857151501279894	40.0	36.0	41.0	34.0	41.0
33	37.8231112654528	40.0	36.0	41.0	34.0	41.0
34	37.71172479472993	40.0	36.0	41.0	34.0	41.0
35	37.639103520306016	40.0	35.0	41.0	33.0	41.0
36	37.5685790128621	40.0	35.0	41.0	33.0	41.0
37	37.48161711509067	40.0	35.0	41.0	33.0	41.0
38	37.410442794040655	40.0	35.0	41.0	33.0	41.0
39	37.36749458707897	40.0	35.0	41.0	33.0	41.0
40	37.25464011638767	39.0	35.0	41.0	33.0	41.0
41	37.17477355179698	39.0	35.0	41.0	33.0	41.0
42	37.148525817594425	39.0	35.0	41.0	33.0	41.0
43	37.063076643804806	39.0	35.0	41.0	33.0	41.0
44	36.99157346971526	39.0	35.0	41.0	33.0	41.0
45	36.959240638869375	39.0	35.0	41.0	32.0	41.0
46	36.85233183113265	39.0	35.0	41.0	32.0	41.0
47	36.79579015492924	39.0	35.0	41.0	32.0	41.0
48	36.7646517183229	39.0	35.0	41.0	32.0	41.0
49	36.67576668797634	39.0	35.0	41.0	32.0	41.0
50	36.601798431502544	39.0	35.0	41.0	32.0	41.0
51	35.79301726609999	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	8.0
10	6.0
11	8.0
12	5.0
13	13.0
14	10.0
15	13.0
16	35.0
17	44.0
18	82.0
19	151.0
20	211.0
21	351.0
22	546.0
23	793.0
24	1141.0
25	1730.0
26	2321.0
27	2722.0
28	3180.0
29	3675.0
30	4136.0
31	5380.0
32	7308.0
33	10168.0
34	20933.0
35	35848.0
36	23968.0
37	35654.0
38	67260.0
39	152079.0
40	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2939209542526	20.646446150972483	23.12599806897495	13.933634825799967
2	32.73297922438038	24.974941398388356	24.491658971505544	17.800420405725724
3	32.24127815968578	24.34038657332502	26.36533204949107	17.053003217498137
4	29.879850253479923	25.28222167851853	25.16751773833295	19.670410329668595
5	25.70499514612914	29.25371406622837	24.256463277638783	20.78482751000371
6	25.249599456997863	34.381716823332255	24.281456108642523	16.087227611027362
7	77.15102773151912	7.058238557887344	10.350715189590353	5.440018521003186
8	76.62381053855604	6.96589662438932	9.954776130004813	6.45551670704982
9	70.95990886824569	8.557545335680029	12.670312989168897	7.812232806905388
10	42.03162776992915	23.660581570023336	19.38838596297378	14.919404697073734
11	32.79033119447317	23.934187298906366	23.755028162974305	19.520453343646167
12	30.783538406088777	22.154697731440194	27.65522521171559	19.406538650755444
13	24.65845323315154	26.35323025763662	29.216093278506953	19.772223230704878
14	19.92191713429569	29.541263163987175	28.281361398967135	22.25545830275
15	18.423399603797858	26.99436214349042	34.610072373976934	19.972165878734785
16	20.611982352430484	24.640563627801498	32.55566166546964	22.19179235429837
17	21.006605999857936	25.23907615973313	27.754144206004067	26.000173634404867
18	21.990271211678756	25.841798010570656	29.61703090429324	22.55089987345735
19	24.366431734055233	26.883341357347497	27.161682569999655	21.58854433859761
20	26.431102657395645	25.88441736449282	27.809917681507144	19.874562296604395
21	25.20329694903304	26.674980071505804	27.864638827283756	20.257084152177402
22	22.278346474300793	25.070966485929034	26.924119134248336	25.726567905521836
23	22.452507044032107	26.20800875538332	27.900418037983844	23.439066162600728
24	23.471688384121396	24.05704679447211	29.680170687881635	22.791094133524858
25	21.774017452888515	26.980681857046267	27.082757840514166	24.16254284955105
26	20.566469091760524	28.641258165420968	27.349260343743502	23.443012399075002
27	23.254119213173063	27.74256857901286	28.017752802485603	20.985559405328473
28	19.40995872236648	26.601580073084303	31.741421539611007	22.247039664938214
29	21.512513515859926	26.240894059335613	29.568360654443858	22.678231770360608
30	23.652162932211553	26.36691054408078	27.934092589230985	22.04683393447669
31	24.369062558371414	26.70681304573162	26.775740642815617	22.14838375308135
32	24.607415241417595	27.848064634091802	26.864925587134213	20.67959453735639
33	26.162495494713355	26.428734915511075	26.061998005835168	21.346771583940395
34	21.566971579204914	26.14039657045742	29.35289614294847	22.939735707389197
35	24.79604534488791	24.30934284639406	28.02169903895988	22.87291276975815
36	26.192486891917845	26.46635570323249	26.690238852539665	20.650918552309996
37	23.267799499617215	26.690501934971284	30.634633749792822	19.40706481561868
38	23.58876006619154	25.78181521616168	27.808076104485817	22.82134861316096
39	25.119110570915186	23.971545004196166	29.320273921427802	21.58907050346085
40	25.31642239462891	23.910772962492338	29.510745601919446	21.2620590409593
41	22.052095583109054	25.084383689941568	28.35028899605113	24.51323173089824
42	24.28171919107414	24.779997316559196	27.680218042719325	23.25806544964734
43	25.571086188435423	23.57586902704224	28.02432986327606	22.828714921246274
44	22.718746464829824	25.211978669276448	28.738861747551358	23.33041311834237
45	23.433015266673507	24.637406638622082	27.421344930006917	24.508233164697497
46	24.125974391556106	25.60397149238771	28.00433559847307	22.265718517583114
47	22.997876924776843	24.48850198232612	30.647261706510502	21.866359386386534
48	23.43853999773749	22.805563667263865	29.680433770313254	24.07546256468539
49	23.007874057178334	22.495652562817508	30.794587868216748	23.701885511787406
50	21.352559397436	23.892094109847438	30.457842355745324	24.297504136971238
51	21.673256881578705	23.44327548150662	26.950427377410165	27.93304025950451
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	200.0
1	609.0
2	1018.0
3	1510.5
4	2003.0
5	1425.0
6	847.0
7	816.0
8	785.0
9	769.5
10	754.0
11	816.5
12	879.0
13	843.0
14	807.0
15	796.5
16	786.0
17	815.5
18	845.0
19	803.5
20	762.0
21	1292.5
22	1823.0
23	1714.5
24	1606.0
25	2002.0
26	2706.0
27	3014.0
28	3490.0
29	3966.0
30	5110.0
31	6254.0
32	6871.0
33	7488.0
34	8757.0
35	10026.0
36	9880.0
37	9734.0
38	10567.0
39	11400.0
40	13455.0
41	15510.0
42	17899.0
43	20288.0
44	23285.0
45	26282.0
46	29788.5
47	33295.0
48	35236.5
49	37178.0
50	38005.0
51	38832.0
52	33105.5
53	27379.0
54	24196.0
55	21013.0
56	20100.0
57	19187.0
58	18038.0
59	16889.0
60	16319.0
61	15749.0
62	14700.5
63	13652.0
64	11757.5
65	9863.0
66	8287.5
67	6712.0
68	5304.0
69	3896.0
70	3472.0
71	3048.0
72	2345.0
73	1642.0
74	1476.5
75	852.5
76	394.0
77	428.5
78	463.0
79	284.5
80	106.0
81	62.0
82	18.0
83	11.5
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	380109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.110152899362642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.65704478305122	18.884131345862695
2	11.859214133431069	6.430102168471976
3	4.269758316921174	3.47261402445175
4	2.133617888809659	2.313708287777807
5	1.2937116958547152	1.7536360941157534
6	0.8914036287726502	1.4499653202643947
7	0.6927575383438074	1.3146533950680688
8	0.5709757723303442	1.2383392391765846
9	0.4811369086646076	1.173932564348177
>10	7.725142151200921	50.31321234711764
>50	0.34438222536116286	5.740849536716219
>100	0.07187107311885138	3.7312545211767336
>500	0.004991046744364679	0.7387819004070556
>1k	0.0039928373954917435	1.444819255045154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1655	0.43540142432828477	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1621	0.4264566216532626	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1048	0.27571038833597733	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	1015	0.2670286680925735	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	624	0.16416343732981856	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	538	0.14153834821064484	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	534	0.14048601848417167	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	528	0.13890752389446184	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	506	0.13311971039885928	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	476	0.1252272374503103	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	438	0.11523010504881494	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	411	0.10812687939512086	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	402	0.1057591375105562	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2749211410411224	0.0
2	2.63082431618299E-4	0.0	0.0	1.403544772683625	0.0
3	2.63082431618299E-4	0.0	0.0	1.8713053361009606	0.0
4	2.63082431618299E-4	0.0	0.0	2.381159088577224	0.0
5	2.63082431618299E-4	0.0	0.0	3.9941174768290146	0.0
6	2.63082431618299E-4	0.0	0.0	4.830193444511969	0.0
7	2.63082431618299E-4	0.0	0.0	5.639961169033093	0.0
8	2.63082431618299E-4	0.0	0.0	6.7675324709491225	0.0
9	2.63082431618299E-4	0.0	0.0	7.219771170900978	0.0
10	2.63082431618299E-4	0.0	0.0	8.37496612813693	0.0
11	2.63082431618299E-4	0.0	0.0	9.911630611219413	0.0
12	2.63082431618299E-4	0.0	0.0	11.178372519461522	0.0
13	2.63082431618299E-4	0.0	0.0	11.637714445067074	0.0
14	2.63082431618299E-4	0.0	0.0	11.843444906592582	0.0
15	2.63082431618299E-4	0.0	0.0	12.2520119228958	0.0
16	2.63082431618299E-4	0.0	0.0	13.021264952947707	0.0
17	2.63082431618299E-4	0.0	0.0	13.927057765009511	0.0
18	2.63082431618299E-4	0.0	0.0	14.895727278228087	0.0
19	2.63082431618299E-4	0.0	0.0	15.459249846754483	0.0
20	5.26164863236598E-4	0.0	0.0	15.994096430234485	0.0
21	5.26164863236598E-4	0.0	0.0	16.647067025511102	0.0
22	5.26164863236598E-4	0.0	0.0	17.342920057141505	0.0
23	5.26164863236598E-4	0.0	0.0	18.035616099592485	0.0
24	5.26164863236598E-4	0.0	0.0	18.608872718088758	0.0
25	5.26164863236598E-4	0.0	0.0	19.111886327342948	0.0
26	5.26164863236598E-4	0.0	0.0	19.550970905713886	0.0
27	5.26164863236598E-4	0.0	0.0	19.996895627306905	0.0
28	5.26164863236598E-4	0.0	0.0	20.442294184036683	0.0
29	5.26164863236598E-4	0.0	0.0	20.890849729945884	0.0
30	5.26164863236598E-4	0.0	0.0	21.39386333920007	0.0
31	5.26164863236598E-4	0.0	0.0	21.8471543688784	0.0
32	5.26164863236598E-4	0.0	0.0	22.328858301171508	0.0
33	5.26164863236598E-4	0.0	0.0	22.794251122704278	0.0
34	5.26164863236598E-4	0.0	0.0	23.241754338887002	0.0
35	5.26164863236598E-4	0.0	0.0	23.711356479325666	0.0
36	5.26164863236598E-4	0.0	0.0	24.169382992773127	0.0
37	5.26164863236598E-4	0.0	0.0	24.646088358865484	0.0
38	5.26164863236598E-4	0.0	0.0	25.081489783193767	0.0
39	5.26164863236598E-4	0.0	0.0	25.519785114269855	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGTG	30	2.1616543E-6	45.000004	18
GTCTTAC	30	2.1616543E-6	45.000004	31
TATACGG	30	2.1616543E-6	45.000004	2
CGGGTAC	20	7.027323E-4	45.0	6
ACACGCG	25	3.8858623E-5	45.0	36
CGTATGG	25	3.8858623E-5	45.0	2
CACGCCC	20	7.027323E-4	45.0	44
ATCGTTA	20	7.027323E-4	45.0	33
ATCGTAG	20	7.027323E-4	45.0	1
TAGTTAG	20	7.027323E-4	45.0	1
CAGGGTA	50	2.1827873E-11	45.0	5
CGTTAAG	20	7.027323E-4	45.0	35
AACGGCA	25	3.8858623E-5	45.0	40
GTTAACG	25	3.8858623E-5	45.0	1
ACTACGA	20	7.027323E-4	45.0	30
TACTAGA	20	7.027323E-4	45.0	30
TTACGCG	50	2.1827873E-11	45.0	1
AACTCGA	20	7.027323E-4	45.0	25
TCCCCGT	20	7.027323E-4	45.0	17
AATAGCG	20	7.027323E-4	45.0	1
>>END_MODULE