FastQCFastQC Report
Sat 18 Jun 2016
SRR3551195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394036
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC22850.5798962531342314No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG15400.3908272340598321No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC14900.3781380381487986No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC14400.3654488422377651No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC12740.3233207118131338No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12220.31012394806565896No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT11510.2921052898719914Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC8350.21190957171425961No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC6080.1543006222781675No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6060.15379305444172614No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC5710.14491061730400268No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC5530.1403425067760306No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3990.10125978337004742No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAGG207.0276356E-445.0000042
ACACGAA207.0276356E-445.00000432
TCCGCAT207.0276356E-445.00000426
CGAGTAC207.0276356E-445.0000049
CGACGGT207.0276356E-445.00000428
CGCATGT207.0276356E-445.00000428
GTTAGTC207.0276356E-445.00000423
TACGGGT207.0276356E-445.0000044
GACCCTA406.7957444E-945.00000412
AGAACCG207.0276356E-445.00000410
CTACGCC207.0276356E-445.00000414
CGAGGGT406.7957444E-945.0000044
AACGACT207.0276356E-445.00000427
ATGCGCG207.0276356E-445.0000041
CGATGAT207.0276356E-445.00000410
GAACCGC207.0276356E-445.00000411
GCTACGC207.0276356E-445.00000413
GAACACG207.0276356E-445.00000430
CGTTTGA207.0276356E-445.00000444
ACGACTT207.0276356E-445.00000413