##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551195_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394036
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.224659675765665	33.0	31.0	34.0	30.0	34.0
2	32.32303647382473	34.0	31.0	34.0	30.0	34.0
3	32.35151864296663	34.0	31.0	34.0	30.0	34.0
4	35.87601132891411	37.0	35.0	37.0	35.0	37.0
5	35.9324224182562	37.0	35.0	37.0	35.0	37.0
6	35.96386117004538	37.0	35.0	37.0	35.0	37.0
7	36.141306885665266	37.0	36.0	37.0	35.0	37.0
8	35.780484524256664	37.0	35.0	37.0	35.0	37.0
9	37.66951750601468	39.0	38.0	39.0	35.0	39.0
10	37.564699672111175	39.0	37.0	39.0	35.0	39.0
11	37.48108294673583	39.0	37.0	39.0	35.0	39.0
12	37.25647656559299	39.0	37.0	39.0	35.0	39.0
13	37.128929843973644	39.0	37.0	39.0	33.0	39.0
14	38.304588920809266	40.0	37.0	41.0	34.0	41.0
15	38.39383203565156	40.0	37.0	41.0	34.0	41.0
16	38.48472220812312	40.0	37.0	41.0	35.0	41.0
17	38.4982463531251	40.0	37.0	41.0	35.0	41.0
18	38.52096508948421	40.0	37.0	41.0	35.0	41.0
19	38.5161025896111	40.0	37.0	41.0	35.0	41.0
20	38.39862093818839	40.0	37.0	41.0	34.0	41.0
21	38.36522297455055	40.0	37.0	41.0	34.0	41.0
22	38.34747840298856	40.0	37.0	41.0	34.0	41.0
23	38.31405252311971	40.0	37.0	41.0	34.0	41.0
24	38.2641205372098	40.0	36.0	41.0	34.0	41.0
25	38.1892111380686	40.0	36.0	41.0	34.0	41.0
26	38.195294338588354	40.0	36.0	41.0	34.0	41.0
27	38.150856774507915	40.0	36.0	41.0	34.0	41.0
28	38.101972916180245	40.0	36.0	41.0	34.0	41.0
29	38.10406917134475	40.0	36.0	41.0	34.0	41.0
30	38.01253438772092	40.0	36.0	41.0	34.0	41.0
31	37.95602178481154	40.0	36.0	41.0	34.0	41.0
32	37.833682709194086	40.0	36.0	41.0	34.0	41.0
33	37.708156107563774	40.0	36.0	41.0	33.0	41.0
34	37.657069404825954	40.0	36.0	41.0	33.0	41.0
35	37.58891065790943	40.0	35.0	41.0	33.0	41.0
36	37.466076703651446	40.0	35.0	41.0	33.0	41.0
37	37.42766396978956	40.0	35.0	41.0	33.0	41.0
38	37.33517242079404	40.0	35.0	41.0	33.0	41.0
39	37.257194774081555	40.0	35.0	41.0	33.0	41.0
40	37.15738663472373	39.0	35.0	41.0	33.0	41.0
41	37.10048828025866	39.0	35.0	41.0	32.0	41.0
42	37.060649280776374	39.0	35.0	41.0	32.0	41.0
43	36.98509780832208	39.0	35.0	41.0	32.0	41.0
44	36.969137337705185	39.0	35.0	41.0	32.0	41.0
45	36.92812585652072	39.0	35.0	41.0	32.0	41.0
46	36.78691540874438	39.0	35.0	41.0	32.0	41.0
47	36.70864337268676	39.0	35.0	41.0	31.0	41.0
48	36.68957404907166	39.0	35.0	41.0	31.0	41.0
49	36.594369549990354	39.0	35.0	41.0	31.0	41.0
50	36.522457338923346	39.0	35.0	41.0	31.0	41.0
51	35.70945801906425	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	4.0
11	6.0
12	2.0
13	7.0
14	13.0
15	11.0
16	24.0
17	35.0
18	73.0
19	133.0
20	214.0
21	353.0
22	578.0
23	873.0
24	1326.0
25	1891.0
26	2540.0
27	3066.0
28	3436.0
29	3984.0
30	4732.0
31	6063.0
32	8111.0
33	11247.0
34	22770.0
35	37199.0
36	24793.0
37	36872.0
38	71438.0
39	151945.0
40	294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16601528794323	20.76967586717965	23.370707245023297	11.693601599853821
2	32.08996132333086	25.911591834248647	23.980550000507566	18.017896841912922
3	31.16111218264321	25.94915185414531	26.229075515942707	16.66066044726878
4	27.975362657219137	25.11394897928108	27.572100011166494	19.33858835233329
5	25.497670263630734	28.489528875534216	25.1743495518176	20.83845130901745
6	25.244647697164723	33.28147681937691	25.475844846663758	15.998030636794608
7	75.89611101523718	6.648377305626897	11.6859880823072	5.769523596828717
8	74.53329137439219	7.527484798343298	10.901034423250668	7.038189404013846
9	68.4546589651707	8.300003045407019	13.649514257580526	9.59582373184176
10	40.15546802830198	24.931478342080418	20.10780740846014	14.805246221157459
11	31.332670111360382	24.66322874052117	24.392695083697934	19.611406064420507
12	28.807773908982938	22.525860581266684	29.45898344313718	19.207382066613203
13	23.55393923397862	27.565247845374536	30.537818879493244	18.342994041153602
14	18.4571968043529	31.296125227136606	28.9854226517374	21.261255316773088
15	17.416428955729934	26.32906637972165	37.322224365286424	18.932280299261997
16	18.798282390441482	25.34387720918901	33.185800282207715	22.672040118161792
17	18.962734369448476	25.19211442609305	29.413809905693896	26.431341298764583
18	20.118212549107188	26.213087129094802	31.382157975413417	22.286542346384593
19	23.255743130069334	26.444030494675612	28.6440832817306	21.65614309352445
20	24.578464911835468	26.01488188896446	29.82722390847537	19.579429290724708
21	21.950532438660428	27.81700149224944	29.552883492878824	20.67958257621131
22	19.535270888954308	26.44149265549341	28.01114618968825	26.012090265864035
23	19.909348384411576	27.019612421200094	29.491467784669425	23.57957140971891
24	21.47494137591489	24.118608452019615	30.82814768193769	23.578302490127808
25	19.786516967992775	27.44267021287395	28.777066054878237	23.993746764255043
26	19.678405018830766	28.107076510775663	28.295130394177182	23.919388076216386
27	21.687866083302033	27.94871534580597	28.43877209189008	21.924646479001918
28	18.002162238983242	26.443522926839176	32.8860814747891	22.66823335938848
29	22.29212559258545	24.18306956724766	29.921377742135235	23.603427098031652
30	21.608177932980745	26.52930189119776	28.004547807814518	23.857972368006987
31	25.179171446263794	26.00346161264453	25.731405252311973	23.085961688779705
32	26.268665807185133	27.04879757179547	25.606289780629183	21.076246840390215
33	23.322742084479593	27.258676872163967	25.94077698484402	23.47780405851242
34	23.759504207737365	25.504522429422693	27.930189119775857	22.805784243064085
35	24.237886893583326	24.03815894994366	26.789937975210385	24.934016181262624
36	24.40462292785431	25.56492300195921	28.34664852957598	21.6838055406105
37	23.51485651057264	25.295658264727084	29.811743089463903	21.377742135236375
38	22.10331035742927	27.38226964033743	26.99220375803226	23.52221624420104
39	25.46467835426205	24.120384939447156	29.405181252474392	21.009755453816403
40	26.46255672070572	23.446081068734838	28.673014648407758	21.41834756215168
41	22.543371671623913	23.85315047356079	27.979169415992448	25.624308438822847
42	23.695804444263977	22.862124272909075	29.915794495934385	23.52627678689257
43	24.328487752388106	23.320204245297386	28.712351155731962	23.638956846582545
44	22.293902080012995	24.653331167710565	28.171030058167272	24.88173669410917
45	22.749190429300874	24.30716990325757	26.25318498817367	26.690454679267884
46	24.868793714279914	25.80728664385995	26.42753453999127	22.896385101868866
47	22.22817204519384	23.718644996903837	31.850135520612326	22.203047437289992
48	22.539311128932383	22.208884467409067	30.177191931701675	25.074612471956875
49	21.888101594778142	21.69091149032068	32.840907937345825	23.58007897755535
50	21.706646093250363	22.208884467409067	30.869260676689443	25.215208762651127
51	21.3571856378605	22.916434031408297	27.061740551624723	28.664639779106476
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	244.0
1	547.0
2	850.0
3	1523.5
4	2197.0
5	1537.5
6	878.0
7	895.0
8	912.0
9	899.5
10	887.0
11	907.5
12	928.0
13	868.0
14	808.0
15	842.5
16	877.0
17	884.0
18	891.0
19	989.5
20	1088.0
21	1149.5
22	1211.0
23	1360.0
24	1509.0
25	1878.0
26	2827.5
27	3408.0
28	4020.0
29	4632.0
30	5528.0
31	6424.0
32	6850.5
33	7277.0
34	8523.0
35	9769.0
36	10961.0
37	12153.0
38	12341.5
39	12530.0
40	13467.0
41	14404.0
42	17606.5
43	20809.0
44	23791.0
45	26773.0
46	28019.0
47	29265.0
48	35404.0
49	41543.0
50	42886.0
51	44229.0
52	42982.5
53	41736.0
54	34823.5
55	27911.0
56	23642.0
57	19373.0
58	17037.0
59	14701.0
60	13707.0
61	12713.0
62	11031.0
63	9349.0
64	8219.5
65	7090.0
66	6076.0
67	5062.0
68	4139.0
69	3216.0
70	2455.0
71	1694.0
72	1361.0
73	1028.0
74	947.5
75	588.5
76	310.0
77	244.0
78	178.0
79	101.0
80	24.0
81	26.0
82	28.0
83	17.0
84	6.0
85	5.0
86	4.0
87	3.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	394036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.403946342581662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.11058366261406	20.61527840062586
2	12.28199716586774	7.222783712898301
3	4.88570144969987	4.309767098185453
4	2.510561009537636	2.952816048568892
5	1.4555318535613893	2.1399190261018757
6	0.9208103250558246	1.6245274433781987
7	0.6479247814372974	1.333608185518787
8	0.49979998536696507	1.1756873561402672
9	0.36986891014731055	0.9788045028984609
>10	5.752456289966454	41.906728972161986
>50	0.48160904771543356	8.567529991261507
>100	0.07276107915125254	3.7150244501022756
>500	0.004331016616145985	0.8081566282856522
>1k	0.006063423262604379	2.6493681838724714
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	2285	0.5798962531342314	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG	1540	0.3908272340598321	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC	1490	0.3781380381487986	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC	1440	0.3654488422377651	No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	1274	0.3233207118131338	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1222	0.31012394806565896	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	1151	0.2921052898719914	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	835	0.21190957171425961	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC	608	0.1543006222781675	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	606	0.15379305444172614	No Hit
ACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	571	0.14491061730400268	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	553	0.1403425067760306	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	399	0.10125978337004742	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3032717822737009	0.0
2	0.0	0.0	0.0	1.6155884233927864	0.0
3	0.0	0.0	0.0	2.1581784405485793	0.0
4	0.0	0.0	0.0	2.89694342648895	0.0
5	0.0	0.0	0.0	5.112730816473622	0.0
6	0.0	0.0	0.0	6.1192378361368	0.0
7	0.0	0.0	0.0	7.188683267518704	0.0
8	0.0	0.0	0.0	8.672304053436742	0.0
9	0.0	0.0	0.0	9.300419251032901	0.0
10	0.0	0.0	0.0	11.059649372138587	0.0
11	0.0	0.0	0.0	13.050330426661523	0.0
12	0.0	0.0	0.0	14.958277923844522	0.0
13	0.0	0.0	0.0	15.58233257874915	0.0
14	0.0	0.0	0.0	15.856672994345693	0.0
15	0.0	0.0	0.0	16.436569247479927	0.0
16	0.0	0.0	0.0	17.487996020668163	0.0
17	0.0	0.0	0.0	18.63002365266118	0.0
18	0.0	0.0	0.0	19.953506786181972	0.0
19	0.0	0.0	0.0	20.740998284420712	0.0
20	0.0	0.0	0.0	21.434843516836025	0.0
21	0.0	0.0	0.0	22.33374615517364	0.0
22	0.0	0.0	0.0	23.232902577429474	0.0
23	0.0	0.0	0.0	24.112263854064096	0.0
24	0.0	0.0	0.0	24.813976387944248	0.0
25	0.0	0.0	0.0	25.459602675897635	0.0
26	0.0	0.0	0.0	26.00244647697165	0.0
27	0.0	0.0	0.0	26.573714076886375	0.0
28	0.0	0.0	0.0	27.122902475915907	0.0
29	0.0	0.0	0.0	27.684018719101807	0.0
30	0.0	0.0	0.0	28.334213117583165	0.0
31	2.5378391822067017E-4	0.0	0.0	28.97958562161833	0.0
32	2.5378391822067017E-4	0.0	0.0	29.583337563065303	0.0
33	2.5378391822067017E-4	0.0	0.0	30.11577622349227	0.0
34	2.5378391822067017E-4	0.0	0.0	30.656082185384076	0.0
35	2.5378391822067017E-4	0.0	0.0	31.272523322742085	0.0
36	2.5378391822067017E-4	0.0	0.0	31.830086591072895	0.0
37	2.5378391822067017E-4	0.0	0.0	32.39932391964186	0.0
38	2.5378391822067017E-4	0.0	0.0	32.94318285638876	0.0
39	2.5378391822067017E-4	0.0	0.0	33.4974469337827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGG	20	7.0276356E-4	45.000004	2
ACACGAA	20	7.0276356E-4	45.000004	32
TCCGCAT	20	7.0276356E-4	45.000004	26
CGAGTAC	20	7.0276356E-4	45.000004	9
CGACGGT	20	7.0276356E-4	45.000004	28
CGCATGT	20	7.0276356E-4	45.000004	28
GTTAGTC	20	7.0276356E-4	45.000004	23
TACGGGT	20	7.0276356E-4	45.000004	4
GACCCTA	40	6.7957444E-9	45.000004	12
AGAACCG	20	7.0276356E-4	45.000004	10
CTACGCC	20	7.0276356E-4	45.000004	14
CGAGGGT	40	6.7957444E-9	45.000004	4
AACGACT	20	7.0276356E-4	45.000004	27
ATGCGCG	20	7.0276356E-4	45.000004	1
CGATGAT	20	7.0276356E-4	45.000004	10
GAACCGC	20	7.0276356E-4	45.000004	11
GCTACGC	20	7.0276356E-4	45.000004	13
GAACACG	20	7.0276356E-4	45.000004	30
CGTTTGA	20	7.0276356E-4	45.000004	44
ACGACTT	20	7.0276356E-4	45.000004	13
>>END_MODULE