FastQCFastQC Report
Sat 18 Jun 2016
SRR3551194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655066
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC11590.17692873695169647No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.16334231970519No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC8710.1329637013674958No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC8390.1280786974136957No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG8250.12594150818390817No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA7750.11830868950609558No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC6930.10579086687448289No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6840.1044169595124766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGG406.8066583E-945.0000042
CGAACGC207.031061E-445.00000425
TTTACGC207.031061E-445.00000435
CGTGCGT207.031061E-445.00000412
GACACGC207.031061E-445.00000413
CGGTTGT207.031061E-445.00000416
TCGATAG207.031061E-445.0000041
CGCTAAG207.031061E-445.0000042
TATAGCG406.8066583E-945.0000041
GTACGAC207.031061E-445.00000435
ACCGGTA302.1640662E-644.99999641
GGTCCTA253.8889633E-544.9999969
GTCAACG253.8889633E-544.9999961
TCGTAAG302.1640662E-644.9999961
GGTTCGT253.8889633E-544.9999968
GTACGAG302.1640662E-644.9999961
CGTAAGG950.042.631582
AGACACG1500.042.024
AGTAGCG650.041.5384641
AGTACGG603.6379788E-1241.2499962