##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551194_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	655066
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41604662736274	33.0	31.0	34.0	31.0	34.0
2	32.51021271139091	34.0	31.0	34.0	31.0	34.0
3	32.527113298507324	34.0	31.0	34.0	31.0	34.0
4	36.01007990034592	37.0	35.0	37.0	35.0	37.0
5	36.04705480058498	37.0	35.0	37.0	35.0	37.0
6	36.081912356922814	37.0	36.0	37.0	35.0	37.0
7	36.210881040994344	37.0	37.0	37.0	35.0	37.0
8	35.885680221534926	37.0	36.0	37.0	35.0	37.0
9	37.760398188884785	39.0	38.0	39.0	35.0	39.0
10	37.656240745207356	39.0	37.0	39.0	35.0	39.0
11	37.578734051225375	39.0	37.0	39.0	35.0	39.0
12	37.41410941798231	39.0	37.0	39.0	35.0	39.0
13	37.35618243047266	39.0	37.0	39.0	35.0	39.0
14	38.6075861058275	40.0	38.0	41.0	35.0	41.0
15	38.69454680902383	40.0	38.0	41.0	35.0	41.0
16	38.734642005538376	40.0	38.0	41.0	35.0	41.0
17	38.73949037196252	40.0	38.0	41.0	35.0	41.0
18	38.73017375348438	40.0	38.0	41.0	35.0	41.0
19	38.717475796331975	40.0	38.0	41.0	35.0	41.0
20	38.664507087835425	40.0	38.0	41.0	35.0	41.0
21	38.59935029447414	40.0	38.0	41.0	35.0	41.0
22	38.584306008860175	40.0	38.0	41.0	35.0	41.0
23	38.55586917959412	40.0	37.0	41.0	35.0	41.0
24	38.49915123056303	40.0	37.0	41.0	35.0	41.0
25	38.40365550952118	40.0	37.0	41.0	34.0	41.0
26	38.394839603948306	40.0	37.0	41.0	35.0	41.0
27	38.342312072371335	40.0	37.0	41.0	34.0	41.0
28	38.29021197864032	40.0	37.0	41.0	34.0	41.0
29	38.26660672359732	40.0	37.0	41.0	34.0	41.0
30	38.18000934257006	40.0	37.0	41.0	34.0	41.0
31	38.14180861165134	40.0	37.0	41.0	34.0	41.0
32	38.07013644426669	40.0	36.0	41.0	34.0	41.0
33	38.0156335392159	40.0	36.0	41.0	34.0	41.0
34	37.94530169479106	40.0	36.0	41.0	34.0	41.0
35	37.86064915596291	40.0	36.0	41.0	34.0	41.0
36	37.81176095233152	40.0	36.0	41.0	34.0	41.0
37	37.76924920542358	40.0	36.0	41.0	34.0	41.0
38	37.67009125798011	40.0	35.0	41.0	33.0	41.0
39	37.609807256062744	40.0	35.0	41.0	33.0	41.0
40	37.51104621519053	40.0	35.0	41.0	33.0	41.0
41	37.46385402386935	40.0	35.0	41.0	33.0	41.0
42	37.43321741626035	40.0	35.0	41.0	33.0	41.0
43	37.36578146324187	40.0	35.0	41.0	33.0	41.0
44	37.287334405998784	39.0	35.0	41.0	33.0	41.0
45	37.21046886878574	39.0	35.0	41.0	33.0	41.0
46	37.054809744361634	39.0	35.0	41.0	33.0	41.0
47	37.01704561067129	39.0	35.0	41.0	33.0	41.0
48	36.96020095685015	39.0	35.0	41.0	33.0	41.0
49	36.8645037293952	39.0	35.0	41.0	32.0	41.0
50	36.811347253559184	39.0	35.0	41.0	32.0	41.0
51	36.0327356327454	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	11.0
11	12.0
12	18.0
13	15.0
14	17.0
15	31.0
16	43.0
17	71.0
18	124.0
19	198.0
20	297.0
21	505.0
22	747.0
23	1265.0
24	1721.0
25	2487.0
26	3478.0
27	4260.0
28	5073.0
29	5745.0
30	6718.0
31	8684.0
32	11374.0
33	16366.0
34	35469.0
35	56109.0
36	40740.0
37	62769.0
38	113745.0
39	276411.0
40	554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58532727999927	22.206770004854473	23.102710261256117	12.105192453890142
2	33.129791501925	24.330067504648387	23.9433889104304	18.59675208299622
3	33.054226597014654	23.78783206577658	25.260660757847305	17.897280579361468
4	30.335874553098467	25.380648667462513	24.567295509154803	19.716181270284217
5	25.483386406865872	30.31862438288661	23.595485035095702	20.602504175151818
6	26.609379818216787	34.41286832166529	23.204226749671026	15.773525110446887
7	79.65197399956647	6.500871667893006	8.546925042667457	5.300229289873081
8	80.01712804511301	6.342872321262286	8.220545716004189	5.4194539176205145
9	73.89927121847263	8.5418873823401	10.77616606570941	6.782675333477848
10	41.73594721753228	25.89571127184131	17.65287772529791	14.7154637853285
11	31.52277785749833	25.10556188231415	23.598843475313938	19.772816784873584
12	29.13095779661897	22.6993921223205	27.49539741033728	20.674252670723256
13	25.128155025600474	25.00923571060015	28.619100976084855	21.243508287714523
14	20.363902263283396	29.099052614545712	28.769620160411318	21.76742496175958
15	20.736078502013537	26.976976365740246	32.400399349073226	19.88654578317299
16	22.74412043977248	25.920136291610312	31.198841032811963	20.136902235805245
17	22.602302668738723	26.009592926514273	26.989799501118974	24.39830490362803
18	23.647082889357712	26.46191376136145	27.673852711024537	22.217150638256296
19	25.582613049677438	26.478705962452636	26.90400661918036	21.03467436868957
20	27.484253495067673	26.092180024608208	26.286053619024646	20.137512861299474
21	25.596504779671058	26.571673693948394	27.354342921171305	20.477478605209246
22	23.97132502679119	25.01060961796216	26.844470633493415	24.17359472175323
23	23.49564776678991	25.887009858548605	27.93336854607017	22.683973828591316
24	23.11477011476706	25.87846110162945	27.89077741784797	23.115991365755512
25	22.81739549907948	27.135433681491637	27.04200798087521	23.005162838553673
26	22.108764613031358	28.805799720944147	26.5266400637493	22.558795602275193
27	23.255977260306597	28.72183871548821	27.0343751621974	20.9878088620078
28	21.48058363584738	27.83017283754614	28.086635545120643	22.602607981485832
29	22.918454018373723	27.367318712923584	27.507915232968895	22.206312035733806
30	22.600318135882492	27.922529943547676	26.694714731034736	22.782437189535102
31	25.46567826753335	28.209371269459872	23.81469958752248	22.510250875484303
32	24.733690956331117	28.502166193940763	24.694000299206493	22.070142550521627
33	23.557473598080193	29.781884573462825	24.16336674472496	22.49727508373202
34	23.32787841225159	27.970769357591447	25.359734744285305	23.34161748587165
35	23.260709607886838	27.477231301884085	25.46598358028046	23.796075509948615
36	23.52251528853581	29.99361896358535	24.52516235005328	21.958703397825563
37	25.09411265429743	28.33409152665533	25.589329930113912	20.98246588893333
38	23.58540971444099	28.300049155352287	24.799791166080976	23.314749964125753
39	24.386703019237757	26.22850216619394	26.575948072407975	22.808846742160334
40	23.994376139198188	25.908076438099364	27.850018166108452	22.247529256593992
41	21.090546601411155	26.813176076914385	27.203976393218394	24.892300928456066
42	22.16051512366693	26.718376468935958	27.159706044887077	23.961402362510036
43	23.34650248982545	25.63131043284188	26.996821694302557	24.025365383030106
44	23.545261088195694	26.59228230437849	26.215831687188775	23.646624920237045
45	24.12230218023833	25.870828282951642	25.274705144214476	24.732164392595553
46	22.921201833097733	28.622764729050203	25.234403861595627	23.22162957625644
47	22.894181654978276	27.008881547813502	27.74834902131999	22.34858777588823
48	22.246613318352654	25.73893317619904	28.03778550558264	23.97666799986566
49	22.49040554692199	25.35469708395795	28.954944997908612	23.19995237121145
50	21.65293268159239	25.786714621122147	28.084651012264413	24.47570168502105
51	21.823755163601835	25.723514882469857	26.002265420583576	26.450464533344732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	322.0
1	858.0
2	1394.0
3	1910.0
4	2426.0
5	1804.5
6	1183.0
7	1167.0
8	1151.0
9	1185.0
10	1219.0
11	1185.0
12	1151.0
13	1197.0
14	1243.0
15	1278.5
16	1314.0
17	1299.5
18	1285.0
19	1209.0
20	1133.0
21	1579.0
22	2025.0
23	2184.0
24	2343.0
25	3199.5
26	5176.0
27	6296.0
28	7073.0
29	7850.0
30	9704.0
31	11558.0
32	12076.0
33	12594.0
34	13872.5
35	15151.0
36	16338.5
37	17526.0
38	19953.0
39	22380.0
40	25946.5
41	29513.0
42	32726.0
43	35939.0
44	39990.5
45	44042.0
46	48579.0
47	53116.0
48	56794.5
49	60473.0
50	58584.5
51	56696.0
52	52519.0
53	48342.0
54	44111.5
55	39881.0
56	35827.5
57	31774.0
58	30917.0
59	30060.0
60	31015.5
61	31971.0
62	27999.5
63	24028.0
64	22067.0
65	20106.0
66	16243.0
67	12380.0
68	10331.0
69	8282.0
70	6999.0
71	5716.0
72	4508.0
73	3300.0
74	2538.0
75	1536.5
76	1297.0
77	866.0
78	435.0
79	339.0
80	243.0
81	154.0
82	65.0
83	40.0
84	15.0
85	12.5
86	10.0
87	7.5
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	655066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.348119743979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83232887855877	18.208144739025887
2	10.602848685461137	5.375245562127184
3	3.8154083228360496	2.9014028111826686
4	1.9180507112208958	1.9447591641220543
5	1.179637982360533	1.495080241571029
6	0.7958342078493275	1.2103740478151654
7	0.6332778763315414	1.123668240832525
8	0.47570430523771806	0.9646567753513613
9	0.38701663625045785	0.8829129634729713
>10	7.4571793440527365	48.5773879220057
>50	0.8212012245856477	12.59794109299147
>100	0.07482055601780346	3.3962308668498107
>500	0.005474674830570985	0.9785018197143783
>1k	0.0012165944067935523	0.3436937529378111
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1159	0.17692873695169647	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1070	0.16334231970519	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	871	0.1329637013674958	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	839	0.1280786974136957	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	825	0.12594150818390817	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	775	0.11830868950609558	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	693	0.10579086687448289	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	684	0.1044169595124766	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10487492863314536	0.0
2	0.0	0.0	0.0	0.5619281110605648	0.0
3	0.0	0.0	0.0	0.8327405177493565	0.0
4	0.0	0.0	0.0	1.2252200541624814	0.0
5	0.0	0.0	0.0	2.301752800481173	0.0
6	0.0	0.0	0.0	2.9842794466511773	0.0
7	0.0	0.0	0.0	3.5381167699132607	0.0
8	0.0	0.0	0.0	4.407494817316118	0.0
9	0.0	0.0	0.0	4.789441063953861	0.0
10	0.0	0.0	0.0	5.584017488314155	0.0
11	0.0	0.0	0.0	6.793513936000342	0.0
12	0.0	0.0	0.0	7.673119960431468	0.0
13	0.0	0.0	0.0	8.055218863442768	0.0
14	0.0	0.0	0.0	8.220240403257076	0.0
15	0.0	0.0	0.0	8.498838284997237	0.0
16	0.0	0.0	0.0	9.111142999331365	0.0
17	0.0	0.0	0.0	9.782525730231763	0.0
18	0.0	0.0	0.0	10.563668393719107	0.0
19	0.0	0.0	0.0	11.011409537359594	0.0
20	0.0	0.0	0.0	11.448006765730476	0.0
21	0.0	0.0	0.0	11.992684706579185	0.0
22	0.0	0.0	0.0	12.550033126433062	0.0
23	0.0	0.0	0.0	13.079750742673257	0.0
24	0.0	0.0	0.0	13.538635801583352	0.0
25	0.0	0.0	0.0	13.947907539087664	0.0
26	0.0	0.0	0.0	14.300543762002608	0.0
27	0.0	0.0	0.0	14.657301707003569	0.0
28	0.0	0.0	0.0	15.049628587043138	0.0
29	1.5265637355625235E-4	0.0	0.0	15.43493327389912	0.0
30	1.5265637355625235E-4	0.0	0.0	15.909236626538394	0.0
31	1.5265637355625235E-4	0.0	0.0	16.32949962293876	0.0
32	1.5265637355625235E-4	0.0	0.0	16.745946210000213	0.0
33	1.5265637355625235E-4	0.0	0.0	17.173994681451944	0.0
34	1.5265637355625235E-4	0.0	0.0	17.56158921391127	0.0
35	1.5265637355625235E-4	0.0	0.0	17.99543862755814	0.0
36	1.5265637355625235E-4	0.0	0.0	18.41493834209072	0.0
37	1.5265637355625235E-4	0.0	0.0	18.838101809588654	0.0
38	1.5265637355625235E-4	0.0	0.0	19.256227616759226	0.0
39	1.5265637355625235E-4	0.0	0.0	19.672674203820684	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGG	40	6.8066583E-9	45.000004	2
CGAACGC	20	7.031061E-4	45.000004	25
TTTACGC	20	7.031061E-4	45.000004	35
CGTGCGT	20	7.031061E-4	45.000004	12
GACACGC	20	7.031061E-4	45.000004	13
CGGTTGT	20	7.031061E-4	45.000004	16
TCGATAG	20	7.031061E-4	45.000004	1
CGCTAAG	20	7.031061E-4	45.000004	2
TATAGCG	40	6.8066583E-9	45.000004	1
GTACGAC	20	7.031061E-4	45.000004	35
ACCGGTA	30	2.1640662E-6	44.999996	41
GGTCCTA	25	3.8889633E-5	44.999996	9
GTCAACG	25	3.8889633E-5	44.999996	1
TCGTAAG	30	2.1640662E-6	44.999996	1
GGTTCGT	25	3.8889633E-5	44.999996	8
GTACGAG	30	2.1640662E-6	44.999996	1
CGTAAGG	95	0.0	42.63158	2
AGACACG	150	0.0	42.0	24
AGTAGCG	65	0.0	41.538464	1
AGTACGG	60	3.6379788E-12	41.249996	2
>>END_MODULE