FastQCFastQC Report
Sat 18 Jun 2016
SRR3551193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160910
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115170.9920665684678399No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC40860.35196526862547484RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG33580.2892558423994969No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC31650.2726309533038737No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC18590.160132999112765Illumina PCR Primer Index 11 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC16300.14040709443453844Illumina PCR Primer Index 11 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT15610.13446348123454877RNA PCR Primer, Index 41 (96% over 27bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT15370.1323961375128132RNA PCR Primer, Index 41 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC14340.12352378737369822RNA PCR Primer, Index 41 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG11990.10328104676503777RNA PCR Primer, Index 41 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG253.8908307E-545.01
ATACCGG406.8157533E-945.02
TCGAACG302.1655214E-644.9999961
CGTTTTT58400.043.497431
TAGGGCG2000.040.55
TCGACGT451.9283107E-840.026
TAATGCG451.9283107E-840.01
TAAACGG800.039.3752
TACGGGA4050.038.888894
ATAACGG659.094947E-1238.0769272
CGATGAA4050.037.7777819
GTGGCGT900.037.534
CTAACGG900.037.52
AATTTCG301.139929E-437.49999639
TAGGTCG301.139929E-437.4999961
GTTAGCG601.5643309E-1037.4999961
CTACGTC301.139929E-437.4999962
CGTAAGG1950.036.9230772
TCGGCGT1100.036.818184
ACGATAG552.748493E-936.818181