Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551193_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1160910 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 11517 | 0.9920665684678399 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC | 4086 | 0.35196526862547484 | RNA PCR Primer, Index 41 (95% over 23bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG | 3358 | 0.2892558423994969 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC | 3165 | 0.2726309533038737 | No Hit |
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC | 1859 | 0.160132999112765 | Illumina PCR Primer Index 11 (96% over 25bp) |
CGTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC | 1630 | 0.14040709443453844 | Illumina PCR Primer Index 11 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT | 1561 | 0.13446348123454877 | RNA PCR Primer, Index 41 (96% over 27bp) |
CGTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT | 1537 | 0.1323961375128132 | RNA PCR Primer, Index 41 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC | 1434 | 0.12352378737369822 | RNA PCR Primer, Index 41 (96% over 26bp) |
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG | 1199 | 0.10328104676503777 | RNA PCR Primer, Index 41 (96% over 25bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGCGTG | 25 | 3.8908307E-5 | 45.0 | 1 |
ATACCGG | 40 | 6.8157533E-9 | 45.0 | 2 |
TCGAACG | 30 | 2.1655214E-6 | 44.999996 | 1 |
CGTTTTT | 5840 | 0.0 | 43.49743 | 1 |
TAGGGCG | 200 | 0.0 | 40.5 | 5 |
TCGACGT | 45 | 1.9283107E-8 | 40.0 | 26 |
TAATGCG | 45 | 1.9283107E-8 | 40.0 | 1 |
TAAACGG | 80 | 0.0 | 39.375 | 2 |
TACGGGA | 405 | 0.0 | 38.88889 | 4 |
ATAACGG | 65 | 9.094947E-12 | 38.076927 | 2 |
CGATGAA | 405 | 0.0 | 37.77778 | 19 |
GTGGCGT | 90 | 0.0 | 37.5 | 34 |
CTAACGG | 90 | 0.0 | 37.5 | 2 |
AATTTCG | 30 | 1.139929E-4 | 37.499996 | 39 |
TAGGTCG | 30 | 1.139929E-4 | 37.499996 | 1 |
GTTAGCG | 60 | 1.5643309E-10 | 37.499996 | 1 |
CTACGTC | 30 | 1.139929E-4 | 37.499996 | 2 |
CGTAAGG | 195 | 0.0 | 36.923077 | 2 |
TCGGCGT | 110 | 0.0 | 36.81818 | 4 |
ACGATAG | 55 | 2.748493E-9 | 36.81818 | 1 |