##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1160910
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37686986932665	33.0	31.0	34.0	30.0	34.0
2	32.50288825145791	34.0	31.0	34.0	31.0	34.0
3	32.49016547363706	34.0	31.0	34.0	31.0	34.0
4	36.00052372707617	37.0	35.0	37.0	35.0	37.0
5	36.03920200532341	37.0	35.0	37.0	35.0	37.0
6	36.065188515905625	37.0	35.0	37.0	35.0	37.0
7	36.225385258116475	37.0	37.0	37.0	35.0	37.0
8	35.953909433117126	37.0	37.0	37.0	35.0	37.0
9	37.853189308387385	39.0	38.0	39.0	35.0	39.0
10	37.66973753348666	39.0	37.0	39.0	35.0	39.0
11	37.59492725534279	39.0	37.0	39.0	35.0	39.0
12	37.49746061279514	39.0	37.0	39.0	35.0	39.0
13	37.46238123541015	39.0	37.0	39.0	35.0	39.0
14	38.66233213599676	40.0	38.0	41.0	35.0	41.0
15	38.73677029227072	40.0	38.0	41.0	35.0	41.0
16	38.843430584627576	40.0	38.0	41.0	35.0	41.0
17	38.853195338139905	40.0	38.0	41.0	35.0	41.0
18	38.774616464669954	40.0	38.0	41.0	35.0	41.0
19	38.69224401547062	40.0	37.0	41.0	35.0	41.0
20	38.5293838454316	40.0	37.0	41.0	35.0	41.0
21	38.50646734027616	40.0	37.0	41.0	35.0	41.0
22	38.49916014161305	40.0	37.0	41.0	35.0	41.0
23	38.46443479684041	40.0	37.0	41.0	35.0	41.0
24	38.41589701182693	40.0	37.0	41.0	35.0	41.0
25	38.36886063519136	40.0	37.0	41.0	35.0	41.0
26	38.34987466728687	40.0	37.0	41.0	35.0	41.0
27	38.2923025902094	40.0	37.0	41.0	35.0	41.0
28	38.23850169263767	40.0	37.0	41.0	34.0	41.0
29	38.20124385180591	40.0	37.0	41.0	34.0	41.0
30	38.085249502545416	40.0	36.0	41.0	34.0	41.0
31	37.96385077223902	40.0	36.0	41.0	34.0	41.0
32	37.7589606429439	40.0	36.0	41.0	33.0	41.0
33	37.57693447381795	40.0	36.0	41.0	33.0	41.0
34	37.36859274190075	40.0	36.0	41.0	33.0	41.0
35	37.23666864787106	40.0	36.0	41.0	32.0	41.0
36	37.174148728152915	40.0	36.0	41.0	32.0	41.0
37	37.11895065078257	40.0	36.0	41.0	32.0	41.0
38	37.05619384792964	40.0	35.0	41.0	32.0	41.0
39	36.98989241198715	40.0	35.0	41.0	31.0	41.0
40	36.902292167351476	40.0	35.0	41.0	31.0	41.0
41	36.80790500555599	40.0	35.0	41.0	31.0	41.0
42	36.78801715895289	40.0	35.0	41.0	31.0	41.0
43	36.729517361380296	40.0	35.0	41.0	31.0	41.0
44	36.684848093306115	40.0	35.0	41.0	31.0	41.0
45	36.62718040158152	40.0	35.0	41.0	31.0	41.0
46	36.519370149279446	40.0	35.0	41.0	31.0	41.0
47	36.456086173777464	39.0	35.0	41.0	30.0	41.0
48	36.40257384293356	39.0	35.0	41.0	30.0	41.0
49	36.3398850901448	39.0	35.0	41.0	30.0	41.0
50	36.26959109663971	39.0	35.0	41.0	30.0	41.0
51	35.50885770645442	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	14.0
10	12.0
11	12.0
12	19.0
13	29.0
14	39.0
15	44.0
16	69.0
17	105.0
18	215.0
19	394.0
20	684.0
21	1372.0
22	2166.0
23	3391.0
24	5208.0
25	8551.0
26	12874.0
27	14586.0
28	14248.0
29	13671.0
30	14456.0
31	16849.0
32	21160.0
33	29407.0
34	56802.0
35	75588.0
36	67746.0
37	99866.0
38	194395.0
39	505943.0
40	989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.976570104486996	21.24316269133697	23.494500004306968	15.285767199869069
2	35.617403588564144	24.680121628722297	22.91254274663841	16.789932036075147
3	30.42139356194709	24.364334875227193	28.791896012610795	16.42237555021492
4	26.929133180005337	24.601734845939824	30.432074837842727	18.03705713621211
5	25.433409997329683	28.199257479046608	27.365945680543714	19.00138684308
6	24.166989689123188	33.2174759455944	27.467848498160922	15.147685867121483
7	76.777441834423	5.604741108268514	13.037444763159936	4.580372294148556
8	78.62547484301109	5.759188912146506	11.279168927823862	4.336167317018546
9	72.64439103806497	6.887786305570629	13.253568321403037	7.214254334961366
10	38.25352525174217	27.964097130699194	20.42337476634709	13.35900285121155
11	27.197026470613572	24.469080290461793	29.947971849669656	18.38592138925498
12	25.36234505689502	21.89032741556193	32.411728730047976	20.33559879749507
13	23.729660352654385	22.492355135195666	34.720262552652656	19.05772195949729
14	19.56878655537466	26.787864692353413	33.39216649008105	20.251182262190866
15	18.77570182012387	26.237262147797846	36.24785728437174	18.739178747706543
16	22.74879189601261	25.670809968042313	32.403631633804515	19.176766502140563
17	22.86042845698633	25.769353352111708	31.093538689476357	20.27667950142561
18	22.726309533038737	25.692517077120534	31.706936799579637	19.874236590261088
19	22.920725982203617	27.318396774943793	30.14273285612149	19.6181443867311
20	23.51413976966345	27.79267988043862	30.73356246392916	17.959617885968765
21	22.651196044482347	27.087543392683326	32.06984176206596	18.191418800768364
22	21.476858671214824	24.06853244437553	32.52663858524778	21.927970299161863
23	20.67671051158143	26.498953407240872	32.74741366686479	20.076922414312907
24	20.944259244902703	26.179031966302297	31.717445796831793	21.159262991963203
25	21.245230035058704	27.921199748473178	30.001808925756517	20.831761290711597
26	20.24678915678218	27.45561671447399	30.67688279022491	21.62071133851892
27	21.153836214693644	26.908029046179287	30.799200627094265	21.1389341120328
28	19.430619083305338	27.26068342937868	31.67764943018839	21.631048057127597
29	21.178988896641428	26.06550034025032	31.70280211213617	21.052708650972082
30	21.542755252345145	25.245970833225662	31.111886365006765	22.099387549422435
31	22.30698331481338	25.460802301642676	29.740979059530886	22.491235324013058
32	22.811329043595112	27.029485489831252	29.37557605671413	20.78360940985951
33	22.214211265300495	26.325210395293347	28.372828212350655	23.0877501270555
34	20.7234841632857	26.6793291469623	30.571878957025096	22.025307732726912
35	20.031871549043423	27.32485722407422	29.929451895495774	22.713819331386585
36	20.92367194700709	27.673204641186654	28.676210903515347	22.72691250829091
37	20.875347787511522	28.42632073115056	28.661136522211024	22.03719495912689
38	21.1221369442937	27.26740229647432	28.204770395637908	23.40569036359408
39	20.847869343876788	26.76159219922302	28.572413020819877	23.81812543608032
40	21.505284647388688	25.23261923835612	31.130578597824123	22.131517516431074
41	19.369977000801093	26.510582215675633	29.955724388626166	24.163716394897104
42	20.79437682507688	25.915531781102757	30.92780663445056	22.362284759369803
43	21.200351448432695	25.412392002825367	29.80566969015686	23.581586858585077
44	21.66912163733623	25.58983900560767	29.400039624088	23.3409997329681
45	21.02178463446779	25.23210240242568	28.616774771515445	25.129338191591078
46	21.270124299041267	26.080402442911165	28.519954173880834	24.12951908416673
47	19.817470777235098	26.2278729617283	31.528111567649514	22.426544693387086
48	20.401064682016692	26.013472189919977	30.235763323599592	23.34969980446374
49	21.14505000387627	24.645407482061486	30.508997252155634	23.70054526190661
50	19.580329224487684	25.62102144007718	30.326123472103784	24.472525863331352
51	19.668449750626664	25.24993324202565	29.162984210662323	25.91863279668536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	549.0
1	1074.5
2	1600.0
3	10096.0
4	18592.0
5	12194.0
6	5796.0
7	5748.5
8	5701.0
9	5709.5
10	5718.0
11	5681.0
12	5644.0
13	5464.0
14	5284.0
15	5136.0
16	4988.0
17	4910.0
18	4832.0
19	4662.5
20	4493.0
21	4754.0
22	5015.0
23	5717.0
24	6419.0
25	7310.0
26	10170.5
27	12140.0
28	13965.0
29	15790.0
30	18120.0
31	20450.0
32	22685.5
33	24921.0
34	29419.0
35	33917.0
36	37366.0
37	40815.0
38	43857.5
39	46900.0
40	50070.0
41	53240.0
42	58477.5
43	63715.0
44	69377.5
45	75040.0
46	90634.0
47	106228.0
48	109516.0
49	112804.0
50	109533.0
51	106262.0
52	95679.0
53	85096.0
54	74536.5
55	63977.0
56	58266.0
57	52555.0
58	48728.0
59	44901.0
60	40856.0
61	36811.0
62	33566.5
63	30322.0
64	25180.0
65	20038.0
66	16147.5
67	12257.0
68	10559.0
69	8861.0
70	6793.0
71	4725.0
72	3681.0
73	2637.0
74	2207.0
75	1453.5
76	1130.0
77	841.0
78	552.0
79	352.0
80	152.0
81	100.5
82	49.0
83	28.0
84	7.0
85	5.5
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1160910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.24324784177787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1253185748082	22.115471331668875
2	9.739388636118806	5.891014886990719
3	3.7091748206146824	3.3653248016499546
4	2.026013556700702	2.4509292050440483
5	1.2917592547726258	1.9533497646999403
6	0.968748919731072	1.7578868205528835
7	0.7490310893406977	1.585719301328931
8	0.6078930490426926	1.4707728114793743
9	0.5622054585031712	1.5302627117560592
>10	6.825071917081601	45.94775880647577
>50	0.34867482919303894	6.37612935307224
>100	0.039798428303517534	1.9586086273366659
>500	0.0037491273025136718	0.7731166222149488
>1k	0.0028839440788566706	1.819144109830459
>5k	0.0	0.0
>10k+	2.8839440788566706E-4	1.0045108458990937
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11517	0.9920665684678399	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	4086	0.35196526862547484	RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	3358	0.2892558423994969	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	3165	0.2726309533038737	No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1859	0.160132999112765	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	1630	0.14040709443453844	Illumina PCR Primer Index 11 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	1561	0.13446348123454877	RNA PCR Primer, Index 41 (96% over 27bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT	1537	0.1323961375128132	RNA PCR Primer, Index 41 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1434	0.12352378737369822	RNA PCR Primer, Index 41 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1199	0.10328104676503777	RNA PCR Primer, Index 41 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7227864347796126E-4	0.0	0.0	0.13842589003454187	0.0
2	1.7227864347796126E-4	0.0	0.0	0.5174389056860567	0.0
3	1.7227864347796126E-4	0.0	0.0	0.7979085372681776	0.0
4	1.7227864347796126E-4	0.0	0.0	1.2068980368848576	0.0
5	1.7227864347796126E-4	0.0	0.0	2.5054483120999906	0.0
6	1.7227864347796126E-4	0.0	0.0	3.5413597953329714	0.0
7	1.7227864347796126E-4	0.0	0.0	4.372862668079351	0.0
8	1.7227864347796126E-4	0.0	0.0	5.419713845173183	0.0
9	1.7227864347796126E-4	0.0	0.0	6.0443962064242704	0.0
10	1.7227864347796126E-4	0.0	0.0	7.314692784109018	0.0
11	1.7227864347796126E-4	0.0	0.0	8.482655847567857	0.0
12	1.7227864347796126E-4	0.0	0.0	9.681629066852727	0.0
13	1.7227864347796126E-4	0.0	0.0	10.138339750712802	0.0
14	1.7227864347796126E-4	0.0	0.0	10.410281589442764	0.0
15	1.7227864347796126E-4	0.0	0.0	10.727963408016125	0.0
16	1.7227864347796126E-4	0.0	0.0	11.203710881980514	0.0
17	1.7227864347796126E-4	0.0	0.0	11.780327501701251	0.0
18	1.7227864347796126E-4	0.0	0.0	12.416035696134928	0.0
19	1.7227864347796126E-4	0.0	0.0	12.914007115107976	0.0
20	1.7227864347796126E-4	0.0	0.0	13.349269107855045	0.0
21	1.7227864347796126E-4	0.0	0.0	13.860247564410678	0.0
22	1.7227864347796126E-4	0.0	0.0	14.420756130966225	0.0
23	1.7227864347796126E-4	0.0	0.0	14.964122972495714	0.0
24	1.7227864347796126E-4	0.0	0.0	15.390512615103669	0.0
25	1.7227864347796126E-4	0.0	0.0	15.778828677502993	0.0
26	1.7227864347796126E-4	0.0	0.0	16.150433711484954	0.0
27	1.7227864347796126E-4	0.0	0.0	16.5675203073451	0.0
28	1.7227864347796126E-4	0.0	0.0	16.966776063605277	0.0
29	1.7227864347796126E-4	0.0	0.0	17.396180582474095	0.0
30	1.7227864347796126E-4	0.0	0.0	17.922319559655786	0.0
31	1.7227864347796126E-4	0.0	0.0	18.408489891550595	0.0
32	1.7227864347796126E-4	0.0	0.0	18.87708780181065	0.0
33	1.7227864347796126E-4	0.0	0.0	19.298309085114266	0.0
34	1.7227864347796126E-4	0.0	0.0	19.70592035558312	0.0
35	1.7227864347796126E-4	0.0	0.0	20.140751651721494	0.0
36	1.7227864347796126E-4	0.0	0.0	20.552411470312084	0.0
37	1.7227864347796126E-4	0.0	0.0	20.99861315692	0.0
38	1.7227864347796126E-4	0.0	0.0	21.475566581388737	0.0
39	1.7227864347796126E-4	0.0	0.0	22.16562868783971	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTG	25	3.8908307E-5	45.0	1
ATACCGG	40	6.8157533E-9	45.0	2
TCGAACG	30	2.1655214E-6	44.999996	1
CGTTTTT	5840	0.0	43.49743	1
TAGGGCG	200	0.0	40.5	5
TCGACGT	45	1.9283107E-8	40.0	26
TAATGCG	45	1.9283107E-8	40.0	1
TAAACGG	80	0.0	39.375	2
TACGGGA	405	0.0	38.88889	4
ATAACGG	65	9.094947E-12	38.076927	2
CGATGAA	405	0.0	37.77778	19
GTGGCGT	90	0.0	37.5	34
CTAACGG	90	0.0	37.5	2
AATTTCG	30	1.139929E-4	37.499996	39
TAGGTCG	30	1.139929E-4	37.499996	1
GTTAGCG	60	1.5643309E-10	37.499996	1
CTACGTC	30	1.139929E-4	37.499996	2
CGTAAGG	195	0.0	36.923077	2
TCGGCGT	110	0.0	36.81818	4
ACGATAG	55	2.748493E-9	36.81818	1
>>END_MODULE