FastQCFastQC Report
Sat 18 Jun 2016
SRR3551192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653406
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC22350.34205379197619856No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC17680.2705821495364291No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG16900.2586447017627631No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC16110.24655420978687065No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.1700321086736271No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT8610.13177105811700537Illumina Single End Adapter 1 (95% over 21bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA7690.11769099151216855No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC7580.1160075052876772No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7010.10728398576076742No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6920.10590658794072903No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTA453.8380676E-1045.00000413
TGTTTCG207.0310506E-445.042
AACCGAT207.0310506E-445.033
AATCCGT207.0310506E-445.010
GTCGACC207.0310506E-445.040
CCGATTT207.0310506E-445.040
CCGATAT207.0310506E-445.010
TAGCGCG207.0310506E-445.01
TCGGGAT551.8189894E-1245.05
ATTCGCA207.0310506E-445.044
ATTCGAT207.0310506E-445.011
TTATCGC207.0310506E-445.031
CCGTATA207.0310506E-445.013
TCAATCA207.0310506E-445.028
ATTCACG253.888952E-545.040
GTATGCG207.0310506E-445.01
TCGTCGA253.888952E-545.026
ACGTCGA207.0310506E-445.017
CGTAACA207.0310506E-445.015
CGTCGAT253.888952E-545.027