##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	653406
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.348862728533256	33.0	31.0	34.0	30.0	34.0
2	32.44163200215486	34.0	31.0	34.0	30.0	34.0
3	32.47351876168875	34.0	31.0	34.0	30.0	34.0
4	35.957505746809794	37.0	35.0	37.0	35.0	37.0
5	36.00124731024815	37.0	35.0	37.0	35.0	37.0
6	36.0411398120005	37.0	35.0	37.0	35.0	37.0
7	36.18693584080954	37.0	37.0	37.0	35.0	37.0
8	35.86518336225869	37.0	36.0	37.0	35.0	37.0
9	37.74588540662314	39.0	38.0	39.0	35.0	39.0
10	37.614852021560864	39.0	37.0	39.0	35.0	39.0
11	37.53419772698751	39.0	37.0	39.0	35.0	39.0
12	37.33478419237044	39.0	37.0	39.0	35.0	39.0
13	37.22758591136292	39.0	37.0	39.0	34.0	39.0
14	38.450145851124724	40.0	38.0	41.0	34.0	41.0
15	38.519813102420244	40.0	38.0	41.0	35.0	41.0
16	38.61235280973851	40.0	38.0	41.0	35.0	41.0
17	38.61845162119723	40.0	38.0	41.0	35.0	41.0
18	38.61036629599361	40.0	38.0	41.0	35.0	41.0
19	38.60615758043238	40.0	37.0	41.0	35.0	41.0
20	38.518423461064025	40.0	37.0	41.0	35.0	41.0
21	38.491313211081625	40.0	37.0	41.0	35.0	41.0
22	38.452778211403015	40.0	37.0	41.0	35.0	41.0
23	38.4191987829925	40.0	37.0	41.0	34.0	41.0
24	38.361081777639015	40.0	37.0	41.0	34.0	41.0
25	38.300617074223375	40.0	37.0	41.0	34.0	41.0
26	38.27813028959024	40.0	37.0	41.0	34.0	41.0
27	38.21327933933879	40.0	37.0	41.0	34.0	41.0
28	38.16530304282483	40.0	36.0	41.0	34.0	41.0
29	38.16616468168336	40.0	36.0	41.0	34.0	41.0
30	38.077267426378086	40.0	36.0	41.0	34.0	41.0
31	38.0074165220399	40.0	36.0	41.0	34.0	41.0
32	37.918811581160874	40.0	36.0	41.0	34.0	41.0
33	37.85984824136907	40.0	36.0	41.0	34.0	41.0
34	37.79436215767838	40.0	36.0	41.0	34.0	41.0
35	37.72750326749372	40.0	36.0	41.0	33.0	41.0
36	37.649844047957934	40.0	35.0	41.0	33.0	41.0
37	37.58879777657383	40.0	35.0	41.0	33.0	41.0
38	37.49737682237384	40.0	35.0	41.0	33.0	41.0
39	37.45371637236266	40.0	35.0	41.0	33.0	41.0
40	37.375114400541165	40.0	35.0	41.0	33.0	41.0
41	37.29385252048497	40.0	35.0	41.0	33.0	41.0
42	37.26200096111759	40.0	35.0	41.0	33.0	41.0
43	37.167955298849414	39.0	35.0	41.0	33.0	41.0
44	37.11195183392868	39.0	35.0	41.0	33.0	41.0
45	37.05535761838734	39.0	35.0	41.0	32.0	41.0
46	36.92327588053982	39.0	35.0	41.0	32.0	41.0
47	36.85899272427863	39.0	35.0	41.0	32.0	41.0
48	36.812776436090275	39.0	35.0	41.0	32.0	41.0
49	36.69846313012124	39.0	35.0	41.0	32.0	41.0
50	36.59260551632522	39.0	35.0	41.0	32.0	41.0
51	35.82591068952535	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	6.0
10	8.0
11	13.0
12	12.0
13	12.0
14	25.0
15	30.0
16	43.0
17	66.0
18	118.0
19	207.0
20	371.0
21	627.0
22	971.0
23	1410.0
24	2080.0
25	2888.0
26	3900.0
27	4634.0
28	5376.0
29	6041.0
30	7411.0
31	9544.0
32	12443.0
33	17904.0
34	36931.0
35	58737.0
36	39718.0
37	60106.0
38	112697.0
39	268434.0
40	635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.028206046470345	20.83222376286719	22.842153270707648	11.297416919954822
2	32.54546178027138	25.4928788532704	24.059925987823803	17.901733378634418
3	33.00796135939982	24.79117118606196	24.94390929988399	17.256958154654228
4	29.512737868951312	24.641034823677774	26.12311487803908	19.72311242933184
5	26.069090274653124	30.199141115937106	23.414691631236934	20.317076978172835
6	26.322378429337963	36.00257726436549	22.608760862312252	15.066283443984291
7	79.51656397400697	6.634925299124893	9.384823524730413	4.463687202137721
8	79.39045555137236	6.914230968188233	8.452019112159974	5.243294368279447
9	73.36434008870441	8.025178832150303	10.631521596067376	7.978959483077902
10	42.63918604971488	25.28856484329804	17.54360994542444	14.528639161562642
11	32.058781217191154	25.11302314334426	23.115031083277472	19.713164556187117
12	30.805349200956222	22.84674459677444	26.20086133277013	20.147044869499208
13	25.94390011723186	26.10398435276076	28.33781752845857	19.614298001548807
14	20.31677088976838	29.485955133561674	28.006017698031542	22.191256278638395
15	19.449622439953107	26.476953073586714	34.5904384104217	19.48298607603848
16	22.28966370067003	25.82406650688852	30.497883398683207	21.388386393758246
17	22.496732506282466	25.436864675255507	27.19381211681558	24.87259070164645
18	23.545850512545034	25.76483840062687	29.20955730434064	21.479753782487457
19	25.13735717149827	27.338438887919608	26.551332555868782	20.972871384713333
20	26.368750822612586	27.07612112530341	27.365221623309242	19.18990642877476
21	25.948491443298654	27.182639890053046	27.30538134023869	19.563487326409614
22	23.052129916162386	25.266220389772975	27.09188467813274	24.589765015931903
23	22.656969786013597	26.786714538893126	27.849147390749394	22.707168284343883
24	23.748940168899583	25.575369678270476	28.856484329804132	21.819205823025804
25	22.489692472980046	27.132900524329433	26.86430794942195	23.513099053268565
26	21.964903903545423	27.957196597521296	27.054388848587248	23.023510650346033
27	22.207478964074404	28.400871739775884	28.067388423124367	21.324260873025345
28	20.68254653308969	27.850524788569437	29.323116102392692	22.143812575948186
29	22.960609483230947	26.36293514292798	28.016424703782945	22.660030670058127
30	23.322405977294363	28.28455814608375	26.713865498633314	21.679170377988573
31	24.614864265097044	27.936382586018492	24.206542333556776	23.24221081532768
32	24.900597790653897	27.69411361389397	25.84671704881804	21.5585715466341
33	23.872446840096355	28.46285464167761	25.161232067045603	22.50346645118043
34	22.24604610303548	27.059745395665175	27.39583046375454	23.298378037544804
35	24.622363431006143	26.621732888892968	26.39430920438441	22.361594475716476
36	24.968243328038003	29.429328778737872	25.21678711245382	20.385640780770302
37	23.34077128156154	28.07595889844905	27.836444721964597	20.746825098024814
38	21.94408989204262	29.442949712736034	26.279066920107862	22.33389347511348
39	22.970404312173443	26.10934089983869	27.93347474617619	22.986780041811674
40	24.204399714725607	25.304940572936275	27.904090259348706	22.586569452989412
41	21.523065291717554	25.805089025812432	27.420011447706326	25.251834234763688
42	24.02135884886273	25.40763323263025	27.81685506407961	22.754152854427414
43	24.10966535354741	25.28075958898449	27.931179083142794	22.678395974325305
44	22.50897604246058	26.45797559251063	27.043828798633623	23.989219566395168
45	23.058863861060352	25.432579437593166	26.303553992464103	25.20500270888238
46	23.48356152223885	27.552241638430015	26.431345901323223	22.532850938007915
47	22.255228755169068	25.633985607723226	29.678637784164824	22.432147852942887
48	22.3946520233974	24.485848002620116	29.293731615565203	23.825768358417278
49	22.19370498587402	24.96273373675785	30.520533940612726	22.323027336755402
50	21.191418505492756	24.60598770136791	29.640682822012653	24.56191097112668
51	21.476080721634023	24.1211436687144	27.52316324000698	26.8796123696446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	320.0
1	761.5
2	1203.0
3	1870.5
4	2538.0
5	1876.5
6	1215.0
7	1168.5
8	1122.0
9	1151.5
10	1181.0
11	1144.0
12	1107.0
13	1179.0
14	1251.0
15	1192.5
16	1134.0
17	1175.0
18	1216.0
19	1222.0
20	1228.0
21	1579.5
22	1931.0
23	2127.0
24	2323.0
25	2940.0
26	4481.5
27	5406.0
28	6558.0
29	7710.0
30	8458.0
31	9206.0
32	10877.0
33	12548.0
34	14053.0
35	15558.0
36	16836.5
37	18115.0
38	19977.5
39	21840.0
40	25870.5
41	29901.0
42	32857.5
43	35814.0
44	40398.5
45	44983.0
46	53917.0
47	62851.0
48	63331.0
49	63811.0
50	62735.0
51	61659.0
52	56724.5
53	51790.0
54	46410.5
55	41031.0
56	36484.5
57	31938.0
58	30453.0
59	28968.0
60	27111.5
61	25255.0
62	22461.5
63	19668.0
64	17583.0
65	15498.0
66	13184.0
67	10870.0
68	8938.5
69	7007.0
70	5680.0
71	4353.0
72	3565.0
73	2777.0
74	2192.5
75	1331.0
76	1054.0
77	779.5
78	505.0
79	379.0
80	253.0
81	151.5
82	50.0
83	48.0
84	46.0
85	24.0
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	653406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.556673332228936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.63699832819286	17.346096931146334
2	11.608312926193722	5.7012309693365975
3	4.444935094156147	3.274584572704583
4	2.1857334357809317	2.146973678952109
5	1.3329023400650526	1.636582367437051
6	0.874854226547287	1.2890105672784922
7	0.6083470023624697	1.0457285026759113
8	0.47892380825210207	0.9408620408219135
9	0.4176807746481679	0.923116530616865
>10	5.9097126310013035	37.96133666389452
>50	1.4026690463334743	21.525422915734534
>100	0.08884830886478881	3.793115363123621
>500	0.006931428351153736	1.1138108099279493
>1k	0.0031506492505244256	1.302128086349499
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	2235	0.34205379197619856	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC	1768	0.2705821495364291	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG	1690	0.2586447017627631	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	1611	0.24655420978687065	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1111	0.1700321086736271	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	861	0.13177105811700537	Illumina Single End Adapter 1 (95% over 21bp)
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	769	0.11769099151216855	No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	758	0.1160075052876772	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	701	0.10728398576076742	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	692	0.10590658794072903	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5304420222648704E-4	0.0	0.0	0.13773978200383835	0.0
2	1.5304420222648704E-4	0.0	0.0	0.7828210943884812	0.0
3	1.5304420222648704E-4	0.0	0.0	1.0543215091382694	0.0
4	1.5304420222648704E-4	0.0	0.0	1.4722852254188055	0.0
5	1.5304420222648704E-4	0.0	0.0	2.7854044805220646	0.0
6	1.5304420222648704E-4	0.0	0.0	3.4390562682313908	0.0
7	1.5304420222648704E-4	0.0	0.0	4.059803552462022	0.0
8	1.5304420222648704E-4	0.0	0.0	5.055049999540867	0.0
9	1.5304420222648704E-4	0.0	0.0	5.4528118811275075	0.0
10	1.5304420222648704E-4	0.0	0.0	6.643495774449576	0.0
11	1.5304420222648704E-4	0.0	0.0	7.9068756638292275	0.0
12	1.5304420222648704E-4	0.0	0.0	9.128168397596594	0.0
13	1.5304420222648704E-4	0.0	0.0	9.536796417541314	0.0
14	1.5304420222648704E-4	0.0	0.0	9.70422677477709	0.0
15	1.5304420222648704E-4	0.0	0.0	10.06939024128949	0.0
16	1.5304420222648704E-4	0.0	0.0	10.767883980251176	0.0
17	1.5304420222648704E-4	0.0	0.0	11.553459870279735	0.0
18	1.5304420222648704E-4	0.0	0.0	12.4633076525162	0.0
19	1.5304420222648704E-4	0.0	0.0	13.017327664576083	0.0
20	1.5304420222648704E-4	0.0	0.0	13.546248427470822	0.0
21	1.5304420222648704E-4	0.0	0.0	14.180769689901837	0.0
22	1.5304420222648704E-4	0.0	0.0	14.83977802468909	0.0
23	1.5304420222648704E-4	0.0	0.0	15.477819303771316	0.0
24	1.5304420222648704E-4	0.0	0.0	15.98255908271427	0.0
25	1.5304420222648704E-4	0.0	0.0	16.44505866184271	0.0
26	1.5304420222648704E-4	0.0	0.0	16.852003195562943	0.0
27	1.5304420222648704E-4	0.0	0.0	17.257264243058682	0.0
28	1.5304420222648704E-4	0.0	0.0	17.673697517316953	0.0
29	1.5304420222648704E-4	0.0	0.0	18.112781333504742	0.0
30	1.5304420222648704E-4	0.0	0.0	18.624255057345664	0.0
31	1.5304420222648704E-4	0.0	0.0	19.111088664628117	0.0
32	1.5304420222648704E-4	0.0	0.0	19.590423106001474	0.0
33	1.5304420222648704E-4	0.0	0.0	20.052157464118785	0.0
34	3.060884044529741E-4	0.0	0.0	20.50562743531587	0.0
35	4.591326066794612E-4	0.0	0.0	20.972718340511108	0.0
36	4.591326066794612E-4	0.0	0.0	21.419148278405768	0.0
37	4.591326066794612E-4	0.0	0.0	21.881188724927533	0.0
38	6.121768089059482E-4	0.0	0.0	22.33389347511348	0.0
39	6.121768089059482E-4	0.0	0.0	22.832511485967377	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTA	45	3.8380676E-10	45.000004	13
TGTTTCG	20	7.0310506E-4	45.0	42
AACCGAT	20	7.0310506E-4	45.0	33
AATCCGT	20	7.0310506E-4	45.0	10
GTCGACC	20	7.0310506E-4	45.0	40
CCGATTT	20	7.0310506E-4	45.0	40
CCGATAT	20	7.0310506E-4	45.0	10
TAGCGCG	20	7.0310506E-4	45.0	1
TCGGGAT	55	1.8189894E-12	45.0	5
ATTCGCA	20	7.0310506E-4	45.0	44
ATTCGAT	20	7.0310506E-4	45.0	11
TTATCGC	20	7.0310506E-4	45.0	31
CCGTATA	20	7.0310506E-4	45.0	13
TCAATCA	20	7.0310506E-4	45.0	28
ATTCACG	25	3.888952E-5	45.0	40
GTATGCG	20	7.0310506E-4	45.0	1
TCGTCGA	25	3.888952E-5	45.0	26
ACGTCGA	20	7.0310506E-4	45.0	17
CGTAACA	20	7.0310506E-4	45.0	15
CGTCGAT	25	3.888952E-5	45.0	27
>>END_MODULE